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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRUTTTLLckk&&&/0/999BAHJJRRR[[[ccbjjjrrrxxx~~~~~~~~~~}}}}}}}}}}}}}|||||||||{|{{{{{{z{zzzzzzyyyyyyyxxyxxxxxxxxwwwwwwwwvvvvvvvvvvvuuuuuuutuuttttttsttstsssssrsssssrrrrrrrqqqqqqqqqqqqqqpppppppppppooooooooooonnnnnnnnnnnnmnnmnmmmmmmmmmmlmmmlllmllllllllllkkkkkkkkkkkkjjkkkkjjjjjjjjjjjijiiiiiiiiiiiiiiiiiihihhihhhhhhhhhhhhhhhhhhhhhhhhhhhhhghghgggggggggggggggfgggfgggfggfggfgffffffgffffgffffffffffffffffffffffffffffffffeeeeeeefeeeeeeeeefeeeeeeeeeeeeeeeeeeeeeeeeeeeeeddeeeddedeeeeededddddddeddddeedeedddddddddddddddddddddddddddcdddddddddddddddddddddddddddddcdcdccdcdccdccddcccccccccdcccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc'((((()))))****++++,,,,----....////0000111122223333444455555666667777888889999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c5719f553db54e10bc09005b2f871fc2Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-03_09-46_16873130Seq18049137826_K1322_trnhfjmadera96-WellK1322_C11_Triumfetta_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z..//000/-++,/2577653211248=BHMRVZ_gpz-AQat ?r5h G9| WEj wGs:~[7}gXMB4" xT,iN2vkecceed`[VROMJF@90' ysnjfb_]]^`bdeefhkosw{|yvtsrrrsv{E,X = bqDuvGTU N  Q2hc7zaK9(~ywvxy{zwtpoptx|~~ytng_XRNKIFB<720/000/-+(%$"!"$%&&&'),0479851-+)((('''('&$!       "$&(+-/02469<@DGKNRV[`dhknqtyyvwwuoeZPHDBA?;60,)(((&$!        "#$$%&(*,/01100///0000/-,,++*('%$$$%$$#!                        "$&&$! !"#%&&%"  $*/3677665531/+(%#"! "&)***))))('%#  $(,29AKU_hpw}~{yxwuqkbXMC90("  !$(+./.-,+,/368850,)(*.37;?BEJOUZ_dkt~}yvsokgb_\ZWTOHA;6444430+%  %.9FQZagmvzqib\XSNIEB@ADHLOQSUWZ^beed`\WRNKGC@<9520..06?JU^bb^WPIEB@<71+&#"$'+05:>ABA><::;>?>:4.(&'*04640)!  !      "$&(*,-.../037<AGMQTVWY[^bfkqx+00.-2<KYdgd\RIA90$wpkgc_ZTLB7+!      !#%&&%$#! %.8@EHIJMRX_gnx #=Xs;j  /?Ur  4BGA4& &7LZXB{j_SB)\2 dN=2(vomotxxskc^\]_`aaabccb_ZTNJGHLS[dkopolhda_^^bhpyxrnot{yohgls{~vj\PHGKRY^_\VOGA;631136;>@?>==>ADFFGGHJLMJFA>>AEGE>2& &;Pbq}%<QdwR^Oy9JivvpiffcQ( !t- CpaL. 8}Hs2 !M  [Mx.&uN f i@,0O'zK ~snosxyul`VPNOONJEA@BFKOQSW\ckqtsplklptwwsme]VOKIJOU[^\WQLLQ\it{|ytqry{pf`_dmv||wmc\Y[_bb]TI?8310-("                                     !  "%(++*&#   #$%%&())&"#')*+-05:@DGHHGHJMOONMMPV^eihd_[]cny"5GU_fjns|$19:88<EPZ^]WOIFHKMMJFB>:4+yslaP;${ri_VNGA:2*#              !&*--,)'&&'''&%$$&*/4652-)'(*.232/+'$"#%*07=BEED@<9778;>@A@@ADIOSVUSQPPQRSVZalw~wrmid_YTOKIGFFECA><;;=>=93*!!#$#"!$&&$ !%'('%$$%$# %+/0,%                        &)*'#  #$%$"!!!! ""!                                   '9MaruaM:-%#%(***))*+,./010.+'#!!#&(****+-035530.-.02444458=BFHHFB?==@CFIJJHGFFHJLMMLKIFB<60+(),04540+(%%%%$"! !