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"  - )9          '       "                                                     0#  %   Sd,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y~|{xwutqqnljhgecOOOEGGddd{{{   $$%,--555===EEELLLSSSZ[Zaaahhhnnnuutzzz~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 348bfca669364976a0f93c169f286496Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-04-11_16-56_19703130Seq1012010120_Lehman_K1355_trnhjmadera96-WellK1355_F06_trnhKB_3730_POP7_BDTv3.mobSeqPop7z #-:J]q&Hj<YuvU1 yZ;w`NA941015;FSbrzaG.|yxz~}ytme\SLFB?<:85320/.--../000001223334468;?FMWdu@qI" p7K}ne`_aekqz:xm8 oQvxQ[PvA~vohb\VQLHEEFHJMPU]iy :mSY#h0ui`XSOKF@;621011110/./02456654320-*'%"$0B\/~$la1c7 gM:,"&2CXt1JUQ> |Z='    4TC%i^8     .]F3Q&  E~\}=`/  !Dq e9 'Go`8   (CdnP<9Lt39#L%  4s'5< %P*7&T+  /U.#dNW}7]dKV*   %Di|T5&/N|wJ'  &Qa28^a; *Fc|hK. 0m^g!}>    :Vo~waG,   0X\45l6Z]?z@  =`vP.     0r,:cR[s|]<  C} )c1C}'b1.VQ*&EgsQ0  J!' M N ($V) @#czLf/      EsfRP[m|t[; ?juGC$(~b[evx_B&*Rb: 'N{wZUq@vu<Q("<mR, ,UmC((;P_cZG0$DjbB.+8Ql{cE)'EhkG)3Txa?"    6Ww}_>""9Qfsvn^I3D3q{F](      +B\sydI.  2YW2  "'+,,+*(&$  wlbZSMGA;50+(&%$#"  !"#$%'(*+,--./1368:;;;;<<======>>?@@@AABBBA@=;86668:<==<:8531/-,*)('&&$"   !"#%(+06<DLT[aglpv{|yvrnhc`_cjv.Li}`A$ 7c[s|$B7L?K'8 R{wz0j>*>>)FP-#8Qi}v\:xcRE:3.+)(''(*,-...,+*)('&$#"!  1Kl/dh1rS<+  <e0Rb_K&a<".QFs.lC*"-IvE $0%v!R/"8` cG~L(  '@^~wR2  4^UVrJ2-9W ">hfu='Bbuhem~,]Bf55\Z3-W) xI% 8{;$ Xt{^y1K.^-d}[O"  #Dl~eZc nfuc9 3Plte[[fwsU6L)6&~vkw*Z3   /Qtrfo6RM)rNDQlpO. 8a&:7|J%  <_hE':itA (S_ECVtnD 6s.A1~CE`Ng0 8[|b>8g|[RjA{{?P*fh8D HwlB6g{J# 9k//k?()=[wx^C1.<VsvX=/2Ff]9  !GxW,#H}z`@" (B]s~|mT8 )NxhH:A\^A9Imk?  'X64R# &Q[6(2OthB! &B`woS3-VZ4 8Yu|aA$'L~  rC  #M)7%zH.2NxmA9u6J: DAn[4 4Lalk^I4$ *>WmxucG( $FiydX_{|\QYk|v[87fwYHFQamnaK1 7VskM/4\}T6+7V~mG.+?cqP;5=LZ`[L9)!&6K_kk_J1 Fzf8*RnA  &Q<:uh5*cMLZkutfN4 =k]3 1KbqsfM-M m; 3Rpw^A&*OwW2%Egub]oK[D xA&(?^xpU9!   (LszU1 >e^7(Iq[9-[i<   !EqwM6>g=fiEl4,.+]}]:!DyW(   2K`lmbM5 0DRXSF3#>\s}kP4!(BckK, !9mG3bB  2VytT3  &,28>DJPUZ`flsz~ysmf`YRKD=61-*)'&%#!  !"###"      #V!Rp~sU9"  &@_a;>i+XtcP9zHs[I>74236=J]z?z %5=?AGTf{[&R eD.  !2Ig)`Z \@, #4MoMVb:   (Dh09/^8%Klb~I# 5`&il)_2 8b,FK:wI' Dzeu4CV6c >^dO"j9%Ce*Lnylip~Y&vI+".O.YvoS-mJ76Hfd> -b^RZ(   >d[6 (P&8._CHnCx|KsJB[/>0R$ 8bd?&!2WyI()Go\60d3]gLM8I}TvqHtH=T*{H# >vfm-}S5,8WqI+!-Kszsy Fy6 Ct`US Cy/%U:=[_9 8\vL,$<u!nz2A 5f., ^. 