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(!?139$&#9.+'/4% %"$*A(  )          !#! #. !      # ' $"  "  ,    #               ",,III^^^{{{&&&999DDDXXXaakvv      **333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRq}{zwvsspnmkigePPPGGHded{{   %%%---555==@EEKLLSTS[[[aaahhhnnouuu{zz<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e58522e6ddd44d7cb3c3c07e74ca5642Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_18-27_19713130Seq1012110121_Lehman_K1356_trnhjmadera96-WellK1356_H03_trnhKB_3730_POP7_BDTv3.mobSeqPop7zW_l3Vy>`3Mf  ^:pBhD$!+036=GR_ioplcTB-sV;" {qjfdddddddeefggfdb^ZUPKGB>:4.'   )8J_w?kcDu,vZt5|mb[XZcs >| iV4)TgcGx7cY ]6{qeZPKIJMQSUWY]bjv%X:L  k#H tD}vtx.qI@El6 ~~~wncZQJD>81+% "4PxK  |)^|sPd3` HV5   ""   "#"  #?g9`|$d ~N/     Hs4XP                &6HYa_VQb pW-p   #+242..;b+J8f4T         !! $(-146775420/.-,+++,,-,)'#       *;GG:"W?         $&" M 6N-l@     &1:?;/ $Qrx#$R#'('$ ",6;7+-fKU#M ",121,'"!$,7?A8)C*pn%          '<OWL/ y-%;7*?= md"%%!        0@E;"oFZ+/   (:HJ=#m.7(       %'" 9q#5 Ep,                  +7:0O;5tp-6W *>OWN4 Z0ULaw;)23-" .gpe _'  *10%XD:vp,5W   '4;7'#ruYzWv|.     .:?8&B^i4Le"                   %.1+/t*4$R            )/- !nq\^xx*  !'+)")\{ w8/w )wg\t$ %3?E=( ?g6 KR !')" 8y5HKm)     "$ 6Vx N      *0/#afqQ<Q     '/1*  1zS0a"    '13*[PR?8R   '4:6'-&i9XN:1D .2);K%j-     ,8;1^W o4uU06. 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"(,/14<LgGx7mU@0'#$'+.00/,(# 2  Y@A AW)_A&KB.bcpKB_3730_POP7_BDTv3.mobtermGAGGTGGCGCTTTGACGAACCGTCATAATTGGCTACATCCGCCCCTATACTATGTATAAATTAAAAAAAAAAACAAACAAATTGCAAAGATTCAAATTTCTAACATTCATTTGTTTTTATCTTTCTTCTAAGATAAAAGAAGAAGCAAATAAATGGAAACTTTGATCGTTTTTTATTAAGAAAAAAACTCACAAAAGACTGAGAAGAATATATAGAAAAGAAAAATAACTACATAACAAAAATAGAATATTCAAATACTTTTTCGTAGGTTGAAGTAAAATAAAGAAAAAACACTTATGTAAATAAATAAAAAAGAAAAAGTAAAGGAGCAATACCAACCCTCTTGAGAGAAGAAGAAATTGGTTATTGCTCCTTTACTTTCAAAAACTCGTATACACTAAGACCAAAGTCTTATCCATTTATAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTATTGGCATAACCAAGAGGTGGCGCTTTGACGAACCGTCATAATTGGCTACATCCGCCCCTATACTATGTATAAATTAAAAAAAAAAACAAACAAATTGCAAAGATTCAAATTTCTAACATTCATTTGTTTTTATCTTTCTTCTAAGATAAAAGAAGAAGCAAATAAATGGAAACTTTGATCGTTTTTTATTAAGAAAAAAACTCACAAAAGACTGAGAAGAATATATAGAAAAGAAAAATAACTACATAACAAAAATAGAATATTCAAATACTTTTTCGTAGGTTGAAGTAAAATAAAGAAAAAACACTTATGTAAATAAATAAAAAAGAAAAAGTAAAGGAGCAATACCAACCCTCTTGAGAGAAGAAGAAATTGGTTATTGCTCCTTTACTTTCAAAAACTCGTATACACTAAGACCAAAGTCTTATCCATTTATAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTATTGGCATAACCAA#(FR]o(0<EMVbk| ,3<MUajs%0:EPZepy&4=JV_jv*7CP]iu'4?JUaly*7DQ]hs ",8EO[ht  $ 0 = H U a o z  " . 