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' %.*)! 2  2,2 1-84CAG,?&.(" #3"# "  0*)(4      ,,5EA./I+.7j\C@5. %   >L4F>:A!   $$9:@PCI34,4  !+WQJ:HA%  <QWe[]^[>:    52=B681 01XB_[QM36 !B451$  $N_Z\P?8    !()( )/./9%=3@QJJXJOibcRgn^mita[XgR]aLR\VMYOaSAJJ:0(, 1$      ((IMZXN8: %*8NCT/*(    /,1EIBD *#    "C;GGeUkU0%  " 1%<$LL*''5&A(*)4!   &%          # &#  !)+            "      [     .&          X   S !"#$%&'()*+,-.2345678 !"#$%&'()*+,-.2345678(,,,III^^^{{{&&&999DDDXXXaaavvv      ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|{zxQQQIIIfff~~~"""++*333;;;CCCKKKSSSZZZbaahhhooouuu||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 726e11c2521247e6bb92f01211cb7f2aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_17-11_20013130Seq1014410144_Lehman_K1362_trnhjmadera96-Well%K1362_H_11_Epidendrum_polyanthum_trnhKB_3730_POP7_BDTv3.mobSeqPop7z{,<M_u*Qz-[+h X*X`2Z#n-sB~xz#$uR-vbRC6,##,5>FLOPPONNMLHB7* 3Mi%LqU&uL&8eTQ > G OY1 #4 i $. @s]H3~yx|-Lq=x&]8GH<& }Gg yhVD3$ EwCf|w\6pE $"9 (5KTTNJJLPQPMIFEFHJKMOPONJD>70,(&$"                                      6O`ks}o_N=+|qf\SKD?:62/,)&#  !"""!   "$&(***))('&%%%&'())))))+,,,+++-.012246:?CFFECA@?@AAA@?>=<;962.*&$$%'(('$! !!!!""#%(,048;<==?CHLKB2!>y. :/j *=GMSYaiosvz7xa>wy8;z0{{&i|u h$xQMfNVlC( tGi1vQ. (Ge_98Zy%Ov' '  2  @ 98 2R|yrdN3.Y;foF }4hN<0(#  !!   !)069:71*"                                 (3981&   !%*/4:?FMU]fnwug[QIC<4,#    !""#""""#$%&&%%%$$$$%%%%%%%%%%%%$$$$$$#"!      !!!!!!!  "#"$Bf&GlC1Qa^J&t,V*>Tl$A]vz_A"qbZZ`kyvdRC95:FZra=}tlgegn|&T~X,sC'jh^j2,Hbt~qbSE90)# !"########!                                                       !!"#$$%%&(*+-./01346789;=?BDGJMQV[`ejnsz %0=HRY^aejpvz}}{z{}  -:GRY[YTLC90' -AXonAuG{wuuvvvuutsssuwy|~~|{zyz|}~~~~~>d@_s}|rbM9*$-I~I 0 b 8 n H $ Y WQ8-),4@O_n| &-11/*V%iTB4*"!&+18@ITbqmO09bh=}|>m~dL0 i4 ~}|}wnga[UNHB=840,)&$" !#$#"!       #(.37;@EKRY_ejnrvz}~zuoib[TMF?92,&          "#$%&(*+,--.////.,+**)(&$!             3A ںA(,Al~A$iKB.bcpKB_3730_POP7_BDTv3.mobtermAAGGTGCTGCGTGAAGGACACCCAGGAGTCTGCATATAAGACCATTCTATTCGTTTAAATATGACTTTAAGACATTTTAATGAAAAAGAGAGGTATTTCTAGTTTTTTCTTATTCGACTCAATTAAAAGATATATTGTTTTTTATATTAAATATTAGCCATTAACAACAGGAGCTTCAACAGCTGCTAAATCTAGCGGGAAATAATGAGCATTACGTTCATGCATAACCAAACCAGGGGGGGGGGGGGGGGGGGGTTGTTTTTTTACCTAGAAAATTCTATTACGTGTAATCCAAATGTTCTATGTAAATAGATGCTTCTCTACCTAGTGCAATCTACATGAAAAATATAAGCGGGACGTACCGTCATCACAATAGAATTTATTTTCAAGGTGCTGCGTGAAGGACACCCAGGAGTCTGCATATAAGACCATTCTATTCGTTTAAATATGACTTTAAGACATTTTAATGAAAAAGAGAGGTATTTCTAGTTTTTTCTTATTCGACTCAATTAAAAGATATATTGTTTTTTATATTAAATATTAGCCATTAACAACAGGAGCTTCAACAGCTGCTAAATCTAGCGGGAAATAATGAGCATTACGTTCATGCATAACCAAACCAGGGGGGGGGGGGGGGGGGGGTTGTTTTTTTACCTAGAAAATTCTATTACGTGTAATCCAAATGTTCTATGTAAATAGATGCTTCTCTACCTAGTGCAATCTACATGAAAAATATAAGCGGGACGTACCGTCATCACAATAGAATTTATTTTC38Qah{)3;JQYalu#@LVu%8Ddp}!.CYdq} !.9GS]jw(3BQZfr| #/;GP]is)2?KVbny  $ 2 = J W b n y  , 9 E R _ j u    ( 3 A J T _ j z  + 7 B M Y d p {  $ / 8 F S ` k z ,9DR\fs,:HUajw"/8ES^lx %0>DS^lz '2BSYer38Qah{)3;JQYalu#@LVu%8Ddp}!.CYdq} !.9GS]jw(3BQZfr| #/;GP]is)2?KVbny  $ 2 = J W b n y  , 9 E R _ j u    ( 3 A J T _ j z  + 7 B M Y d p {  $ / 8 F S ` k z ,9DR\fs,:HUajw"/8ES^lx %0>DS^lz '2BSYer           3)1$3)&"       "&"#     "+    " +"&"&--"22    &-     """""++   "                         !                 3)1$3)&"       "&"#     "+    " +"&"&--"22    &-     """""++   "                         !      !&\ ; &ME(2 [XT029UdIG*%* )M/FD4,aEU+LU5@\E1_@VG_ ]3'ERD@&2P656;"5&QIQ>?H `dIZa^+@RN -B? /c;.54"MJHQD Z*K2P6KB_ N V: ZR[O^WdBC[/DE! 4QdFE C:1]`c@ HI6?C]H/E5RO $/)45Ta'.JFF2FF;1]&SH>3`)Va&J2:^c!;\VVFH2E` DT#E,aS' K9\TaSEBE-$Y2\6N22E;8CE203&J\ZGD2V2?3]CQEW:$!#A2L$*K&-L= 2(@D+4EMLT6=''"2] ?@x74% DM|403<yOwoU YP)j T qRoXtl  K@+w E 8S2z? c1NAG|c#$g^+'mv a<%%J?TtXu70t  FAq4Us ?%E x\?@lr+{+G 4f+IH} ]UQ[Yf,t"(o{IMAi;Ow$4RF zX q/h @`dC !z`"zbmHJ @@&`W';vBJ)DU@$P1DiAw`- 7Iq%<P!b=]ZSgx10W *,+ A  1 / #9  gS,1pc Tp&ms*7N3p2Jm[1SWa7/e/p '} p}`j'iaA%(tpk HsV<D".Tfl| ,^Z&u}@dt 1~rbt9 ;NK8&Wjfgk`pWxV@8,h,lh+1g +1Dohm &f[Nr 1N\}W3Lh[S(0ETl 8q?k. =(N Q T wm~?OZX ,rUnRp'$I}XnD&CRW+|$}{2{ldMh f:y3`O -e9+Q7Mvb6Yb&LQ`OyN{rdaj}&+n7<-!,(a<UvhWEZqs0jzFy ~Od)~fWt.4_m}Y_d\j(gQ;T5NZz^RK]!$U<>7h|O r&.=.!7 .YMh lGGAAGCGCCGCGTGGAAGACTTACAATACAGTACATACCAGGATAAAATGCTCGAATGGATGACTCGATCATACCCACCCCTTGGGGAGAGCCCCCCGAAGAAAAAGAACAGAGTCCACGCCCAGGGGAGCTCCGCCAGGGGGCATAGGGCAGAGGAGGTAGGTATCTGATTTTAGGCCGGTACAGACTTGGATCACCCTTCATTGTCAAGAAGACAGTTGTGTAGTGAGGTAAGCTTCATAATTCACAACCTTCGGTCCCGGAGTTTACAGGTCACACCAAGTAAAAGGATAGCGCCGCAACCTGGTGGTAGATGGAAATAGGACGTGAACTAGCCTCGTGCTTTAGGGCAGAAACAAATAATGAGGATTGGATAGTAAGCAGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 18:42:49 -04:00AEPt3AEPt3APFN JAPXV2APrN APrV1APrX}}ARTNASPFASPtOASPtOB1PtNB1PtNBCTSABufT"DCTIDݛCTNMݡCTOwݹCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DBDATA"DDATA"DDATA 'O.SDATA 'O.5DATA 'O.DATA 'O.DCHTDSamDySN åDye#DyeNðDyeNõDyeNúDyeNÿDyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT FEVNTREVNTeFWO_GATCGTyp!!ÄHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~?LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS#OfSc4.P1AM2dP1RL/^P1WD5jP2AM;vP2BA>|P2RL8pPBAS$PBAS#FPCON-PCON,XPDMF3PDMF#*PLOC)RPLOC&LPSZE`PTYPPXLBRGNmݓRMXV1RMdN }RMdV1RMdXՆRPrNaRPrV1RUND RUND RUND RUND RUNT  $RUNT )RUNT "RUNT ()Rate xRunN""qS/N%# SCAN3SMED SMLtSMPL&& SPACApbSPAC##SPACApbSVER4.0SVER A}SVER,,Satd4^Scal@Scan3TUBEH11Tmpr<UserphARphCH#AphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN JAPXV2APrN APrV1APrX}}ARTNASPFASPtOASPtOB1PtNB1PtNBCTSABufT"DCTIDݛCTNMݡCTOwݹCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DBDATA"DDATA"DDATA 'O.SDATA 'O.5DATA 'O.DATA 'O.DCHTDSamDySN åDye#DyeNðDyeNõDyeNúDyeNÿDyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT FEVNTREVNTeFTabOFVocOFWO_GATCFeat..OGTyp!!ÄHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~?LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS#OfSc4.P1AM2dP1RL/^P1WD5jP2AM;vP2BA>|P2RL8pPBAS$PBAS#FPCON-PCON,XPDMF3PDMF#*PLOC)RPLOC&LPSZE`PTYPPXLBRGNmݓRMXV1RMdN }RMdV1RMdXՆRPrNaRPrV1RUND RUND RUND RUND RUNT  $RUNT )RUNT "RUNT ()Rate xRunN""qS/N%# SCAN3SMED SMLtSMPL&& SPACApbSPAC##SPACApbSVER4.0SVER A}SVER,,Satd4^Scal@Scan3TUBEH11Tmpr<UserphARphCH#AphDYbigphQLcphTRphTR-Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&rYA z3]qgi8$mzARW/z|s:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]44+.bi|!dk}/ .NPaz6IB]+H4A|?{XU/7/~.|f&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pMCWk>6GStN44 Jf1Q;gVp[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQno X:QJf9/qwAxew>6?|$lQ%:XGTtrWV*AEPt3AEPt3APFN JAPXV2APrN APrV1APrX}}ARTNASPFASPtOASPtOAUDT_B1PtNB1PtNBCTSABufT"DCTIDݛCTNMݡCTOwݹCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DBDATA"DDATA"DDATA 'O.SDATA 'O.5DATA 'O.DATA 'O.DCHTDSamDySN åDye#DyeNðDyeNõDyeNúDyeNÿDyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT FEVNTREVNTeFTabOFVocOFWO_GATCFeat..OGTyp!!ÄHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~?LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS#OfSc4.P1AM2dP1RL/^P1WD5jP2AM;vP2BA>|P2RL8pPBAS$PBAS#FPCON-PCON,XPDMF3PDMF#*PLOC)RPLOC&LPSZE`PTYPPXLBRGNmݓRMXV1RMdN }RMdV1RMdXՆRPrNaRPrV1RUND RUND RUND RUND RUNT  $RUNT )RUNT "RUNT ()Rate xRunN""qS/N%# SCAN3SMED SMLtSMPL&& SPACApbSPAC##SPACApbSVER4.0SVER A}SVER,,Satd4^Scal@Scan3TUBEH11Tmpr<UserphARphCH#AphDYbigphQLcphTRphTR