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' 5 ' ! ( 4 # , # * + ! " ' . & * - $ " - ' ' + . ( # + - % ' 2 ' ' $ ( ( ( ( ' * - % . " " ! % & * ( ! ' ! 8 ) - $ 8 ' & + $ " , % 0 % , " & 0 - " 4 , ( ! $ ! . + * 2 & * / ! 5 # & 4 ( ' / + ' - % - * , ( 6 " # ' + " & * + 2 . % " ) , 1 # % $ ! + . * ) , ) 4 ) ' 2 $ / 2 + % ' / ' 8 $ & 5 " , * , * * . " - 3 - & 0 ) 0 - ( 0 5 , 0 * " / 3 . $ " : ( / * ' # / / ) % + ) & % + & ' 2 - # $ ! 5 # * ) . - * " $ < " ' 3 4 * 4 ; 9 , 0 $ I : E : 5 8 ) % + / 2 E 3 1 2 2 , 9 0 8 ' ' ) , , . + 0 8 d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 3}{xwtrpn P P P H H H e f f } } } (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}| < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1365554d41a444219d9f0f0c1809a522Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2017-04-14_06-39_1995 3130Seq 10143 10143_Lehman_K1361_trnh jmadera 96-Well K1361_B_8_Lycaste_deppei_trnhKB_3730_POP7_BDTv3.mobSeqPop7z r t w | !1BTg|1Kf5Ngv\A%xU3zgYNE>8435:BKU`kvmZG4" ~ y t o j d ] U N H C ? = : 7 5 2 / - , + * ) ' & $ " ! " % * 1 : D P ^ o +Z Hng>N w q o o s z 7uzl)UP (wxN*.h oWC2# ~ x u t v y | "Hu4[ ;S`bV> XejO;)
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X RR G6 ZJ dEDUU;Rws v q$>z C+=;9c_9pXI s; XBAGAGGCGCTCGTGAGACCCAACACTCCGTGCACACATGCTAATATACAATAAATGAACACACATACCTACAACTCCAATCACACCTCCAAAAAAGAGGGGGACCTAGAAAATGGACGTGGAATGCCCCTTTACAGAATTTGGATGGGGGACGCACAGCACGGGAAGGAACCCACCTCTTAGATCCATGTAGTTTTGCTCAGCACAGGGGGTGGTTTGAATTATTCGTCGGAGGGGGCGATACAGAGGGGTACGGAGGCAGGCCCCAACCAACCCCCTATTGTGACCTAAGGGGGGGGCATCCACCACTGGAGTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82017-04-14 08:10:11 -04:00AEPt 3 AEPt 3 APFN R APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt
B1Pt
BCTS B BufT " D CTID ܫ CTNM ܱ CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D R DATA " D DATA " D DATA ( Q [ DATA
( Q 6o DATA ( Q DATA ( Q ؗ DCHT DSam DySN µ Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT J EVNT V EVNT b EVNT u FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ A4 LIMS ! ! LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS ) OfSc X P1AM : t 6* P1RL : t 3 P1WD : t 8 P2AM : t = P2BA : : ? P2RL : t ; PBAS : : + PBAS : : ) PCON : : 2| PCON : : 1B PDMF ; PDMF ) PLOC : t . PLOC : t ,Z PSZE ` PTYP PXLB RGNm ܣ RMXV 1 RMdN ԍ RMdV 1 RMdX Ԗ RPrN q RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT RUNT * RUNT Rate RunN " " ܁ S/N% ) SCAN 3 SMED
SMLt SMPL SPAC Aj9 SPAC ) SPAC Aj9 SVER 4.0 SVER B SVER , , Satd X ^ Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR 0 This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN R APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt ASPt B1Pt
B1Pt
BCTS B BufT " D CTID ܫ CTNM ܱ CTOw CTTL CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D DATA " D R DATA " D DATA " D DATA ( Q [ DATA
( Q 6o DATA ( Q DATA ( Q ؗ DCHT DSam DySN µ Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT J EVNT V EVNT b EVNT u FTab Q FVoc Q FWO_ GATC Feat . . P GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE > LAST ~ ~ A4 LIMS ! ! LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS ) OfSc X P1AM : t 6* P1RL : t 3 P1WD : t 8 P2AM : t = P2BA : : ? P2RL : t ; PBAS : : + PBAS : : ) PCON : : 2| PCON : : 1B PDMF ; PDMF ) PLOC : t . PLOC : t ,Z PSZE ` PTYP PXLB RGNm ܣ RMXV 1 RMdN ԍ RMdV 1 RMdX Ԗ RPrN q RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT RUNT * RUNT Rate RunN " " ܁ S/N% ) SCAN 3 SMED
SMLt SMPL SPAC Aj9 SPAC ) SPAC Aj9 SVER 4.0 SVER B SVER , , Satd X ^ Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR .iTr8p|_1{63_`EHsMT@OmAmDe8HR/D2GcA13woF$1PI?p":*Xq)apMf4S><3$G,lwd-l#frL?*Y\FX`@kjJ}B~xGH$i*8j|]]u.ibuILW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A\&C9":?ZEq1hu$#k:X=<;3On&+VN;172e,qz7|!Y<,h`Fzmj7Ul*$ml1Rc4K(G_[6!9'qV8JX|K2-!;9xxsWD@|DmOY,f *"49J_'JKxwQ4fC\{lKd#8 UC;2i$@jEl0&7dEc"O7_/Y'"jx,;h^b@,kG<7s+2U*7k'}&8)TK7kx`rxP!t p7Zf_@72tX>VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8K%}jxz)>`VWYhY8|kRYN4+HmL`Kr0k@\-/2ki;j#MQsB0aKjZD(Z=c':Z^mDt4)TuTF}wKy3of)`|8&kS2BjrP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]|UKo9UC|T[[66`aD/ !HRw)QN2P,pc+Q9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]w LAST ~ ~ A4 LIMS ! ! LNTD 2 LsrP a MCHN MODF MODL 3730 NAVG NLNE ` NOIS ) OfSc X P1AM : t 6* P1RL : t 3 P1WD : t 8 P2AM : t = P2BA : : ? P2RL : t ; PBAS : : + PBAS : : ) PCON : : 2| PCON : : 1B PDMF ; PDMF ) PLOC : t . PLOC : t ,Z PSZE ` PTYP PXLB RGNm ܣ RMXV 1 RMdN ԍ RMdV 1 RMdX Ԗ RPrN q RPrV 1 RUND
RUND
RUND
RUND
RUNT ' RUNT RUNT * RUNT Rate RunN " " ܁ S/N% ) SCAN 3 SMED
SMLt SMPL SPAC Aj9 SPAC ) SPAC Aj9 SVER 4.0 SVER B SVER , , Satd X ^ Scal @ Scan 3 TUBE B8 Tmpr < User phAR phCH ) phDY big phQL c phTR phTR