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"             +5?SNJK;'%    $?ASsMn_dS0)/!:?WWvkb^pP=9       #+bovnu\AD&   ;:UTe`;30)        )B5UDGJ=."  ?@XF`LM1/ "             +NNf~gi\G#7%    $=RHVRSG7!,)2./IJ[L\dd4U.(    $.L]OaZG\+/  .3AZh`_aKG<8 -&2:LI[clN8>., (        +>P6K?H;'    0GINRMMR=,(  86;H=JU6*$   '&>IW_DV<>*$    B'0I;D6&$  #2T9U@E>/1'    $#1M;WcIH;?%   , *3HScO5?*?!      #<SDQ@I8)  6AGM]meVP@+(    "-Asd_zmuGTB0,,'"!0*<5+""                                                   "  ( %  #  !                                                                                                                      ,,,IIT^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRm~}{xwurqplLPPHHHeee}}}   '('000888@@@HHGOOOWV[^]dddkkkqqqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 47239a3a8a6041b89d363295478a56d7Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-13_17-25_19873130Seq1014810148_Lehman_K1363_trnhjmadera96-Well"K1363_A_9_Prunus_brachybotrya_trnhKB_3730_POP7_BDTv3.mobSeqPop7z8:=CO`wqly=]~*fq#j1  4 E Z;j"&_)-w16H;AHoNTZ`flszsztn\g``XQhJZC=83.*'%%$%&'(*P,.p1U47:";<=3=:=<;:`841J-)&#!NT$chi@  o  , ZcNKLF2 w )G+wt/   - _  "zJp"7H9 #%(09EnSe3yihUF 90)%!2[`t)G$ec _ y rd*  V H$IcSd7m   ;  VfZHXtkf]G#rbbn@B;F'Y)/E\sp9z i@,# }p{ ?e{v`@lS>23>LZbfhjms{nVrpU&FM   !,20(, j _\ ^<q9"$  &,*! 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''          (12)         !'&     %#AOA()Ac'A(KB.bcpKB_3730_POP7_BDTv3.mobtermTCAGTCTGCTTGAATTGGTCTGACTGGCTCTCCGCCCTTGTCTATTACAATATTTACACCATTTATCATTACTTGTAAGATAAAATACAAAATAAACTGAAACTTTTAATATTTTAATTAAATTTTGTAGTAAATTAACTAAAAAATATATAGAACAATATAGTAATATAGTATAGTAAAGTTAAGTAGTAAATAAAAAAAAATAGTAAATAAAAAAAATACTAAATAGTATAGTAAAGGAGCAATACCAAACCTCTTGATATAACAAGAAATTTATTATTGCTCCTTTACTTTCAAGAACTCGTATATACTAAGACCAAAGTCTTATCCATTTATAGATGGAACTTCAACAGCAGCTAGATCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCATCAGTCTGCTTGAATTGGTCTGACTGGCTCTCCGCCCTTGTCTATTACAATATTTACACCATTTATCATTACTTGTAAGATAAAATACAAAATAAACTGAAACTTTTAATATTTTAATTAAATTTTGTAGTAAATTAACTAAAAAATATATAGAACAATATAGTAATATAGTATAGTAAAGTTAAGTAGTAAATAAAAAAAAATAGTAAATAAAAAAAATACTAAATAGTATAGTAAAGGAGCAATACCAAACCTCTTGATATAACAAGAAATTTATTATTGCTCCTTTACTTTCAAGAACTCGTATATACTAAGACCAAAGTCTTATCCATTTATAGATGGAACTTCAACAGCAGCTAGATCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCA 8Sdx$-@GR_es| +7AN]gny".;HR[goz'/;EOYdnz  *5?IVamw)3=HT^ju(3>GT_jv #/9DQ]hs~    * 5 A L V a n y   # 0 : F S ^ h v    % 0 ; G R ^ j s   % / ; G U a m w   , 8 C N Z g s &1=KTcn|'2?LXep|,7ER_ix+6BM[fr%3>LYeq~ 8Sdx$-@GR_es| +7AN]gny".;HR[goz'/;EOYdnz  *5?IVamw)3=HT^ju(3>GT_jv #/9DQ]hs~    * 5 A L V a n y   # 0 : F S ^ h v    % 0 ; G R ^ j s   % / ; G U a m w   , 8 C N Z g s &1=KTcn|'2?LXep|,7ER_ix+6BM[fr%3>LYeq~          +2*6,%,>3 ;$+;;8833.'+&    .+8>682.;>663>>>;>1#0;;>    3'&20&>;>;;;>>>>20;;321;;;208>>>>>1;8.8682.880028>8;12;.8;>;   $#00>828++3;2;;;>>;8;(-8282(;>>&8.##""'#&2.02888;;>;;>>>;;831.8;.0.381;28;(2((08";''&!.1;;.621182"  ".,.808228+82;;;;;;3>;;;;>>;;;;82.22138;'...1;2.#.+28>>;;3..82;;+.8..+;+;8;;,;;;-2           +2*6,%,>3 ;$+;;8833.'+&    .+8>682.;>663>>>;>1#0;;>    3'&20&>;>;;;>>>>20;;321;;;208>>>>>1;8.8682.880028>8;12;.8;>;   $#00>828++3;2;;;>>;8;(-8282(;>>&8.