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"                      d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 325c0d0065634a4d9c64c27a6968e744Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_C_2_Aechmea_sp_trnhKB_3730_POP7_BDTv3.mobSeqPop7z(6GYn5YGx&c7eEgi7W#{;}E~T1 %5FXk #*..( qO)x_I5$  xqjd_[XVTRQPPPQQRRRRSSTTSRQOMLLMORVY\^adkt6`$WV'~_E0"'7MkskaUY c h ^ :  M d ` QC?MnTd}cO>0$ $6HZn"ceU'p   z    v *,A9n@4A^7IOG3[%b>" 2n*Z}rS/ )F[ecV@#\0qf`^\\]^`dhmrv{|rfYL@60--/147;?CFHHFDCCDGKNQTWZ]^]ZUOIEDFMYi}x^G5)!     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0_9]#,2D:&EL+ 22-_9I _N#pp:L dW!y dG=c)OR ]c}aMt1nI}Q&J~$5cY>_Tt;No&yB>"oh'qW:hO-81Pe7t78uJD  L$Zw?45w2rEUO (dT.Y`=OVEv>^9gK}.tF}E!$GAZ?&IEsY3`m3FMkgV9gQ`|XfJ08iZ\UQ2E+S^x?  k`ZVS!J M1? 8X{(Z;qsh&'"KNtDi=;KzQmZ:\"QhT?7#sN.MHy`a^Q"5j+}l0H8% |L^]/sS{'pV 6P R,J C5o@*(rie_[abNV;OW0'`):~ q1","  '                  2   (    =!   #+$#) g$D#O` >1,.'B^\.-F%,V)(11tA8@D ?(((05$'F''V'o  I Z''1113%%'''%%''% %  ''lK%g''    %3%'M' '%% 6''k'' !8r>=*pfp5R;X \we!R]@htd:yXBpH*Hk~ZQbxd;Dppy55AN# A(I,x_W`W.\gA8b$-3cl_{+_7 jZ*.>ripPO;*pC8  ,^p641AYD6Mbhp~}0pq#VbZlhOz$jw+QN&)Z\xfH5C4*N%Hp@k A tu813F|ZNp0kHFp/TtMRU56 \%`#N#Nb 1SXr; 5ny&:)d4! k)7+-15]1$/",dx@XC$$)-"/)4+2$)SrPxS,``)$*7&! #"$  +2 "&( %.#znBA8+5%#)" &0'!.(!%7%W!?/" &#' $"*$%)* -+ '"%#."61$!C%+$!!:3&7*."%E1!10L$3$''#,'!*=*0%1@4GO# 1+$D-%4"9%(7@3.1)32'.^R%UsD.1*!&S;K&+B#W0eU#"~U3Ep3lMUH`-RYh+iCaR% mDZ=aI`P+a-{uZku{<8~mXzgP>y;$(EPX(7P[vryz$&in8AGAGGCGCGCGTGAGACCCAAAATCCTCCACAAACAAACTATTAATATCATATATAGATTGAATACAATATCCAAACTACAAACCACCCCCAAAGAGGGACGCACATCTTCCCTCGTATAACGATATCCCCCATAGAGTGCGTTTCTCAATTTTCTGTTCTTTTTTCAGAATTCGCCGCCCCCACTTGCTGAATTCGTTTTTATTCCCCTATATAGTAATACATCGCATGTTTTAGTGTTCCACTCCATCTGCCCGTTGTTGTTCTTCCCCTCGTTACCTTGTCTAAAGGAGGCAACCATACAATCACCAAGGGCCGGCCCACCATCGCGCATTAGTAACGGGAAGGATACGTTTCTAACTACATTACAGATTGGGTTGGAAGTTATTTAGCACGAGTAATTGAGTGTTCTCTGTAAGTTGCGAGATAAGTGAGGGAATATTATTTATCTTCTTCCGGCAGGAGAGGAACCCCGCCCTTGGGGGTGGGGAGGGGCAAACCCCCCCCCGGCTTTTCCCTGGAAATATGTTCTCCATGAATTGGGGGGCTTACACGCCCCAATCTGGAGGGCTGGGAACCGGCGTAATCCCCTGACCCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:09:25 -04:00AEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPt&ASPt&B1Pt%B1Pt%BCTSQ?BufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA 'OjDATA 'Oj5DATA 'OjkDATA 'OjDCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~OLIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOIS#GOfScxP1AMT:P1RLT6P1WDT?jP2AMTHP2BATTMbP2RLTDPBASTT%PBASTT#zPCONTT3PCONTT1rPDMFwPDMF#^PLOCT,PLOCT("PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%#?SCAN3SMED SMLtSMPL]SPACAkM(SPAC#WSPACAkM(SVER4.0SVER Q4SVER,,^Satdx^Scal@Scan3TUBEC2Tmpr<UserphARphCH#uphDYbigphQLcphTRphTR/This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN GAPrV1APrX}}PARTNASPFASPt&ASPt&B1Pt%B1Pt%BCTSQ?BufT"DCTIDCTNMCTOw CTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DNDATA"DDATA"DDATA"DDATA 'OjDATA 'Oj5DATA 'OjkDATA 'OjDCHTDSamDySN Dye#DyeNDyeNDyeN DyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT ŠEVNT –EVNT¢EVNTµFTab_FVoc_FWO_GATCFeat//_ZGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~OLIMS!!LNTD2LsrPaMCHNMODF >MODL3730NAVGNLNE`NOIS#GOfScxP1AMT:P1RLT6P1WDT?jP2AMTHP2BATTMbP2RLTDPBASTT%PBASTT#zPCONTT3PCONTT1rPDMFwPDMF#^PLOCT,PLOCT("PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%#?SCAN3SMED SMLtSMPL]SPACAkM(SPAC#WSPACAkM(SVER4.0SVER Q4SVER,,^Satdx^Scal@Scan3TUBEC2Tmpr<UserphARphCH#uphDYbigphQLcphTRphTR~WhC0-#JpPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!-%`qpi8muNy,U94BEU{#(cO9+!!f|bEdGn~'_26"nlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfmkbB,WOsX~M]pyBCQn{4J`uJ\LKZI\T(k"T'o)6yHAj}=D8m%(4Ws _ZZ^sSsYiSOpP S!oSRb;6AI8rBA(&jEaC9}RiTK(>V&bJ~FTWYhY8|kb+N4+~[{Hu#e./f<@j2QuOzcI0 Vps5\iDhkkJ/n=dNL?>\&juZ *[K3'MI#6mOgkddQl& T.+6P-2ZE<}qjM>Pz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*PvMODL3730NAVGNLNE`NOIS#GOfScxP1AMT:P1RLT6P1WDT?jP2AMTHP2BATTMbP2RLTDPBASTT%PBASTT#zPCONTT3PCONTT1rPDMFwPDMF#^PLOCT,PLOCT("PSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܱRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N%#?SCAN3SMED SMLtSMPL]SPACAkM(SPAC#WSPACAkM(SVER4.0SVER Q4SVER,,^Satdx^Scal@Scan3TUBEC2Tmpr<UserphARphCH#uphDYbigphQLcphTRphTR