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d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 823396bdea664feaadd353f685df8ae9Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_F_8_Piper_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7z88:<@FKPSW[`flrx~ +<Nat +:GRZaehhfaZOB5&~gQ>+  (.256641,& wohb^ZXVUSSSTUWZ\^_____^]\YVROKHEA=951-*(&$"! !'.7@KXgx "9Nap|zlYC) tdXMD<732359=DLVdv![;JUE5uV* ytnid`]]]^_acejov}?oOR%($u6Tc#  |uojfa\WRMGB>94/+(&$#"""$(/8ETf{{squ~ztpkf`YRKD>9520.-,+*)(''()*,.147:=@BCDDDCB>93,&   (19@DFD@91)      !)/341,$  $*..+&  #2@KPPI=.         '++(!                                 $%$    qrtx~{vqlfaZTNIC>:51,($!     #&()*+++**)((()+-05;BKT]fnw}{{~/BNQL>*Jv 1Ox!HS= u0jL- `C+xka[XWZ^bfhiigfedegjmqv{~yvvx}  %%$   <m mG4L@`{ Z'IhnU9lXG9.$  !)057750+% "(-010-("(3=EJLJD<3)!   &+.00/./26<AEGFD@<8666665420015:?DFFD@;74334542-&   $-6<>>:4-& $*-.+'! )8IYfnqlbSC2$ !.<ISXWPE7( !*1552-%   "(,,)$         $(($"$$      "   !+241*  !"    "!  #),+&  ",4872) #+/.(   (--)! ##   !  #%$    ##                                                                                              !!!       6Rk.86&eOGHPZcjnqtvxyyyyy|4Lcw|kXE1  {ww{{iWE6*"!&,4<ENV]behiihgd`ZRJA91*$!(08AIOSUSOIA7.$   #'-4;BGJJGB;2*"!'/6<ACDB?93,%&-37974.'  (19?A?:2*!  $-5<@@=6.%   &.6;=;6/'! '/6;=:4,#   %,//+$%/8=<7.$)28960&  (3:=<5+  '3<@>7+  +7@B?7+  $$!  %-253-%%.585/$ $+01-%  &0796/%  *265.$ ")--(   '.0.'  $,241*! "/:@@;2'%0:@B>6,! (1784,!  %))$ !(*(!  #)*'! $&$   #(*'!   #%#  %&#  !%$   #%%  !')'!  "%$  "$" #$   &*+("    #$# &+,)"       #$  #$" !"''$$%"  %'% "&&!  )..)  &,.*"     !'($ ""  #  "" #$!  #  !                                             !$'+/38=BFJOSW[^bfimpsuwyz{{zyxwusqomjgda][YYZ[]_bdfiloruxz{{ywsokfa[VPKFC?=:86668<BHPXajs}ugZNB7-$  !#%'(()))('%#   &/:GWj~ +;Nj/?jyui^XXYWM8vZG:0#o5eJ>@MayqYA) ,7@DC<0 ytqpqtw|vdTE;30/16;AGMRVXYYXWWX[`gr~ xne\TMF@<741//26=EMTY[[ZXVTTVXYZYVPG=2'  %/:CLRUUTROMMNPRUWWWUSQONMNNMMLKJJJIIFC=6.% "*27:95.$ ").10,%   ,6>BA<4)     +5<=:3*!'08==90&    .<FKIB7) )5>B@9." !#&*-037<EP]kw~uh[NE>9630-*'$"   #+/0,&  %+.-(  &0561( %)($ %**'   %,021,$  !   #"  ""  "'(% "&%!             4RAAMA]AvKB.bcpKB_3730_POP7_BDTv3.mobtermGAGACGCGCTTGACGACTCCACAGTAGCGTACACATCCGCCCTATACTATCTAAATTACAGAGATTTCCATTTGTATTCTTTCTTCTTTTTTTCATTATTTTGAAGTTTTTACTCGTCTTCTAAGATACAATAGACTAGAATTAAAACATTCATTTCTCATTTTTTTTTTTTTTTAAAAGAAAAAGAAAAATTGCTGGGGAAAACGTATAAAATAAATTATTAAAAAAGAAACAAAAAGTATGAAACTCCCATATTTTTTTCCTATCTTAAAACAAAGGGAAAGAATGTGGAAATAAATACAAAATTGATGTGAGGGACTACTCAAAAGGAAAGGGGCGATATTAACCCTCTTGTGAGAACAAGAAATTTATTATTGCTCCTTTTCGTTTAAAAACTCTTATACATTTAAACCAAAATTTTATCCCTTTTTAGATTTTTTTCTTCATCCCCCCCCAGGGCTAGAGGGAAGCCCCCCGAGACGCGCTTGACGACTCCACAGTAGCGTACACATCCGCCCTATACTATCTAAATTACAGAGATTTCCATTTGTATTCTTTCTTCTTTTTTTCATTATTTTGAAGTTTTTACTCGTCTTCTAAGATACAATAGACTAGAATTAAAACATTCATTTCTCATTTTTTTTTTTTTTTAAAAGAAAAAGAAAAATTGCTGGGGAAAACGTATAAAATAAATTATTAAAAAAGAAACAAAAAGTATGAAACTCCCATATTTTTTTCCTATCTTAAAACAAAGGGAAAGAATGTGGAAATAAATACAAAATTGATGTGAGGGACTACTCAAAAGGAAAGGGGCGATATTAACCCTCTTGTGAGAACAAGAAATTTATTATTGCTCCTTTTCGTTTAAAAACTCTTATACATTTAAACCAAAATTTTATCCCTTTTTAGATTTTTTTCTTCATCCCCCCCCAGGGCTAGAGGGAAGCCCCCC4J]f~,9BNZdjw #0<N_gs|  *6FP`iv .;FS`kx+7DO[ht!+6AP[fp{(4@KWbn|)5BNZfr|  # / ; G T ` k w  " . : E Q ] i u    ' 4 @ K U b l x    + 4 A L X d p }  ! 