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                            !         !!                )               $        !                $         ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 923323ea198647b2acf073d1819ee4b2Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well$K1363_G_4_Helianthemum_coulteri_trnhKB_3730_POP7_BDTv3.mobSeqPop7z*S{<n1+Pn  g % U 6 ~"%n( *-h0$258;L=@BEGHJ=KGKLKKVJ|IXGFCAw>;852/y,b)X&c# GJ( b l ;<~Ya  8 +}r~  q / ' \ ~ y S  D rIY| /X9( wl`N4,>[WPuz ? ~ # [ ! 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DwX- -Kdqo_F+  "/77. 2Rn|yeD"5eV'DxS% Bmf= &D\ihY@& "$" !/880"(D]jiX=9\v{fH* ,BU`aXF0 '+( 7LY[Q=$ 4^U'AJ1mH5UltmZB+     ,584) ,EZfg[F-/QqiG' 4Wza?$2QsdE,"4QrsQ0,E[jmcR=+ "*131) ")+)# )8BE?0  4Rl{zkO.3[{U- #Ir\5 %Cc|sX;".EZhkcQ9" .CSXSD0 $**# (9GMLD7' #;Vkto\A%$Cc|rV6 .EYb`S?)      +7=<3%&AXdaQ9 ":Rdoqi[H3 &9KVWN<' &(#"4DLLD6&$7MamneR:#3TuaA'&;SgsthT;%!4J[dbVE3$'9Oev~xgO6%".FeuY>,(2F`vzeM9./:IYbd\O?1)(/>QevtbL8,*5Ibx}hP:-+2@P^ghcXK>646<CFD<0$$5J`r|{nX>&0Ug=#2WuP95Dc_@,$(4BMTTMA2#                     !$%$!$')(&$" !!!! !%'&"                                                    lb@wAHAPAKB.bcpKB_3730_POP7_BDTv3.mobtermAAGTGACGCTTTGTCCATATGTCGTATTGGCTCATCCGCCCCCTTTAAGATAGTATCTAGTATACTACTATACCTTTAAATCTGATATTATACCTTTCAATATGATATCATACCTTTGAATATGGTATTATACCTTTTAGCCCTTTCATCCGTTTTTTTTTTTTAATTTTGAAGGATTAAAATATAAAAACTCATCTAAATTATAAATAAAGAACTACTCAATGAAGGAACAATACCAAAACTCTTGATAGAACAAGAAATTAGATATTGCTCCTTTATTCTTTTTTTTTTTTTTTTTTTTTTAAAAAACCTAAAAACCCAAAAAAAAAATTTTTTTCCTTTTTGAAAGGGGGTTTCAAAAAACCCTGGGGTTAGGGGGGATTTTTGAAATTTTCTTTTTGGCAAAAAAAAAAAGTGACGCTTTGTCCATATGTCGTATTGGCTCATCCGCCCCCTTTAAGATAGTATCTAGTATACTACTATACCTTTAAATCTGATATTATACCTTTCAATATGATATCATACCTTTGAATATGGTATTATACCTTTTAGCCCTTTCATCCGTTTTTTTTTTTTAATTTTGAAGGATTAAAATATAAAAACTCATCTAAATTATAAATAAAGAACTACTCAATGAAGGAACAATACCAAAACTCTTGATAGAACAAGAAATTAGATATTGCTCCTTTATTCTTTTTTTTTTTTTTTTTTTTTTAAAAAACCTAAAAACCCAAAAAAAAAATTTTTTTCCTTTTTGAAAGGGGGTTTCAAAAAACCCTGGGGTTAGGGGGGATTTTTGAAATTTTCTTTTTGGCAAAAAAAAA>Umv 4?MT^t~!-5>HXery *4>LUboz!.9CN[eq{ "-9EO\gs)6@LWco}&2>JXeq} #/<GS`mw    ( 5 @ M Y g q }  $ 0 ; H S ^ l x    * 5 B M Y e q |   # / < H S ^ j u    * 6 B M Y f r ~  #/;FR[gs~ %1>JVbmx #/<HT`lx!-9ER^jv+6BO[gs %1=IVbo>Umv 4?MT^t~!-5>HXery *4>LUboz!.9CN[eq{ "-9EO\gs)6@LWco}&2>JXeq} #/<GS`mw    ( 5 @ M Y g q }  $ 0 ; H S ^ l x    * 5 B M Y e q |   # / < H S ^ j u    * 6 B M Y f r ~  #/;FR[gs~ %1>JVbmx #/<HT`lx!