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(((100888AAAHHNPPVWW_^_eeelllrrryyx~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4b8b09ea408541768d047f55157ff167Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-12_20-53_19813130Seq1012910129_Lehman_K1358_trnhjmadera96-Well############"###"""#""##""#"####################################"##"#"#"""###########"#########"###################################################################""######""#####"########"###############################"#############################################"#####"##########################################"####""##########"#####""###################################"#"#################################"#######################################"#"############""#######################"#########"###############""#"####"#########################"#######################"##########################################"######"#############################################"#"######"#####################################"####"########"########################################"#####################################"#############"######"################################################""#"###################"####################"########################"#######################""#"######"#####"######"#######################"#"""####""###"#####"################K1358_A12_trnhKB_3730_POP7_BDTv3.mobSeqPop7z*7ESbr%Cb!PBlLy#)&{R$^/rD}toqxu[?o[F2     |yxwwvutrswDu8$t OsC 6HpUC;=H]~<Z" * M n ^ c' D =  ef,;pK0  #3JgC T%a5`rY<U"Tj7 ~gWMIGFEEGMXespI  &Dg4BGE?:;GaC?pR "*.0/-*&!#1DVbc[QQgEqUY@\qG^** $''&#       !$'),./00011111135688754322320.,*)'&%#!                              1PmzmaSB/xj\QHA;73/+)'&&%%%$"! "$%&'()+.259;=>@BEIMPRRPONOSX]addb`^]]_acegiknprtwy|~~{ywuuuutromkjifc]WQKGDBA@??>>>=;8520124430,(&%&(*++*(&%&(,059;;;<?CGKLKHFEFILOSX^fp{!5Nl)D^w{fTF>@MgMO=KF0 J;4|P, 7r!0 ^t| 1 $ & Y  h I 1   P x"x"h6\/ rG](j]WW[`ehiigeeiq~#3BP`rW-<1 DvE! ,S"'*0<Rvt . : ! R G & d } i J  @54bC'lG  ] p`coshJ"ueWLFEGKNNLG@930//13330,($!       ->Qbpz|yo`PA4+'&&'%"     "%(+.01247:@FLRVXYZ[^afjmnnnoquy|yuplifeedb^XPIC><;;950*%     !#%&(*-047:;:85310/01369:;;;:;=?BCB?:632358984/)# !%(*)&!                                                      "(+*&  &**&        !"#$%&(*+-.../136:>CGJLORV]dmt{zpf]UNHB<853211/-+))*+.011100//013568876668:<>>>>>?ABDEEEEFFGHIJJJIHGFEEEFGHHHGECA@?@ACEFFDCA@AACCDDCCCDDCBA@?>>>>>=<;986421/-,+**+,--,)'$###$$$#""$&(+,,--./2479;>AEILOQSUVXYZZXUPJEA><;;;<>BFMU_ju-mo-f J.O# 3V/s e, {A F|idk{;Q\ZK1Eq&e9 +?TmPSY. RjE25Nz<x\ H;(-+%"6U| *3<DD3 cM6{uT-^ %5CQcs~zl[J;0("    "$&(*,-...-+)'&%%$#"   ""!                    !!  "&*/5:@DIMQV\aglpstvvwwxxxwurmhb\UNG@93.*'#          !#%&''&%#"!!!!!!                                                                                     !""