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"           "                &       "           d,,,III^^^{{{&&&999DDDXXXaaavvv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}{xwtrpnPPPHHHeff}}}   (''/0/887@@@HGGOOOVVV]]]ddckkjqpqww|}|<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ae4038b491374e829187d39c8ece24c8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-14_06-39_19953130Seq1014310143_Lehman_K1361_trnhjmadera96-WellK1361_A_1_Bejaria_aestuans_trnhKB_3730_POP7_BDTv3.mobSeqPop7z +:L_t%FlK}1x N4j3\sELo"4`'}bNCAFP^m}?_zkJ'Y.gN7#  $.9CKPTTSPMHD>7-~{xurpnmlkjjkmoppnljiikmnnnmkkklnqty!IvQ:mj@uCxlhmx;8  C a^,. u=? 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"$"                         s?@rAvAHAB[KB.bcpKB_3730_POP7_BDTv3.mobtermGAAGTCGCGCGTGCGACATTGGCTCGATGGTACACACCCCCTTTCGATTTCATTTCAATATTCATCATTATTTCTTTCTTATTTCGGAGATAGAAATTTTTAGTCGTAAGCAATTTTTCTTTTTTTATTTTCAATGGAAAATAAAAATGTCACAAAATTAAAATAAATTAAAATAAAGGTGTATAAATTCAAAATGAATACAAAACAAAAAGAAAAAACGCGACTCAATCATAAACCAAAGCATTAAAATTTTTTCTTAAAGCGAAAAAATGATCTCTAAATAAGAGACTACTCTAGTCGTAATAAAAAAAGTAAGGGAGCAATCACCTCTTGTTTTATCAACAGGGCATTGCTCCCTTACTTTCTAAAACTCGTAGAGACTAAGAACAAAGTCTTATCCAGTTGTAGATGGAGCTACAATAGCAGCTAGGTCTAGATGTTTGTTATGAGCATTACGTTCATGAAGCACCACCAGAGAGAGAAGTCGCGCGTGCGACATTGGCTCGATGGTACACACCCCCTTTCGATTTCATTTCAATATTCATCATTATTTCTTTCTTATTTCGGAGATAGAAATTTTTAGTCGTAAGCAATTTTTCTTTTTTTATTTTCAATGGAAAATAAAAATGTCACAAAATTAAAATAAATTAAAATAAAGGTGTATAAATTCAAAATGAATACAAAACAAAAAGAAAAAACGCGACTCAATCATAAACCAAAGCATTAAAATTTTTTCTTAAAGCGAAAAAATGATCTCTAAATAAGAGACTACTCTAGTCGTAATAAAAAAAGTAAGGGAGCAATCACCTCTTGTTTTATCAACAGGGCATTGCTCCCTTACTTTCTAAAACTCGTAGAGACTAAGAACAAAGTCTTATCCAGTTGTAGATGGAGCTACAATAGCAGCTAGGTCTAGATGTTTGTTATGAGCATTACGTTCATGAAGCACCACCAGAGAGA7R`h)6ELW_iv />FWer{)4?MY`p{)3>LXdp~ $.;ITany $1=IU_jw*6BNYfs%1=IVamy  " . ; G T ^ j v    ) 5 B M Y f r }   $ 1 < H S _ k w    ( 4 A M Y e q }  ! - 8 E Q ] j u )6AMZfs #/<IU`lx #/:EQ\ht,9DP\hu'4?KVco| #.:GS_lw+7EP\iv*7DO[gt&5@KXdo|7R`h)6ELW_iv />FWer{)4?MY`p{)3>LXdp~ $.;ITany $1=IU_jw*6BNYfs%1=IVamy  " . ; G T ^ j v    ) 5 B M Y f r }   $ 1 < H S _ k w    ( 4 A M Y e q }  ! - 8 E Q ] j u )6AMZfs #/<IU`lx #/:EQ\ht,9DP\hu'4?KVco| #.:GS_lw+7EP\iv*7DO[gt&5@KXdo|        '! '&&&&.+>;,,'&+','")""'888&+"1"&"+3;3811;80$++--2+'.2;+'++)3+&".(+;..(81+2.+1133;328288222;;;;;8>;>>>>>>>>>;'1+.&;..+38+32.13;;;;;>>>>>>;;;>;>>>>>>>>>>;;88;>>>>>>>>;888;;;288;;>>;>;;;>>;>>>;>>;;>>;>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>;;>8.