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'  + "!("  %#+",$% &#.#,! #' 3%&  & -#"-' ' &%#$d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 802576b9439442c19e472a745c5e0fdeSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-Well"K1361_D_5_Nicandra_physalodes_trnhKB_3730_POP7_BDTv3.mobSeqPop7z3H^y7b4tVWL7B - d O 5~Cqhk< 'Fj<bvS)OwIq[KB>>ADGIJNT^iuwj]N?1"~{xwvurojd^ZWVUUSOKFBACGKMMJFCBELU`lx 5fJ BV[O5KsDQp \ XSTD-+9D d PA }S,jUB1$$/=L]t d4h9]i^:CJg$,l-< Vd&J1d t1~{zz|~}{yxwvsoh`WPJGDB?<9:@Qn"n {JZ/ 2J^dW3J*y[H;40..01222247=DLU^fnuz}~{uk_RE8,# -Jvc1nDrP=41246651,& #)/453/("      EX5M|m-XA"tL:68::986553/*$   #+1440*# GjK;Nv33sB#lq-YN$      "$"PQql3  ##!  #&&#    E|Z  %0:?>7-!   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OF>jKcg    Ez B  -~DP"E "" V<   ;          "&'%  "# Y7 >        1<.F(nO qb0q 4~&m ,vW1/6ln    #"                 6>(3!#Qe .oNg"g>*3vMv=C2L  "-55+]A #y06"Y/a2H   ,q"cz`m+    0eJ   a"w]K  <G:z/#nJQ  VP{vDK    A^+e"     3I3M `y./659&uM0o) J\k]  FYv=:)2BWFjC  'Y  [(    ,lQhPl- T0SIj*(f),A   (Xr>    7lo: Je.  :nuAI~q>"%FzzF 2ci;   6fO JK"      !L_0)Tf6  ,UsC  #*.-( =k_6 &)($      !""#%')+-./01356899:<>@BCCBBABBCDDCCBAAAA@@><:86433221/-+)'%$"!     !$).39?FLS[cmy &.6;@DHMSZahmqrrpnlllnruy~|hVH<3+&##)5Hc2d*SpuY1M Gvld^XURQPPPPPPONLIGFFFFFFEFFGHIIHEB>;9766541.+(&&'+/5=DLSZbjt~zqib\WRPNMLLMNPU]iz K[<+~Z2 D hvMTe f M ]Lw>R/_|qG eKEc? yqnqy<gi\NwvIFSMSB ~ytpmkhea^_fv1xWQOJMx`j ai}vv~%iUuox  1fLN ~`a7[f]K;8@Q`f`RB9=NdxwdPFPnvl##unieb^\Z[aq$,[) {;0VtvO vQ@:9:;;=BHQZbghd\OA2$ $&%  Duj2{1       6-p>F'&2>DB;4/-,+*(%! * z"j3{d!#$%%#    (BfQI !$%#  S\ 'Xnq)Eazz^<:) { ^ Pn+$/430-,,,+(#         ${e8%lV      Nx;JI0lC6U1  #!      !#!   "&('#     &,3:AJS]ht  rdVJ?6.(##,6=:,myfa\ )*'$""#"   SB-U5R]('"    kr[vGLT '&!        &un.5 :    =J.78HQ6J-V '("  qxSCH !   V5 {9   II   I2;:S    GNe`!       C 9_")}SSylAp{?,G "+*"   </Tt5!2AGF?978;>??>>@CHMONKGDEHKI>, M3e'     Ow<r+ BknF   E!%K  U= ;   !ao\P   ES4y4     !c+d<$L};~<      -e&Y)    L5cgBe/   $SO&    )ZR'      Nt>   *Q}W2   @qwI%    >nwN.    (M{W3 ':JTWPC2"   "T1)S3*1APXWN?.(;M[a^Q@, +4860&   7  @AAQ3AKB.bcpKB_3730_POP7_BDTv3.mobtermAAAGTGCGCTTCGCAACATTCTCGACTTGGCTCTCCGCCCCCTACCTAAATTCGGAATTTATTTCTAAGTTCAGTATTCAGAACTTGCCTTTGATTAAGTCTTGAATCCATTTTTTGAATTAAAAAAAAAACTGACTAGTACTAAATAAAGGAGCAATAACCAATTTCTTGACAAAACAAGAAAACGGCATTGCTCCTTTACTTTCAATAACTCATCTATACTAAACTAAAAACGAAATTTTATCCATTTGTAGATGGAGCTTCGATAGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCCGGAAAAGTGCGCTTCGCAACATTCTCGACTTGGCTCTCCGCCCCCTACCTAAATTCGGAATTTATTTCTAAGTTCAGTATTCAGAACTTGCCTTTGATTAAGTCTTGAATCCATTTTTTGAATTAAAAAAAAAACTGACTAGTACTAAATAAAGGAGCAATAACCAATTTCTTGACAAAACAAGAAAACGGCATTGCTCCTTTACTTTCAATAACTCATCTATACTAAACTAAAAACGAAATTTTATCCATTTGTAGATGGAGCTTCGATAGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCCGGA -E]s "(1<BObr}#,5CLT_kt~  -9DMXdp| #/;GS_kv)2=JVbny%0=JT`jv  *5@KValx  +5@LXcny   % / 9 E P \ g p z  ! , 7 B M X c n y    ( 3 ? K W b l x    * 4 @ K V a l w    & 3 > J T _ i u $2>JU -E]s "(1<BObr}#,5CLT_kt~  -9DMXdp| #/;GS_kv)2=JVbny%0=JT`jv  *5@KValx  +5@LXcny   % / 9 E P \ g p z  ! , 7 B M X c n y    ( 3 ? K W b l x    * 4 @ K V a l w    & 3 > J T _ i u $2>JU    -.4".!1  >>>/&>'8,>>>+8;>>>,+>>>;>;1>>>"021*61;>;>>>>>>;>>>;1>>>>>>>>>>;>>>""";86666;>>>>>>>>6>>>6>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>;>>>>>>>>>>>>>8;;61+688>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;;>>>>>;>>>>>>>>>>>;>>>6+;;>;>>>>;>>;;;>>3'1       -.4".!1  >>>/&>'8,>>>+8;>>>,+>>>;>;1>>>"021*61;>;>>>>>>;>>>;1>>>>>>>>>>;>>>""";86666;>>>>>>>>6>>>6>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>;>>>>>>>>>>>>>8;;61+688>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;;>>>>>;>>>>>>>>>>>;>>>6+;;>;>>>>;>>;;;>>3'1   H(!