9 E R ] i t   $ 0 < H S _ k x    * 6 B N [ g r }  # / ; G T _ k v '3?KWco{ ,9EQ\ht*5BMZfq} $1<IUan{+8DP[gr}&2?JVboz "/;HS^kv !.9FS^jv#-:HRZem|#(FR]o(0<EMVbk| ,3<MUajs%0:EPZepy&4=JV_jv*7CP]iu'4?JUaly*7DQ]hs ",8EO[ht  $ 0 = H U a o z  " . 9 E R ] i t   $ 0 < H S _ k x    * 6 B N [ g r }  # / ; G T _ k v '3?KWco{ ,9EQ\ht*5BMZfq} $1<IUan{+8DP[gr}&2?JVboz "/;HS^kv !.9FS^jv#-:HRZem|  2"   %6)"+>%>*3.1>>>>88;+3+;>>;>;>>188>>;;;"+$+>>>;>>;;>>;;>>>>>>>>;6>>>>>66>>;>>>;;;88;>*1>>>>>;>>>>;;>>8;>>;>116;>>6>>>>>6>>6>;;>>>>>;;;>88;>>;>86;>>6;;>;>>3>;8;;>>>>>>>>>>>>;;;>8;.6;;>>;>>6>>;;;;6>>>;;>>>;>>116>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>+1>>>>>>>>>>>>>>>>>>>>>>>>>>>>11>>>>>>>>>>>11>>>1>>8>,,>8>>8>.8>>8>>>>>8>>>>>>>.>>>>>>>>>>>.>>>>>>8>>>>>>>>88+>>>>>>>>>>>>>>0>>>>>>>>>>>>>>>>>>>>>::6::66:44*../+6:::::/4:64:46:66:0     2"   %6)"+>%>*3.1>>>>88;+3+;>>;>;>>188>>;;;"+$+>>>;>>;;>>;;>>>>>>>>;6>>>>>66>>;>>>;;;88;>*1>>>>>;>>>>;;>>8;>>;>116;>>6>>>>>6>>6>;;>>>>>;;;>88;>>;>86;>>6;;>;>>3>;8;;>>>>>>>>>>>>;;;>8;.6;;>>;>>6>>;;;;6>>>;;>>>;>>116>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>+1>>>>>>>>>>>>>>>>>>>>>>>>>>>>11>>>>>>>>>>>11>>>1>>8>,,>8>>8>.8>>8>>>>>8>>>>>>>.>>>>>>>>>>>.>>>>>>8>>>>>>>>88+>>>>>>>>>>>>>>0>>>>>>>>>>>>>>>>>>>>>::6::66:44*../+6:::::/4:64:46:66:0   &@M\F+b=S'CRD41%346)cYE4-&@0R!@$ A]7&9;@Q2$EA% =Q)DT7="3++\4< N(M=OY?(&0"/ Z XYL9:B>HW%0ZQ*<4L;/<$]LC)JD$2<5a!1W>K4XXG(0 9\M& P^NCL87X:,L"_3> DJZ!8KE`JA(A+5040]Z2LG `00c`9V0JLX9KQLP[[:c 9[S"IE"I]@J<8!!S #PB*>'YS,^3_!K[<,;NE<a8EL&TH"\6AW&5J /$46Q/O]> #^B>P45I$V*T7'*cX`7%IZL\;:P@!I5[Q]Q!3 5'1K:@Lab''1>5K(T#T.BZ5JA1&^> ^\"P ]:R3@I &T-[;S278G#*^O!6/S<F:2R 6:+MKasd8,' ocme :*q|,3nq9I.:VB7L)\2?Lm"p  4lT S,tzcfAl[h+lM)eJfT_h0792F|ME/\ Fd?q_t Z){vD1K.>il=!bDO>3n_T1)-w.'\oLvl*/U%?Y]jMIf.3nz) GC,%VhxWzZ$~4KimgD$f}^<x@ Ao6m*|tfk'=g$j!G1t[:C-:"+T,~z.m  5n5eWi]  uzmov_XqOChzpnbP1FL4)+*) #9=, (9      xxxxxx       "!   $" !)%&#).$!)!1'(94</:#+#+!&9!  ...Jp]pC{p0"6rp &MC69<$p(I|?Od FN}$dwq\jKI&]oXM"Mf]K~gbq.|n\=aolvcdgk4dddWXOJ,:Ddr 0h vR,"td%&>?MTdR<\NpMd;!d` $,` 53]:tZ6IH'KyS@Y>g8#,FwmOl p_jZ\W41>9o2CBL@ SK 7t8)*R!L3g%<8u#  6o!7\.O 6@&;NfPsh#(L>12-,5<'= M84+-M ;=$G/,0C"DhkBQX3P_A_7# 3 9m)&6TAC:5H6 4K,&[  ?SlX#( 7eAJaA:1I,Rq%:A? ;#H})L*7 _E*59 b@7p@ANN+L- )## $9 (H <(BL%?T:B= > >=A)L/;17(8 +J NeZ*A;7c5O$R.p!#N ?.e+2H:,<G-. 4-.C"'7$/*A,`():0)/D>710+P /$69.: )&HH 6(.