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(  )' )!(!" ! $*$%      d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2c89c4c3b2da4371a0bef06faae99957Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-Well!K1361_H_9_Moussonia_deppeana_trnhKB_3730_POP7_BDTv3.mobSeqPop7z../147:<=>?ADHKNQTWY\_behlpty)3>ITan|!(0:DNUZZWRLHC?91& whXI9* "#$#"  zslfb^\ZXUSQPOOPRUWZ\^_`abbccbb`_]\ZYXVTQMIEB?<:9641.,*('&%$$##""!  !""#$&'*,05:?FNWbn{.EZnt[B*xrmjiilpv} /Z2rYZ Nr_,TU^jA|wspmkhd`]YVSRQPPQQPPPQRSVY[^`acdfhknquy #?_9]mEY-}wspnlifb^ZWTRPNLJIHGECA><:8631.,++,.048>DLT^gpxzupljjknprttuttsqpmjfc_\YXWXY[[[YVSPNLIFB>951/..-,*)('(()*++,./01124579;<<<<;;:986531/.,,+,,------,,++**)((&%$#""#####"!      (@UaaUB+   "#%'+/2469<@EILORUX[^adgkosx{~}ytojfa\VQLHEA>;72.*'$"     "&),.147:=?ABBCCDEFGHHHHIJMQW^elsz,>MYbinrttpgXD,vnkmv1b99q{pq|,Txq=l1sdXOGA;60*" ziVC/yqjd_ZWTQNKHECAABCEGJMQV]dkrvxxvspnljgd`\XTRPPONLJHGFEEEEEEFHKMQUY\`bdffffedca```adgkou{%HpB^q{|saH*raUNLOU^hr|~p`M:( ~l\MA6-&              "%"  !!!""##$$$#""!      )7GXhw  1>JU`jsvsiXC.    uZG=<BKV_flry *7BLT[bhmrvxyxvspmjgd`\WQJC;2)~|xuqmid`\YWWX[_ciov}|ung`YTNJGDCBBCEFHJKKKJIHGEDCCCCCCCBBA@>=:851.+'$                          !#$%&&%$"      !#$%&')+.13679;=?CGJNQTWZ^chmqux{~~zwspooqtx|ymbXPG@94.*'$"! !$'+.147:>CGKORUX[]_abbbaa`_^][YWUSRQQPONMLKKLMNPQRTUWZ]aekrz%;M]jt}4q jL><bw|vm_N:" zU+uIg#tK*zocWI;- ~xtrqpqqqpppppppomkigfgilry~zwutsstttssssssrpnkgb^XSNIEB@???><:852.,)&$#!  !"#$%&'((())))('&%$#$$%%%%$$##$$%&&%$"!           "&)-15:@EKPTY]aeilpsuxz{}}|{wsokgc_[UPJD>940+&!   !#&(,/258;>@ACDDDEEEEFEEDB?<962/,)%"       )XA^AW?AUjAKB.bcpKB_3730_POP7_BDTv3.mobtermTTAGTCCTCTTCGACGAACACTAGCATGCCCATCAGTACACAAACCCCTCTCTTTGATTACATTACTTTTTTTTTACTTTATTTTATTTTTATTTAAAAAATTTGATTTAGTTTATTACTTTCTGTAATGGTTAAGTAAGAAAGTCTAGGACTAGGTTAAGTACAATACTAGTAAAAAAATACTAGTAATTTGAAGGAAGTAAGGGCCTCAAACCCCCCTTGATAGAACTGGAGAGAGTTTATTGCTGCGGGGGGGGGGTTTATTCATATTCTATACTGACACTAACACCAGTCTTATCCATTTGTAGGATTAAAAAGGAGGGAATCGAAAGGAGGATGATGATCTCCCAACAGGTACCAAGCAAGAGATTTAATAATTTTTTTTAGTCCTCTTCGACGAACACTAGCATGCCCATCAGTACACAAACCCCTCTCTTTGATTACATTACTTTTTTTTTACTTTATTTTATTTTTATTTAAAAAATTTGATTTAGTTTATTACTTTCTGTAATGGTTAAGTAAGAAAGTCTAGGACTAGGTTAAGTACAATACTAGTAAAAAAATACTAGTAATTTGAAGGAAGTAAGGGCCTCAAACCCCCCTTGATAGAACTGGAGAGAGTTTATTGCTGCGGGGGGGGGGTTTATTCATATTCTATACTGACACTAACACCAGTCTTATCCATTTGTAGGATTAAAAAGGAGGGAATCGAAAGGAGGATGATGATCTCCCAACAGGTACCAAGCAAGAGATTTAATAATTTTTT(.O  @GZ^o"2;EKScmz*>MZ`v$3AO]kr}!3@Uer,9DRbp~ #0<HW_ip~*6DO]gs    ) 4 A O Z e p |    ( 4 @ K W e u   % 2 = H R ^ j u    ! + 7 ? 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I T ] i s    ' 1 : D N [ n "-6ALS_iv~ *3=GS]jy!/<HRZen~ !,7HSbmv #0=HRbkz      #         .   %%         '  "  ""     ""8+#"         !                                  #         .   %%         '  "  ""     ""8+#"         !                            