#PB/W0E7 +*B A)X^@TXI"RT9Y >EU;,D 93#OD)L<!c" NW 28 &UYR[ 2=P. 1F(J+8$9G8OU&<-O ZB2WI=" K! D  5> ])P`S` &8.PL% 8'C;@)cH./!M&?,#.IC/ DNYT0#_)APQG9=*='P`RRX.$:J6!dF(37]*EQL@3IFS;T7]P('M%71G*8MQ) Y\ T Q$!A*0Q%'P H)_M1DG<H0I W#E;^C5/+[*B* x[SC: Tg. "}|n9 JR4Y8\="OQh$?S$>`/)Rw'/ M/_:Q LK:YYc+?lwSC.Kb,@kn2Q_%c@ 1}Fi,D};@Ftkvd*9hguQw43DS_nrKe4"S'LR^O@).*]UF)8^?n]UtP(+  0G%)0xn9@drx@V@D=6@Tx2,'8#=.= 6    -~-apqpgJdpdv~?: p/O5('Z8_ RPb5S|p:9Ab,*$G Wjd,?DnvYr.MELtvBB7 {51p ,pw%#O,$Cs,S8%-^{h`2$8\[b8}B%)/+-Ll6`vsW&-&/"J.%-'Fl>{u *''[T ; +. \h}WMdU.  ( I"'T5 - - ((*&)<(OkY]_pO;D)#"&<*!'*0,0;& .05&.+&"=&  /<--G%St'<>3-!0 +,#- $3!3"."I7L$&.4%939E&6%.: -!"F##0.,&8;#).)%|X">7,=I'52)!hF@)$Rho;~)bjGGGAGCGCGCCGTGGGACCCAAATCACGTCGAACTGCGAAGTACATATTTAAATAGTAAATAACTATGTGCACCATAAACCGCGCCCTTAAAATAATGAGTCCCGGAGACCGCCGGTGGCCTGGCCCCCTTAAAGAATTGCTCTTTGCTGCTGTTTTATGTTCTAGCTCGAGATTTTTCGAGTTCACAGTAGCGCTTCAATTCCCTCTAGGACGCATCTACTCGGTACTGGGCTCGGTAACATATTCCGGTGTAGGAAGAGCCTCGAGATGCTCCCAATCGAGACAGGAAGTAAGAACCATTCCGTTCATGCCTAAAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:09:51 -04:00AEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSBufT"D!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DVDATA"DDATA"DDATA"D"DATA #GDATA #G-DATA #Gu`DATA #G<DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ’EVNT žEVNTªEVNT½FWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS OfSc|P1AM@P1RL@SP1WD@SP2AM@SP2BA@@P2RL@PBAS@@PBAS@@SPCON@@PCON@@ PDMFPDMF7PLOC@ SPLOC@PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܹRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPL##eSPACMSPAC0SPACMSVER4.0SVER SVER,,fSatd|^Scal@Scan3TUBED5Tmpr<UserphARphCHNphDYbigphQLcphTRphTR28This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN OAPrV1APrX}}XARTNASPFASPtASPtB1PtB1PtBCTSBufT"D!CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DVDATA"DDATA"DDATA"D"DATA #GDATA #G-DATA #Gu`DATA #G<DCHTDSamDySN Dye#DyeNDyeN DyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ’EVNT žEVNTªEVNT½FTab,FVoc,FWO_GATCFeat//,GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~LIMS!!%LNTD2LsrPaMCHNMODF FMODL3730NAVGNLNE`NOIS OfSc|P1AM@P1RL@SP1WD@SP2AM@SP2BA@@P2RL@PBAS@@PBAS@@SPCON@@PCON@@ PDMFPDMF7PLOC@ SPLOC@PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܹRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPL##eSPACMSPAC0SPACMSVER4.0SVER SVER,,fSatd|^Scal@Scan3TUBED5Tmpr<UserphARphCHNphDYbigphQLcphTRphTRMr,c],~u:L< zFefcXM7WX}lna] 5EX6R2? Cq4GPQFG&908]/eJ!`WBV*O.:U q<6(mu y-WPv8UUCEDFi3V[,A4#OvRio5z{,'~E{}P.Ea}_eaejp~0,{VBtn&gqr;zuhGlB5-S6C,nJ3qo8Mew.KzQ&:C=oq#g%rl(Lii$sL9,X/5`w=yBimJEIoM/V,<|ag&<{[;N }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[Xd:< dG#$w17h+eJ/n=Y?iQ7wDUfS*rR_V?7ZXmIcC+D\z6 H,L?+VzX=aplMUo9=T?$mInJ?$OA2nrD5pYlGcRb[q5FaT9Vbr$rv/qNE^O{|cHT?Ou4|8e7spFp[O6h"4#O|!f|bEdG;_{8@hh/WZ#3|)l>,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*c{Xm|3HF)SE8n\qolG&uYZ1b6 +l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.