-9ER^jv+6BO[gs %1=IVbo   $>#%%6>;+;,-;>';;1>3861;>;;;>>>>>;>>>>>>668  +&.>>>;>>>;;>>>>>>6;;>>>>>>>>"611!6.6>>>>>>;>>;;8;;>>>>>>>>6>>>>>;;>;;>;.8;;;;8;;;;>>3.22.8;1;;;;;;;222.22+(&0+333.3.+8>;;3>;;;;82;;6>>8213333>.%3+0.++#'3.2;326$0;8>32;;888;;8>;>>>>>>>>>>>>>>>>;2--&"                       $>#%%6>;+;,-;>';;1>3861;>;;;>>>>>;>>>>>>668  +&.>>>;>>>;;>>>>>>6;;>>>>>>>>"611!6.6>>>>>>;>>;;8;;>>>>>>>>6>>>>>;;>;;>;.8;;;;8;;;;>>3.22.8;1;;;;;;;222.22+(&0+333.3.+8>;;3>;;;;82;;6>>8213333>.%3+0.++#'3.2;326$0;8>32;;888;;8>;>>>>>>>>>>>>>>>>;2--&"                     ?Y:0D 4"#_'4KGD,(PCS[.G_52 3[N#9-Y:YT:BKJ?115^_ V)9[b'@A14B JZ2=N`a8 P'Fa 8Y3>$=V*($[,"L!$4Y@D P G2V`\=U9<*R6*1BB51-&"#@_<b!b+23",+ L@V8@6GHZ!1+ L<48YUF a+<\C6 X"/2<:R@& FLJE*> .>U15(XAOCH/\N:4T'+U,"b<K [RRI'>`W QY)1(`A']H/W=.!W[L8\QK2AZT-4T@N.=)5RF8UT\:+c$-`L&!c3Ed(I97;?%XXQ-9X&IHE=115E(<O&2GUbR)'#%2;&!/OYC/%+X%MB> L mg F^h72! B p)7{g,  +9T@dWX-l*+ }$z/Foqj a!txHfjnvt *2 Lsqx:M#FdA~n,)D6c6eu;)TXj~zrd}."_f(52V ?WQP`0^Xx1v JP}r?D=-6> d=P3kLR?!8}.Gsr)fy'c(( c5e2s5f g @&vW Sn;|skFX] [ l6u Wf( <VB$nCB@A8\xTyj^X7aB>D\68M&U5/('%3!, ;9(. :@  "  x@{pY]dp%}pp#={dp[7Jp]Ne.Dddq0TSR@RHnqeTdqM1,g&brJ7?2wi( t6d$.L#A}8ydVJY(^8Ut,%rIHocX0yo',xnIC.9" _k:x\E+1c?*5 gdkW)*Cu~K!Igx]86TuI;LigENd^]8lsQ / v&uOcvlqg;zy-&f$Y8O@md#+Dc_igVv$_4EnW0a&- & ;94*!%8Q[n gx=#)%G +.mF>w}1%1#$ A0%m  !;$QJ >f=`.{kM>'=kq( _U@`{V+%^?QS8|t*F >8`^=8VWm?mXgD>L :O CWBtpWP9F1%?u*#A6^l.9N>Gqa9&  ,<Sc/WCk~; a6qd e_c?z5};d p5ON8`XiFWzq~ Z"~eTf<d Sf$GGAGCGGCGCCGTGAGGGCCAAAACCAGTTTAACATGCTTAGGACATGATATAATAGATAATACAATAATACATCGATGGAGGCGCCCCCCCAAAAGGCGCCGCGGGCACCCGTCCCAGGAGAAAGGCCCCCTAAAAAGCGGTCCATTAATCGGAGCCAAAAAATTAACGAGGAAGGATGGTATATTGCCTCTTCTCTTTAATACCCCCCGAGCAACTCTCTGAGGAAGCACTACAACCCCTCTCGAGAGAGGACGAGGTAATAGATAGCGCTTCCATACTCCAGACCACAAACGGACGGGCCCCGGCCTTCTTTTCAAATTGGCCCCCGGCCCAACTTCAGGGTGGGAAAACCCCTCTTCCCAGGGTTTTCAGAAAAAAGGGGGGAGGTAACCTCCCCCTTGTTCCCCGGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:55:58 -04:00AEPt3AEPt3APFN APXV2APrN gAPrV1APrX}}pARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTSCBufT"D9CTID CTNMCTOw)CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DnDATA"DDATA"DDATA"D:DATA 'OrDATA 'Or54DATA 'OrDATA 'OrDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ªEVNT ¶EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BYLIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOIS#OfSc"P1AM83P1RL80P1WD87P2AM8=P2BA@P2RL8:MPBAS%aPBAS#PCON/ PCON-mPDMFPDMF#PLOC8*5PLOC8&PSZE`PTYP1PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%#SCAN3SMED