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'!! %"5.("##% '  #,'"$)'  "%$'" ,+."%5%!.0! )!*(!&$*4! #-$"& %")-&!$5!".#%  # *#*. ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 319bbc4025fa4248a33f79fef1f8f526Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-WellK1363_H_6_Vicia_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7ziox+LkLrX]1xE o  ~ T SJ0 U#g&*.27*;@FXLPRY`h9oWv*|zsLkd\UMNHC>:62/-,Z+,-/;137589;=@C(E-FH*IHJ%JJIGEB?^<85C1.*X&"g]'!#'~R<pSn^A  3 1*v1V (Hm_|D G{ln~&Du {E "4")1:EgSgpYI_=5X.n(y#jh3E;a  o |  v  >>f 7 F y D7SJ5kX *  $fqE5>[E F J   C +O{"M@weP<,!PZ3"yH 6UcV/0:5b "7FPYh |iQ3 /;BEEB;.UNAq}R 5] |snllmnonnlkjhd`[WVZcnz~vqpsx}~ym\J<45>Naw` O[5  "I,a]'Q'  )5=@@>94-% '00)    `|OJ> %))& !#&-8FSXTE1     6O`dYE/$19<6*   ')$  "$"  )7>;0   K]1lO !"09;5) /Nl{nfdgkptz#7IXbeb\XYan{tifinql\C$|sqrqhXC1%#)3;>90%#)/5;>=91' *7CJJB2 !-21*"!#! !$-23.'!      4z@TB  $! 6q'p Y#     (/1.'  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(00'  L~BFA4vQ !#"#T!go.+!ncG 6 #*06:<:3(   &&! 4#TtZ^ *24/% !%$   4w`jn$    -p:u J  KX'c#   ?/`HK1p\CJ#&&&'('$  -9=8+  $lZ-DF<9} }|0         ; (f"0"Z=$.2/'&+,*&#  !  !!          6~MlJO.w[.Q(0+mU'#}u/ "'**'""&&J8=ZO4@)T 'gJG]cq0!6JQJ8# K&SP'X ',,'  #.562) &&,{c$._ $08<><7.$ $('"         "a9HB*_%  +10)   #,6>DGFB<5+  /s'D   !"%,6ALSTPH>5*6Re:_w-(3750)""$$%)0;GPSK;(>_Cb<jN=3,'&&''# 5MdxpYG?BO]geWB- 7*W?4j|rXD4'#7Rq^@*#*-*")A\r~~pX<"                                 $%!""!-5983-)(*-0345430../269;:98789<@EJMMIC:3.-/01/+'%',39>>91(                                                                                                                                                                                                                       vFSAAL0AQ?A KB.bcpKB_3730_POP7_BDTv3.mobtermCTTTAGTAAAAGCTAGTATTCTGTCGTCTGGCTCTCCGCCCTAGAACTATGTCTAAATTACACTATGTCTAAATTACATAACTTTTGATACCTTTTCATTATTCTTTTCTTCGAAGTTCTTACGAATCTTCTAAAATTATTAAAAAAAAAGAATAAGAAAACTCAAAATTTACAAATTGATTTTGGTGAAATAAAGAATGCTAATGTTAAAGAATATGGAAGTTAATAAAGAATTTATTAAATTCTGTATATGTCTACTGAAAAAGAATACTAACAAAAAGTAAGGGAGTAATATCCACAATGTTGATATTACTCCTTTACTTTCAGAAACTCGTATCCTTAAACCAAAATATTATCCATTTATAGATGGAGCCTCGACCGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCAGCGACTTTAGTAAAAGCTAGTATTCTGTCGTCTGGCTCTCCGCCCTAGAACTATGTCTAAATTACACTATGTCTAAATTACATAACTTTTGATACCTTTTCATTATTCTTTTCTTCGAAGTTCTTACGAATCTTCTAAAATTATTAAAAAAAAAGAATAAGAAAACTCAAAATTTACAAATTGATTTTGGTGAAATAAAGAATGCTAATGTTAAAGAATATGGAAGTTAATAAAGAATTTATTAAATTCTGTATATGTCTACTGAAAAAGAATACTAACAAAAAGTAAGGGAGTAATATCCACAATGTTGATATTACTCCTTTACTTTCAGAAACTCGTATCCTTAAACCAAAATATTATCCATTTATAGATGGAGCCTCGACCGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCAGCGA!,8Tjq|)8I_en !+8DL[ck}(1<JTbm})7DQ]ju&3@MYdq} #/;GTalz(3?JVcoz!,9EP\gr~  " - 8 C N Z f r     ' 3 ? 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K V a l y  " . 9 E Q \ h t    ' 3 @ K W c p }   ! , 7 C P \ h t  (3>JVbn{ %1=GT`my ,9EP\ht(4@KWdp{'3>KXdo|%1<IVbnz ".:FR`o} #0   '"'5>>> #+!/%)/)/>&>>>/88'+.8>>>>  %>>;;>>>>>>>>>>;>666>>;>>>>  88+ !0;;>>>>>>>>>>>>>>>;>>>;>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>;66682$1.12>68>;>>>>>>>>>>>>>>;;8;;;>>>>>>>>>>;2;8;>;668>>6;>>>>>>>>>;>>>>>>>>>>>>>>>>;>>;>>>>>>>>>>8>;>>;>>>>>>>>>>>>;>>>>;;8>;;;>>;>>>>>>>>>>>>>>>>;>>>>>>>>>>>;;;;>;>>+8>>>>"&#-.-&$)8832&%8838>23833#&&) 28&)  #0   '"'5>>> #+!/%)/)/>&>>>/88'+.8>>>>  %>>;;>>>>>>>>>>;>666>>;>>>>  88+ !0;;>>>>>>>>>>>>>>>;>>>;>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>>>>>;66682$1.12>68>;>>>>>>>>>>>>>>;;8;;;>>>>>>>>>>;2;8;>;668>>6;>>>>>>>>>;>>>>>>>>>>>>>>>>;>>;>>>>>>>>>>8>;>>;>>>>>>>>>>>>;>>>>;;8>;;;>>;>>>>>>>>>>>>>>>>;>>>>>>>>>>>;;;;>;>>+8>>>>"&#-.-&$)8832&%8838>23833#&&) 28&) J5L !N1#-b>5+Q[[I-+M#!!Q(-1+/,+c3 $%+L"c'X^E8 $]Q;*2>$TK4- %S*%[CP8W)REI1X_b\\O1)N_Db YG@)SG( _]cN*5/<8C+A!17Z@D L0]bT&%B+H a%^X$TR 6`I;((+JFXC"#)UBK`/\F B0a `TI/S5,OX`&8\ (J$DL%X/+"I:bU">M)+Q!ELOE1J-@-1'V D$&LR T],-& +OD# %/VU>J)Y/`UQ$&2;M&\X0K[,H XB P/O2Q\ W2!0  c0;+]]9."Z 1W8P/VY+- 6aS2@Y:YN2LZ Y.+c3;\F1EOBR)'?J^'**4[*)\V6*30L8a=;=+;MjcK` xQ: P\_:1>C[vNWA,Y1O%3av:X)Qj#pC+rX{+[iHXh 1A>]lKh` Eo>J?b<P6kh _, `PwA;]Of#> oO |gztlyb* *B aIe66M {ZL~F | man]K4n`0xfuvM 7qD_2hbFaO]<Wa/z*(Z,z,K5h*vHJ$[ Oet EG H 8 `= (RJ x.X^FYZsVW3J?;?*=%C;3H67OE%.'Y*I'2N#$ <  " /  ! 3    +C A.P  x16Kd8&`ap.[pp=+,n^PHKJ;v,|0^"K DSd^vQpK&rDH.tS+= ..8 bLVa&\Cu amWL\^MMZ8 9 IU*2&f>TG J Yu$pt ~6#Y?~q?Z(Bx8p u:8*l&H/Ylx==@ M!73cN:XR02 -U )h_ ' *[1w` )?6/Fi7:Y\A*#.&Mpz>0!)KM;Z p4&3EH]97A!&%2"@"4$;'?1*4VaA|i:G5 0":,%,)&.+3.4#"9'& ':35/L]%C/7")074!%5(1.p@.*/MWH^f\;;'_(- &#.#%2,8"!#.