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(/  c\fM@UO9& Pwa)+ <#*(+..+L&  U@,%Gx 2B"8%('+- ../U/}2:49 ?IU6agkmpsPuvIww#vut:rwomKlkiigf_dc}`\|XUMC8/j'#R&6eMMpO+ Fp^Z!Wt  A  h v e  W 8 & 0 U  &JQ5 : 7 A -t9RSDrfu s7y: G , Jcd0] c d o #*v? t`h&  !y*:Z|g_o^`ecX{CkD7XF:>+&<.0!# hhrxsyiTxlsZa^WdbaJVe__M__jOz9MKj[SR5S>[D6?D<ATHAD3.+51A-0&0,0/"# !&)&'&'!"&%)*&,  &    ) #' ),  !    1(!#  -47=4A7D8-*%        ) +,8        (+#+$16C=EDDD2:+,-% % $(=2@0D^[BKbpTWt  u i 8 5 RV&ANAT!H K ; ,'M-a/N@ nwfdSah|i~cr|wlnUn_[Wh_jXS[RWU9(JnMuwlPzt`yav kgn]jqjX`UUHHN\A]@BAE*4GB9GYJbcMnhMBRD::IW]c`ma^D.B83B-+9;ES@AD3&# %;)NKC3       !5(#      +=;FG3n}u QDoV}I7h K d   =v<af^UpQ"mGH=J$*+ '*/=7N9;MWL#*./.E@>M9>%? ) "1 +,E9;57E@+FYhVplp^iW\IN@_`5B?zcPCGdQRcqRc[hicK>OEoVc}mX]P^N[I?P6VD2TG/DWN*N6!0-07'GD3VI?XMdeI51+4+ *#!2$*,    *   @11-ZIWptm5#GCzku !{x`NB/q``omJK<#.     !&'38DK!   j# !"#$%&'()*+,-./0123456789:;<=>?@ !"#$%&'()*+,-./0123456789:;<=>?@(,,,III^^^{{{&&&999DDDXXXaaavvv      ***3>>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR|{zxQQQIIIfff~~~"""++*333;;;CCCKKKSSSZZZbaahhhooouuu||<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1b9d6e30821b40f49bbf66a1f1e166caSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_17-11_20013130Seq1014410144_Lehman_K1362_trnhjmadera96-Well'K1362_E_12_Gymnosiphon_divaricatus_trnhKB_3730_POP7_BDTv3.mobSeqPop7z,s_8C I @ nVm2!$R&)q,$.158z<?C.FJM}PTkW[]`(bcldte)eeeMdcza_]ZWTQMIFQB?;8652 /X,*(&%$$J$X$%p&7''()l*Z+X,V-=-......Y-,~*)-'.%*#E! 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"!     #$!'B^u{jU@- %+02211124568;?CEECA>==??>=:989;=>>=;9520/000/,(#                                                                                  A 7LAWϫAq_A+KB.bcpKB_3730_POP7_BDTv3.mobtermAAAGTATACGTTACATTGGTACGTCGCATTCTCTGTCGTTAATAGTTAGATAATAAAAATTCATAGAGAAGGGAGGAATAATAAAAGGGGAATTTTTTATCTCTAAGATTAAGAATCGAATATCTATAAATTTTTTTTTGTATGAGTACAATGTTACTAAAAACTAAAAAATACTAATAATTGAAAATAAAATTAATCACCAAATAGAAAAATTCTTGTTGTATGAAGAGAATTTATTGTTGGGGGGGGGGGGGGGGGGAAAGTATACGTTACATTGGTACGTCGCATTCTCTGTCGTTAATAGTTAGATAATAAAAATTCATAGAGAAGGGAGGAATAATAAAAGGGGAATTTTTTATCTCTAAGATTAAGAATCGAATATCTATAAATTTTTTTTTGTATGAGTACAATGTTACTAAAAACTAAAAAATACTAATAATTGAAAATAAAATTAATCACCAAATAGAAAAATTCTTGTTGTATGAAGAGAATTTATTGTTGGGGGGGGGGGGGGGGGG 9Sd#0HYjs #-=KTis  4=OZfrz'1<GP]fpv%0=LU`lx'8@KYfo|&19GQ\hs~'1>HS^gv   " / 8 A M X e t     ( 3 < G T ` j v    & 1 ? G R ^ i s }   9Sd#0HYjs #-=KTis  4=OZfrz'1<GP]fpv%0=LU`lx'8@KYfo|&19GQ\hs~'1>HS^gv   " / 8 A M X e t     ( 3 < G T ` j v    & 1 ? G R ^ i s }                 ,>,           "  $      '  "#      ""               ,>,           "  $      '  "#      ""-2GWTY+^ #Y`T][&H+^G:<MY>F ;]? AbG-DU9c: /Ccb% 7(YE>`4?