>;;>888;>>>>>>>>>>>8;;>;8;>>>>>>>>>>>>>>>8;;8&8+>863>>8&>>>>888888>>883888>>882>8>>+38+2>3>28++&288+8**3"   &28+.8//8&8 >*          '! '&&&&.+>;,,'&+','")""'888&+"1"&"+3;3811;80$++--2+'.2;+'++)3+&".(+;..(81+2.+1133;328288222;;;;;8>;>>>>>>>>>;'1+.&;..+38+32.13;;;;;>>>>>>;;;>;>>>>>>>>>>;;88;>>>>>>>>;888;;;288;;>>;>;;;>>;>>>;>>;;>>;>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>;;>8.>;;>888;>>>>>>>>>>>8;;>;8;>>>>>>>>>>>>>>>8;;8&8+>863>>8&>>>>888888>>883888>>882>8>>+38+2>3>28++&288+8**3"   &28+.8//8&8 >*  b7,'FI4/X*;YL%9`42 H:*66H,OQ^4H`]IX+*5 )'cJY0=J$I 'HRZE( ?2U7L"/M!SXQ@'ZOI>- 'NUQ4"GP8_-KUEZ?2;)NFCD_S C3@<;)C(9@Q'@"aa[&'@*=O/-TO^?(._$% Ed^#ON#Q OW( F-PMP3 URVLF$S:4 _ +% H,d 0H@1"/&*9Y&K(3(9B++-GM%0;$W,^!$A&cQ!RbIM76!,.LJ4a6SCL!-"<D '2GKVC87 9&IU[b.*59#Z7M&(. +*C' 4 _ 72cGSB\M9T0J >UH,-G&3U7'=@ .Y%7B_I_5L%*<N"P"._=XU QTP!@d;+Wb"00%>c4I.31%S72Z 7R:(H d9ZK2Z ## =##O 2H4w% >^ yA e  =l;v `7,LX~5Yk^~W */}rM0F0 ?4vKG(;|tP|u/+]bA|np t]:m0*zR!5t4]UBPiV)aoMtl$+BkFXLV @ E\x#4.c=3sYJx9MF9Vqr -}Mn+^&kv:of kqHzG?9V/z'nm "LIb ;!rNg9|81mUfyT+{WlEfcZ.{ C` !S_q2e}8=>,{71 6Laof} u0 T2D2e[rX;.G6I\Zrb 4B Y89Qq#,L2)!' .436 ,xxxx%           /      ? +B"5 ,25M#  B&*5.%.!%$- OJ!)a @"R /" *.t&/?!"?@KV8"""""""""""\",e3dapH`pd)3$=q7Nb*"n1pzC'7)7 HnX,h%#u%~!+%=s{ARd^-oB8duCd *%1?&jOk6IBY'dF\ hezdd{PqX(,!:I-)(d &J&CB>d'{ )mYD7;#SI#>$H_P&yppT&7/^ a84j\Gbpp4Q-pDD8pZb ^l?p 3Jd* 3:%Z!4S w9ty}R 9*J5JE-sbI#DK;" #{8j0K?O.W!U:{,)MwQsiTx:zZkR='&F87d{ZC4EK|';UlUJAG/IDAEZ.R:i2{!Noy(3><)MpS8B.C'}H$*'46"0-5Z&4%oNQ=]S]fQ@HI36q~0X%*6&S181;;eG# ^`5hI=.D=5B"I"J1-#61/'>W67!Y.U6**Mu@qF@&)E~,7+21;77!0M>sc`vo_e4FM_Q-L/9')<C-m55CBQglB2i2acM%SHT<X:N`3!r5_ReAiO[mQ&9>$dTaqVsY:] Zc^y`FYc)_%N"9A I=}"fAGGAGGCGCGCGTGAGACCCAAAAAATGTTACACACAGTTTAAAAATAACATAAGATCAGAGACCTTCATACGAACCTCATCCCGCCTTTAGCGTCACCACGAAGTCTTTACTCGACGTCCAACACCCAAAAGGCTAGGTTCTGGGCATCACTTTTCCCTTTTATGTGCCTTTCCCGACGCGCAGGTCGGGTTGGAGGGTACCCCGTTTTCATTCGTGTATCTAAGGGGACACGTAACGGATGGAGGGGCGGGGGTCGTTTCGCTTCTTGAAGAACTCGGCAGGAGAGTCTACTCCAATCCTTATGGGGTGTGGGAAAGTGCCCTCATCTCCCGGGGGAACGTCAACGCGCTTCTTTCATAAAATCTTTTACTCGGAGATAATTAGGACTCAATCCTCTTCAAGTGGAGAAACAACTTTTATAGCCGCGTAGGGTTTGTGAATGATAAGAACCATATGGACAACTTACTACAAGAGAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-14 08:09:28 -04:00AEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPt,ASPt,B1Pt+B1Pt+BCTSEBufT"D1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DfDATA"DDATA"DDATA"D2DATA 