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GCK>H6%  !+1HTTB.;5'   "((3A3=+# $-"%9!  6"&N, "&(-&#FHM43?G:5+;H65.RD4CB_[GcTm[crYyYbqj|Wvww}tqafg^\:^_fW`KD2(:#)'/::87+"&(0&&   ()!B=-     $ '!  <   & $$   #            "          (         !         *&                                                                                                                                                     ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6cac2195eda74ed8acffbc6dba858649Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well$K1363_C_5_Castilleja_tenuiflora_trnhKB_3730_POP7_BDTv3.mobSeqPop7zUT"yi 1 ) = _ u@8n"g%Y([+h.158K#2P|JDh'EH<.-;Qgy\%.JR?%P^;c3}ps|km|mWLLV`eaVF6( @2}Qe0 $-.'  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'-11/*$       %/30( #'+.12.% &6BD>2% ,<HKG>1# +?OVP?(   &,/.-,*&  '-0232/+$     +5<<7-"  )3>EGB8)      $%   #*/00-)")6?DC=71.*%          "(+,*$       "(****,/0-%     &,/258=AB?81-0;M_lpi[J=658;;81*# !%+04555553.&#*,*%""&+00,#  ",47888;=>:1%               "$#   %/661(          ",0-&!%%        %))'#                                                                                       "      %%                                                                                         !((!        )*"                                                                                                           ePAOAMjA\\AAKB.bcpKB_3730_POP7_BDTv3.mobtermAAGATAATATGTTTTTGCATTCGCTGAGCTACGTTTGACCCCTCTATGATTACTATTAAATCATCTATTAAGAATTAATATTTTAAAAATATATAGAGGTGTTCAATCATTTCATTACGATTTTTATATAAAAAATGAGCAAATATCTTCTTTCTGTCTGAGATATTCTTTTCTTAAAAAAAAAAGATTAAGAAAAAAATTGTCAAAACCCCCCAAAAATTTTGACAATAAATTGTATAAATAAAAAAAATTTACAGAACAAAAGAATAATACTCGATTGTTATTCAAGATAATATGTTTTTGCATTCGCTGAGCTACGTTTGACCCCTCTATGATTACTATTAAATCATCTATTAAGAATTAATATTTTAAAAATATATAGAGGTGTTCAATCATTTCATTACGATTTTTATATAAAAAATGAGCAAATATCTTCTTTCTGTCTGAGATATTCTTTTCTTAAAAAAAAAAGATTAAGAAAAAAATTGTCAAAACCCCCCAAAAATTTTGACAATAAATTGTATAAATAAAAAAAATTTACAGAACAAAAGAATAATACTCGATTGTTATTC1<HP_fqy0DUkw~  '7@MV_iv'4AKVcnx"-7DMRY^dnr~ *4AKWcp{&3BLWbly ".:FS^ju $/:HR\gt   # 1 < H S ` k u    * 7 A L W d o z   * 7 A L Y e p }  " + 7 F P \ g v 1<HP_fqy0DUkw~  '7@MV_iv'4AKVcnx"-7DMRY^dnr~ *4AKWcp{&3BLWbly ".:FS^ju $/:HR\gt   # 1 < H S ` k u    * 7 A L W d o z   * 7 A L Y e p }  " + 7 F P \ g v    "''  $ "(*( *)(""!    3'8)2 ++" ;;;+-    +$&3+"2"  ";1;$&6"""   $'+0$  !.    "1,''   "%             "''  $ "(*( *)(""!    3'8)2 ++" ;;;+-    +$&3+"2"  ";1;$&6"""   $'+0$  !.    "1,''   "%          @XNd)R[5A'OZT W"CH_1Z3? ` ?Y9,X!,6X]<T(F?^_a0EZ1(ZWb: R 0L ^!\'^0LZKE%QU>AbK ZV C#+E S\,Y?"d(7)_a[[ =cb`)=DKR!4Z #\a5 *LXD;]=a'3K<_R>O5cQ.EAU;P)=J[ ,Q'=\242cQ2Yab6?d9KS'='=S8DH2%#]@:W'= 92Y`2 ]'=xy $+LE )G V5'x#q,|#$9Q. [>me6xF^N -WFi,eK?(<pL,gO^! f '8Z$C~)2/4 C  /<f` OPMELQ of)/)Jj+cH| -.mcv=' HknYtAzRYQqP)f ,HBxfp . a  < 5BT O4B `n<C2`~Hh;bOr~<ZBHw Wg>r6s f.#@';C.)= 4   X$ 2K 21H ' pb(&pppQANppY8*4@:"<~_?'[V=G2R\{X=20C,h + 2 7*Ax A1,X `}2np~'4ng'zpw RtDnB4!<= %S l c_ "-:S%3maL3Zw2_ t4Cqx)* : |egqitn\mtMN$"N (NXfRuC`W<ynj~]apsK`Tp`sL N."ZiG,@EX623]) H tcJ93& #\'`(\]C|GGAGGGGGGGCCCCGGTGGGCAAAATTTAGGAAAAAATAGGTCTATATTAACAATAATGGAACATATAATGAGTAATGGTAGGCCCCCCCTATAGAGAAGTGGGCGGACGGAGGGACAACGAGGAGGGAGGCCCCCAGAAGGGGGAGGATGAATAACAATGATATACTCCACTGCTGTGCGGGGTCCCGCGTGGGCTGGAGTAGCGGCGTTTGGGGCCCAGGGTTTGGCTCGGGAGGATGGCGTGCTGGTGGAGGGTGGAGGGGAGGATGATTGGATGAAAAGAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:56:18 -04:00AEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSWXBufT"DYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DDATA"DZDATA +oVDATA +oV;DATA +oVDATA +oV|DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE+LAST~~ULIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS@bOfScvP1AM<KP1RL<IP1WD<MP2AM<RuP2BATP2RL<P9PBASAPBAS@PCONHgPCONGIPDMFPDMF@yPLOC<E PLOC<BPSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%@ZSCAN3SMED SMLtSMPL%%SPACMSPAC@rSPACMSVER4.0SVER WMSVER,,Satd^Scal@Scan3TUBEC5Tmpr<UserphARphCH@phDYbigphQLcphTRphTR5This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ‡APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSWXBufT"DYCTID+CTNM1CTOwICTTL TCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DDATA"DZDATA +oVDATA +oV;DATA +oVDATA +oV|DCHTDSamDySN 5Dye#DyeN@DyeNEDyeNJDyeNODyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabeFVoceFWO_GATCFeat..esGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE+LAST~~ULIMS!!]LNTD2LsrPaMCHNMODF ~MODL3730NAVGNLNE`NOIS@bOfScvP1AM<KP1RL<IP1WD<MP2AM<RuP2BATP2RL<P9PBASAPBAS@PCONHgPCONGIPDMFPDMF@yPLOC<E PLOC<BPSZE`PTYPQPXLBRGNm#RMXV1RMdN  RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate RunN""S/N%@ZSCAN3SMED SMLtSMPL%%SPACMSPAC@rSPACMSVER4.0SVER WMSVER,,Satd^Scal@Scan3TUBEC5Tmpr<UserphARphCH@phDYbigphQLcphTRphTR"3Z2VW)$>ZHm? 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