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" "                    #                                                  ",,III^^^{{{&&&//9DDDNNXaakvv      ***33>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRVVVMMMmml      ***444===FFFOOOXX[aahihpppxxx~~~ !!!"""###$$$$%%%&&&&'''(((())))****++++,,,,----....////00000111122222333344444555556666677777788888999999999:::::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 49486c001b6e4aee9339d3d557d426a8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_08-48_17333130Seq18109137974_K1322_trnhfjmadera96-Well!K1322_A10_Quercus_candicans_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z%6CMT[bkvhR>0'"  {k^SMJHFC?;62-)%##&+/11.+('&&%#! !""!     #+7H\s.Gb}}dM9&eRD8/(#"&-5=BGMXj@d &<MTO;bB"}ume[RIA:50,(" #%&(,3>L]o%aX/[{uQ"N!oO0 )Lu0]c6o< wdVLC;4.*'%$#! !  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D/+5 %&$#:")*>9!'4-I,;M(8',%Ak1eV",7.M:@)$e(SD7$-<P;,>D'4PE4C7('S.b!*#OH9%>)!7%<. &->6$29/PbBYa#,oCvIK056-F(BO?'G"k,P;'A$N8ELC/-(e]w';%0-LJ7K}DZ Os_JF\.lF,M)3~E"'A@0%=%-@]E1Gf$aV:[&;Lh~3\Ni;4NQ/tsqSz>Ot]28Ssn4c:GK:YO%itpW'RWL7p{`o_w_bk_R|uRijX3<gq$P1B|/P duA85[CF4N7<^AAACCCGGCCCGTCAGCCGAGATCACGTCGAACAAGCGAGATCTTTACACTTGGTATATAGAATAGGGAACACCGGTAACACCCCCCCCAAGGGGGGCCAACGCTGGGAGAGGAACGACTAGACCTCCCCTGAGAGGGGTCACGTGAATGACGCTACTTAGTGGGAGCACGGTCCCCAATAGCCGGGGGGGGGTCCCGCGTCCTCTGCCAGGGGGTCGCGTGTTGGGGGTGGAATGGCTGGGGCGTGCGGGTCTATTGTGGGATTCGACGGCCCATCGAGAGATTTGGGGGGCGGCGGGGGCGATATAAGCCGATCAGTGGGCGAATTTGCGGTGCCTCGTCCGTAGCGGGGGTTGCGAGGTCCGCGGAGCCGCCTGAGGATGTATGTGGGATGTTGCAGGCTGGGCCTGGAGGCATAAGGAGAATGGTCGGGAAGATGCAATCAATAAAAAGAAACCCCTGGCTCGTGTGCGGGTGTATCGCCCTCCCCTACCCCTGGTGGTCGCGAAACGACCCCCACCCCGTCTTGACCAGGGAATATAGCACAATTTGAGGGCGGGCCAATAAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 10:36:19 -05:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS_hBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA (QDATA (Q>eDATA (QDATA (QDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~]LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS3qP1AM9rIP1RL9rElP1WD9rNPP2AM9rW4P2BA99[P2RL9rRPBAS995PBAS993PCON99C3PCON99@PDMFPDMF3PLOC9r<PLOC9r8PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%3iSCAN3SMED SMLtSMPL""wSPACAbSPAC3SPACAbSVER4.0SVER _]SVER,,Scal@Scan3TUBEA10Tmpr<UserphARphCH3phDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS_hBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA (QDATA (Q>eDATA (QDATA (QDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTabmFVocmFWO_GATCFeat//mGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~]LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS3qP1AM9rIP1RL9rElP1WD9rNPP2AM9rW4P2BA99[P2RL9rRPBAS995PBAS993PCON99C3PCON99@PDMFPDMF3PLOC9r<PLOC9r8PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%3iSCAN3SMED SMLtSMPL""wSPACAbSPAC3SPACAbSVER4.0SVER _]SVER,,Scal@Scan3TUBEA10Tmpr<UserphARphCH3phDYbigphQLcphTRphTRXPvc.[.u2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*P7C4%r5$fuu?>`.cH|fJmbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@E4KwMi;!DHXV,^:S=q16(+k*TQ^+9K-?S.LHSF{,ComMOx8yT,I)_|_n?_;?oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAh.iOWhbk1^wK[*oCf!`LY&'!"lC+/Ci2T6&3~.4MN1#G1NM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? sG9J31VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtAUDT{B1PtB1PtBCTS_hBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA (QDATA (Q>eDATA (QDATA (QDCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTabmFVocmFWO_GATCFeat//mGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANEPLAST~~]LIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS3qP1AM9rIP1RL9rElP1WD9rNPP2AM9rW4P2BA99[P2RL9rRPBAS995PBAS993PCON99C3PCON99@PDMFPDMF3PLOC9r<PLOC9r8PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT  #RUNT  ;RUNT  "&Rate @RunN""FS/N%3iSCAN3SMED SMLtSMPL""wSPACAbSPAC3SPACAbSVER4.0SVER _]SVER,,Scal@Scan3TUBEA10Tmpr<UserphARphCH3phDYbigphQLcphTRphTR