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" "+$'$#' &! #  "0@c{b=IQqcQ='+#)#"%   8-A.7'$944/(( $ $# '!"  #L1uq5 $14B<Q xmMNH08#"$#"   -ic<k0MeUP<=>+-%$    16eq\}&@hCsaXJW8L9IG;9L+#7E/2]yKH lrzdnJ.-).&  8Cm:GjvZ;eQ87,-$   "1@Ym2sk`@IC2>#$: )*$:,2.    /Ig?;9q6zwOG=A4%            %h! yDog]E0'$   &  &,FO1d"B]_.5/.+) 166819-/7+&609HeE`O[@tsXTNRUCQNAI;O-+,;^GczidcSen.'x}<~EyTcTqJ^eU[TLCDC9PG|,?x`5kU\oM=I<<BHT3fgpI7"t;t^GN9AIC(#lrajXfiy$J6 H jbA?<< #'+-"    0 ! # " (1&&3?/,8),%+#+,%5;,:)37307.*(. 0%"*"%0 %&                                                                                 !                                                                                      # $     (                   &       d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d2ac648865ff47769e6b4a72b24e438cSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_C_4_Urera_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7z  1?L]s;c%\KZM3v6t    {@I-{%vu.q`US]s=^ ])\.oQ7" %/7;;:62-'~wqkfb``abb_[VRNMLMMLKIGFGILOSVY\_dks~!Jz1x T,.!q/YuO3 &=`$ bCL} J f^A*  m x2/qjjC#tS5-JpE%wLoyW5D&;yWjNyX$d4^ ClYzohb^\[\^`abbba`^\XTQNLJHFC@=;::;>ERgF!,#}y,:%0*E=aG6,%" !"$%'),/49?GOX`hmpojbVI</%"'-5<DLRVWUROPXnq+{iStM7-,-/0/-)%!!%(+,,*(%"       (^d~tE) )18;;71*# <}L5X{ P"x1<  ",6<>;3*! ?I11F?  #%#   !#"   %3>EGB6#^SnQ1P  $*,*$   +3760(   !%&%!     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C4OJv6O mss^%EnM#&'(*,--*&     #+5?JS]gq|yj\PF?:62/*&     Fb$ZMSE "&%"!$)--( %} md`p),)%!        ?P $Aw,   "zd)3"$#!hU/ SE            "',27=BGJMPU]gt|vrolhd^XQKD>82,'"       !         #tziie       ZL/Q.Og%            c=Vz.    !`"~8t/    0zH!]   1[7e#    NDx\m/    F7=X%      !"##$$%%&&&&%$##"""!      /aP&  H -*t;'>{LW9h1   H|V+ 1][25fm;  #Q`2 6lJ! =uq; Bl^7 ,UpAApqI(   5Snv^A&7_nH( (Jo~Z8 #=Yp}{kS8  !?boO0  0PquW7  /G\hi`N9%  8SkyznX?(   6RjwxlV>)  "')($ 6Pgv{ufR>,  )9HSUPE5%  'JqoN5'$(/34/(   "6K]hjbQ=*#%#     "@AUAEAKB.bcpKB_3730_POP7_BDTv3.mobtermGAGTCGCGCTCGCAAATGTCGAATTGGCTCATCCGCCCTATACTATATAACAGTATTAGGAAAATAGACTCTAATTTATAGTAATTAATACTTCAATTTACAATACAACGTTAATAAGAATTTTTATTTTATTTTGTAACATAAACAAATTATTATTATTAACGAAAAGGAGCAATACTTACGTTTTTCTGTCAAGAATGTAAGTATTGCTCCTTTATTTTTCAAAAACTCATATACACTAATCTAATATAGCGGTATTATCCATTTGTAGATAAGACTTCTACAGCAGCTAAGTCTAGAGGAAAATTGTGAGCATTACGTTCATGCATAACCACGAGAGTCGCGCTCGCAAATGTCGAATTGGCTCATCCGCCCTATACTATATAACAGTATTAGGAAAATAGACTCTAATTTATAGTAATTAATACTTCAATTTACAATACAACGTTAATAAGAATTTTTATTTTATTTTGTAACATAAACAAATTATTATTATTAACGAAAAGGAGCAATACTTACGTTTTTCTGTCAAGAATGTAAGTATTGCTCCTTTATTTTTCAAAAACTCATATACACTAATCTAATATAGCGGTATTATCCATTTGTAGATAAGACTTCTACAGCAGCTAAGTCTAGAGGAAAATTGTGAGCATTACGTTCATGCATAACCACGA0J[cv%4?EQdv.=FQYbnw,5?IR\iv)3@JU`kv(3?KXdoz $0:EQ]iu&1=HS`kv ",7BMZfq|    ) 4 > J T _ j v   " - 8 D O X b m y    * 6 B L W b m x  , 8 D O Y e p z  ! 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$("=5MJ61+0 &"'!6)% !#$(64H:'2$)/0-3/#'!/.