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"'$   $'.7T>.   3;2H=>'%+%!C;eXclW_R;P5C6?9QML^fRzs &Gb[Zfll~kr{~qbmX82zvebm`ZE?%(#$ -)"-!         -19&%  ,!&   56&410   "*':M: !    405(!'"'5A@4:2I0"$"<$< #*     -"  "B501!                            ! *3'4""-,&  ..3E`|mLI@<?.*1*    !4!%$ ./79dlRPP1J9KaTU@4C.8!.&.E0);;)&& !         ,$"' +$&#%"# " ;*&%-"#5#$ +)%                                                                                                                                                   $                              '                #                 d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 010f50bc8f46496a88167b18c14f660dSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_C_3_Lozanella_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7z .MtP"O|7D|kp  7 `  q*s gP  _  n .neC :x@9qRagA=c?!%<RagcYK</"nU=$ ~|{|||zxvvz 4jgT]jjF|liPCRN ?g "#$$% $$$" lX L - l ol$,B{? ytpoujA916[g]=@UzzT~S l%r1Fv +,3d$}B|wsokihghjoyb61bLd)1ELA"]`rbXSQPONLJHGFGIMSZclv~|qdWJ?5.)&%')+++))*.3:AHMSX\`bb``drL iT(kRFDEHIIGC>93-($!  &-36762.*&$!       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"$(.7G`I>~eD* l*cL<2*%!    )17:::98863.' $Ue:%GF E1;goRe }O/ '`1WV!o:8t. hyfn <G%d\O+ 3Z\JB lV#J >bkO AE=`[d,(g e{Vm2T )fXdOBB1"QA\v6 8t}paAB4TJ#7 4 ?Q+P   :+O3W,sQ@1mOgN!W2ru6 +veCpb4e*  )rcKzj&p=@tKNT`HDKU]bfkq{fXUZi^SJJ^K :1$b' D9L%Z~7     1|G\2x+1%?\EdZ& L]wWs fH@`C k} 26U5aGw:V{0^*J)Wr, : @jbe   )qO[F8shKHaavo']lN f8 PYT^OOW_b\J5'3ic M.%U;:w8 %k=DhEH}Vo1P  .w U{\c$30^  fT&?*`R``Su)'o]s<tlP ;Bd" JjLKV!F,LnRyCE}7BNLJ-9>;/!*9CE?3$  (/0+ &)%  !(*'    ! !#!  "(-.+&  !!   "!                    "'(&!                               #*/0-%+=MX[TG7' 1l OwyUI( ,Hd{ybH/       "$&(*.3:BJQW\agowxqje_YRLGCA@???@@ABCCBAAABDGIJJHEB@?>>>=<9631001122112469<>@ABCCDFHJKLKJHFEDEGHJKKKJHGFEEDDDDCA?<:99;<>>><;99:<>?@@>;8532220-)$  !'+-..037;>><9531110-)# B%>nE  =R/Yc`y'BU\S<b=@oR *AML:_x5-BKG6i;'c,e` fuaSG?951.*'$"""#$%',7JfJ Tt~oJ:fK9/-4Fe!}@1auL3($%'*,./0000//00111/.+(%!   *>aI0/TBLqqU4;s64[E SzxK1}@R x`y j<"  ,kEY P !$#T%-&']%%b<?V# Q.N@$L c[!6  6 VNk=1Q=5f)DS>SWMd"^`V,KQUv8\> A$JO!VU!VW2hQ^   <MfBn1 Q.NJ &R #puo'y..{E=ET3E?8Q  (1<K\m} /<@;/$(Iiu Fw<nyPp`HX   -{1"I  D)9WVc Ay,7K/R#S~7&j@U    T=A~/ 776PL^  &q]$;<a! &mR>X{4     (vxm89n@5AXpwlbYQKFB?:4-&    #b!}SI    6[P@  Ye~3;   8~.q}DV# #,0/( %-0-%")*& #*,(  #'&  #''"                 #%&# &-4763-($"#&*.021/,(#                                              !"