c +-b ;cN@EBL3_ \ ; \G[ +#R 'E=VdOXU.? acS-+`F'D<\ C; F V3$Z]]]3+8 15'I WZ^D^ =8@H]S7L3IGL*_bV 3:@cT;T ]c*P`K0J.6F WF>2(3AB+ VZ!2 ]RGcWV:%DRcLAc]1MRB[A1Jd^K:*Nc.Z97YbEXW$QF6S[ZEIOEB@><97530Ya8,(Z2YYX/_I,G2S 8,#)&"Q8[!T.P Z8&>M](#G /G&2>2'2L&>&>6&>> 22>c>>&G>+-2X'K W>21,28049&>>-Q>G7"J G0~ d@#Pt)$s = 6,8 b0q\@gaK9l< $/nV29 7 q  94w(r([ga$n)!8D k ,Z:*c#%3]`@}r_N~`-gBtxlakCT_w #` )r F _ J asB0IQ&Xd} p_9mZ[UQHjp=s\u"ZXs^/#T_0]J'/7!Z5|NWo`T_r/%lZ=U\=$y x2+OncMvLqY -$  O@ P#9hN5 HJ& }nebT`6| OBTG B`bp]APX```RJ6)+=(SviO \v@A6 oNcO4NB_F/AnlBK  pppp},`>ry#0p9:*o* db>>lO%JAm>;;"ipdS.s p&e ]8/m:&.,#+j}ppY22 A%>a#q y[dW@Tp<nk7F111QwjK yB&p/)^]9;8;>W[d4`i #/'IX#m ybPW  cK"|597_8w8fwbKyy-I=N(7YxSR0<Bo)e8Up5 0!*+W'12/cW}u,H\3^ CaEC;(0n;+S5GQGk:e'Q5pQG:($9)#GX8Z 6z|CDy~"uN J-)Ru'xT JE U3@~xt/%9rZ6C[/5GqM  stgj_0JIH0Q@AGGAGGGCGCGGTGGAGGACTCCAATAATTTTGTTTATACACCTAAAACTAAAAGTTAACACCACAAAGACCACCTTCCGTAAGCCACCCCCCAATTGGGGCCGCCCGATACAAGGAGAGGCGATCTCCCCCCCCTAGGAGGCCCGCGTAGGAGCTACCTTCCTCCCTGATTCTTCCGTTCCAACCGCTACAAGTATTGAGTTTTCATCACTGAAGATTCGTTATTTTAAATGAGCCCAAACCGAAGCGTTTTTTTTATGAGTCAAGGTCCACCGTTGTCGCACCTGTTATAGTAACAAATAAGTATTAGAACTGTGAAGTATGGAGAGCCTTGTATACGGATAAGTTTTTAGCTGGGAGGCTTGCTATAGACCACTACACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:10:14 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTSBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA =DATA =#DATA =aDATA =DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT šEVNT ¦EVNT²EVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~GLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISݧOfSc P1AMP1RLP1WDP2AMP2BAP2RLPBASYPBASPCONSPCONPDMFPDMFݾPLOCPLOCPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%ݟSCAN3SMED SMLtSMPL""mSPACMSPACݷSPACMSVER4.0SVER SVER,,nSatd ^Scal@Scan3TUBEH9Tmpr<UserphARphCHphDYbigphQLcphTRphTRꁅThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt"ASPt"B1Pt!B1Pt!BCTSBufT"D)CTIDCTNMCTOwCTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA =DATA =#DATA =aDATA =DCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT šEVNT ¦EVNT²EVNTFTab *FVoc FWO_GATCFeat.. GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~GLIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISݧOfSc P1AMP1RLP1WDP2AMP2BAP2RLPBASYPBASPCONSPCONPDMFPDMFݾPLOCPLOCPSZE`PTYP!PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%ݟSCAN3SMED SMLtSMPL""mSPACMSPACݷSPACMSVER4.0SVER SVER,,nSatd ^Scal@Scan3TUBEH9Tmpr<UserphARphCHphDYbigphQLcphTRphTRAvotqUa]KOfx&i1PBEe>2}?6i<#~p$F0Ns>yEOikaw>6?|$lQ%:XGT8P/kdSPcgoC#Q9p_]c3-doFOACEDFi34TKs;6R&w^LgdOZR<0+M??^V$Y#L,jpJ6~=D:WqPKw0j9eKobs>6IxO\JpSbwEiv58u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN+b)c7W`XD8NT !T;b^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sb: h4qKl}T2#:Da^?vftu3ky+MGPpF~qoMY'H\W+|v^.PZPBE YXp!R*lVinJilSZrB]'G,lwdRadJsV&=o?eU&GUkJZm{n(c%H?b+]d:?hZ2h$qiE>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E~$3.-Zl7'p=R .*}Lq{F}u^zq+|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[gLYCBF#TeA:AkR:6i`vqFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdA1*+=<)s tnjJm}e7#LhN@[*K+O:<>.yc7qU+NU[xinJilZbCd]'p8Npdpu#Wc4hAC?-4$&FkNp&iJ-omMOxjYa/k#Pb~;H26t{_"#kI>b0FcS==6X12Dg8YauBQBhwD~EG7&o.janJiRzq`6\qwHN02T(vW3TA=]Ik'2g|ea}wKShKn6g;X6YM%gcmeY_@h.<$.^ka@GpSC>lVm? ##k0$y!TW3nG6`VX+*flVOY(xs/b@j/.cgoC#Q9UP_`G\U5ZfoQe=N^tpu&ofW)NKo-j2Z.bB E:Yh^n@EK5llme