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$ "     # %!(*%6='- )%%*%2D6-/& , $   +,7  #7      ('$+9,%"' 8!!2$#      <2#               !6."'(-*+0!          $'                   )** "        ",,III^^^{{{&&&//9DDDNNXaakvv      ***33>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRVVVMMMmml      ***444===FFFOOOXX[aahihpppxxx~~~ !!!"""###$$$$%%%&&&&'''(((())))****++++,,,,----....////00000111122222333344444555556666677777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d4bdd33bbb8944848dba8c62b79ab97aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_08-48_17333130Seq18109137974_K1322_trnhfjmadera96-WellK1322_H4_Poaceae_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z %7Sw "Dl'7BL[owI" q:paUMJJIE>4,(*1=KYhuwld_^]YPB1  |sfXLFEHMPRQOMKJIHGHKORTSNG?83/.-+("  ! 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"#" ##!"$$"                                                    ##!                    a @AHARAKB.bcpKB_3730_POP7_BDTv3.mobtermGGGGGGGGGCCCCCAGAGAGGATCGTCGACTTGGCTCATCCGCCCCTTATCCAGCTAAAGGATTTTCTCTTTTTTCCACTCATCATTATTTATTTATTCTGACCTCCATACCTCGATCGAGATAGTGGACATAGGATGCCACTTTTTAAAATGAAAAAAAGGAGTAATCAGCTGTGACACGAAAAAAAACGAATCCTTTTGTAGCTCGCTTCCTTATTGACAAAAATCGAAAAGGTCAATATGAAGGAGGAGAAAGAAACAATAGTAACGTGGTCCCGGGCATCTAGCATTCTACCCGCAATGGTTGGCCATACAATCGCGATTCATAATGGAAAGGAACATATACCTATTTACATAACAAATCCTATGGTAGGTCGCAAATTGGGGGAATTTGTGCCTACTCGGCATTTCACGAGTTATGAAAGTGCAAGAAAGGATACTAAAATCTCGTCGTTAACTGAATTCAGAATAGAAAGATTCAGAAATAAATAACGAAATACCCAATATGTTGGTGGGGAAAGGGGGGGGGCCCCCAGAGAGGATCGTCGACTTGGCTCATCCGCCCCTTATCCAGCTAAAGGATTTTCTCTTTTTTCCACTCATCATTATTTATTTATTCTGACCTCCATACCTCGATCGAGATAGTGGACATAGGATGCCACTTTTTAAAATGAAAAAAAGGAGTAATCAGCTGTGACACGAAAAAAAACGAATCCTTTTGTAGCTCGCTTCCTTATTGACAAAAATCGAAAAGGTCAATATGAAGGAGGAGAAAGAAACAATAGTAACGTGGTCCCGGGCATCTAGCATTCTACCCGCAATGGTTGGCCATACAATCGCGATTCATAATGGAAAGGAACATATACCTATTTACATAACAAATCCTATGGTAGGTCGCAAATTGGGGGAATTTGTGCCTACTCGGCATTTCACGAGTTATGAAAGTGCAAGAAAGGATACTAAAATCTCGTCGTTAACTGAATTCAGAATAGAAAGATTCAGAAATAAATAACGAAATACCCAATATGTTGGTGGGGAAA$*4ET_gu{ %3>FRZi|%2@IP`mv'3>LWer,7BPjs*4ALWdq})8ALXco}'2>JVdoz-9GSalz    * 5 A M Z f s   ! 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"   !  ++# PMH_<<#G[L2c:b"<[*4KS<LF-J;(?#@ 0$JDB^&((3WDISS6I`+,840[`[1:Z%[PC0@!.A "-7K92.C4;> %>#>Td7HJbS<4- #PW ,2 >`_ "@8Ha_O.GH6," Xa4R! !"?`IGSR3WX ]Q'?)]L%DM0'WU `a.BT^1]9O?6=<XD_H A'"7N_14DG5U!+)I&^H/7// .K@I'S[:V<C05:_DL# **L5GcZ*UF _aJ=S:,HPGU%7E-@cR%?K>%?#3] I DZ/DK!Q)8+T0QO//2WK79WGIEBT&)\b++91@]SCD"SRW$!2_K2#+2=31YY2AMZI2;'HJ5-$*Q:P/22OKR G2\V*:1`T)*:BJa20&#_2bK(LS0,BETAT-ICA>A@2(P22$$aa!E`S'9-:1YUM&,:5 JR"d#n" :TnDH0b:tCiqF}H/^u1\)gL']kh- |@rdd Ira_7 /.XI"+0m2vjx:$ >tN0E% vB-r XP,Flg1&Ubu~F4 Y"w}8{{xdW@?pUu>aEZc3=?"x,xlc`J lt5,)$Ip5vH =9ZTh=:g#GiKUkp__&} _)bfd/)O*)[Bai/TG2|Mg0 Zv+!@PMq]#91;x r*ad B4zP|w G b E,)s.X#<8RmuX\==^]_.J>FD#< vO>X2O='Q->mY`BES T]D>G);  ~  \'=LYW 2#!3:  Z 2    (+ )&,A5  = )VQ>+,j@0CF  .#'*  (x3   r .   &   GS 6      7 Z  2    :c@rp82Tapp|>(p 7:'Lp(ZpF4O2,y8;8u-8p.t" (G?A,5jP[><\TjQE2]we3eq+Z7s#S#'$3,D8RY) !q9D=B#LnVhTIS^E<1V3p thUEDj6Kw9A^(;[nr=,%p>Ial8)p F _<35 p,d8SQ2@D699X#P^  l |HD  fcP4v7CH"oYmYW)}G1<1M<- > kb9^+17\n$_?S;=0HF&G_ *)9Dd2tufG8-  #07+4'&-R#3Gb04F)'w>H.Mx#;%2+qK375$SPJB<<H,&V6 LZ3V=# D-4)+EDmJ(;?*He7w9L9+#x}+9L>TM\iU$LHc&2%2=gJdi4(n.=FFYv7FJGgys5L2K{Qbz|]^9JDftVwrBf~bGavW%LlwE?>C}h@:}:LSz<YH3]mxdIvsNfdJ`x_2uO+xkP ^UUf rc?xE|Iw{S#xceVtqgLH]x7DQl,AAAAACCCCGGGGTCTGTGACCCGAAATCAGGTCTAATAAGCGAATAATATACAGATGGAATAACCTCTCCGGGCTACACACCACAACCCCCCCATAAAAAGGGCTACACATCACGCACGATAGAGCCCACCCCCTAAAAGAGGCCACCTGAAGGCCCCTCAGAGTTGGCTTCTGACACAAGGTCTCGCAAGGATGGGAATGGATCTCTCCATCCCAAAGAGGTCGGGAGCGGTTATCTGCCCTTAAGACCATTTTGGGGGGGGAGGGACGCACTGGAAAATATAGATTAATCTTATAAGGGACAACCGGGCTTCTATAAACCAGCATGACCGGGACGTACCCCCGGACAGATTCAGCACGGAAACGGTTAGAACGTGGCCAGATTAAGGAACTCAGTGTAGTCAGGCCATCACTTGAGATTTTAGCAGTCTTGAAGAGAACCGCATGTTATTGATTTGTTTGGTGAGTCTACGGTTAGGCTGCTGTCGCCCACCCCCGTTTTTGGGCGGATGTTAATTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 10:35:47 -05:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSfBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA *!TBDATA *!TB@DATA *!TB2DATA *!TBtDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~dLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS=P1AMRAP1RLN1P1WDVQP2AM^qP2BAbP2RLZaPBAS?PBAS=PCONL)PCONJ!PDMFPDMF=PLOCFPLOCBPSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%=SCAN3SMED SMLtSMPLwSPACA`/)SPAC=SPACA`/)SVER4.0SVER fSVER,,Scal@Scan3TUBEH4Tmpr<UserphARphCH=phDYbigphQLcphTRphTR6큅This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSfBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA *!TBDATA *!TB@DATA *!TB2DATA *!TBtDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTabtmFVoct\FWO_GATCFeat//t-GTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~dLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS=P1AMRAP1RLN1P1WDVQP2AM^qP2BAbP2RLZaPBAS?PBAS=PCONL)PCONJ!PDMFPDMF=PLOCFPLOCBPSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%=SCAN3SMED SMLtSMPLwSPACA`/)SPAC=SPACA`/)SVER4.0SVER fSVER,,Scal@Scan3TUBEH4Tmpr<UserphARphCH=phDYbigphQLcphTRphTRz^4?FVwoyljyK{t61h67A"yV+XZ{8+;N}?"\=QtAN-GQ6)l4;qr-.^_Nxq^*>H~?p96|3Q$kv@1=,Q9\qwHN0&)Bk+7{lZpKrS|YYw>xPqL6`nY?}xj/99xvT+,%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KKVd~65nbJEZG59F~q,/5`w=yBimJEIoM/V,<|ag&<{[;NK-fU'C,_,qz7h}l6t8OGz^n7aC.?bFW?E)X~6"den?,}!KPk;+s4U5hf6]X?K90lV5FaGwpBz.\_'glxU)Bk+7{lZp4[8e7?GOVcRohj4TIx*&`f5U_9%jE6; 0LBqmI,ComMOx+6T,I)h'_17a`<{!s73tt:8JX|K2MWhceS-o"KBv'o>s1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-:d*V2J<\6Y zoH6iAC?-4$&)-%TwK[*oCfty5lYMI){mnjH[$.}RZ#3|>oW~!zFHyErN3W4KD@|Q$OEH< z!$w1QjXaM~PG"BXP2gXBrRLohi\`hlZZdBc'}_~:H^)!,8Kp/(ZA$den?a+b8E|W,K4vqb>H\R~.i58f{sGi8AS_9\qwH=qd)B*vH%lFUT;fQGE-`x&iG_C26R&^8o5`h]RIUwch[}k@_a'>yj6m}Pz%W%43q*)_RhGlB^PY6C}B-m2yy>T\3>>B]'ngg61C`wWsV&1