SMLtSMPL%%}SPACAmbSPAC#SPACAmbSVER4.0SVER CSVER,,~Satd"^Scal@Scan3TUBEG4Tmpr<UserphARphCH#phDYbigphQLcphTRphTR$7This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN gAPrV1APrX}}pARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTSCBufT"D9CTID CTNMCTOw)CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DnDATA"DDATA"DDATA"D:DATA 'OrDATA 'Or54DATA 'OrDATA 'OrDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ªEVNT ¶EVNTEVNTFTabR=FVocR,FWO_GATCFeat//QGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BYLIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOIS#OfSc"P1AM83P1RL80P1WD87P2AM8=P2BA@P2RL8:MPBAS%aPBAS#PCON/ PCON-mPDMFPDMF#PLOC8*5PLOC8&PSZE`PTYP1PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%#SCAN3SMED SMLtSMPL%%}SPACAmbSPAC#SPACAmbSVER4.0SVER CSVER,,~Satd"^Scal@Scan3TUBEG4Tmpr<UserphARphCH#phDYbigphQLcphTRphTRc( !j r[Z66YM'KvT+F4:H[);@#PA+eg[gUGGN*+j:xUIc.jCxO{VZHngM0xW#nZE35ux2]DM6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(Y`N!:X>zt %v1${NW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`ht1A'obU2kR@ef&e}hw@q}%Q`ALIghJ*(k;:|e}OM *R@_>j23T6Y/Xh]'p8Npdo(ktnP_I[Dd@2g|5J_@A[]"p64y^M_9Ui|?Zm9NVs]uCS':X[ilg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!39G,RS_%ssaP8/GC.JuCglxsQlnWH%`WZ\9~#]c~&Y+0D_j4%OxWYTWrD7gc|?:L]+kT#^\"MLD$bWz%I!|spN$t.DkrD5pY(d+{o.jaxJK[Ylid?O>JWT^Fu*t":Ey+D;UTw|]jL@A[]"TM,s"6rg8Z~9}{MPe!}!"l ppj0j!e~8WY!|s&4)DRhGlB5-u97.{M3>>//x3]wVk=.h^n@EK5llmd$XF$~!SNmW.$7KO]eOY ~?p ,AKM|9v[?|$}~?V'i~s^D\we*V&1pvaUGT[Yu%ozO`w?3 .(53qNsoTGYuB^9UKy\rm#ulc*{d&]?#(eaK|`P@R(RlSR5y=Y`gXBY'^n7a;A{%i7#W yZE!e~8WYH|O[nYL.wJiBwH~?p MJM*{B]9[ikI0o6Gzv&1`Zlc*+?_u';fQGKerctp(kA;M4[!f<,hm<7eaAvG9 HtWo0>B|::zWT|O\rwHB*@Y%H_TQFGa^w^8/HAEPt3AEPt3APFN APXV2APrN gAPrV1APrX}}pARTNASPFASPt"ASPt"AUDTbB1Pt!B1Pt!BCTSCBufT"D9CTID CTNMCTOw)CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DnDATA"DDATA"DDATA"D:DATA 'OrDATA 'Or54DATA 'OrDATA 'OrDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ªEVNT ¶EVNTEVNTFTabR=FVocR,FWO_GATCFeat//QGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~BYLIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOIS#OfSc"P1AM83P1RL80P1WD87P2AM8=P2BA@P2RL8:MPBAS%aPBAS#PCON/ PCON-mPDMFPDMF#PLOC8*5PLOC8&PSZE`PTYP1PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%#SCAN3SMED SMLtSMPL%%}SPACAmbSPAC#SPACAmbSVER4.0SVER CSVER,,~Satd"^Scal@Scan3TUBEG4Tmpr<UserphARphCH#phDYbigphQLcphTRphTR