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"    !  " $  #  $ !!   !    d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8fe9136133cf455bb177335bb1b40108Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_G_5_Oxalis_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7z  .Ir;c\Ufc 0 ^ .]:a YIi4<- & Q {^@8Ir= <   [ X ~  \(>I|os .Hczu^E.zKv[B, #/>PkV !Th$ ( 1 < # $ G"u wF% 6m.sVt 7+"',04768O9/999B8b64%0,-'J"KCo x 6aFvB{j^YWXXXZavB8>|@VO*$X(y.!P(m{TRu5ee-sG#{{,p<EAlr?%"0EXaX< -2lWJC??@BEIOU\dkquwvusrqppomje^WOG?71,(%#"!"#%(+-../28BMW_ccbcgrb zOqH- '/7=@A?;72-)$ "%())&"        +8BHIE>5+"!)29==:4-&     &RFIX0 #&(*+,-./00/-*&!  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;P:.LI;/F*Q1@#M4_4+,?;%b:D8!b)JYO9(SWQR-Q [SAAc7B+@5,&#*E&5>KT5, 6_@1a#5L3VL U" -AQ5+GARN6/ =BVJE&R0E6 dr& {.r -_Xqdipy|B}@@T-mp'{.O0^r([]Dk(7QAeq2VhB? cQ>,<4Ce7!)n^}p^>x~*I?;&F eDrf)\uo5 Lu%5:n.>]Lfr`k\#+GEqU<rdz;uROq.AAC\yL?^We H.s++h6UBtkE4YHTgS>>T;<2<%"@ 4YF%!'-  x"]!/#  C (     '/ )ppJ 5xp<R"c 8$<6_I158pUNPE8wYR]?O9bW9L*)dyPKRBFd6.R@|1ypdSp{O\Q/E8Z^kHD8d}p*q8, I(w8ojR \WB ?% ..- 56W [4X.C e#=<YBxG (8Pk(8TvI:1e3 59!(4#&GN'.4(w+~QiKK8(EK!70 ' !+$""6?'cYv9-(5:(((  G  '$6# ! #/ 3 -f\Pi^>  &2#*).%#K&2$ !I,3('*?39;RSfX}Zo]|K@ C0'%t+<!Z#=YU@QE6Cd$75><&4E 96D,?*)#[JJIUL7WS7GS0AGGAGGCGCGCGAGGGACCCAAATCCGGTCGAACCTGCGGAAATTCAACAAATATTTGATATACAACCGGGAGAGCTCCAGGGCCGGCCCAAAAAGGGCGCTCTACTCTCCAGGCCCTTCCGGAAGCCACCCTAAAAGGCCCCCCATTTCCCGGGCCCTGGCTCCTGTGTTTCCCGGTGACCGGTCCCGAGCTCCTTCGAGACTCTTCCCCGGGGGTTTGTATTCCGCTCTACCACCGCACCAGTCAGGGGGGGGGAGGACACCCCATAATCACTGTGGTCGAGGCTGGGATTGAGCCATCCCTCGATGTTGCTTGAGGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:09:45 -04:00AEPt3AEPt3APFN APXV2APrN oAPrV1APrX}}xARTNASPFASPt ASPt B1PtB1PtBCTSGBufT"DACTIDCTNMCTOw1CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA ) RDATA ) R7DATA ) RDATA ) RDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ²EVNT ¾EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~FLIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOIS.&OfSc#P1AM@:P1RL@8YP1WD@=YP2AM@BYP2BA@@DP2RL@?PBAS@@/PBAS@@.YPCON@@7PCON@@5PDMFPDMF.=PLOC@3YPLOC@0PSZE`PTYP9PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%.SCAN3SMED SMLtSMPLSPACMSPAC.6SPACMSVER4.0SVER GSVER,,†Satd#^Scal@Scan3TUBEG5Tmpr<UserphARphCH.TphDYbigphQLcphTRphTR+@This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN oAPrV1APrX}}xARTNASPFASPt ASPt B1PtB1PtBCTSGBufT"DACTIDCTNMCTOw1CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA ) RDATA ) R7DATA ) RDATA ) RDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ²EVNT ¾EVNTEVNTFTabUFVocUFWO_GATCFeat//UGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~FLIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOIS.&OfSc#P1AM@:P1RL@8YP1WD@=YP2AM@BYP2BA@@DP2RL@?PBAS@@/PBAS@@.YPCON@@7PCON@@5PDMFPDMF.=PLOC@3YPLOC@0PSZE`PTYP9PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%.SCAN3SMED SMLtSMPLSPACMSPAC.6SPACMSVER4.0SVER GSVER,,†Satd#^Scal@Scan3TUBEG5Tmpr<UserphARphCH.TphDYbigphQLcphTRphTReZ>\&bD=8d)sGi=&MQJ1rpJ=&F)9OHUkg;V<@Qn&;|>S8pu_Zj&[[Rq&!q)~'pEA({]/AT^ogqlp,D~lk;)+S8UXabCjC:AVVu>zKVJeoiQ]yw/b@j/.$I _|!{l29O[m[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{ce+bS_?Z d~)*$Fs{`jC52#GN3KHU;HBIc+75i@TH/JBcwZOHte?tH"D3|>oW~!.eHB,}sv&'Yj}4]'xP!t p7swnqR#$|Nq_{U3UbKSZDJUzp_iSiVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_ K*Rh ~y=NAm+iw<+)YoTGYuB^9~Ky\rm#ulc*{d&]?#(eaK|`P@R(RlSR5y=Y`gXBY'^n7a;A{%i7#W yZE!e~8WYH|O[nYL.wJiBwH~?p MJM*{B]9[ikI0o6Gzv&1`Zlc*+?_u';fQGKerctp(kA;M4[!f<,hm<7eaAvG9 HtWo0g.W3DK]T5^.?s|"Ff61}XTu.ycI0 r#U43*nv&pf~{&qDFin5&5OyX0YbzE%L(!f|bEdGKI'_26.?bFW_E7bp~4o-D7I 9_[*?bCDkrD5pY^d,/IQ}{sGitGKXW .CTNCwD(ftyYZFUI8-W=;OF>BrRL8~f)NKo-bEd9gP`gvG9 b?AvQtb'J!>yH~~!|s&4)D@xG57'9\47.<<_r[)PCy5"igxd}s9?Od+k'"5-z]G3o6Rl6%4y^.EAx8aPI^QJH){!9Y2]2f1hu?K2FtSEH$*YYo%@rs{6`VsIeJM*{B]9[ikI0o6Gzv&1`Z=kY+-^tG|JWoa$Fl@i2<`2fvn[?a9TghW~^N@.]neZ_kjh'SQAEPt3AEPt3APFN APXV2APrN oAPrV1APrX}}xARTNASPFASPt ASPt AUDTeB1PtB1PtBCTSGBufT"DACTIDCTNMCTOw1CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA ) RDATA ) R7DATA ) RDATA ) RDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ²EVNT ¾EVNTEVNTFTabUFVocUFWO_GATCFeat//UGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE#LAST~~FLIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOIS.&OfSc#P1AM@:P1RL@8YP1WD@=YP2AM@BYP2BA@@DP2RL@?PBAS@@/PBAS@@.YPCON@@7PCON@@5PDMFPDMF.=PLOC@3YPLOC@0PSZE`PTYP9PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%.SCAN3SMED SMLtSMPLSPACMSPAC.6SPACMSVER4.0SVER GSVER,,†Satd#^Scal@Scan3TUBEG5Tmpr<UserphARphCH.TphDYbigphQLcphTRphTR