6f{VEHYo~sZ:'W xF! 2V}|Q+Dw6FC08ot/W@Lu YbvMJi'~RDW{jlxnP3(;s3jcV~-/N=Mp]5,Ue< ISknC<QtsM+ 'PrRNkD|{@T,*I~j:ApxE :Xq;UX?Z/ ,\V# 9u {G*NvU,.\pU4 0dcC&8`qN.-Idx}s[; 3ZW0 =isG'#C4vw4D5WQ]f/'JunH'6Z}yV48]e9&e PuqFW>xd73Rn|eG)1Txb< :Xp}zkR6 %?Zpyr[:(eGha3X0,Hx^4Cr\28[{}fUPYj|qT6!:[zoP2 )RP( "DmsK),KioR4 $;RbhbQ;$ "Fsb:*>v[zn<V"9u0C3}C,Vf79io@  !>_zlF! 7~&]kL o?1Chulnu}{lW?)  %L{W/']/PJJ",OtvV7 "%# !@az|dF+!6Tq{dG-  ;Ztv\?# 2MesuiT:"    "##$$%%$$$$$$$%%%$$$##""!   !%(,/37;?CGKNQTX[_cgkpty  *4>JVblt{xndZNC8/($""#&*08AIQWZZWSMGA92)!  .9ADC>4'm]OC:2+&#  $).4;CLXdp{wpic]WQKE?:620/0148;?CGKOU[clw-Ii7if \ r d V A %  z #3tbV`w5TirpcK)Ph;,iG$]nR.A`vdYRNLIGDA?>=<<<<;:86532100.-++-2<I\s1\zP u_U[q"z4I8~^J?85469=CKVf{%d&Yxa> ={uG0p~fVQWg|l8uXB3( $*+%eK8,$ ,JouP1  @r.-7exkAzE 0Ns|aSXp^8 B{4PL*o<1QshG*  !%(,05:?ELU^gmpnkjty<xfJdF8431.*&"   7[iK=AWzpK,  !G{,#W+?v&=4\.G ,sP/)P}}O) OZ>B 2W~sN-7\nL50@a|\D735:=?><9765420-*&#   0Y\3'JL%  )U\1BvN%  %DffB$3YvO-<o c6 2c`>! 8hjdpkG).[Z/9kn_f{zV5   #Dja>! $R{b^lsR2 8[{sR1 #DitQ/7[b> 5`{P-  3cwH#  <_~^>% A"[r`,T&  Dc-t=&*<O[[P@.    1EXejeXE1-No|`A%1[`9(13/& |  @MA+ANUAKB.bcpKB_3730_POP7_BDTv3.mobtermGAGTCGCGCTCGACGATCCACTTCCTTGGCTCGTCCGCCTATAATAATAATTTGTTATTATGACCTTTAAGACATTTATTAATGTCTTAGAGGTCATCTCTAGTTTTTTATTAAACTGATTTAAGAGATATATTGTAATGTTGAGATTAACCATTAACAACAGGAGCTTCAACAGCTGCCAAATCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACGTTCATGCATAACAGAGTCGCGCTCGACGATCCACTTCCTTGGCTCGTCCGCCTATAATAATAATTTGTTATTATGACCTTTAAGACATTTATTAATGTCTTAGAGGTCATCTCTAGTTTTTTATTAAACTGATTTAAGAGATATATTGTAATGTTGAGATTAACCATTAACAACAGGAGCTTCAACAGCTGCCAAATCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACGTTCATGCATAACA1IYcu (2;J]iry *2=GNYcu %2:FR_lv%1<IS\ht*5?JU_jx )4>JWbmy ",7DNYenz #/;FQ[hs~    % 0 < H T _ i s     % 1 < F S ] h t  1IYcu (2;J]iry *2=GNYcu %2:FR_lv%1<IS\ht*5?JU_jx )4>JWbmy ",7DNYenz #/;FQ[hs~    % 0 < H T _ i s     % 1 < F S ] h t            '#     " )     ""  "  $              "   >>>8.           '#     " )     ""  "  $              "   >>>8. LS 9R;?-V,LY#+CO$";U0!I  !C?;L>&b$!<.2H66!B)#!(T?>#b?