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3 F=( (  ( %2 1&.2! 1"* $E %),      !E =;#9A05 C/dhXbpp*GvHa-CS"8g;i# TH\;~Gox pRujd)_xpO8w[ *<i89M2t B_>>ks8wDEm|E!mL(W_I2un:,vPt@p)_V>B}~fd6 Rc -"Br!G*Yak7g@YH,#N*6Vm4;~ XZ7,Od2-W =S:iqMTrR0o?"u7`v3J4{S\jP5 ^/)I@9p\QSsfcQ >7 $ s u/?{KL}7! h*-GpQ`D9 d:"/pAvO$ybjvwJWz^X-c.OVNca@xZP`>;E>Uo;Q(L[S`LRd}DLqtzYJgRoic;p ~fgrVwDP3EtIxb.BF|Z+46)?'a8J|;Ga~707LCk:Y!EQG@h;xG)3Iw) dj<nn~y{dEgMvw/  ,u / |Hzw0XF(VY2I yYVW3v@DG; YxqbZ_;8Hh ;Z qb'4+JgDAAGAGGCGCTCGTGGAGACAGGGCACCTTTACACACAAGCTTTATATAATAAATTTAATACAGACAACTACGACAAGACGCAAACCGCCAAAAGGGAGGGGGGCCTTGCCGCCTCGCGGGCTCTCCCCCTTTACAGGAGCGGGAGGGGTTGGGGGGAGAATAGAAAGGACCACAGCTCTTAGTTTTATGTGGAATGGTTTAGGCCACCGATTTTATTCAATGATTGGGGAGGTGCCCGCAGGATGGGACTTACATAAACGCCTTCTAACCTTCCACCTATTGTGAGGAAGTTGGGAGTTATTCCTTACTTGTGATTAAGACCTCATCCCATGTGAAAAGCCATGATTATTCTCCTATTTGGATCAGGTAAATAATAATGGAACCAAACCGATTGTTTCTGCTATTCCAATGGTACCGGAAGGTCAAACCAAGGAGAGGGGGCTCCTTATTGAAGATCTAAGACTGAAAGGCTTTTGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:10:04 -04:00AEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPtASPtB1PtB1PtBCTSGuBufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA 'O2DATA 'O24DATA 'O2DATA 'O2+DCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~ELIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOIS"eOfScxP1AM50P1RL1xP1WD8P2AM@XP2BADP2RL<PBAS$tPBAS"PCON/PCON-PDMFuPDMF"|PLOC*PLOC&PPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%"]SCAN3SMED SMLtSMPL]SPACAj*SPAC"uSPACAj*SVER4.0SVER GjSVER,,^Satdx^Scal@Scan3TUBEF8Tmpr<UserphARphCH"phDYbigphQLcphTRphTR2This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPtASPtB1PtB1PtBCTSGuBufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA 'O2DATA 'O24DATA 'O2DATA 'O2+DCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFTabUFVocUFWO_GATCFeat..UGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~ELIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOIS"eOfScxP1AM50P1RL1xP1WD8P2AM@XP2BADP2RL<PBAS$tPBAS"PCON/PCON-PDMFuPDMF"|PLOC*PLOC&PPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%"]SCAN3SMED SMLtSMPL]SPACAj*SPAC"uSPACAj*SVER4.0SVER GjSVER,,^Satdx^Scal@Scan3TUBEF8Tmpr<UserphARphCH"phDYbigphQLcphTRphTR}g#XrV?ybTio0<57#u?GG;Xj`:WnJuYSjMr[Vu`?+rh0v4"qp~!U#Mm[*&`cz"B``,#th2k0[+D:rF(5,fc-,RIlVinJilZbCd]kGZN"RgxwJ|*I*]E64Wg?UWU;j(6udrV|gwCiOfFc':Z^j?F?hECel7B]%j'kjWceOSx+C!K}Z'BNp{ e,&o.jrm$v5GK:.\_'[wA#Q9Tc T-*<^k'nwi5'S;.xhKn6g;X6YMI){wclQ[>A{%i/"a-cZ,f*F8B,}|O[nY?KPQh3f9beF4GAAsZKGrsy i3?b<|)=|=M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3<("Hf.Q+s3TtGK><3$G,lwd-l#frL?*Y^gM*yAEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPtASPtAUDT~~eB1PtB1PtBCTSGuBufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA 'O2DATA 'O24DATA 'O2DATA 'O2+DCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFTabUFVocUFWO_GATCFeat..UGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE6LAST~~ELIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOIS"eOfScxP1AM50P1RL1xP1WD8P2AM@XP2BADP2RL<PBAS$tPBAS"PCON/PCON-PDMFuPDMF"|PLOC*PLOC&PPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%"]SCAN3SMED SMLtSMPL]SPACAj*SPAC"uSPACAj*SVER4.0SVER GjSVER,,^Satdx^Scal@Scan3TUBEF8Tmpr<UserphARphCH"phDYbigphQLcphTRphTR