%-"(!Y=X=& ")2"e.5*&>7"D%V*"=C+7C*V$,&@ "0IJ,%+06#&M 7!K2Jv*+AM%<LDRA#,<2=;.1m,*!Eq kQm,!Rraj1PeoT[}&9G:AAAAGAGGGGCCGCGCGGGGACAAACCACTCAAACTGCGATATATCAATGTCTATTGAATACAATGTGGATTTAATACGGGGGGCCCCCCTAAAAAGGGAGGGAGGACTAAAAGGAGGGGACATGCCTCCTCCGGGAGGGGTGTGGTTCCATTCTTTTCTCTCGCTTTCCCCACCTGGTTAACCAAGAGGTATCGAGGAAAGCTCCCCCCGATTATCATGGACGTTATTGAGGAACTCCCTTCGGGAGGGGGTGGGGGGAGGCGGAGCACACCTAGCTGGAGGGAAAGAAGGCTATACAGCGTGGAGATAATACAACCCCACCAGGAGGTACAAGTATTCCTTTGATCGTATAATGTGCACCTATAGAAAGAGACTCGGGAGCAAAGTTGGGTAGAAAGGGAAGGTGTGGGATTTCGGTACATGCCAACAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:56:10 -04:00AEPt3AEPt3APFN APXV2APrN ÇAPrV1APrX}}ÐARTNASPFASPtASPtB1PtB1PtBCTSGBufT"DYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DDATA"DZDATA 'ODATA 'O5DATA 'ODATA 'OշDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~F LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS%OfSc&P1AMb6P1RLb3pP1WDb:4P2AMb@P2BADZP2RLb=PBAS'PBAS%PCON1PCON0PDMFPDMF%PLOCb,PLOCb)JPSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%%SCAN3SMED SMLtSMPLSPACAka?SPAC%SPACAka?SVER4.0SVER GSVER,,žSatd&^Scal@Scan3TUBEH6Tmpr<UserphARphCH%phDYbigphQLcphTRphTR-This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ÇAPrV1APrX}}ÐARTNASPFASPtASPtB1PtB1PtBCTSGBufT"DYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DDATA"DZDATA 'ODATA 'O5DATA 'ODATA 'OշDCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabUFVocUFWO_GATCFeat//UGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE"LAST~~F LIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS%OfSc&P1AMb6P1RLb3pP1WDb:4P2AMb@P2BADZP2RLb=PBAS'PBAS%PCON1PCON0PDMFPDMF%PLOCb,PLOCb)JPSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%%SCAN3SMED SMLtSMPLSPACAka?SPAC%SPACAka?SVER4.0SVER GSVER,,žSatd&^Scal@Scan3TUBEH6Tmpr<UserphARphCH%phDYbigphQLcphTRphTRqk+VxnDS\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4{,R09U!fktNhe7+Dowdo!:Z`+a6:!.tkSY4E2rfJX|PzMWhceS-bb3SvrsPo"`dbp~lV}sT!lZbCd]E>]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|`G}OS@mC)SwkfT`QY]eL?^z HSF{,ComMOx8yT,I)_|_n?_;?oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAh.iOWhbk1^wK[*oCf!`LY&'!"lC+VCi8T6J3{.4MM1\QUNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? sG9J31S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0