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" ($$! %.%  ('    ## )      "       !       %&                                                                                                       ",,III^^^{{{&&&//9DDDNNXaakvv      ***33>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRVVVMMMmml      ***444===FFFOOOXX[aahihpppxxx~~~ !!!"""###$$$$%%%&&&&'''(((())))****++++,,,,----....////00000111122222333344444555556666677777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9da9ab466228498e8c94966fa9b74926Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_08-48_17333130Seq18109137974_K1322_trnhfjmadera96-WellK1322_C1_Asplenium_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zlnqv} zoheegiihecbcfjmnnkgdaaaaa`^]]^acc`[TLE@>==;82+#     &,4=IWfuwmbUF7* "-:IZm"9OdvlWA)n^SKE>81,(&&%#   ",9I\r;b oFxomu 1TsmM*{`H6(  -Ec @w %2/qf\SH<. ~aF.  #5Ke qR8$ "9Tr&F\e`L,~X8  5\[ai@" '<Wy"@QSF, Y5#2E[spV<'  'Ac wlosK* .P2a{|e:_:    (AauhjzxZ>'    6UwiK2  5MhsV:#   *AXnp\E/ "'))(&%%(-29AL]v #}wmea```^ZVSU\j|w\C.    -AVgqrj[H3!  2MiuX:  %Iw Y1 +Nu{V6 #>[tpYA+  !#$$$#"""" &3EXisum_N<-"    1Mh~{dH.  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SeBH*HxS=k[0{>LncD7 ?1V$tH;%xr+X:AO&5!G3(B("6"/&&VwDp`WFD-*/7/H$E=0(8CX{C:%bB*(#08<#&2G$$.M%!]8EABBk1.TW)ONaRaqWA;-%H,-5:G09@7"=E-DB/3)#:W54@YHL<:H%*EB:20QiF2AbV> 7CP>GBj[d1N134HFMK1&=B,k2U?T4WW@GKrPg87oNpRoc9QZ=Jd>=VcD_6]TdI1d3=/EcswWL;5\]1wyTCX@O4HYgw>I+&6jBqI]YVlm:L5wk^<IheepI30cTp3Y1TaZPPJ'Q% h]nDB2WJP\l]ZuPH;*&2e a1;P+R $ uIAAACCGGAATCTTTGAGCTAGACTGCAGTTTAACCTGCGAATCTGTGGTTAAATAGGCCGAGTTAAGTGTGGCTCACGTGCACCCCCCCCTAAAAGGAGCCGAGGGCTCTAGCACTGGGTGGAGCCCACCCCAGGAGAGGGGCTCGGAGGGAAGAAATACAGGCGGACGGATAGTCATACACCGCTTGGCTAAGTTTTCGAATTCCCTCAAAAAAGCTGGCAGTGGAGAGCGGGGTTGGGACTCCCCGGGAGCTGTAATAGAGAACCCGCTGACCACGACACACGAAGAGATAGAGCTAGCATACCGTTCCTGCCGGGAGGTGGGAAAGTAAAATAAAGGGGGGAAGCACGAAAACACGGGAGCGTAAATGACTCGGCATTGTATCTCCGGGGGAGGATGTGAGATAGCACGGAGGCAGGTGTTGCTGAGCTGGCGGCATGTACTTTTTGACAATTTTACGTTAATTCGCAGGAGACCAGTATAGACCGGAGATATGTGGGGGGCGAACAAACTTACAAACCAATGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 10:35:43 -05:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSV,BufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA (P"DATA (P"<DATA (P"DATA (P"DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~TLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS-@P1AM BP1RL =P1WD F3P2AM NsP2BARP2RL JSPBAS/PBAS-sPCON;PCON9PDMFPDMF-WPLOC 5PLOC 1PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%-8SCAN3SMED SMLtSMPLwSPACAcԆSPAC-PSPACAcԆSVER4.0SVER V!SVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH-nphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSV,BufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA (P"DATA (P"<DATA (P"DATA (P"DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTabdFVocdvFWO_GATCFeat//dGGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~TLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS-@P1AM BP1RL =P1WD F3P2AM NsP2BARP2RL JSPBAS/PBAS-sPCON;PCON9PDMFPDMF-WPLOC 5PLOC 1PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%-8SCAN3SMED SMLtSMPLwSPACAcԆSPAC-PSPACAcԆSVER4.0SVER V!SVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH-nphDYbigphQLcphTRphTR/o'zbMaQd&79?OkZL9,,vk1G&M6] 7UkAxBd?DSGwvlAc3_{#RX#W+BcK;o`H9T+a:WkR,uKsK[NHu` *R@_AiG3mwYgE@f9kf'C#/\+F\%_krjZUTw|bT8Poo=[/19~{yrA]#_WfOMt_X`@kjJ}B~xGH$i*8j|]]u.ibu@Qhiq|fq:#6D:_f7o,f9r;qBAK$M@at0<EkP9bA:>y7j] !3xnv_YDkrD5pYy7,Fb[2yy>T\3>>B]'p8Npd-(bcn23isFbzt\%+6b=}V=p%E+!`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>V3jo>E7-8rj~n.It!TQ'O1^m?e({ln}sFZ5uLJ:.<)%,8EE.Om,P>bH6X128)TK7\4v3s'kY6C}B-mq5FaT2V3pVoqCH-p@v\vhsW__Z';fQGE-`x&iJ-,qzjN"o+Ax8amb,.63<%Ri24WF=-U[D~TWY+N@c'v+gK >o>s1H#"$w8@&!]T68n(0Ju*zv&1M2F{H/`WajZUTw|INi@C,6KP8)s5AVihx_7&OMb=#w&bN"@7hhSc%bVm? 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