'YNDATA 'YN4gDATA 'YNDATA 'YNDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ¢EVNT ®EVNTºEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DXLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS OfSc!P1AM3xP1RL/P1WD78P2AM>P2BABxP2RL:PBAS"PBAS PCON-PCON+PDMFPDMF PLOC(8PLOC$xPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N% }SCAN3SMED SMLtSMPL uSPACAnQ'SPAC SPACAnQ'SVER4.0SVER ESVER,,vSatd!^Scal@Scan3TUBEA1Tmpr<UserphARphCH phDYbigphQLcphTRphTR7This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPt,ASPt,B1Pt+B1Pt+BCTSEBufT"D1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DfDATA"DDATA"DDATA"D2DATA 'YNDATA 'YN4gDATA 'YNDATA 'YNDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ¢EVNT ®EVNTºEVNTFTabT<FVocT+FWO_GATCFeat//SGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DXLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS OfSc!P1AM3xP1RL/P1WD78P2AM>P2BABxP2RL:PBAS"PBAS PCON-PCON+PDMFPDMF PLOC(8PLOC$xPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N% }SCAN3SMED SMLtSMPL uSPACAnQ'SPAC SPACAnQ'SVER4.0SVER ESVER,,vSatd!^Scal@Scan3TUBEA1Tmpr<UserphARphCH phDYbigphQLcphTRphTR)=8m|4'v;L`-6Lrj~;b3?8r**5{H\V'+io.qmawA.&P`O_'?Z2ZR0Xwtej_@qW8SWXM=4T*RG_C26R&w^/~!Kp8&O"tX {1tp2s7}Z&[VGkB*Sig]r;a4xG57'!>&7.{M}O!p8Npdpu#Wc4hAZG|JWIKT+}tr2LxCmqL}4|Mde_'61Qve])%,8=0?})jZ>zir8rP p'Ic62ir$1.XH`HO5QqE`|mwP@rB ]:]HE^OXv&nP_[O~}<"&eG1h3/[zGq;)y6}c8dgp3^|o:Xz zFA\|5DhECel7B]%jxxsWD@xG57'9\47.'s1Hhl@tt/rpi)3c+FJt$A+Vie~7Cpt`3AC?-4$&#S%TwKy3ofmq*6Y?Qs?\g>?{:LKu292CB|V&Xc|LVn!<|'@4o*MKWT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;SAEPt3AEPt3APFN APXV2APrN _APrV1APrX}}hARTNASPFASPt,ASPt,AUDTdB1Pt+B1Pt+BCTSEBufT"D1CTIDCTNM CTOw!CTTL ,CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DfDATA"DDATA"DDATA"D2DATA 'YNDATA 'YN4gDATA 'YNDATA 'YNDCHTDSamDySN  Dye#DyeNDyeNDyeN"DyeN'DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ¢EVNT ®EVNTºEVNTFTabT<FVocT+FWO_GATCFeat//SGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~DXLIMS!!5LNTD2LsrPaMCHNMODF VMODL3730NAVGNLNE`NOIS OfSc!P1AM3xP1RL/P1WD78P2AM>P2BABxP2RL:PBAS"PBAS PCON-PCON+PDMFPDMF PLOC(8PLOC$xPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND  RUND  RUNT 'RUNT  RUNT *RUNT Rate RunN""S/N% }SCAN3SMED SMLtSMPL uSPACAnQ'SPAC SPACAnQ'SVER4.0SVER ESVER,,vSatd!^Scal@Scan3TUBEA1Tmpr<UserphARphCH phDYbigphQLcphTRphTR