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'  "#         !  $        &#4EPJC6J2,.+*8*EP30;7" *,-/2.+HLC79CH2%!+!(+=S/ " '$           !"#$%&'()   !"#$%&'()d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9ec48dac9ea240f497692186ac6c4b5aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-Well!K1361_E_11_Brassia_verrucosa_trnhKB_3730_POP7_BDTv3.mobSeqPop7z#2@O`s!<[~-Y5b;db?n?l5u\C,%1>N_ty_A!qZE2!}zvpjd`^^`acca^ZWSQOMLLLKKJIIJKMPTX^fp|EvQ;pW"aj: /`:A j G  i & 6  + :Urb$m\OB6)5^1BrG%  \ R  W v  o .*H-pZ?qCwpjgffip{BdGY Ltl=E7iTG=4(}wsrtx~paTIA:4.("$,5AN]m~yeQ?/$    &0:DKOOKC8,    "/;DKNNJB91++3D]zdG/ !.<JU[\VJ;* #7PirV:!       %,4;?@>91'    ',,)# ,:FLKE;-  &.341) '29:5+  "')'! .AR]`\O?, $2<BA;/"  %,254/(  !&('"  '=Sfrtl[E/&0672)   &/6971)    1Mg|t]C*  *6?B?4% 1BNSOD5%   )--(  #(*("  #.6:70&#6GTXSE3  $.560&          ##$(1?P_hi_O:&   $+059<<93)     !,585." $5CJI>.  )8AC=1"       )154-" &.33.& &/562) !! !(*'      ""+9BEB8+   +5:7.!#()$  !$# "2>CB;/#     "$#       &.21+! &-.)                  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"{@A"(AOA\KB.bcpKB_3730_POP7_BDTv3.mobtermGATGTGCGCATTCGACACTAGTATGTTATGCATACATCCGCCCTTCTTCTAGCTTACGAGATTCTTTTGTATTCCTTCTTCTTTTTTTCAATTATTAGAAATTTTTTGTCCACTTCTAGCATACAATAAAAAAGAATTAAAACATTCATTTCTCATTTTTTTTTCATTCAAAAAAAATCCTTTTGTTGCTAAGGATTTATCGGGAAATTTGATTAACTCAACAGGAGGGAGGAGAGAAACTCCCATATGTTTTTCCCTCGGTCAAATACCATTATACCCACAATGATTGGCCATACAATCGCTATTCATAATGGAAAGGAACATTTACCTATTTATTTCACCTATCGTATGGACGGTCAAAAATTTATTGAATCTGCACCTACTATAACTTTCGTGAGACACGAGAGAAACGATAATAAATCTCGTCGTTAGCCGTTCTACAAATATGCATGTGAAGAGCCTTATCTTATAGGGTTTAGATGGCTAACGATGTGCGCATTCGACACTAGTATGTTATGCATACATCCGCCCTTCTTCTAGCTTACGAGATTCTTTTGTATTCCTTCTTCTTTTTTTCAATTATTAGAAATTTTTTGTCCACTTCTAGCATACAATAAAAAAGAATTAAAACATTCATTTCTCATTTTTTTTTCATTCAAAAAAAATCCTTTTGTTGCTAAGGATTTATCGGGAAATTTGATTAACTCAACAGGAGGGAGGAGAGAAACTCCCATATGTTTTTCCCTCGGTCAAATACCATTATACCCACAATGATTGGCCATACAATCGCTATTCATAATGGAAAGGAACATTTACCTATTTATTTCACCTATCGTATGGACGGTCAAAAATTTATTGAATCTGCACCTACTATAACTTTCGTGAGACACGAGAGAAACGATAATAAATCTCGTCGTTAGCCGTTCTACAAATATGCATGTGAAGAGCCTTATCTTATAGGGTTTAGATGGCTAAC6Wg"1=HRWbky )4BO`q!*5K[fw",>GT`my #.;ITblz (3AKVcp| %3@IUamz(5BN[gt    ) 5 A O \ i u    ' 4 ? L Y g v  # - 8 F P \ j v  - 9 D R \ h s ~  ' 1 < H S _ j v  &1?IUanz+7AMYdp| !,8EP\it%/<HT_ly (5=HTamx(0<LXboz'3?IU`lx(7@K\ly"/9IQ`js !.9ER_hr6Wg"1=HRWbky )4BO`q!*5K[fw",>GT`my #.;ITblz (3AKVcp| %3@IUamz(5BN[gt    ) 5 A O \ i u    ' 4 ? L Y g v  # - 8 F P \ j v  - 9 D R \ h s ~  ' 1 < H S _ j v  &1?IUanz+7AMYdp| !,8EP\it%/<HT_ly (5=HTamx(0<LXboz'3?IU`lx(7@K\ly"/9IQ`js !.9ER_hr   #'                "        ""             '  "             "(12 """   #  # $                                  #'                "        ""             '  "             "(12 """   #  # $                               I2'@)YEQ(Kb] &'7!P4V& 3U* Y,*O>GJ6 [ZA2..c*I ? TKa'8TB3JU&># Rc"bTB:"8-ZFD DJ8NK8VAU5`TC0VT9I B&cJVV0=.6ccICNOS *'KSE^ H!<9DRK;I BR2J V)9LF!#a]>ZM>;%$DED/%=8 LR2``$ a NS!<6 W)S (F _CP<*3[6.!DDTZD'!$_T=QHb(7!S(b$M9J+'E ?"=^aC0 BXXb-F ?)M=@RHX"5>8H$2*=P74,5aYT,QM 6'D 6S+J76PPN53`5O&0G/$2 OC-SB]= bBNZJ9?[X <Q`d K0#V"M\H=*dS_6$$AW  !7 +]:K%'3'"84 BI\MH& &` UUO-4>" MSR)= /E HLMD!P  i|>[ l~!