#$&(*,..-,+++,--...--,,+++,,--,*'$"!!"##"      !"#%')-0368<?DHMRX^fmtz'<Oaq (3>FLORUZaiorqkbZSONMKIHJR`spU:GaMasSj2}zxvtrnjfb``beggea\XUTUWXXWTQNLKKKKJHFDB@@BEKS\fq~.DWhw}lYH8(+^i|?     tweH:2& C /Ks (ZRLKdgQ$=~ r$k=pV>)  0Uw? i"3-jL^x|F{xtplheeip{K/NYP2E$xmck*9YLjkV6 0X}Z 1WrO+yqC $Mz  x${zzxtnhb]YVRMIDAABDGGF@8/%#9e %VQ/k5 &-5=EKOPNH?4(  ,lKk2I%L,Z"   !$$!   iw9vy2  "$&&%"    4zHJl,       #,6AN\l|$Gm7Nao~vq;ltN2uaO@4,&"    1B9f1"*/110/-)% !""! $(++)%!    +~Sh:Yx7"#!     "U,JGP68Iav|wJ$l~Uy|#wh[PE;1'         !8OaieWC. "&$ ',,(!           #&*/4:?DGJLMOQTX[^`_^[XVUUUUTROJE@;852/*&!                      $+.-*$   &0672*          $@AF ASA~KB.bcpKB_3730_POP7_BDTv3.mobtermAAAGTGCGCTTGCAACATTGTCGAATTGGCTACATCCGCCCCTATACTATAACTAAATAATATATATTATATCTATTTCAATATATTTCAATTTACATATGTTGAAATAAAGAAAAAAACTTATGTAACTCAAAAGAAATAAATAATAAAAATAAAGGAGCAATAATAAACTTCTTGATAGAACAAGAAGTTTATTATTGCTCCTTTCCTAAAAAAAAATCGTATACACTAATACCCAAGTTTTATTCATGTGTAGAGGGGGATTCGGAGGGGGTAGGGCTAGGGGGGAAATAGGGTGTGTTTTGTGTTTAAAAGTGCGCTTGCAACATTGTCGAATTGGCTACATCCGCCCCTATACTATAACTAAATAATATATATTATATCTATTTCAATATATTTCAATTTACATATGTTGAAATAAAGAAAAAAACTTATGTAACTCAAAAGAAATAAATAATAAAAATAAAGGAGCAATAATAAACTTCTTGATAGAACAAGAAGTTTATTATTGCTCCTTTCCTAAAAAAAAATCGTATACACTAATACCCAAGTTTTATTCATGTGTAGAGGGGGATTCGGAGGGGGTAGGGCTAGGGGGGAAATAGGGTGTGTTTTGTGTTTA4Ibw&+6AGSfx (0>GRZcox !,7DOZdp} &2;EQ^jt(5@KU`ju(3?JU`it'3?JT_jt  *6BNXblx  " - 9 E P \ h r |   # . 8 A M Y c q {  ! * 5 > L Z e o {   * 4 B R Z d m w    ( 4 ? K X c n z 4Ibw&+6AGSfx (0>GRZcox !,7DOZdp} &2;EQ^jt(5@KU`ju(3?JU`it'3?JT_jt  *6BNXblx  " - 9 E P \ h r |   # . 8 A M Y c q {  ! * 5 > L Z e o {   * 4 B R Z d m w    ( 4 ? K X c n z   2 %2(/2!:### :4>.(>8>8>8>//>>>>>/>>>>%/>>>>  $ -+'>>>>>>>;>>>>>>>>>>>>>>;>>>>>> )"3;83;>>>>>>>>>>>>>>>>>66>)2;;>3;0 8;8821811."("""3;3"$)"'+3  "   "$"  "      "        2 %2(/2!:### :4>.(>8>8>8>//>>>>>/>>>>%/>>>>  $ -+'>>>>>>>;>>>>>>>>>>>>>>;>>>>>> )"3;83;>>>>>>>>>>>>>>>>>66>)2;;>3;0 8;8821811."("""3;3"$)"'+3  "   "$"  "      "      6-B55G>,-b>3STE!%#V(bI9B6CG'5G]1G< J5+J#84E3)=U8X81$<E3(IG0T@BJ(4$ XU^)]^$)>7"L)Y2bDT]WR:5, "@^9_IN@BbZ.H!*J%!"4_/V ]6J8P^-!<C!_&+:U b[.13H"KMYN(P<(WGH1\*9-0!8c+!LY_@c2\ccCK==M3ZS c2-6=c2]8c9GV <cG+9 S&J27B"5j\J= ) ]sy[2y(}y<wScyIK qN-Y)^{%_Co<<m./~Y Ex:-V'Sy$&6 Hz'E#P%]m?X"'~Nhd  Dhy-: ;  dT "l]3Y`kQ \M jV7G1s0  k $xa;DlS9bu8 {1pYz{RORE`h_[d,>d  ` `9a^[3l usgg[W^]xDAUa]1WQ4>,A'%* $&4  "& ?      9% 3#5U;-b\ckyU 1#=,# spapTp H[{4pEpazK`txJ}]3pgppp)\f#N2p}]7 -K8vAH`bpe/ dM98,YbS0%4nyQsHWq,&X@c:` G ~T'z*q[[R[Z@cv37*ASeQOE.`j\?b9"; .X// -J%:UJf6UF,2*9.#*C  CK *#JWfMC/\   W!Y 2@X_~lNiQirmb 3 iewh<wf|ZKv([CBSgo-7Mkb7'U l (<@k,|M u GGGAGCGCGCGTGGGACCCAAATCCCGTCGACACATGCGAATATACAATATCAATTGATTATATACAGGATAACTGGGACCATAGCCCACTAAAGACGTCCGGCGGTATGGAGGGGTTCACCGGTGGCTCTCTTGAGGGATTTCCGATTGGGATGGACGAGGCATTCCCGGCGGCGAGACACCACCCGCAGGACACCGATAGGACCCATCTTGGGGGTGGATGCCTTTTGGGACAAATCAGGACTCCTTGTGTGGAGTACAACAGTACGCCAACGCTATACCAAATACGGCATTTAGACAGAACTGTGGCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:09:38 -04:00AEPt3AEPt3APFN APXV2APrN oAPrV1APrX}}xARTNASPFASPtASPtB1PtB1PtBCTSBufT"DACTIDCTNMCTOw1CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA #GDATA #G-DATA #GuQDATA #GDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ²EVNT ¾EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~1LIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOISOfSc#P1AM7nBP1RL7nP1WD7nP2AM7nP2BA77P2RL7nPBAS77SPBAS77PCON77 PCON77 fPDMFPDMFPLOC7n PLOC7nPSZE`PTYP9PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,†Satd#^Scal@Scan3TUBEC3Tmpr<UserphARphCHphDYbigphQLcphTRphTR,́This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN oAPrV1APrX}}xARTNASPFASPtASPtB1PtB1PtBCTSBufT"DACTIDCTNMCTOw1CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DvDATA"DDATA"DDATA"DBDATA #GDATA #G-DATA #GuQDATA #GDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ²EVNT ¾EVNTEVNTFTab,FVoc,FWO_GATCFeat..+GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~1LIMS!!ELNTD2LsrPaMCHNMODF fMODL3730NAVGNLNE`NOISOfSc#P1AM7nBP1RL7nP1WD7nP2AM7nP2BA77P2RL7nPBAS77SPBAS77PCON77 PCON77 fPDMFPDMFPLOC7n PLOC7nPSZE`PTYP9PXLBRGNm RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,†Satd#^Scal@Scan3TUBEC3Tmpr<UserphARphCHphDYbigphQLcphTRphTRR)U`l5j6!X`7.qT*uq&6i}8Amabj1A6rd)ziA=sUOzj{>ZHm? V<(?:13Uv|Q{9V \+Yoe2{sGi=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN-&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`G]p-@Qhiq|fq'#kD:_f7o,f9r;qBwJUo6;^UWZi7WeOzJu%~4%rIZHp#)+KT>:L-Ev!$V@AKl&O`x Pz5's1Hhl@tt/rpi)3c+FJt$++V**~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKK-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wT\3>>B]'p8Npd-(bcn23isFb$t\:+be=I@=0zEp(CB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVoJ@GtWStm,n`v5H4lWqTXYoFsO18 YhWW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG