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    &"                                                                        !"#$% !"#$% ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3f00c3f094bb4e359db3510b136728a1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well K1363_E_11_Quercus_sartorii_trnhKB_3730_POP7_BDTv3.mobSeqPop7zY.?B9+$,Ht*50!0Ru 1d R/ r , G]St"'U,t28M? 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LVc+`[1R?ER],08XW/C> 8MVFZ.#9 B>F;OPA[Z*M;/2 `CJVC)FUc#L)&?dS79>6QD+J Q7`V5bT`9 F!d_|:`6. }P`( s9[MG: >/w LC[0aT5 /FGa=gwMGM4st 'Qa$E Aa0" ;$KUyWp  ,'edS8$Qn7a{"N*agZYkZ?M!#G i\|#h w)u.9{/Kp4G_gkDng_B|w\ r~. u]r-b;vN! ?mcfW/;=K'pt{pw(3S58kfj't% i_,1Wr_^)et]aC# %E =NYtBIz=fOxve >Abjy)w6A}+ W'Ov Zn5b^?9~(|3~ HV(1 0 FQ0=d  leN R>xp>sY>mVLZb) 7<?KD*'I1&CO.(_v>TNba7; 8S m o>scQ(0' hV^) )1 ), ;E )4% !(!:     4!-M !D / 9    LD -; } 0<2 '(J2f"$K 41*$?f,3A3P`VQE5!+18`(#9G .A&KyGA6=b:Hp/(fRHFNXH^' p&D-pOI8q6lMxe9v9K8@p281@p=8}lq~%n%%XrUb&o"8L;"^8R]5s.8RjA7;/8S%dY)-$~!ATd&q^p0 },M3@[Qp4-G7W _Fi6 ` ,#p@)z hzZB_p#hQ$PD,y"6,P+z@mL>|s4pIh8ulo~D#!>"e )<',s9BK@Z 7.1nqK* <3Hub{6 S6PL+>sguY~p)66I@F$GKO9u537ev &[C`ecsD\1{L_G_FO"Jxrm6   Z^y#=-1u2(/ ?9`DJW1i5;IU^Ge[>0V)N@TG6C9%;JJ&%Lo(G&P"fL#bCjJY=@r$*|0JkRn/RS!gBQcB98GKCHI:W;hnANiO'I`fFAaqqM"EhC;.6C64d[XNACHE|E'4:\{I0iqd`^5RyenjxW];-V],"3,?THUkPVIlRS)~\R=O/"@H+^0TG@VO{6@Z[1E@+<:6VF! k!`:;[<lU;LS%8,rSvkE*#+j$0BDAS{EjXMHuV@6BK6]H0o1Uv+<WxWzWwnQ:Y4M87E@?B14dSD9%uOUh|Nrt spSM|Wt\T3}`uflij3^o}8d0\b(% Dgd#1CCCGAGAGGCCCGCCGTGGGACAACTATATTTACATGCGGGAATCTTATACACAAGGTGTATAGAGTAGGGCACACCCGTAGGACCCCCCCCAGAGGGGGCCAACGATGGTAGAGGGGTGAGGGACCCTCCCCCGGGGGGGGTCACGATATGGTTTCGATGGATTGGGTGCGACATCAACGAGAAATGGAGGGGTGTATCGGTACCTCTAAGAGGGGTTTGTGCGTTCTGGGGGGGGGGCTGCCCCTGGGGGGGTTGGTTCACCCCTTAAGAGTAGCGTGAAGTGATGTTGGGTGCGCAGGCTATCATCCGGGCCGGGGAGGGTTCCTATGGCCGTCCTGGCCGCTCTAGCGGTAATTCTCATATCGGGTTAATGCGCAGAGGGTGTATGCGCTAAGCGCCCCGATCTGCCTGGTGGAATCACGACAATGGAGACAAATCTTCAATCATGGGGGGAGAGGATGACAGAGGGACACCCCCGAATGTGGGGGGGACAAAAAGAGCCCTCGGCTTTTGAGCACTGGAGGAGTCTAAGGGGATAATATGAGCATAATGGTGCAGCCACCAGCCGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:56:57 -04:00AEPt3AEPt3APFN APXV2APrN ?APrV1APrX}}HARTNASPFASPt)ASPt)B1Pt(B1Pt(BCTSI.BufT"DCTIDCTNMCTOwCTTL  CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DFDATA"DDATA"DDATA"DDATA &MDATA &M3nDATA &MFDATA &MDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ‚EVNT ŽEVNTšEVNT­FWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~GLIMS!!LNTD2LsrPaMCHNMODF 6MODL3730NAVGNLNE`NOISOfSctP1AM<x3P1RL<x/P1WD<x8P2AM<x@P2BA<<EiP2RL<xRbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`_dEKW\lWQ7M31pfQ>?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3W*c3oAa!jv3}H1,Cz#Z|#e1a_WTDr]?'Hrz9Drv4~K3qoI>*?ag5d7m``k:!.tk}Y=52r =&e=.7I 9'kT'_C:j|(5,grOW1 *>khd|$HRNkg4K0T3L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@Cz6AqdIqN6`aD/,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9]CR"lWW .C&q6X+9muy%lFLuLXtr:E6=W{Z=z&9 J{M!E7hVo+&k=;A{%i|o(>V&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBAroW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#