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d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 3}{xwtrpn P P P H H H e f f } } } (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}| < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ffb097b65ccd4451b751393dfa03d625Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-04-14_06-39_1995 3130Seq 10143 10143_Lehman_K1361_trnh jmadera 96-Well K1361_E_7_Pinus_chiapensis_trnhKB_3730_POP7_BDTv3.mobSeqPop7z 1Nm3Z UCsN /<GNOK>*p>=s;~U0.IbzpO/qR6 x p h ` Y R M I F E C A > ; 8 6 5 6 7 7 6 3 . ) % " ! $ ( . 4 : @ G O [ j Hu =j]-b2 { t p o s { LTF
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SA A#aAaA &KB.bcpKB_3730_POP7_BDTv3.mobtermAGTCGCGCTTGCAACTTGGAGTCTGGCTCATCGCCGGAACAAGCCAATTTATCTATCTACAGTCATTTCTCCCAAGAAGAAAAAACGAGCTAACGTTTGTTCGTATGTGGGTTGGTGACTCTGATAGGGGATCAAAGCAAGATCGATGACTAACTCCCAACTCTCGAACAAGTTGTTCGGCTTATTATAAAAATGAAATGTTATTTGAATGTCTATTGATAATATTTGACATGAATCAATTATGAGAGAAAGAATGTAAATGGGTCCCGATCCCGAACTAACCCATTTTAGATCTGAGTCTATACTCATATTATAGAATGAGTATGTTGATGATCTTTATCCAGCATCCATGGCTGAATGGTTAAAGCGCCCAACTCATAATTGGCGAACTCGCGGGTTCAATTCCTGCTGGATGCAGGGGAACTGTTTTATATTTCATTATTGATGGAATGGGGAAGAAGTCGCGCTTGCAACTTGGAGTCTGGCTCATCGCCGGAACAAGCCAATTTATCTATCTACAGTCATTTCTCCCAAGAAGAAAAAACGAGCTAACGTTTGTTCGTATGTGGGTTGGTGACTCTGATAGGGGATCAAAGCAAGATCGATGACTAACTCCCAACTCTCGAACAAGTTGTTCGGCTTATTATAAAAATGAAATGTTATTTGAATGTCTATTGATAATATTTGACATGAATCAATTATGAGAGAAAGAATGTAAATGGGTCCCGATCCCGAACTAACCCATTTTAGATCTGAGTCTATACTCATATTATAGAATGAGTATGTTGATGATCTTTATCCAGCATCCATGGCTGAATGGTTAAAGCGCCCAACTCATAATTGGCGAACTCGCGGGTTCAATTCCTGCTGGATGCAGGGGAACTGTTTTATATTTCATTATTGATGGAATGGGGAAGA . K X a w "/<IV]n )DO]n )6@LXeoy (4BMXeoz *7BO\fs +4AMZet %0;IT`ly *7CQ\hu * 6 A L Y c o y
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i ] F Q P j e a ` g %Q)p g ; j * F 4 ? D, GAGGCGCTCGTGGGACAAACTCTGTTAAACCTCATTTCTACTTAACTAAATAGAGATATTCAGTTGAATATTTCCCCCCTTTTTCACGTGCTTTCGAATACTCACACCCCCGCCAACCACTCATAGACTATAATTTAATCATATATATCACGTTCTTTTTAATCTCGCACCAGCTGATCATCATAGTGCGGGGCCCCCCCAAGAAAATGGTCTCGAAAGGCCACCGGAGAGCTTTGGGCCCCAATATTTTTTTTCCATCTGTTAGTAATGGGGACCCAGTCTTGGAAAAGCCCAATTTGGATCGGGGGCCAGCCCCCTAAGCAGACAGCCATGGTAGATCGGTTTGGTTGCCAAACGGATAACTTTCGCTATCCTCGCGCTAAATGCTTGGGAAAATAATTCAAAAGCTATATACGCATATTTTATCCACTGTACCTTACCCCCCACAGTGTTCTGGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-04-14 08:10:05 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS LR BufT " D ! CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D V DATA " D DATA " D DATA " D " DATA ([ P DATA