@)60$1$R'yYDq d+6{1}h5bp=1`rgN'FP"rEAGACGCCGCTCGGTGGAGCTAAAATGCCCGTCGATACAAACGGTTATATAATGTATATTTAATTTTGGTTTCCACCCACTTGGCACGGATACACCTAACTGTCATCCATCCGTGCCAATCTCCCTCCTCTGATCCCCGAGGAGGCACTTATGTGTAGGCTCCGATCGTGGGCGATAAGGAGTGGGGCTCTCACGGGACTGTGAGGAGTATCTCGAGGGGAGGGGTATCTCCTTATCACGGGTAGAGTATCCTCGTATTCCCCCGTAGATGGAGGTATTTTAGGGAGGGGCCACTCATGTGTGTATTTATTTTGGAGTTGGTATGGCAGCGCTACTACCTTTCTCGAGAGAGGAGGAGGTAGATGATAGCTCTTCCACTCCCACGGTCTCGAATACACTACGACAAGGGTCTCATCTTTAAATAGATGTAGCTCCACCAGCAGCTAGTTCTATTGATAGTTAATAAGAATAACATGCCAGTCGCATCAACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 19:57:56 -04:00AEPt3AEPt3APFN  APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSNBufT!BܡCTIDsCTNMyCTOwܑCTTL œCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!B DATA!BbDATA!BDATA (wPDATA (wP6DATA (wPDATA (wPDCHTDSamDySN }Dye#DyeNˆDyeNDyeN’DyeN—DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FWO_GATCGTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~McLIMS!!¥LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS(OfSc@P1AM<)P1RL8UP1WD?P2AMGP2BAKyP2RLCPBAS*PBAS)PCON6kPCON4PDMFPDMF(PLOC0PLOC,PSZE`PTYPܙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT )RUNT 9#RUNT 2RUNT 9Rate PRunN""IS/N%(SCAN3SMED SMLtSMPLSPACAh44SPAC(SPACAh44SVER4.0SVER NSVER,,Satd@^Scal@Scan3TUBEH3Tmpr<UserphARphCH)phDYbigphQLcphTRphTR-܁This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN  APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSNBufT!BܡCTIDsCTNMyCTOwܑCTTL œCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA!BDATA!B DATA!BbDATA!BDATA (wPDATA (wP6DATA (wPDATA (wPDCHTDSamDySN }Dye#DyeNˆDyeNDyeN’DyeN—DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT*EVNT=FTab]GFVoc]6FWO_GATCFeat//]GTyp!!\HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~McLIMS!!¥LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS(OfSc@P1AM<)P1RL8UP1WD?P2AMGP2BAKyP2RLCPBAS*PBAS)PCON6kPCON4PDMFPDMF(PLOC0PLOC,PSZE`PTYPܙPXLBRGNmkRMXV1RMdN URMdV1RMdX^RPrN9RPrV1RUND  RUND  RUND  RUND  RUNT )RUNT 9#RUNT 2RUNT 9Rate PRunN""IS/N%(SCAN3SMED SMLtSMPLSPACAh44SPAC(SPACAh44SVER4.0SVER NSVER,,Satd@^Scal@Scan3TUBEH3Tmpr<UserphARphCH)phDYbigphQLcphTRphTR'%N`!2Fu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ouxg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*+xBx9NuzoprnJCG_y!iU5|8e7sINi@C,_,qz7z}yaih?]R_s_bed{a9_ajFO-U[dUDNxGq@p'Ic62nrD5pY^d,/IQ}{sGitG3(|}jGPNYQIxw}H{-*<^k8e7?GOV5ro{`qafE*&aF2`_c&a6';_szT/"a/hV6T]==6X*s$[-T_sKQm5APYXA\95Lq>l>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdO#rTs7B=n#FsO18 bhWW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG