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!!!"""###$$$$%%%&&&&'''(((())))****++++,,,,----....////00000111122222333344444555556666677777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b10f963a125b46a9b407ea9712e84d55Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_08-48_17333130Seq18109137974_K1322_trnhfjmadera96-Well!K1322_E10_Salix_microphylla_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z+8DP^nyhT<# |tj`VOIFCA><976555568:<=??>;84/,*)))(&#    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Y,@ FgY,9^iVm|']DrY6qd6n@rBg~^@Jl? ; ^QjB#UXw0- vLWdj{B;@3+c*Rt)j) ra|Sjr|~A}]%9S@v=+Q\6~B~@od+Cg8c'od7x l6v] JSk.'Q]sF Heb nsg#7M[7-h9nck^]yWWW]Tf=Iih<UXQ+12 aa&'A&46=*u8|K  '?6  2  0 $              ,T8M, CqM(pW`8&TV#)^pGd.],`5aGAdAPXZ$}vk8OlV]YG+@ZR#&,DN*pkZf ^4oE6QnW]L8$B[jtx(/""[7 HyW-[QW]~ Es?:,6zClD '#G5DEyj1% # 3      &"" % hzSXs<OYj[- 5B/*%*' K=Q0(+1=6F$<'C"1&(4(CB#?=7?!18-$*E -*+:#>d(2 6:74VVJFFPAAACCGCTCCGTGAGCCGAGATAAGGTCTAACCTGCGATATTACACTATAAATACGGAATCACGATACTCGTAACTATCACGCCCTCCAAGGGGGGCGACATGACTCATGTGAGGGAGGTATCCCCCCCCTGGGGGGGGGCGGGACGAGGGGCGGGGTTCATAAATAGAGGACGAGGTAACCGAGGAAGGTAAGAACGAGCCTCCCCCAAAAAGACACTAGGACGCGGGTCTAATATTTGAGTAGATGAAGCTCCTACAGCAGCTAGCTCTAGAGATAGGTAATGAGCATAACGTGCATGAGGCGCAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 10:36:14 -05:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS]BufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA +V DATA +V BDATA +V DATA +V DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~\&LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISDP1AM6lQ@P1RL6lNP1WD6lSP2AM6lXP2BA66ZP2RL6lVPBAS66FZPBAS66E$PCON66MPCON66LhPDMFPDMFEPLOC6lIPLOC6lGPSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%DSCAN3SMED SMLtSMPL""wSPACMSPACESPACMSVER4.0SVER ]SVER,,Scal@Scan3TUBEE10Tmpr<UserphARphCHEphDYbigphQLcphTRphTR'1This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS]BufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA +V DATA +V BDATA +V DATA +V DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTabl FVockFWO_GATCFeat//kGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANEHLAST~~\&LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISDP1AM6lQ@P1RL6lNP1WD6lSP2AM6lXP2BA66ZP2RL6lVPBAS66FZPBAS66E$PCON66MPCON66LhPDMFPDMFEPLOC6lIPLOC6lGPSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%DSCAN3SMED SMLtSMPL""wSPACMSPACESPACMSVER4.0SVER ]SVER,,Scal@Scan3TUBEE10Tmpr<UserphARphCHEphDYbigphQLcphTRphTRTrU]wfgM6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! 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