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"                ) $.'$+1 -   "  %!    $                                          ",,III^^^{{{&&&//9DDDNNXaakvv      ***33>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRVVVMMMmml      ***444===FFFOOOXX[aahihpppxxx~~~ !!!"""###$$$$%%%&&&&'''(((())))****++++,,,,----....////00000111122222333344444555556666677777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 72316897a7d542c6a2dd8a34a45216feSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_08-48_17333130Seq18109137974_K1322_trnhfjmadera96-WellK1322_E3_Peperomia_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zBDHMT\addfipy{wsponopqrrrqomlllmnoonljgda_^]\ZXTOIC>:7530-)$!     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"'+-,*('(+18BMZiy~wnbTF9-$          !!                       ##!                         $*05:=>>>=<;:9889;<>=<9631/-+)'%#!                                                   Q\@~AQAFcAjKB.bcpKB_3730_POP7_BDTv3.mobtermAGGAGACTTGTTGTGATCGACATAATGGCTCAACGCCCTTACAAACATAGTACTCTTTAGTAATTTAAATTTATATTTTTTAGATTAGAAATATGTTGCAGGAGTGATTATTATCTTTCTTCATAAGATGTAAATTATAAAAAAGGGAATGAAAACAGCAAACTGGAATGCACTACATCCCGAATGCTGTTTCTTCCCCCGATGATAGATTATCACTGAGAAACATTAAAACAGGTATAGGTAACATGGATCGAAACCATACCCCACTTTGTCTATGGCCCTTCCTCTTTTTCCCAGTCAATCGGGAAGACGAGAGCCGTACGTTTATGCATAACCCATTCGACCATCTACAGCACATAAGGCATGGGGGGGCCTCAAATTGGTAGACTTCCATGCCCTACCCCAACCGAGGAGACTTGTTGTGATCGACATAATGGCTCAACGCCCTTACAAACATAGTACTCTTTAGTAATTTAAATTTATATTTTTTAGATTAGAAATATGTTGCAGGAGTGATTATTATCTTTCTTCATAAGATGTAAATTATAAAAAAGGGAATGAAAACAGCAAACTGGAATGCACTACATCCCGAATGCTGTTTCTTCCCCCGATGATAGATTATCACTGAGAAACATTAAAACAGGTATAGGTAACATGGATCGAAACCATACCCCACTTTGTCTATGGCCCTTCCTCTTTTTCCCAGTCAATCGGGAAGACGAGAGCCGTACGTTTATGCATAACCCATTCGACCATCTACAGCACATAAGGCATGGGGGGGCCTCAAATTGGTAGACTTCCATGCCCTACCCCAACCG&BP`s -7BNVbny+=FWfu)3@KXft| +6DP]hs#/:FS^jv$.;GU_ly %.:FS^it&3@LVeq~    ' 6 A L X j v   * 5 @ N Z c n z    $ 2 @ I U a m x    * 5 C P ^ i t     ' 2 @ L W c o {  !-9ET`kx*4>KVcq|(3@JV`iy *7ALZhu )5>LWdq| '3>FT_is~&BP`s -7BNVbny+=FWfu)3@KXft| +6DP]hs#/:FS^jv$.;GU_ly %.:FS^it&3@LVeq~    ' 6 A L X j v   * 5 @ N Z c n z    $ 2 @ I U a m x    * 5 C P ^ i t     ' 2 @ L W c o {  !-9ET`kx*4>KVcq|(3@JV`iy *7ALZhu )5>LWdq| '3>FT_is~                                  "+     "         ' " "        )    1 $           "                                    "+     "         ' " "        )    1 $           "  U_X4GI%:*U7VD"" CH9&S E( EVK+_7F5;3KAD()5K_BbHC "-!SL:'[:>LWA2IY ^^E F,- bGEY'MR>L? 'P#=$C#44 "U8LJ71c+?-JH9H[aa<HTFM/7--TKc<(=IY?% V<25E P(@R )$J]1P<(<CZ2 EPTEEEEEE:L(2 [&EO KE&.6E @=M895%2?X #)2;DMM2IMU9_L#WZ1_6V O;\Z@VVV323V#V]FV?W2<2LH:L12V5V]G2V)13,_$213Vb"2YPVZ;2_)O9\,nY Y s~?n2 +3"mtq- *x.}5^QZ[`  X y<- j@i0)DiRA?)$` ZhP7$ y~<#`V-[R[:k41f[ ? V7{<BJM77?9Qq&5m+nxfhB v C(#tU#urHy.[~; CDmiM?JI}a@Jkq2]smEr|cp87;&S V( '=Y  sF* (` 3kSDe}V 7rGoWO;)L7xFUesus^eymKr;u!IdC)0. v=-- (.NLp%aejwQ"* D/& M./ 5S 1[<33=A36*3-R33,%MJD 9%#. 9 sx8 1 iBSo8.[pppypip*D7C}IA9<pQGpxOdY/Z]<JB3'Ek:)!9Y*@O$1YZ+)g4^8Q{K2Ech& i,j ,8KDMx6;m[MK p8o,; 'J}!WI4r'{rdj*Zhk8,?ed-'&d;/syF#W\W 9;6^tY;?Bt2(*ls{Qd>$|!W@"vA %.W& 8j?7uk:"Hw*s\idAh7G]].+sW!,6_0@)!t2  ((=x.&L[ZEunQ *dVB;BG;l *4kdh UQ4bfw-jZ9yg'"DF|4uNwOhTJ-fI!)Se` P(s$Wl#n9] B'Ai4{>VIcMr^:?3pf!$5.$*0[9iGACGCCGCCCCGTCACCGAGGTGCCCTCGAATCGCGAAAACGTGCTTGTTGCTAAGGCCTACCAAACGCAGCTGCCCCCCCTAGAGGACCTATAAACCTCCAGCGAGAACAATCTCCCGCCTTAGGAGAAAGTCCCCATTGTTCATCTTGCTGCTTGAAGTTGGTCTGATACAGCTTCAGTTTTGCTCCCCCAACTTTTTAGGAGAGCTACTAACGGAGATCCTGGAGCCTTTATACATAAGGGAGGCCGGACGTTGACATTATACACAGAGTACGTCAATCATACTAGCAATAACACAGGGTATACCATTCTACATTTAGGTGGATGCACATGGAATGCTACTTTGTCCTCTGCGCGTTATGGCACAAATATAATTTTGACCGGACGACTTGATGGTACATAACGATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 10:35:48 -05:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSlBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA +WDATA +WDDATA +WDATA +WDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~kLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISLP1AM2\P1RL2YP1WD2_P2AM2fSP2BAiP2RL2c!PBASN\PBASLPCONWPCONVYPDMFPDMFLPLOC2S'PLOC2OPSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%LSCAN3SMED SMLtSMPLwSPACMSPACLSPACMSVER4.0SVER lSVER,,Scal@Scan3TUBEE3Tmpr<UserphARphCHLphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSlBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA +WDATA +WDDATA +WDATA +WDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTab{FVoczFWO_GATCFeat..zGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~kLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISLP1AM2\P1RL2YP1WD2_P2AM2fSP2BAiP2RL2c!PBASN\PBASLPCONWPCONVYPDMFPDMFLPLOC2S'PLOC2OPSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%LSCAN3SMED SMLtSMPLwSPACMSPACLSPACMSVER4.0SVER lSVER,,Scal@Scan3TUBEE3Tmpr<UserphARphCHLphDYbigphQLcphTRphTR_R|!{VmgMm[*&`cz"B``,_bX@sWFa}TYKt9>,3HB! 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