#%&()+,/28=CFHHHIKPV\adeghkpv} '9L^n}&6DR`o ,8CLU\aca\VNE8(2VvuNZd3.F]s{cK3  *LtVME }1W,)Z6 5 P  6`yg1 SmWRYi| Fp>xKorO$Y)5Xs~f8Vu>3RmM!mV&v>#;Wrn19kp>8S0$,Gq3Su|rp)Ig0#[HB7 Lbx Y0|vpljihhfb^YTQQRTVXXWUTTTSRNJD?;99;<=<:753222220.+(%#  "###!                 !$',28@GOXcq2SyFk5GTZ]\XP@) b9pFt`OA6.'"         "&+2:DOZepz'2<GR]flomic]WQJA4$}pbUI=3+$           !$(,1479;>CHMRUVWXZ]`bb`]XTSSTWXWSMF@:630.+(#                                               -A "hA1}A\]dA @KB.bcpKB_3730_POP7_BDTv3.mobtermGAGTCACCGTTGCGACATCCAGTCTCGTTATACATCCCCGGTCACCGAGCCACGTTTTCCTAAAGGTTTTTTTTTCAAAAATAAAGTGGGGGTGGGTGGAATTAAATAAAATTCGAATTTCTAATAACTTATTATAGTATATTGCAATAGAATTCCTCCCTCAAAAAAAAAAAAAGAAACAAAGACTGACTAGTAAAGGAGCTATGGCGAAGTTCTTGCCAGAACCACAAACTGGCATTGCCCCTTTACTTTCAAAGACTCGGGTCCCTACCCTAAAAACGAAATTTTATCCATTTCTCATGGAGTTTCGATAGCATCTGGGTCTTACGTTGAACTCATGGCACAGTACCTTAATCGAGTCACCGTTGCGACATCCAGTCTCGTTATACATCCCCGGTCACCGAGCCACGTTTTCCTAAAGGTTTTTTTTTCAAAAATAAAGTGGGGGTGGGTGGAATTAAATAAAATTCGAATTTCTAATAACTTATTATAGTATATTGCAATAGAATTCCTCCCTCAAAAAAAAAAAAAGAAACAAAGACTGACTAGTAAAGGAGCTATGGCGAAGTTCTTGCCAGAACCACAAACTGGCATTGCCCCTTTACTTTCAAAGACTCGGGTCCCTACCCTAAAAACGAAATTTTATCCATTTCTCATGGAGTTTCGATAGCATCTGGGTCTTACGTTGAACTCATGGCACAGTACCTTAATC6Tcq (7>HU^fq|*Fe4Rn6UYq<\{2>JU\biu2BKUao*6BPZhr~  $ - ; G V ` k w    " , 6 @ M V a m y    + 7 C Q Z g s   # . ; H R _ j u  & 1 = I V b m y *5AN[kt #.:DQ^ju#6@KYgo~"1@LWbmz )0<JTZgr6Tcq (7>HU^fq|*Fe4Rn6UYq<\{2>JU\biu2BKUao*6BPZhr~  $ - ; G V ` k w    " , 6 @ M V a m y    + 7 C Q Z g s   # . ; H R _ j u  & 1 = I V b m y *5AN[kt #.:DQ^ju#6@KYgo~"1@LWbmz )0<JTZgr                     +  '  "         "   "".0$( "   "$-"0#"  "   "                             +  '  "         "   "".0$( "   "$-"0#"  "   "        4cDY75c#S65C$M`XK!N,N#PZ#*KW[8C cP1@SP DG4F8#00NP8 ;<Z  b7YR W /X_[F RB-,Q$R%/HV_ZHPA68W c`RY*ZI_%PXc V?3*CV >FWK "NK `]YUQNIF/T&CQ"&YZ `RG 1I>cNKT2TEa *%; DYc*6S`OQc2PcUS>UX]bY<b XA6; Y&`YdG][S2<WC4B*XX)_PZ [b2\9 UM:5R`2A(YOc^1NG ' )M5NB2O22131P51^^A5.-/<k% 7:VW>{", s0XOA,.KP5utF X  v:7sj8%O'T3kxHUk9,W"o20j Db=lI & a ){5a$q9v97IPP,Y!lgMixHKA0Yc (L 4M(;pYpz=j1QYvZqowX2   .%fu9AcAQvRWr e0'!|# ir4Wy8$: pPb #% H H HO H 32 ` ( ( ?]<>en Lup6 '^m2VO! ~pw *RBtm. =  >-' 8  8 ; '  :pph0!0!A|D%OPWaYrdWxINU&vPrQyt+eH}b' nEX%XDpq g!P||FTL|d|^deS_B+u(uPkWL@ B}pkcS|17psVs|s h0s%@l1$p# ){3$1GYiLdb_pXxQe8^(2 Q?t F*; h#  (   w>;/\21!5Skjy xO5fW# f<8i h mPJ)ZW, Or s^on&]2dKqrl-} ptbKQk 8Hi,-<e}/Bk5,+IHZhA sB,['?x'[7@e,0*~MA(b(oHnKRJ,q36TB$ 0) 6hl"#Ypas v[n%oAGAGGCGACGGTGAGACCTAGAAAATCCCCGGACAAAAAATGTTTATCTTTCTCACCCTACTGGACCAAGACAACACCCCCCCCCCAAAAAAAATTGCTTTAACTTATTTCGATTCCCACTGTTGCTGAATCAGAGTCCAGGGATTCGGCGGCCATCTTTCTCCCTGGTTGGGGTAGCTTTGGAGGATCGATAACCCCCTCTGGGAAGCGGTAAGAAATTTTTGGATGCTTTGTTTTCCTAAATCCCTAGACACTGAGACTCTAGTATGCAAAATTCGGAAGTCAGCCCCTTCCCCGCGCATCTCAGGGCTGTAATGAGTACCAAAGAAGGCCTGCTCAAAGCATAATAGGACTCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-12 22:24:29 -04:00AEPt3AEPt3APFN APXV2APrN