##""'#&2.02888;;>;;>>>;;831.8;.0.381;28;(2((08";''&!.1;;.621182"  ".,.808228+82;;;;;;3>;;;;>>;;;;82.22138;'...1;2.#.+28>>;;3..82;;+.8..+;+;8;;,;;;-2 @D?V[`&4@" )QM;C L a7&_8\KG 55 HZ7L<5 XN=;&"SWCP@<::M-B[S,8P6MC)N9WR"*39B5 (:AP NEG,\+:Y@ ]XcQA3c9/TSTaT,W7L^G2.WD_7S@YT%ST2O7CSA>a[>KOBQ+55U6>A36\4AKM.2HD&=@5-H= @b\73-X XG ^:dUU B9=T[!-W$P*>M\B-QGA=b==D]L?M'/b /LT4N[NX#@aLFA08<]W(*&U W< C N?RKVY);8)=Y7b2bd[_`<*3<UR19>)439U1TGcDC,!)b5A&1I7)AH? <*K=[]G>:"j_/ {0n ~H AWaEj9uaRz:Z9p7 u\F-sN<Z|5*P  I]l '<2>i/_I'7HVDS:}#aXG|m(z3?)6D!.)74MH~N:&r5Uy-+_ZYUC0 :VO . >AB4  L}5q.f*stLl(8 RzWbRS(,4f=7`Ekezg}3!*g!k=._@N-hX?LF[ VJ^Jx=];3N 5%)OQqW]$:.QPXCA I"   )-sV CRi'   <(2?+"%/ X    8 HEC;# &&66$ 96<'D !$3-pIZL~pYx`r8ddAQp]\)d+OWx363KpUMp/   /C+h}64|#vJ)CpF(J5R.8B8_IB;XFjD e~;88$%%e~1j^~4Up ; 0W|~f=8Bp0-P: ;z F6t"{$huUs44 8J <Yz7xn/TQDcyUeEB 3# 2%R 0"nFEiy#+te8X_USuD+UQ6p X `4ZVH`DOI [I2C_CJ8J+fMrkC#0VGF3@MMOO<N4r&PgJLNIcfJ&'D7TVC^<>XJE-OF[Qjaj1o-.8cKY8)?g@STA`99i3:F+RXa2V,~H5b7E[XhgVfP1)IlDwVDMu?fFbpnqhbwC^F_[HV=mxZ~uBFd?;PIOc#7R#-b$W&3RaHTO9GsDGd>#%gAAGTGGCCGCGAGGGCCCAACCCAATATAAATGCGAAGGTGAATAATAGGGTACATACAACCGATAACAAGGCGAGTGCCCCCCTAGAGGGGGGGCGCTGGCGCGAATTATACGGGTAGCCCCCCCAAGAAGGGGCCGGCCGGGGTATATATCGGGCGGGGTTAGGGTCGCGGATAGTCTTGACTAATTGTTGGCCCCCCCCCCGAGGGGCGGGGGGTGGCGCTGTGTCGGAGACCCCCCGCGGGGGGGCGGTTCTCCAGACGCTTCGCGCTCAGTGATGGTTGGTCAAGAAAATCGAGTACGCATAGAGGAGCAGGGGGGAGGGATAAGCGGCGAGATGTTGGTCGAGGACAAGAAATAGGTCGCCATTGGGAATAAGATCGAGTCGCGCAAGTACCAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-13 18:56:50 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSzBufT"DܙCTIDkCTNMqCTOw܉CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA .]Z DATA .]ZBzDATA .]ZDATA .]Z.DCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FWO_GATCGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~xLIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOISZOfSc8P1AM"jmP1RL"gKP1WD"mP2AM"sP2BAvP2RL"pPBAS\TPBASZPCONePCONd)PDMFPDMFZPLOC"aPLOC"]PSZE`PTYPܑPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate HRunN""AS/N%ZSCAN3SMED SMLtSMPL##SPACAmSPACZSPACAmSVER4.0SVER zSVER,,Satd8^Scal@Scan3TUBEA9Tmpr<UserphARphCHZphDYbigphQLcphTRphTR0This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSzBufT"DܙCTIDkCTNMqCTOw܉CTTL ”CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DDATA"DVDATA"DDATA .]Z DATA .]ZBzDATA .]ZDATA .]Z.DCHTDSamDySN uDye#DyeN€DyeN…DyeNŠDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FTabjFVocYFWO_GATCFeat//*GTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANEOLAST~~xLIMS!!LNTD2LsrPaMCHNMODF ¾MODL3730NAVGNLNE`NOISZOfSc8P1AM"jmP1RL"gKP1WD"mP2AM"sP2BAvP2RL"pPBAS\TPBASZPCONePCONd)PDMFPDMFZPLOC"aPLOC"]PSZE`PTYPܑPXLBRGNmcRMXV1RMdN MRMdV1RMdXVRPrN1RPrV1RUND  RUND  RUND  RUND  RUNT (RUNT 7"RUNT 0 RUNT 63Rate HRunN""AS/N%ZSCAN3SMED SMLtSMPL##SPACAmSPACZSPACAmSVER4.0SVER zSVER,,Satd8^Scal@Scan3TUBEA9Tmpr<UserphARphCHZphDYbigphQLcphTRphTRrIvMa3o';E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(`"]drh(>LO|PVYPUI/k});oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ilVL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitG`%9Hbf+mbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkhA1!i]&wnxDJaC6[l#}`s!*Y]w_?&D|4Y5I@ys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L