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                  !/% &!$=*&#"$!?30-3 3! %&      !   .+..&31607!         *?+3A0(2>    +)$.>34S1:%)&        &,  -;?>:;@>1<".8&($# !         )J9UUZFEDYA4>&.$*22 5( *   ))                                                                                                                                  !                                                                                            $                      &           %                                    #    "                                                     $       "                                                    #                $    "    &        ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e2e123c065e74721959f47b97648a34aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well%K1363_C_2_Polypodium_plesiosorum_trnhKB_3730_POP7_BDTv3.mobSeqPop7z$3Ns +Hbx 3Vt9[v%U' M cr(!$(+e.26y:c>8AECHeKGMPOR`TU$UUwTS+Q)NKHE(Aw=951-)&f# <t@6 3 !!"%"<"$!!' ).NH4(4d7 y & r : /  ^ y zJ-~GyWC;<?BEHLR[dlomcT?(6Z*    P / $ } 2 a }l@m '/:oG UevoT^JNA|6:-% ?z9_ }  nWzXB4%o&alqcXG*Rvrz7PYP4<W~> %& "=_yYA2,0ChH4ay~rT'zP9:QwczP<5/"   5K`oxV#hH0"!/Oz54+|_K=3,(&# !+5:92)! 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""+1"+#2-"' ' "1    - a6=60T?0*D 'K@QFO:@ )D-^Q'WS<R6Y8H*5aJ0Ka97/')a^GD0WQ[2K*+=?* %!X8Za"#R<W78 E%N +U5KCKR"T\$_+\.!2" HN%9 caQMHOW@c>4S 4!TR=KHI+NAHIJ:G1Mc>093YB)= K0=B+ @9> /ZT<?G/K%6< /aY,A\' BaE/W7O] %.[2>:="a-BEF:2\ 5&c9Kb7QK/RWL@BC\_@; Yd>(,9`5(74Za80S ,,K1&H,IQ:S1NJKK1a1XWM;KYVI:c$&< 9G\&&5':O]& AQG 1 BPYJ9SLS7F^ >&?V<I9A1M6aQ":YY6?6 G ;7TLA8^2D9a( ECT)5O/UL*A- L DPN9 ?O 'ZO4[OV+<^D,8*.EHO*+UGI)EAb2%5TM',+56.<RYj H2\ "+)21C r 8mj ["  :v+NXq"nBD|RM/y99e Q`=yIvN. 8Sa5-SITV) _A!@O@_p#W2.vn)H)~Zf|/(~s.hYhXe4=2glUuefG'&w:Re~1:4d4Zc:[Lgf[Row1Xt<~}O34kb^E*!)O$b~39vxj8xs)lNB/AEjC&qKE*!]'|4)[Eqbt&6+XgPqY_9y^(#eYF4 }*s[],VyDwFcWNf EE M4. }i|7H9Z=a[18&~]VPUl=>IJRR<exh<!$ W-!  $.4QN@,  =$@ZhL<@7&B)9  R.6Q  T=/ -&      :3 ( :jU/?+   :?R.@!:M9R_- ,110-B(S &M 3'  C'J?=7R1!&)PCTK/S("u y\La L7~qhyUp#},lX|{0D\3$d-m ^XQ 8mAvpm88B6]|?zABC dAJGo8SI o{}u  0%@jv#,dE5Oijk /Py9$V1>.$#');'&F^2b%t^+yfXwtS^d Zwnm  79.1OSEh='2 d< dx k $RN,LS ^,L2[@$0) !w  \ ! |sD $ 5 oT0.:Dx#um1`<U\vj~x9,[hv/a?lq 9J=uaA0yLvB'po|hsqw }Vv]QHb,'Eb?WfbWNVZT,KcmHp$`>{^KTX5,tq|8Q\[dpc"[a$Fi4KWCGy9c`b:[^M}?X||/mF5 D?}Z5T7aAUHqs|;O=_m=Ms^Z7c#I~AXEy>4@gz~OaWjPvdQrJ]M[_XNKNQKKEWk&L^6oP\On[fFBO}gGE_yoJkwrQoNGOl V\UwVYaJ=ZE`yO\widRMs?=t9I&[.=ty ytYM/pMts?.