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!!!"""###$$$$%%%&&&&'''(((())))****++++,,,,----....////00000111122222333344444555556666677777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 57d0d722890f45199d1712b8d1e5650fSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_08-48_17333130Seq18109137974_K1322_trnhfjmadera96-WellK1322_H2_Melastomataceae_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zEFHKQYajr|-;EMVao  wmdXK;* ~umga\VOHB=96443444420.--/36998530//..,*'$"     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Ez  v>!)V1WV/](    0`n8,bVub#o..j).M   "L~J!  )Mwd:  7`[3   $QJ$"E99G 7^pH%   3X}a<    @us? 3axF"+XM#    (Q^4     )S^.C'!m7"5j!*X)  ;`|Z77`zN'   #K|i; !@r`2  $AbpO.  (Mw}T/  !@dhC#  ?fsM0&5YyJ&  ,OtnK+    .S{g@% 3ZtG$    #Hu^6#+MxH"   0SvkH(  %Ba{~fI.   $$       #%),048<>ABDFIKNPRSTVY]aeilopqrstvwy{}~|zwutssrokfa]ZYXWVTQNLIFC@=;:863/*'$#""!    !#&(*-15:?EKOSUWX[_ekrx}zvronlkkklnrvy{||{zyvrkbWMD=951,%  &.7AKT\chknprrqmhaYPHA:4.)%!"'-39>DKT^jwH~ ^t"p9v>*nl%J>-Y .KbsoQ)H U"{sonnpqrt{T;P2fS cJGoea_][YXWWVVUSQNJGC@=;852/+)()-2;H[u,aJvw+~5:{#nO;1/5AUsaI~eJ;9DUdhZ6elSJPd=9zZD:;GZpb+B{V@51/.,'!8ciReyK0"!%'))'#.U= aUr= *O.6`c>=T*  'TM#%Y^0  A@CK!    U~-M;4s4 %[wED  "%)/4:AIS_m{=o!eYgVQI '  M p | ` 2 B`aP?BcHt@vj_VOHC>:76566530-+++++*('&&'(('&$$$%'())('&'(+-/.,)&$$%(+-/001110.,++.158;<>?>;4+%)@prnoC008ADB<4.*(('%#!#&&"1e7fwg7c0 !'&    "SCH+l6 !   !'.5=DLVan~3Mi"3BRcunZG6$lT:-Q_R*tF) %(&#     <v-@2N&  I V+     (W!U+    %RP&   ,US-   @m_7  (QXMc@G+{>B*{wsqpnkf_XSONMKE;."(Cmk?!   .ZoB    /[c9%,LypF%   +SrF#  :km@    7fW/   3^e:   &N~oB    Cs_5   Bph>$#9`yP-  8Z|`>!    0Zl@    &Ho\:   -RzY2   9`wQ.    8Yy~`A&   2Qow[=" )HhgI-    /Nmu[?(   .PwkI-  $+.,'  0ajD13CZluqbM5! &2=DGC9,        M7@AeAFCArKB.bcpKB_3730_POP7_BDTv3.mobtermGGGGTGCGCGACGCGTATCGACGTCTTGGCTCATCCGCCCTACTTCTCGTAGTAATAGTATACTACTTCATATATTTTCATTTTTTTTTTTTGAATCTTTCTATTTATTTTATTAAATACTTTAATAGACTTTAAATCAACAATGAAAGTAAGGGAGCAATAATAAAAGTCTATTGAGAAAATCAATAAAATTATATTGCTCCCTTACTTTCAAACCAAAAACTCGTCTAAATTCAGACTAATAAAAAAGTCTTATCCATTTGTAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGAAAATTATGAGCATTACGTTCATGCATAACCAAAGGGGTGCGCGACGCGTATCGACGTCTTGGCTCATCCGCCCTACTTCTCGTAGTAATAGTATACTACTTCATATATTTTCATTTTTTTTTTTTGAATCTTTCTATTTATTTTATTAAATACTTTAATAGACTTTAAATCAACAATGAAAGTAAGGGAGCAATAATAAAAGTCTATTGAGAAAATCAATAAAATTATATTGCTCCCTTACTTTCAAACCAAAAACTCGTCTAAATTCAGACTAATAAAAAAGTCTTATCCATTTGTAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGAAAATTATGAGCATTACGTTCATGCATAACCAAA&1M[s +6BLVbu %5@PYanz&/:CQ[ft*7CNZeq|&2<FR^jt(5?KWcny !,7BO[hr| $/;GS^ju   " , 9 E P \ g r }  ! , 7 B L W b n z    ' 1 = G R ] g s |  ! , 5 A M X b l w    % 0 ; F Q \ g s ~ '3>IS^ht&1M[s +6BLVbu %5@PYanz&/:CQ[ft*7CNZeq|&2<FR^jt(5?KWcny !,7BO[hr| $/;GS^ju   " , 9 E P \ g r }  ! , 7 B L W b n z    ' 1 = G R ] g s |  ! , 5 A M X b l w    % 0 ; F Q \ g s ~ '3>IS^ht      "  >0&*1 /3>>38'/>>>>, 8>>>;>>>+'>>   ;86>;>>>;>;>6;>>6>>>>>>61*2  (.