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(%!("   + ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bcaf69d00934454d8866d1dc11c86d0dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well$K1363_D_7_Valeriana_candolleana_trnhKB_3730_POP7_BDTv3.mobSeqPop7z +>^Eu)u87h Y !q#(G-Z3 9\@EGOXakv>~8sjc#\XTRRHSUXH\ag$lrw|zsl\d]PUNGA^;V50,)'l&F&&o'U()+-/14I68:J;}<<&;:8742/+(\$!Id]G H .  p _lI~Q  H u g 1   V  8 P m r {   /(2L=Kbt>bWPJrC=8D42D0.,*'H$"!,h.zQ]J<Q!s#%k'(@))v)~)5('&%"x # &#& "vQ<b):t<1 l z   R jDp-~gXSV[YG!Z $33% Iz~o\B#rP9/067,k6 #;Qcqxytkb]]ad`S;",?Si(6;7*x`TOH7  {\: &Gid>$ (D]ljY>" $A\pyxsqxoI(  )-,% !  &>S`bZM<+  "1:?AEQe|~dJ5(       !$$     (,)"$$!  !,5=HZulSB;:<<80%  #Elm8  1BMOH:+#&4Lf}")4BR`m}:Wegeekv 0M\XAyN0! 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(13,$14-   %&&%&&&$     %,+$    #                     !$                                           "'&                                 -78/        !  +20&       "#           $&!                      $)("                #*+$   $!                        %%    "%#                 &)$                    !&$        !        %%                     #,-'  "'$               '285-#  #($    !$      "" A1'A!A\;A KB.bcpKB_3730_POP7_BDTv3.mobtermAGTAAAGTGCTGGTGTGGATGTATCATAAAGGGAGAGCAGATTCTTTATTTGATACATCTTTTTGAATGGTTTTTTAAAAAGAGGTATTGGTAGGTAATCTTTTCATTTTCCCTCTCTAAAGAAGAAAAAAGGAGAAATAAAGCCCCCTTGCTAGACCAAGGGGGGATTTTTTTTACTTTTTTTTTTTTTCCCAAATAATATACACTTAGAATAGGTTTTATCCGATTTTTGGTTGGGGTGGGGAAGAGAGGTAAGTTTTTGGCTGAAATTTTGTGGATTTTCTTTCTACATAGCCAAAAGTAAAGTGCTGGTGTGGATGTATCATAAAGGGAGAGCAGATTCTTTATTTGATACATCTTTTTGAATGGTTTTTTAAAAAGAGGTATTGGTAGGTAATCTTTTCATTTTCCCTCTCTAAAGAAGAAAAAAGGAGAAATAAAGCCCCCTTGCTAGACCAAGGGGGGATTTTTTTTACTTTTTTTTTTTTTCCCAAATAATATACACTTAGAATAGGTTTTATCCGATTTTTGGTTGGGGTGGGGAAGAGAGGTAAGTTTTTGGCTGAAATTTTGTGGATTTTCTTTCTACATAGCCAAA BVr!-CLTd !0@LYr )=Sgw %0<HRZdmr{5@J]jv )4<CQ]jt )5?JVcoz *6DO[is  $ 0 ; F R ^ i u  # - 7 E R ^ h u   ' 3 > I U d n ~    ) 2 : E P \ j r    ) 4 ? 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O n? kFcc"(3?Z>4$ 8  J4zdL V^#y 5OSK8jP)M9y@ lIN/3FE{Y[!Zw a^ 1x  Qti$1F &&k=3  3|>o{_1a ;`52*0&H'@XX88psh8mhth&HI|9W(R]RF @J NJ'u&|IH. 09Q#wy(V871P5RZ8'2'0^9#ZeJA+ mp)8(I Vpa>u+-p pp'd? lvZy;+ p wL}l[4sII8DH: ; d# jHq*!p{ 0ih1p>CR@nfQd3[UT9?~p81Cp dm-Oa$R_|_|/25\ <I 44q0g1+/>8$IJw.JE$62  i^l 5ANh F%01j#oX)FBlR^ZJ3F<p=8h~okqQ}?5U<v!-#6O Q3%JO3Qnh+w r7B~ B<"~_,a 2j::%  GTGGCCCCCAGACCAAACCATATAATATCGAAACCGAAGTCCCTAAATAAAATATTTCTAAAGAAGGATCCCCCAATGGGGAGAACTCCTTGCATACTAGCAAGGCAAAGGATCTCTCTTGAGGAGGGGTTTTGAGGTATTGATTGGTCATGCTAGAACTAAATTAGAAGGGACATACGAGAGCCGACCCTTACTCATGATATACAAATAGGATATGCCCTATACTGAGGATAGGTAATGTTAAGCAGCCTCATTCCCAAAAAGGTGTGGGAATAACCACGCGACCACTTCATAAACGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:56:31 -04:00AEPt3AEPt3APFN !APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSS7BufT"DݡCTIDsCTNMyCTOwݑCTTL ÜCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D^DATA"DDATA *UF*DATA *UF;pDATA *UFDATA *UFDCHTDSamDySN }Dye#DyeNÈDyeNÍDyeNÒDyeN×DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FWO_GATCGTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~QLIMS!!åLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS;JOfSc/P1AM+VG+P1RL+VDP1WD+VIP2AM+VN-P2BA++PP2RL+VKPBAS++<PBAS++;}PCON++CPCON++BPDMF PDMF;aPLOC+V@)PLOC+V=PSZE`PTYPݙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate PRunN""IS/N%;BSCAN3SMED SMLtSMPL%%SPACMSPAC;ZSPACMSVER4.0SVER S,SVER,,Satd/^Scal@Scan3TUBED7Tmpr<UserphARphCH;xphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN !APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSS7BufT"DݡCTIDsCTNMyCTOwݑCTTL ÜCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D^DATA"DDATA *UF*DATA *UF;pDATA *UFDATA *UFDCHTDSamDySN }Dye#DyeNÈDyeNÍDyeNÒDyeN×DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FTabaFVocaFWO_GATCFeat..aRGTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANE9LAST~~QLIMS!!åLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS;JOfSc/P1AM+VG+P1RL+VDP1WD+VIP2AM+VN-P2BA++PP2RL+VKPBAS++<PBAS++;}PCON++CPCON++BPDMF PDMF;aPLOC+V@)PLOC+V=PSZE`PTYPݙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate PRunN""IS/N%;BSCAN3SMED SMLtSMPL%%SPACMSPAC;ZSPACMSVER4.0SVER S,SVER,,Satd/^Scal@Scan3TUBED7Tmpr<UserphARphCH;xphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23isFb$t\c+6e=J#=0\ED3oB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZG>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H%R74@nB\[Q!(NnLTv`tp?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6[OCy KJFU'.QHT]R06`NV%]?EZX/-k'iJ4~CubWk@X=<-8 )T;Fi$t_SF`|zo;LY&PE!am&,*oe?F[Z*`E }Z&[g;z%!SNmW.$7KO],n`!3o %>