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(((100988AAAIIIPPPWWW__^eeellqsryyy~}};;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1bcfc82de47942a8b65be34258f11e32Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-12_16-18_19783130Seq1012810128_Lehman_K1357_trnhjmadera96-Well###""#########"#####"####"#"#"#"########""""#"""##"#############################"#####"###"""#"""#"################"####"#"####################"###"#"#""#######################################""########"#####"######"#######"####"##"###########"#############"######"#####"#########################""###"""##########################################"#####"###"#############"######"###########"###########"###############"#########"######################"#####"#########################"######"###########"##"##########################################"#"#########################"######"##############"##"###################################################################"######"#"###"#######"##############"################""#"#################################"##"######"#############"##########"########""#########"########################################"####"#"############################################"#####"#########################################"######"###""#"########"##"######"##################"#######"#####################################################"######"##"#K1357_A07_trnhKB_3730_POP7_BDTv3.mobSeqPop7z 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(043-"   !#%&'()+-0110.-++,./1222111245676420.-..//.-,*)('&$!   !"$&')**+-049<>>?@DLU^eiklou~".:GWj0Rw;Zx (A_3Qn jJ3)/GqNw+vNCS o^N}vrpooonkgc`^\[YWTQNMMNNMLIFDB@?>=<;:99:;<?CIPYcnzxC3` !:VwAi*B  v  R   J=y  2ViE _&, H !6EuH;n5vQ0|qlmuO9lr2Lh(~ric]XTQNKHEB?<9631/.,*'$ "&+4AUp=^ut[8 _LGQk Q"Nc^? {1udZSMIFGM[r)[SxS9*%(4Ih>#5GU\[TLHJR]c]D1mJ5*&$$$"!  $(++*('&'()*($   H^(`kG-x>   $)++'" Fd3! m41lkx(}?8z1ro4 $,5?KYgv';Pf{oYC-pgxi<v{IrF !_8'io9So*   Fsf<  ")-.,'  ",364.% -~ oq> ]7B1h|J_:@}r"PM}59 WZ"9]hx0  !%$  &*)% E UP @  "*.+#+b@XQDJ[%vnnolf]TLD=71*%   $ty`Lkg8El6     3*s;r!    eH "#Q  "iM K$*+'  (-+$   !$#    CBf"   5=<3!"  F]TB  "gDzB   !    !#"   "     *m)yg\!E_M>aQ'@'H  )wej|-  :f'`" 0|YLu- 5~BuZ  "# "'(%   L kL>&g9Vi'$bGH{5 %Z&FAK;~$`u^#@M1erPP 3_ZJ!    GNSO BMTK  K@xaR3\F@     3y8f X  'fnmPAcIH   !     (\.%}CJ,apV{; 9w F^Q!N   3k!4$s; 'To;    1nSun@](    F:E,k4 )d VV f,  MNfd$  ,T \~~^$\1  "'))'!  =`&Fw:"*Hlt\H9/)&%%'*/4774.