--&8188>>>>>>>>>>>>>>>6;>;;;>>>>>>;;;;8;;;>;8>;>>;>;>;>>>>>>>;>;>>;;""'2;.8;;>>>>>;>;;>;;>>>>>>>;;8>;;>>>>>>>>>>>>>>>>>>>;>>>;;;>;>>>>;>1;;;;;>>;;>>>>>>;>>;>;>>>>>/>>;8>888/>>,,>83%2        "  >0&*1 /3>>38'/>>>>, 8>>>;>>>+'>>   ;86>;>>>;>;>6;>>6>>>>>>61*2  (.--&8188>>>>>>>>>>>>>>>6;>;;;>>>>>>;;;;8;;;>;8>;>>;>;>;>>>>>>>;>;>>;;""'2;.8;;>>>>>;>;;>;;>>>>>>>;;8>;;>>>>>>>>>>>>>>>>>>>;>>>;;;>;>>>>;>1;;;;;>>;;>>>>>>;>>;>;>>>>>/>>;8>888/>>,,>83%2  AG>P1M&&c+7RQ8 S!0P.!Q0#7; OK 2>]M'Y20R!K*F!bO8\%=S<d_Fa>#U_07IDH_X $9:85*D7"2X"GIFX8TK %/<^E#8BV'_%% +T#%CV:+2_#.^J@/2%0WDP5I`NX\32.M(:_17;A\\MG@^*]['VW EFZ.'LSWO-UZ%_^CU F55 WX2W<AL :, C"6b@!a AV)26.MP&)P@ ?H_E.?8(4`QEN% ;!<\[7*b/=* Z 'tLPJz.C.NsQ 5|Gu3GKPK" ):57> Y L7Y^n*Q/>*A-FTN@u(!lHbh.{SI)i_jNfD2*%MzLuP6>;Do=  H*O y.AcdHGvPeO f[2[ry K   GOX V+a2ErkxWcQkUbZgU:V6J99;/!/2> -9#A6 y~X:F      +&      ; wpD pgpYpp z~ppA#eNA8DuQO.[`wbo _9L $~N/ 8a@F#h8 dvwX4bpWdip[t9K^V+klu8-8s/:[dyr 6!UfKTI&}p-yX)*sjCQo^x.U  b [dQh>C%LE>?7&8 F@0 %:#,@x8`S0,S'+%=&Q << t`8 2{<@)(6>O*57!*_3*^BD)90&27YU8U=~[2 \(WL' 6.Y``$M+\]kMtac}j:$%EB5 :i33(IDC&3-\=u%NM+*81 !F1\I,J5>L*$=xR(WEP1-9#8Q@RrD7N5L2R<"D;O+dQM/AZ<H7;sx'=7%WkFAQwT l5;:qAAAAGCGCTCTGTGAGCCGAGATCACGTCGAACCTGCGAAACACCTCTCAGAATTATAATACAATACCACATATAGGCACACCCCCCCAAAAAGGGGCCCTATAAATAAGATAAGGTATACCCCCCCCCTAAAGGGGACCACTGTGGGTGTGACAGCGCTATTATTGGAGTCTAGAGAGGTTATCTATCTTAATATAGTGCTTTCCCAAAAACGGAACTTTCACTCGTCTTTACTCAGACTTATTGGTGTCTCATATTCACGTGGATGTAGCACCTCCACAGCCTCCATGTCTAGAAGGGTAATATGAGCAATACCCACAAAGGCTAACGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 10:35:40 -05:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS!BufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA #BFDATA #BF3:DATA #BFyDATA #BFBDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISP1AMKP1RLKYP1WDKP2AMKP2BAKKGP2RLKPBASKKLPBASKKPCONKKPCONKKPDMFPDMFPLOCK -PLOCK PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%SCAN3SMED SMLtSMPLwSPACMSPACSPACMSVER4.0SVER !SVER,,Scal@Scan3TUBEH2Tmpr<UserphARphCHphDYbigphQLcphTRphTR4FThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS!BufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA #BFDATA #BF3:DATA #BFyDATA #BFBDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTab/vFVoc/eFWO_GATCFeat///6GTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISP1AMKP1RLKYP1WDKP2AMKP2BAKKGP2RLKPBASKKLPBASKKPCONKKPCONKKPDMFPDMFPLOCK -PLOCK PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%SCAN3SMED SMLtSMPLwSPACMSPACSPACMSVER4.0SVER !SVER,,Scal@Scan3TUBEH2Tmpr<UserphARphCHphDYbigphQLcphTRphTR[4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95E3&iL!=3]zUTs5+b9>$:EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHxh$v1)ciA]!lzc:B?6pyNmdD9ohf|39bT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m*0_fs$X=<;#(eaK|`!t/[z~+7D>0Mt9+'v`R(fukowMq1R7R>(hZ$~I!|spN$t.Dd^4G^({C!RJ<6Q^Zew.k.d}uNf1&vM\#YTEl?&azzHFj4ua@xS3"|oIx5n|8EDG7v-t1)o0/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@