(sa&js q 0*WQD/^!W.,we*wPETA9  g;Xvi8Eu"   }0^5C$}QL|PnV#'M%on* == yCJZM"3d{p$pH5? r lh s{Y6y}4,~NX;e&t )y)zrv1n)<fxF wx>VNE#;IF}5\LHr`pB *q?@ G!"4aofB "/j#2l{ewJnh?kJ{eM$  1 b#X >Z{ ` `b5 ;F%>}bs?p$G\?38<7  /)!(2 C* ='!=^UMT$4`/(=9^JSk "9, #Wn\  :  !#'#  >N W +i;2<?4 Bq@OBlR2&@Bu)( Ch ] #B%hcf>:/X!,RpLKbGEn4JxafdR+bZO,hRxq/A"I]7,=Zp )K5FhDi{Lp&8rK]z$]0| 9 9cRT Mb0iSO;zID"L$Lb>tSuf,+&1eiJ?v"*y nz8FX p,#hqShpE-BBMa6 '8WB}8C,?AY("#QP>Y,i~s{LF58-8]&q55}d =&C0OgWi.`eQse+5s><;!arj:o(*IV ipcCt{QE"}Aq)*7RD.SuzI;df.1UD( /t|X~XM &IX^;^P y|59-8lgs[<87& ^mx!vpAwi5M|dU!L'G!px/vrZrwjqp4"z_vi-b> PStKt/G P\@OzoWMlb C L |7v2 E6d4t=rKfN[AGGAGCGCGCGGGAGACTCCAACAACCTGTACACACAAGCGATACAAATATAAAATAATTTCCTAAAATCCCCTTACTCCTACACAACAGTAATAGTTCGGGACAAACGAGTTAGAATAGCCCTTTAGGGCTTTTTAATGGTGGAGTCACAGCACGGGAACGAAATCCTCCCTGGGTAAAAAAGAAATGCTGTCGAAACCGTAATGGAAAATAAGTAAACCAGAACCAAGATTATAGGGACTTATAGATACCGGGTTATCTGTTTCCCATGGGGACAAATGTTGAGAAAAAATCCAGTATTTGGGATGGGGCTACCCAACGGTAAAGGAGCGAAGAAAACTAGCCTTATATATAAAGACCTTTAAGGGAAGTCTCTTTAGGTTACAGCTCCGTTGACTTTCACGCCACGGTTTATTCCCCATCAAAATGGTTTTTCCTCCTCGCGTGTGCCAAAGGACAGGCCCCACCTATTTAGGGGAGCACAATCABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:10:46 -04:00AEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPtAASPtAB1Pt@B1Pt@BCTS7sBufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA %{JDATA %{J0DATA %{J{DATA %{JƁDCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~5LIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOISOfScxP1AM$P1RL P1WD(P2AM02P2BA4P2RL,bPBASPBASPCON PCON"PDMFPDMFPLOCRPLOCPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%wSCAN3SMED SMLtSMPL""]SPACAo+ SPACSPACAo+ SVER4.0SVER 7hSVER,,^Satdx^Scal@Scan3TUBEE11Tmpr<UserphARphCHphDYbigphQLcphTRphTR/FThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPtAASPtAB1Pt@B1Pt@BCTS7sBufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA %{JDATA %{J0DATA %{J{DATA %{JƁDCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFTabEFVocEFWO_GATCFeat00EGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~5LIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOISOfScxP1AM$P1RL P1WD(P2AM02P2BA4P2RL,bPBASPBASPCON PCON"PDMFPDMFPLOCRPLOCPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%wSCAN3SMED SMLtSMPL""]SPACAo+ SPACSPACAo+ SVER4.0SVER 7hSVER,,^Satdx^Scal@Scan3TUBEE11Tmpr<UserphARphCHphDYbigphQLcphTRphTRU[lWQXb^kOptpuD:\E+mHY`/&mayX9'-vyNJRA=57AMjCW`D/z\|DX&_lp2]m"]P"F77sI_6sKGol83p{ ]GxNu~)B*HUrG\W\lYT`M=4T*R7R8~f)NK[>Id`Po<_+MWQ}VX*oE}`Z&oRLV;);-ags5)9mW'>YP~jhM^,%bkb |{cokB}qHqHStXK#XTE-=[c}Sn2M5FiK&=RSbXW,62DmOYX/D?**s$[-p+D42F7f6QuA!9@1%ilJG82G [7P^E$62:gtQ]uYQfaX+/kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.EW<#G@njH[=K{_"#k6l2WeH?WhjH@(Te#:MGG;16#YGr&kWgw/|9vAMV^@Yo]p&qn~+lU,YTl_{1#p{XPTF}cyy3of)`|!-Vt2`\wmj773Ku\@\=5NDzU4oD7N**s$[-33.BkS7> YlGcRb[xy8J9tG>udrV=y`6@WBktK3c*6?FYT7~U+U*rR_"qaf/h7kZ5UPh_,zi8|6Ig,1cWs~jo~e7#+hc>b.I3u\oi$u1A!O3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03SMODL3730NAVGNLNE`NOISOfScxP1AM$P1RL P1WD(P2AM02P2BA4P2RL,bPBASPBASPCON PCON"PDMFPDMFPLOCRPLOCPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%wSCAN3SMED SMLtSMPL""]SPACAo+ SPACSPACAo+ SVER4.0SVER 7hSVER,,^Satdx^Scal@Scan3TUBEE11Tmpr<UserphARphCHphDYbigphQLcphTRphTR