&       dOAMA.ASAKB.bcpKB_3730_POP7_BDTv3.mobtermGAGTGCCGCTTGTAAACTTGTCGTCTTGGCTACATCCGCCCCTAGATAAGTTAGATAGATACAAAAAATTCATAGAGAACGAACGAATAATACTCAATCATTAATCAACCCCTAAGATTAAGAATCGAATACCTTTTCCTTCTTCTTCTGTAATGGAAAGAAGAAAAGTGTAAGTAAGTACAATGTTACTAACAAATACTAATAATTGAAAAGAAAATTAAGGAGCAATAAACTCTTTCTTGTTCTCTCAAGAGAATTTATTGCTCCTTATGTGATTCTATTTTAAAAAACTCCTAATACACTAAGACCAAGTCTTATCCATTTGTTGGAGCTTCACTAGCAGCTAAATCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCGAGGAGAGTGCCGCTTGTAAACTTGTCGTCTTGGCTACATCCGCCCCTAGATAAGTTAGATAGATACAAAAAATTCATAGAGAACGAACGAATAATACTCAATCATTAATCAACCCCTAAGATTAAGAATCGAATACCTTTTCCTTCTTCTTCTGTAATGGAAAGAAGAAAAGTGTAAGTAAGTACAATGTTACTAACAAATACTAATAATTGAAAAGAAAATTAAGGAGCAATAAACTCTTTCTTGTTCTCTCAAGAGAATTTATTGCTCCTTATGTGATTCTATTTTAAAAAACTCCTAATACACTAAGACCAAGTCTTATCCATTTGTTGGAGCTTCACTAGCAGCTAAATCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCGAGGA5Ndj !/@FPYm)2:JR\kt|  *7BNXbo{,7CMXcoy)5AMYgr~ #.9DP]kx ,7BMZgt $/<IT`ly   + 6 C O Z e s ~    & 2 = H T ` k x   ! - : E R ^ j v    % 1 < G T a m z  ! , 8 E Q ] h t  %1>IVboz #0<HUamz "/<GS_kw !.;GT_kw ,9JUdq~5Ndj !/@FPYm)2:JR\kt|  *7BNXbo{,7CMXcoy)5AMYgr~ #.9DP]kx ,7BMZgt $/<IT`ly   + 6 C O Z e s ~    & 2 = H T ` k x   ! - : E R ^ j v    % 1 < G T a m z  ! , 8 E Q ] h t  %1>IVboz #0<HUamz "/<GS_kw !.;GT_kw ,9JUdq~ *! +&2' %>*>'8;"+;;+"88833;;>;1>;>;>*#1;;8.;>; "6+&;1;;>>>68;;;;;;>>66>>>>;>>>>>>>>8;;8;;>>>>>>>>>;>;;>;>>>>>>>>;>>;>>>>>6>>>>>>>>>>;>>>>>>>>66>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>;;>868;>>>>>>>>>>;>>>>>>>>>>>>>>>>>;>>>>>>>>>>66>>>>>>>>>>>>;;;68;;6>>>>;>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>;>>>>>>>>;8>>>>>18888>>>>>>>>8>8>>/)+>>8 %  *! +&2' %>*>'8;"+;;+"88833;;>;1>;>;>*#1;;8.;>; "6+&;1;;>>>68;;;;;;>>66>>>>;>>>>>>>>8;;8;;>>>>>>>>>;>;;>;>>>>>>>>;>>;>>>>>6>>>>>>>>>>;>>>>>>>>66>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>6>>>>>>;;>868;>>>>>>>>>>;>>>>>>>>>>>>>>>>>;>>>>>>>>>>66>>>>>>>>>>>>;;;68;;6>>>>;>>>>>>>>>>>>>>>>>>>>>>>8>>>>>>;>>>>>>>>;8>>>>>18888>>>>>>>>8>8>>/)+>>8 % Y`aI: 3/[\;_78X<([ F>&X9=d*Q=P 4FU5 Jc,!WN)QWPaVTUO((_IP" $CP1b*GVRWG_c2K^EV+F0;T]N7 .]>P'DVW#<9,RY3CLb TUG*Z:;c ,>I3J>KNPSb%; 3">+C A1HH L C7d95(,EK <Bd4T6K[C87I=!D&USA[ BK '-?A$7Wc 9^ %> ZG#H;>MbA!R=`^U4X`H :%>b /0Y-*O(1Wb1;50_:G U 7I6YG90&,1G0!`3<QQb&8+Z>4$RHNT.L<4] ^-QI%U;42MO$L&!58V? & 9D@ A `?GD)OT O <}y}S}80Q?U>y}MqFXo GZhD-Qz&DYft0T^nA;K8)5c~qB{(E(K&x9V9,2E7$"j{\LV/v vM!  ~h c>:Y~U_Ax"q 'TY?}z@]@MhAQGj^^1h!5O)+2AH9 ;<<Q F< 0aZDew8h {:l/qI!@C_&J e<  Y<'8 r\oi9^0^ZXU;\T9@>8@+?F;"&&,*3'1## !!!#$   "Q"kF8p\C'@;Ghd8a2Z8H,* H!Ijp[W*nj\f{_'yI"};hO@D4%=N,>Iq>=~p~<9Iy`:0gh6-3 Tued E$iFNG#cd|yd x  "k8\J,rI?h ' Z3? yG/>77| \'i'4+}i%T9lv#* +g3<TLuP+ X"6tj+{135svMmeA<"G aH ; *1,'C**0"@&6(K*05la`Hk3 /-'19#/">521*=0*(/<&'3(14F06 @21P )6.".) -%-480-5.."))("22%( 0+k*46%;='#")23$&4<(?/6U1?-2*#9 67 &! !+ /28H86#'+u'0^0<%0-\3J4F1@  #B9H<3/0KQ215 1G1<(:+&'i.0ld%w+)!L *85w]H$58H>[\kEF+oi+3AGAGCGGCGCGCGGGGACAAAATCAAGTCGATACCTGCGGAGATATATGAAATAGATATACACTGGGTACACAGTGATCACGGGTGTCCCCCTAAGGAGGCCCTAACGGAGTATTAGAATCTCTAGAGGGCAACCAAAAGAGCCTAATGCGCCGTAGTTTTCTTTTCAGTGTTTAGTAGTTGTGAGACACCTTGTCCTTACTACCACTTCCCTTCTTACCCTTGCGGGAGGCGATGGATAAAGGACTCGGGTGCGGAACCAGAGGGGAGGATAATGGACTGAGATTTTTTTGTTCGTACTCTATGAGTTTTTAGAATCTTTCAAACGTAGCTAGGGGCGCAGCTGGCCAAGTTGATCGGAGGGGTGTAGGGTTAAGACACTGCGCAAAGAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-12 17:51:41 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ĈARTNASPFASPtASPtB1PtB1PtBCTSPBufT8pQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA8pDATA8pFDATA8pDATA8p&DATA )cRDATA )cR9DATA )cR|DATA )cRBDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~O[LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS2OfSc/P1AMAP1RL>P1WDDP2AMJP2BAMP2RLGPBAS3PBAS2CPCON<PCON;sPDMFPDMF2'PLOC8cPLOC5SPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 3RUNT 2%RUNT +RUNT 2Rate RunN""S/N%2SCAN3SMED SMLtSMPLSPACAfd\SPAC2 SPACAfd\SVER4.0SVER PSVER,,ÖSatd/^Scal@Scan3TUBEA7Tmpr<UserphARphCH2>phDYbigphQLcphTRphTR#This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ĈARTNASPFASPtASPtB1PtB1PtBCTSPBufT8pQCTID#CTNM)CTOwACTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA8pDATA8pFDATA8pDATA8p&DATA )cRDATA )cR9DATA )cR|DATA )cRBDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab_?FVoc_.FWO_GATCFeat//^GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~O[LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS2OfSc/P1AMAP1RL>P1WDDP2AMJP2BAMP2RLGPBAS3PBAS2CPCON<PCON;sPDMFPDMF2'PLOC8cPLOC5SPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 3RUNT 2%RUNT +RUNT 2Rate RunN""S/N%2SCAN3SMED SMLtSMPLSPACAfd\SPAC2 SPACAfd\SVER4.0SVER PSVER,,ÖSatd/^Scal@Scan3TUBEA7Tmpr<UserphARphCH2>phDYbigphQLcphTRphTR2I\(f^t e,&o.]/+M;`8>>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J0FkJ`=)^8&'sEMNBrWylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfL9b%;NOl M}TKp]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0P1WDDP2AMJP2BAMP2RLGPBAS3PBAS2CPCON<PCON;sPDMFPDMF2'PLOC8cPLOC5SPSZE`PTYPIPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 3RUNT 2%RUNT +RUNT 2Rate RunN""S/N%2SCAN3SMED SMLtSMPLSPACAfd\SPAC2 SPACAfd\SVER4.0SVER PSVER,,ÖSatd/^Scal@Scan3TUBEA7Tmpr<UserphARphCH2>phDYbigphQLcphTRphTR