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"                                        "                                             !    !          $      %    %               !       d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 10f47291f1a0493fbe63ae96230b88afSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_E_9_Indeterminate_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7z*5BSk *MrCr1]"Z>Xjw~}viV<Z&J JqM.(AZr$/7:6+~a@y`J6% |wrnkihhhgea]YVTSTUTSPKGDCCCDEEFIMT\fr$Js:s -DNL@+g0O zxx{)d  wJ, 2 H W O"-YHt a;)2Z{S6# zhXI<0& /E]yN[3^yoLA:|bXVSH46\X c2Vd[<mTa$~{zzzz{{}~~|yungaZTNIEA?>?AIWrm-[/D89av+nZNHEEFHKMPRSSTVX\agnu}ym`TI>5.)&$$$$$$%'),04:AIQZcilkhcagx U>xo,~%hI8116>FMQQNHA:4/*'#  "$'*-159<>??=94-'!       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K V b n y   $ 0 ; G T _ l x    * 5 A M Z f r }  %0<HT_ly !-7COZgs &1=HT`m|   4  !/6%> ''">>>,;;+;;>;&>>;;;>>>>;;>;>!8;>>>>;;>>>>>>;;>>>>;>>;>>>>8>>>$ 1188>8>>>>>>>>>>>>>>>>>>>>>002$*+8;>;;>>>>>6>>>>>>>>>;8;;>;;88;>>>>>;88>+.2$&++3.(;3>>;>>;8;>>>88;;>>>>>6>>>>8;;;;1+.+1++11".(.&)",'(231)11..3120+2882'""3#".31180;'+-+32""-(828-2'"-3;;8&&-''--'"233+,''''      4  !/6%> ''">>>,;;+;;>;&>>;;;>>>>;;>;>!8;>>>>;;>>>>>>;;>>>>;>>;>>>>8>>>$ 1188>8>>>>>>>>>>>>>>>>>>>>>002$*+8;>;;>>>>>6>>>>>>>>>;8;;>;;88;>>>>>;88>+.2$&++3.(;3>>;>>;8;>>>88;;>>>>>6>>>>8;;;;1+.+1++11".(.&)",'(231)11..3120+2882'""3#".31180;'+-+32""-(828-2'"-3;;8&&-''--'"233+,''''   _GN?F%[N=H6 T1:F( E>$6dC_LG5Z`:TL70)=@R 5 HQ?#L$4 2 =I2A!<4**:_P*6J>K /',HY*b3_`H($Z;W2,`$aL5QA 8 -$:+?/RAK"Q-V%L#@@G&A L1Ac1$\[;6/[K;,*b (ICR":^ACN 1aLL7 \M6VTC,=L/?^\C&a:W9B'- I'_HLW#</+IT!7,VQTE ,T V .A;P'G/>;*/,NJ1Q1*D7b*\2*,.U)(+,%#\UUFQ_2C+,L\  <HU\, ";C] Uf`3- \NIs|E[;F+|FN\Y7d_^-Vt#3P&u0(cuYpM5auJS'}2zd[_[P%=!S\>&`=f  mF/>}jRb6[)h,yxR?]$'pr1$e9f _ Ge>=.bN+ Gj7Bgn)(8uuuNY3nA>@N @~+TQBd:&Es=K-5 HY} pQ.|]4<j -g}So[^fPJMMMxm@3M7:E$@ ,  'G 2&;dppWx,LSX)#NppvpP;@ ,r=wX DRy8 dpn&AaA{zr#Nn{DV O u AC\d:D_B= k$g\d#Bd4*&B#RFA+/ddgh`X"(E#4da&9axi{eXJLv~a9X47'kWW}S8|,C(m TDdSj:<Xj/uE+H53C\"!6)8_)B;\#6@gAD?2!"0,!/;0H=9.$QM@Iq;7M53@)4'(#&.-*!%3EsR@G3DRG%U 7s:>4U(,93A/ X{DyH|n5Jf35-]%'h)M<AN9#FfkQ+(lNyzdWZM]mI D 0sI'S{r@Cpsek'yrBNUviO\{g#4W_c AGAGGCGCTCTGGGGACCAAATCCCGTCGAACCTGCGAAATTATACTATAAATATGTAATAATTTTAGCTATAGGGGGTTGGCCCTTTAAAGGAAAAGATGGAGCATAGGCAACCCACTTGGTCCCCCTTGGAGGTGGGCGTTTCCTCCTTTTTTTGGGATTTACTATTCCTATTTTGGGAAGCGCTCGCGACTAACTCGTGCTCCCACGAGAATGGCCATAGTTCTTCACGCCAAGACTCGGGCTCATACACACTATGACAACGGTCTCATCTATAGGTAGATGAAGCTCCTACAGCGCCTAGATCTAGAGAAAGGTAATGAGCATAATTTGCTTGAGGCCCAAAAACAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:10:20 -04:00AEPt3AEPt3APFN TAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS=^BufT"DCTIDܫCTNMܱCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DRDATA"DDATA"DDATA 'O ]DATA 'O 4iDATA 'O uDATA 'O ҁDCHTDSamDySN µDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFWO_GATCGTyp!!”HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~;LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS!OfScXP1AM_/~P1RL_,P1WD_2<P2AM_7P2BA__:vP2RL_4PBAS__#'PBAS__!PCON__+aPCON__*PDMF=PDMF!PLOC_'DPLOC_$PSZE`PTYPPXLBRGNmܣRMXV1RMdN ԍRMdV1RMdXԖRPrNqRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate ˆRunN""܁S/N%!SCAN3SMED SMLtSMPL SPACMSPAC!SPACMSVER4.0SVER =SSVER,,SatdX^Scal@Scan3TUBEE9Tmpr<UserphARphCH!phDYbigphQLcphTRphTR*UThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN TAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS=^BufT"DCTIDܫCTNMܱCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DRDATA"DDATA"DDATA 'O ]DATA 'O 4iDATA 'O uDATA 'O ҁDCHTDSamDySN µDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFTabKFVocKFWO_GATCFeat//KyGTyp!!”HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~;LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS!OfScXP1AM_/~P1RL_,P1WD_2<P2AM_7P2BA__:vP2RL_4PBAS__#'PBAS__!PCON__+aPCON__*PDMF=PDMF!PLOC_'DPLOC_$PSZE`PTYPPXLBRGNmܣRMXV1RMdN ԍRMdV1RMdXԖRPrNqRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate ˆRunN""܁S/N%!SCAN3SMED SMLtSMPL SPACMSPAC!SPACMSVER4.0SVER =SSVER,,SatdX^Scal@Scan3TUBEE9Tmpr<UserphARphCH!phDYbigphQLcphTRphTR)=8m|4'v;L`-6Lrj~;b3?8r**5{H\V'+io.qmawA.&P`O_'?Z2ZR0Xwtej_@qW8SWXM=4T*RG_C26R&w^/~!Kp8&O"tX {1tp2s7}Z&[VGkB*Sig]r;a4xG57'!>&7.{M}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@tt/rpi)3c+F@9$AKV=Y~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MK ?"$@QzOlJ,.|]@Vr,u!&.z*Rr,Z:X=<;s_|g+VFl@i2go~WR&^.9,(B~bHjAt3_iP""#]eRWJT|eiB]%j'k0WC.%ouO{9X6!7&o.ja8G$l=b d[qm#vRRC(vJnPX=<-8 )S-F3x@M9X`|W"[XYTZM%SmvlM@=K}VX*os7}Z&[Eb4;,#|>WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN TAPXV2APrN APrV1APrX}}ARTNASPFASPtASPtAUDT[B1PtB1PtBCTS=^BufT"DCTIDܫCTNMܱCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"DRDATA"DDATA"DDATA 'O ]DATA 'O 4iDATA 'O uDATA 'O ҁDCHTDSamDySN µDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFTabKFVocKFWO_GATCFeat//KyGTyp!!”HCFGCEHCFGHCFGHCFG==InScInVtLANEGLAST~~;LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS!OfScXP1AM_/~P1RL_,P1WD_2<P2AM_7P2BA__:vP2RL_4PBAS__#'PBAS__!PCON__+aPCON__*PDMF=PDMF!PLOC_'DPLOC_$PSZE`PTYPPXLBRGNmܣRMXV1RMdN ԍRMdV1RMdXԖRPrNqRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate ˆRunN""܁S/N%!SCAN3SMED SMLtSMPL SPACMSPAC!SPACMSVER4.0SVER =SSVER,,SatdX^Scal@Scan3TUBEE9Tmpr<UserphARphCH!phDYbigphQLcphTRphTR