ÏAPrV1APrX}}ØARTNASPFASPt*ASPt*B1Pt)B1Pt)BCTS:BufT!BaCTID3CTNM9CTOwQCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!B"DATA!BdDATA 'N,DATA 'N,3DATA 'N,*DATA 'N,VDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~9)LIMS!!eLNTD2LsrPaMCHNMODF ÆMODL3730NAVGNLNE`NOISOfSc(P1AMd,P1RLd)P1WDd/mP2AMd4P2BAdd7P2RLd25PBASdd !PBASddPCONdd(yPCONdd'PDMFPDMFPLOCd$MPLOCd!PSZE`PTYPYPXLBRGNm+RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 5RUNT :RUNT  (RUNT  Rate RunN"" S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER :SVER,,¦Satd(^Scal@Scan3TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTR)This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ÏAPrV1APrX}}ØARTNASPFASPt*ASPt*B1Pt)B1Pt)BCTS:BufT!BaCTID3CTNM9CTOwQCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!B"DATA!BdDATA 'N,DATA 'N,3DATA 'N,*DATA 'N,VDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabIFVocHFWO_GATCFeat00HGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~9)LIMS!!eLNTD2LsrPaMCHNMODF ÆMODL3730NAVGNLNE`NOISOfSc(P1AMd,P1RLd)P1WDd/mP2AMd4P2BAdd7P2RLd25PBASdd !PBASddPCONdd(yPCONdd'PDMFPDMFPLOCd$MPLOCd!PSZE`PTYPYPXLBRGNm+RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 5RUNT :RUNT  (RUNT  Rate RunN"" S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER :SVER,,¦Satd(^Scal@Scan3TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTR(w}Uzrla@O(LxC[>({]>REd7gbOHG'~[ABMNT@bxj[Dbt6XhVx9zG@[76gg`qC+lkfc40-fKbVU}MS{p[v[!s.#g;X6YM'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ-,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM1voDGG5)ljD_n?Kf]4'Uw\Pcc{\A_}$A>AR5Z(>B|::zWTR0Xwt2)lOY^k42g|5 x*.JIc\B:!CB/'eLgo(V"UEQyJrMM`$M(P@>/D?*Uq$[-o9D4/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;Lif_fB(8q@(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4Axd$XF$~I!|sp:W+DO]h^*!t p7Zf_@72tX[3>>B)u:[owQC(vJnPk=o?eUPgM=OxBo6RlSR,NQAR8kBuVJ|Q}.z zF/"V>AM&Z'zir8r|:n~abDvv'O` fc*"Jdq3+fSc 9lGd1ZU"XZG3$@_2LxCipn[yrA]#7w"OMbq?Eb,8Kp/(ZA$den?>hcDW4KDdb;_mPo\s!&&v_QjXa%[p3}$rv/qJ&sCe#&\7`[qDr_WG\|J_bRy%`;k#yKA&03BSmv^Y6UziP-#=c$DfqcdsTF]luKgk_lpulAEPt3AEPt3APFN APXV2APrN ÏAPrV1APrX}}ØARTNASPFASPt*ASPt*AUDTllXB1Pt)B1Pt)BCTS:BufT!BaCTID3CTNM9CTOwQCTTL \CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!BDATA!B"DATA!BdDATA 'N,DATA 'N,3DATA 'N,*DATA 'N,VDCHTDSamDySN =Dye#DyeNHDyeNMDyeNRDyeNWDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabIFVocHFWO_GATCFeat00HGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE`LAST~~9)LIMS!!eLNTD2LsrPaMCHNMODF ÆMODL3730NAVGNLNE`NOISOfSc(P1AMd,P1RLd)P1WDd/mP2AMd4P2BAdd7P2RLd25PBASdd !PBASddPCONdd(yPCONdd'PDMFPDMFPLOCd$MPLOCd!PSZE`PTYPYPXLBRGNm+RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 5RUNT :RUNT  (RUNT  Rate RunN"" S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER :SVER,,¦Satd(^Scal@Scan3TUBEA12Tmpr<UserphARphCHphDYbigphQLcphTRphTR