([ P 6[ DATA ([ P DATA ([ P DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT ª EVNT ½ FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 7 LAST ~ ~ J LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS ( OfSc | P1AM : P1RL 7 P1WD >< P2AM Eh P2BA H P2RL A PBAS * PBAS ( PCON 5E PCON 3z PDMF PDMF ( PLOC / PLOC ,N PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ܹ RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT RUNT * RUNT Rate RunN " " S/N% (} SCAN 3 SMED
SMLt SMPL e SPAC Aj SPAC ( SPAC Aj SVER 4.0 SVER LG SVER , , f Satd | ^ Scal @ Scan 3 TUBE E7 Tmpr < User phAR phCH ( phDY big phQL c phTR phTR )ˁ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN O APrV 1 APrX } } X ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS LR BufT " D ! CTID CTNM CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D V DATA " D DATA " D DATA " D " DATA ([ P DATA
([ P 6[ DATA ([ P DATA ([ P DCHT DSam DySN Dye# DyeN DyeN
DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT ª EVNT ½ FTab Z FVoc Z FWO_ GATC Feat / / Zm GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 7 LAST ~ ~ J LIMS ! ! % LNTD 2 LsrP a MCHN MODF F MODL 3730 NAVG NLNE ` NOIS ( OfSc | P1AM : P1RL 7 P1WD >< P2AM Eh P2BA H P2RL A PBAS * PBAS ( PCON 5E PCON 3z PDMF PDMF ( PLOC / PLOC ,N PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ܹ RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT RUNT * RUNT Rate RunN " " S/N% (} SCAN 3 SMED
SMLt SMPL e SPAC Aj SPAC ( SPAC Aj SVER 4.0 SVER LG SVER , , f Satd | ^ Scal @ Scan 3 TUBE E7 Tmpr < User phAR phCH ( phDY big phQL c phTR phTR k&zt Mi3rs{6`V5r@AKl&FaewMZ>fcn.=[|ebhktH4*&qve4q,8kf RCjxeCW?R5Ch2d'jw~|>J3zFReL#(78WFoe~8/zz-nX>z3KQv3.5s\yC!}WQ`Q5f`lbkF3(jY`VqQIxwcL6-*pfFUeiM/+U*yxT/4f!Z56N'?k?~pmxRVQQ$zWkp$=RyS4*7rtlq8&LabDvv'O` JECce3YXjld96"$M@>b|U2mC!KCo6T*-dotq$C9|f|Qc^F"P95Li2R~5OPh~jThh@<}9W#()P0&Mqzva].p>x'[vA-\K"L1{!Ul62dl=?o'%:`Kle8h/_Dea_';_/d@ikjFDfqcd o;X.>S[jWcgnW6Ob#7!IkW\tbrq$^j#_FJg)+^@|DzUC`k@l'Z9dgTiK]j;K.LzeDW[Zeh&f&{-'_1k=`<{!s73|?j~J!Eb4;,*`S]8a,D:rF(5,fjG,F<_JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"+%3NPUBD6P$ f*BEQ6x}_og|T]p oL]3Fk+J{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`Vcy[*oCf$`6A&03d02R P,hzp!tY`< P2AM Eh P2BA H P2RL A PBAS * PBAS ( PCON 5E PCON 3z PDMF PDMF ( PLOC / PLOC ,N PSZE ` PTYP PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN ܹ RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT RUNT * RUNT Rate RunN " " S/N% (} SCAN 3 SMED
SMLt SMPL e SPAC Aj SPAC ( SPAC Aj SVER 4.0 SVER LG SVER , , f Satd | ^ Scal @ Scan 3 TUBE E7 Tmpr < User phAR phCH ( phDY big phQL c phTR phTR