+0$!L-471?E:MIjNC w% ]&AGAGGCGCTCGTGGGCAAAATCCCGTCGAACCTGCGATATATAATATATCACTATAATTACCGTATTCACAACTAAATAGTCCCCCTTATTCCACCAAATACCGGACCACGCTTATCTTTACCCCCCCCACAAGGTAGGACATGCACCTACTCGTACGACGTACTGGTTTGCTTTCGTCCCTCACCCATCAGACCATGCCCCCCACAGTGCTTCGGTAGACGACGGGCTCACCCCCTCACCGGGGGGGGGGAGCCGCACCAATCGGGAATAGAGGAGACGGCGGCAGGTGCCCAGTCGTGCCGGGGAATGTGATTACTTCCCTTCAATGCGAAGAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:09:39 -04:00AEPt3AEPt3APFN APXV2APrN ?APrV1APrX}}HARTNASPFASPtASPtB1PtB1PtBCTSBufT"DCTIDCTNMCTOwCTTL  CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DFDATA"DDATA"DDATA"DDATA #GDATA #G-7DATA #GtDATA #GDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ‚EVNT ŽEVNTšEVNT­FWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~sLIMS!!LNTD2LsrPaMCHNMODF 6MODL3730NAVGNLNE`NOIS=OfSctP1AMQP1RLQP1WDQ<P2AMQP2BAQQ"P2RLQPBASQQPBASQQpPCONQQ PCONQQ VPDMFmPDMFTPLOCQ PLOCQPSZE`PTYP PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܩRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""ܹS/N%5SCAN3SMED SMLtSMPLUSPACMSPACMSPACMSVER4.0SVER SVER,,VSatdt^Scal@Scan3TUBEC4Tmpr<UserphARphCHkphDYbigphQLcphTRphTR(GThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ?APrV1APrX}}HARTNASPFASPtASPtB1PtB1PtBCTSBufT"DCTIDCTNMCTOwCTTL  CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DFDATA"DDATA"DDATA"DDATA #GDATA #G-7DATA #GtDATA #GDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ‚EVNT ŽEVNTšEVNT­FTab-WFVoc-FFWO_GATCFeat//-GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~sLIMS!!LNTD2LsrPaMCHNMODF 6MODL3730NAVGNLNE`NOIS=OfSctP1AMQP1RLQP1WDQ<P2AMQP2BAQQ"P2RLQPBASQQPBASQQpPCONQQ PCONQQ VPDMFmPDMFTPLOCQ PLOCQPSZE`PTYP PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܩRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""ܹS/N%5SCAN3SMED SMLtSMPLUSPACMSPACMSPACMSVER4.0SVER SVER,,VSatdt^Scal@Scan3TUBEC4Tmpr<UserphARphCHkphDYbigphQLcphTRphTRa,pEnO"#G)jp~0,W(YzX|s$4/YCC"*0RgKtj({W_zEUWo VgEM OkoWvWv9k]ty6Ka;` "Gq.aUPj(K3qoIH}7a2&d:Dp$^HAp2 ^m%bVm? VCCHY<6(mu y-WLa)&:\.~x|MI9?";!8L[2Rr`gPV]O_,~])RpikE:b1AmX-RMLr92U*a!(G2MZLNN1O3WAd@EaZL)E^rkrOW9;(dC18{ZWFrbT;fQGKekx&#v3"qo,D`&Tj1t9z"DEoh90S*o_cM&+#u?GG5)ljD_P?Yf]4'Uw\Pcc{\A'ZvXTP>I_n@(Zf.kRr+4#3y4E^h9r4uoak"T'o7LA;mq;;9VgO(_y"`k:!.tkSY4E2 (#W+6,j'@pnZy:pGqK_V&S$~=}F)h>;-T)RJTZmU)P? sIe]s1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOW1 *>k4d;$H_&kGyK0&ML(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0FkV@=[OX&ts57\3zH'WzzqbB;uFbH@O!p8NpdNmbcnPl'Z9dEe/D?*{|D$haygK >xP `v?{}I8w$YWmOyl}$rv/8NpFQlUAT/n@Z-I+TwM"f^@/(-[mMOMh7&05OPh~wA[h@tFt(#jyO~6X~3"F*+#dgU[_5:W^$OufcGHO5QfOl>o ZbCd]'GE#!#hmmfc4.=+I}S$o:/bEmKB6KP8)s5AVihx_7&OMb=KuRZ+kM7yW@"SD=y!.qK|-o*u0#|UUQ1V? M;8'i2{ysPl`A(.:U q<6(mu y-WPv8U)HJxmAEPt3AEPt3APFN APXV2APrN ?APrV1APrX}}HARTNASPFASPtASPtAUDT~~=*B1PtB1PtBCTSBufT"DCTIDCTNMCTOwCTTL  CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DFDATA"DDATA"DDATA"DDATA #GDATA #G-7DATA #GtDATA #GDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ‚EVNT ŽEVNTšEVNT­FTab-WFVoc-FFWO_GATCFeat//-GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~sLIMS!!LNTD2LsrPaMCHNMODF 6MODL3730NAVGNLNE`NOIS=OfSctP1AMQP1RLQP1WDQ<P2AMQP2BAQQ"P2RLQPBASQQPBASQQpPCONQQ PCONQQ VPDMFmPDMFTPLOCQ PLOCQPSZE`PTYP PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܩRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""ܹS/N%5SCAN3SMED SMLtSMPLUSPACMSPACMSPACMSVER4.0SVER SVER,,VSatdt^Scal@Scan3TUBEC4Tmpr<UserphARphCHkphDYbigphQLcphTRphTR