@"/\^lPgqS} TZm=)KAGGTGCGGCGCCGGCGCCCAACCAATTGATAAAGCGAGTCTGTGGATACATAGCACAGGGGGGGCACACGAGCATGATAGCCCCCAACGAAGGCGGCAGGGATCTGGCTCCGACGGGGGGCCTTCCCAAGGAAAGGCCCTCCGAGGGGAGGGGGTTGGGGGTCGATGGGGCGAAGGGAGGGGTGCGGGGAAAGTGGTGCTCCCCAGAAAAAAGTATGAGCCCGGGGGGCGAGAGGGGCCTAGGGGAAGGTGGCTAGCTCTGCCCTCGGTCGTAGGTCGGTCCTGGGGCTTGCCCTGTTGAAGGGTTGGGGCCGGTGCTCCTGTCGCTCTGGTGAGGAGACTACAACACGTAGGTTGCAGGCGTAGGGAGGGTGCTCATGTGGCTTGTGTTTCCAAGATTGACTAAGGAGATACATTGTATTTAGGTCTGGCAGGTTTTTCTTTAAGAGGGGTGTGGGTGTTTTAACCGCCTTTTTTTGTGGGGAGAGTAAATTGGGTCCCTCAGGGCTTTACTTAAATACTTAGGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:55:52 -04:00AEPt3AEPt3APFN "APXV2APrN APrV1APrX}}ARTNASPFASPt&ASPt&B1Pt%B1Pt%BCTSX7BufT"DܡCTIDsCTNMyCTOwܑCTTL œCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D^DATA"DDATA )CR+DATA )CR7DATA )CR7DATA )CRܽDCHTDSamDySN }Dye#DyeNˆDyeNDyeN’DyeN—DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FWO_GATCGTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~VLIMS!!¥LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS/KOfSc<P1AM DP1RL ?P1WD H>P2AM P~P2BATP2RL L^PBAS1PBAS/~PCON=PCON;PDMF PDMF/bPLOC 7PLOC 3PSZE`PTYPܙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate PRunN""IS/N%/CSCAN3SMED SMLtSMPL&&SPACAk-SPAC/[SPACAk-SVER4.0SVER X,SVER,,Satd<^Scal@Scan3TUBEC2Tmpr<UserphARphCH/yphDYbigphQLcphTRphTR(ҁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN "APXV2APrN APrV1APrX}}ARTNASPFASPt&ASPt&B1Pt%B1Pt%BCTSX7BufT"DܡCTIDsCTNMyCTOwܑCTTL œCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D^DATA"DDATA )CR+DATA )CR7DATA )CR7DATA )CRܽDCHTDSamDySN }Dye#DyeNˆDyeNDyeN’DyeN—DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FTabfFVocfFWO_GATCFeat//fRGTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~VLIMS!!¥LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS/KOfSc<P1AM DP1RL ?P1WD H>P2AM P~P2BATP2RL L^PBAS1PBAS/~PCON=PCON;PDMF PDMF/bPLOC 7PLOC 3PSZE`PTYPܙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate PRunN""IS/N%/CSCAN3SMED SMLtSMPL&&SPACAk-SPAC/[SPACAk-SVER4.0SVER X,SVER,,Satd<^Scal@Scan3TUBEC2Tmpr<UserphARphCH/yphDYbigphQLcphTRphTR-Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&rYA z3]#gi8$7oA8V/5=q:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]4f+nb<,`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pMCWk>6UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQno X:QJf9/qwAxew>6?|$lQ%:XGTtrWV*AEPt3AEPt3APFN "APXV2APrN APrV1APrX}}ARTNASPFASPt&ASPt&AUDTveB1Pt%B1Pt%BCTSX7BufT"DܡCTIDsCTNMyCTOwܑCTTL œCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"D^DATA"DDATA )CR+DATA )CR7DATA )CR7DATA )CRܽDCHTDSamDySN }Dye#DyeNˆDyeNDyeN’DyeN—DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FTabfFVocfFWO_GATCFeat//fRGTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~VLIMS!!¥LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS/KOfSc<P1AM DP1RL ?P1WD H>P2AM P~P2BATP2RL L^PBAS1PBAS/~PCON=PCON;PDMF PDMF/bPLOC 7PLOC 3PSZE`PTYPܙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate PRunN""IS/N%/CSCAN3SMED SMLtSMPL&&SPACAk-SPAC/[SPACAk-SVER4.0SVER X,SVER,,Satd<^Scal@Scan3TUBEC2Tmpr<UserphARphCH/yphDYbigphQLcphTRphTR