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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRCWWWOOOooo''&111;;;EEDNNMWVV_``hhhqppxxx;;;<;;;;;;;<<;;;<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e7e5fef70c74424e9f6ae9a6b525c0dbSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-17_17-10_17603130Seq18139138039_K1336_trnhfjmadera96-Well"####""#"#""###""#"##"####"#####""#"#"#"#####"#####"#########"#############"######"#######"##############"##########################""#""##"#######"###################################"#"###"#""##"#####"##"########"##"####"#################""######################"############"########""#"####"#"#"####"#######################################"#####################"###########"#"######"##"##########"#####"#######""#######################""###########"#####"######"#########################"##"##############################################"#########"###########################"##########""##########"#############################"#########"##"#"###"###"##"######"##"###########################"#"####"###"###"###############################""#"####"######"############"########################"###"###########################################"######"###################"#########################""###############################"##################"#"###################"###########"####"#########################################"####################"######"#"###K1336_E5_Verbenaceae_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zLLMMMNPT[eq~!'0=M`t%Ny/Gdc<^6rbUKFDBBBDHQ]iruslcZTNG<,vkb[SKD?<;:852125:?BCA>93-(%$%),.,& 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")-.+& !(0575/' #'($ !(,+%  !#"       #"       "%&&&$" !%*/4652/,,-/134568:<=<:630-+)'%"                               !!!&*/35541.-,-.0122221100//.-,+*(''''(((&#  #%%!                                  ! !%-8DMQOH@::AM[hrz !! !#'.5;=9/#   saQD:3.(!   &5EWj~7JVZYSK@3$veUI@==AEGE>7118EWkJO~ ^Ml " 6 EF?rA C O#&YA2IX`b^UE.B]3 /Sv(i c+[{qg_YVVWWVSNIECB@=82,'%$%&'%#"!$*4ARey ':=/rf_^dvA("YydP@416E\z0EOOG;.&%,9GOL;iYQNS^sTyqsz[uXJEC@>@Mi>ewt\5vV@4.-,,+))*+,+(!         !  )LurL,  #Cld<!  "Ev0aS6r81 _ rj7# N q!l"5"#"""!"W i u " aupO4}{yyxxwusqpoonmje`YSMIGGIJIFA91,*+.11-& )FnxXA1&  '7@?4"    'Ksc@"3GX_\P>-%*<YwjK.   "$*5F_|/c@J rO2d[  6 J K 8 |J5nyU&Q{k_TI?61/012/)#  """! ##  ! "(2?M\fkg]N>/%          %)($ #*-)           "-<M[dhjlt/Icz{fTA,yh[RMJF>4)                               !!                                                             "" #$##""""! $''%!                                                              {@AAQA,KB.bcpKB_3730_POP7_BDTv3.mobtermAGTCATCTGCGTATACTGTCTGCTGGATGATGAGATGACAAATCAACTAGCTTGCAATTGCAACTGGATAGTTCTTGATGCACAGCGTATCAGCCCGAAGTGGATACAAGACTAGCAGTCATCTGCGTATACTGTCTGCTGGATGATGAGATGACAAATCAACTAGCTTGCAATTGCAACTGGATAGTTCTTGATGCACAGCGTATCAGCCCGAAGTGGATACAAGACTAGC(;Pb =No-Cbz-@Oax$=_w4O[f~%7OZe!2Ts$5I]i   0 @ U k t  (;Pb =No-Cbz-@Oax$=_w4O[f~%7OZe!2Ts$5I]i   0 @ U k t                      I,?b^CQ^@320H/7-_T\(`ADV"X ]Z4J>6"F\)T11RRJ ?U.%U\ L$H2C;P$A )6,NHEL4@2NN*NJN-$;!;6Q.%c F~Y!o(`CvrW=w@E g,>"+TP4(j.Xz)j@GbhL 8LA>eF{E& $xJ ( (* (Q }|gh0@00+g Sc ~PLPp8pp y @J :v|@)@ R J J J J J J J J J J J J J J J J<dp,8,,,do8,8dSd,d,p8}U5 ^&n30,ml85LZR =53) zw`(%<jR:'~ & $NnfWEU9%X4.+5\~ ] & & GCGGCCTGAGACCGCAGTAAACACATTATTAAGAGGCTAGGGGTCCACGAAGGAACTGAAACGGGACCCGAGGGCCAGAAACGCCACCTCAGTAAGAGGAGAATCCACGAGACCCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-17 18:41:02 -05:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtEASPtEB1PtDB1PtDBCTS BufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GdDATA #Gd,FDATA #GdsDATA #GdDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~ ILIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISzP1AMt5P1RLtMP1WDtP2AMt P2BAtt P2RLt PBAStt!PBASttPCONttPCONttePDMFPDMFPLOCt}PLOCtPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT (RUNT  *RUNT 'Rate RunN""S/N%rSCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER  SVER,,Scal@Scan3TUBEE5Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtEASPtEB1PtDB1PtDBCTS BufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA #GdDATA #Gd,FDATA #GdsDATA #GdDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab,FVocFWO_GATCFeat..GTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE'LAST~~ ILIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISzP1AMt5P1RLtMP1WDtP2AMt P2BAtt P2RLt PBAStt!PBASttPCONttPCONttePDMFPDMFPLOCt}PLOCtPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT (RUNT  *RUNT 'Rate RunN""S/N%rSCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER  SVER,,Scal@Scan3TUBEE5Tmpr<UserphARphCHphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23isFbzt\w+6e=ek=c$EpJVB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZ`#6E12 d+[Y5xo|[EG\}d hfz6i8Z26bb+i/_wg=A#Q9nPk@_[O~}<"&eG1h3/[ze~!Tq*nEg8Z~d_1{63_iGbN+7F= E"ae-F*r`s#T)3KO]h^* `v?{}I8w$YWmOyl}$rv/qwnF){xD\4AFUTT3T&^%FyPC,_,qz7A*k|_Lhv\Z& IV4pSqS\c$MiD]JvLY7n nk0WC.%obB`!3<-,RIlVy8J9UTBe$-s:goT|0\d&POhAFDHYWm;2FVDp,CD4;,x8yT,I)rw&^LoT.i792C|5( J!Eb4;,}&v-T)D@4ir$GG\}d GoUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4