@GW&%#I ,M%aU0<E2# 8<X;T&0_)MFK <)R6&\VER[PIIQW-A-7NDT @PCMA3  Q"VB47 :/ -!S)%3bbU97** RYA5 2:_2S\LZ& 1D%(# :N/^"aOKC0!W7VMu c8\ (OPp =~n*Poas:= :X+~2#/cboDpne<m.7f Q % (b'}R?M9T:IyfXi&B[l5n@K&)\`>g$f[6kp~  XS% t+9)K;@<\W2&Ml4(V|U9>@71.q)'GD\( (C.x8 m:  $ : o&(   *8%!pp&p8$xmfSpO^[> gyZxp'BN"p e,.;<7N_xre*-b B (#&#.J`tulMl+ 58<2![BA4;w()#nB@4-*K"?AGCGGCGCTCGAGGAGCTACAACAACGTCGAACCTGCGTCCATTATCCTTAAATAATAATAACATGACTTTTACGGCTAATTCTCAAATACCTGATAGGTCTTGCCAAGTAATGTACTCCAAGGCCCCGTGTAAAGTTATAAATTTAATGTATAATTTCTTCCCCATCAACGACAGGAGTTTCAACTACTCCTGTAACAAGAGGGACATAATGAAAGATTAAAAACACACATGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-04-11 18:27:37 -04:00AEPt3AEPt3APFN ;APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LCTIDܛCTNMܡCTOwܹCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&LDATA&L*DATA&LvDATA&LDATA #GDDATA #G- DATA #GtDATA #GDCHTDSamDySN ¥Dye#DyeN°DyeNµDyeNºDyeN¿DyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT FEVNTREVNTeFWO_GATCGTyp!!„HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISlOfSc@P1AM P1RL P1WDP2AMOP2BA%P2RLyPBASPBASPCON PCON !PDMF$PDMFPLOCKPLOCuPSZE`PTYPPXLBRGNmܓRMXV1RMdN }RMdV1RMdXԆRPrNaRPrV1RUND  RUND  RUND  RUND  RUNT 8'RUNT 8RUNT .RUNT Rate xRunN""qS/N%dSCAN3SMED SMLtSMPLSPACMSPAC|SPACMSVER4.0SVER SVER,,Satd@^Scal@Scan3TUBEF6Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN ;APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSBufT&LCTIDܛCTNMܡCTOwܹCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA&LDATA&L*DATA&LvDATA&LDATA #GDDATA #G- DATA #GtDATA #GDCHTDSamDySN ¥Dye#DyeN°DyeNµDyeNºDyeN¿DyeWDyeW8DyeWSDyeWgEPVt!4EVNT :EVNT FEVNTREVNTeFTab%FVoc%FWO_GATCFeat..%GTyp!!„HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISlOfSc@P1AM P1RL P1WDP2AMOP2BA%P2RLyPBASPBASPCON PCON !PDMF$PDMFPLOCKPLOCuPSZE`PTYPPXLBRGNmܓRMXV1RMdN }RMdV1RMdXԆRPrNaRPrV1RUND  RUND  RUND  RUND  RUNT 8'RUNT 8RUNT .RUNT Rate xRunN""qS/N%dSCAN3SMED SMLtSMPLSPACMSPAC|SPACMSVER4.0SVER SVER,,Satd@^Scal@Scan3TUBEF6Tmpr<UserphARphCHphDYbigphQLcphTRphTR]VX(p:-UZ~en?R/FIOb]_C} IBEBW8w|J"Wl&8{[ Mb.$ru$IC6~bTUV5PZGf$ZP}Gs+OStx0Rx;i,sWYT8FBj'~;Hk@*w&RAXWl>jA:>y7$?js3W4KuYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEefwXAr:3%6AB$AvG9 b?$o0)]j,XXL(y?<&jb!a72BZ|7.N[2MM@Z#bv]ML.Me+bS_?Z p~!*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/VPz-!;9xxsWD[wQi^*D\`??}@8jaxF![pBTd.u?zdq3+f7k7:J&q~}JIXEG%w8.2_/ KRZHlEDx8_1tm9K2="&W,kG<7s[2x*7k'}&8)TK7kx`rxP!t p7Zf_@72tX>VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8K%}jxz)>`VWYhY8|kRYN4+HmL`Kr0k@U-b2k<~jf~Q`xNaKjZD(Z=c':Z^mDt4)TuTF}wKy3of)`|8&kS2BjrP}6YH4HUXYnuPQh^fP%4X+*flT)FTX>EbrB%P^gWT|0\d&nPl'Z9dp"iK]|UKo9UC|T[[66`aD/`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0