`RJHaP:^M@TU"P"Fd ]]RLIbD!,W:KX: aR7`6\DIZ;b =1 /2J+:D>Hc NF"RE'5WC `aX2 c2N(4$@])$@]Hb_2N (@'&NA 3B`@9)+$N4M3K1Jb0Ha91<,v!I D (Il &+*_6ix.x&86  f[ z \SntAa$xO (r3 {Q1 a&.dI(9t<NXeev`9}A f  A}qp!>8[=TbX2%7*W!^[s jn"@-BYac d$ H^kcgn_maqWC={x'c, {k9x2h H H H  ,&@]r8_f5 |}k K{ ZucpToIg3gND3\;"$#N<(PG  1?>Z,94,"Kg wt0R-prplC9e`JKi~W3pdC|Tl2`j/NFJLD5hCNLjd"d}zsVeWOt|,tt|xuSV 7Q&s*W6.06 5(Td{{/9|DmV Wy>_:{'w|Y:*>;Uux4 ?6Vn~\$"5 53U3U*&Rn j[bpR=y:Xq[R U >' !Z"}UE9y0~|HjD979PQ3\!Fp$E (RG~I"E8K*<qO"pJRUy%;1'&% CGGAGGCGGCCGGAGGGAAAGATCTTATAAAAAAAGGANATTATTAATATAGTAGTTTTAATTCTTTTTGCCAGCCCCCCCCCCTTAAAAGCCCCCCCCCTCTACTAGACCCATTATTTTCCCTCTAGGGAACCCCCCCTGGGAGAATTCTAAAAGTATTTTTAGTTCCTTACTGCCCTTAACGGTTATTTCAATTCGCTACCCAGAGTTTTACAGCAGATGGGCGGAGAGTGAATAATCGCAATAATAAAATTATAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 18:42:54 -04:00AEPt3AEPt3APFN APXV2APrN gAPrV1APrX}}pARTNASPFASPtSASPtSB1PtRB1PtRBCTSBufT"D9CTID CTNMCTOw)CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DnDATA"DDATA"DDATA"D:DATA #GLDATA #GL.DATA #GLu]DATA #GLDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT êEVNT öEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEXLAST~~iLIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISOfScBfP1AMNP1RL HP1WDTP2AM`P2BAfP2RLZPBAS3PBAS0PCON EPCON BPDMFPDMFPLOC<PLOC6PSZE`PTYP1PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  $RUNT )RUNT "RUNT ()Rate RunN""S/N%SCAN3SMED SMLtSMPL((}SPACMSPAC SPACMSVER4.0SVER SVER,,~SatdB^Scal@Scan3TUBEE12Tmpr<UserphARphCH+phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN gAPrV1APrX}}pARTNASPFASPtSASPtSB1PtRB1PtRBCTSBufT"D9CTID CTNMCTOw)CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DnDATA"DDATA"DDATA"D:DATA #GLDATA #GL.DATA #GLu]DATA #GLDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT êEVNT öEVNTEVNTFTab'LFVoc';FWO_GATCFeat..' GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEXLAST~~iLIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISOfScBfP1AMNP1RL HP1WDTP2AM`P2BAfP2RLZPBAS3PBAS0PCON EPCON BPDMFPDMFPLOC<PLOC6PSZE`PTYP1PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  $RUNT )RUNT "RUNT ()Rate RunN""S/N%SCAN3SMED SMLtSMPL((}SPACMSPAC SPACMSVER4.0SVER SVER,,~SatdB^Scal@Scan3TUBEE12Tmpr<UserphARphCH+phDYbigphQLcphTRphTRV"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W;!'8Agzu]^H8L$7$% WM\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUVd'k)bebyI)YGrj\,>JB0#Fm4f)yAIpBd $D&,6ZMR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4ZP}Gs,"T'oJ-omMOx+6T,IL|2D5^.4*WAH>W;V>UfuJB,jHbinOY5_}4]'>'s1HTf{fzja:3AG}X`=/r.ZNum#0kc "7:I12gbXW,62Q$OEHbX\"B[M@{`29w/>r,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*