#$#                                                                                           "1>IPSQMHEEJPUWSI=3-,/4884/*''(+,-..03674-!  #*.0-)&&),/-'   #%$!    #$"     ),,(%$&)++%  '/48<@A?6*-=KSVWWX[]_adgkprsqmg`WJ:,!%-23.(!"&)+*(%!#*18@JVcmtvvvx~vi]SNKIGD?8303;FS\`_\[`m~yeO=329@C>5,)0@Rae_RE>AKXbe`UH<3-*)'#    0BYv@)FTXWWZ^^R7 iC.*2CWl&2BWp!pXT[bbV@#qU?.!xh]UOI@4&   !#$'-6DTdq{wlc\ULB91,)&$                               !!"""! !$&%# !$''% #&())('%" "" "$$#"""#" $(($                                                                         !%'())+/5<DKPTVZajv !&)*)'%%%%"vh[QIDA>;8631111/,)%%(.49:83.+,/5:<<951//1478864348>EJLKHDBBCDECA><<?BEGGGEDDEGHIGD@;7679;;950-+,..-)%!!%*./.+($! ""  !%+16872,%"!%*021-(#"%+28;:8668;?BB@>=?FSe~BtUCP5DD)g( "!  }{ywtqmjhfdb_\YXXZ\^___^]ZVPIA:6445652/+)'&%$##$%'*+*(%#!               ""! !#'*-.//14:BJRX^cir} /DYisy}}vpi`UI;-sg\SJB<60,)'&%$"                         !$)/58863126<BGHFB@>?@A>:3-(%#"                                                                                                             !#%'())(((()+-/121.*&#!          $'()('&%%$$#""!      !!"#%).2664/)#!&,14789;>CJPTXYZYYYWURNKIGEC?<:;=@CA<2'    !$).49>BEHKOSX]bfggec`^[XTOID?;:99863/+(((**(#  !(1:DMTY\\\]`ejoqpmjjlpsrk^PECK\q,-"hRA6/+(%# !#%'&$ "&))'# %+022126?JWfu ugWE2          "" "#"                  "#$$"! !"$%&&&''(('%$##$&&%#  ""!!##!                                                "$%%%%&'()))*+-/13469=AEHKMOSYais|%8Maw"1D\z=c&3?HLIB8-$$+140%mJ#R[6xrnkjihhijjkkkklmnnmjfa][Z[^acedb`__acefecbcfjoqrplihjnsx}}||}Dq(q>v*J^bU8|6| ] \   y o#J24R~XG  M5>|qg`ZSKC91*$  |unid`\XSNJGDA?=;::;;;;:999:::8632111/-*(&&(*++)%" "$$#  $(,.-+'$"    !$'*+,--,,,-./00000000.,)&$"""""! #&),.///0269=@A@?>>>?@ACEFHHIIIKMQUY\`djr{7Wv<dHm#GhymaQ:^-}R&|W6qaTJD?:50*%! #%&%#! !#%&'()*+,-.-,+*)))*+,,,++++++*)('')*,./..,+)((()+,,+*(&%%%%&%%$$$$$$#"    !$)-147<AHPYaipv}!/;FNTY]`dhlpqojbXMB7,! |rh\QG>72.,)'#            %()))*-29@FKNPQTX^elrw{~ytqnkgc^ZVROKGB<60+'%#!                     "&)*+*(&$!    %),-./1221-)$#(-11.("    )6CO\k}qbVOMNQRSRRSVXZZZYY[]`cddcb`^][XTQMJIHFEB>;86530-(%#"###"   #(+,+*)*.4<CHKKIFEEIOV\_^YSMIHJMOOMHA:4/+(&$#"         6WAUA,AAn}A-)_KB.bcpKB_3730_POP7_BDTv3.mobtermTCTAAAATTACCCTTTATCTTCATCTTGTATAAAAAAAAAACATATAAAACTTAAATTTTATTTCTTCACTCTAAAATTACCCTTTATCTTCATCTTGTATAAAAAAAAAACATATAAAACTTAAATTTTATTTCTTCAC,<JR^ny(2ER`x !)3FWhq~!.<O^cq{,<JR^ny(2ER`x !)3FWhq~!.<O^cq{                &O#4FHaH\5EWX4FTJ;! 6LFO@ ?^R+7U5& &8Z-B]FM"L)78%" /B  i(54  p? D  &$Cnt CLaoca%) @a8 B H %j (St[&B1#;cT`t p8JppQ{w]Z!@%Y Ae,!k E kFd4s} ! %/ ;V|pqF-jwsj\& 5UO AAAGGGGGGCGGGCAAGAGGGACCACAAATATTTTTTTTCGACATACTGGGAATTCGGGATAAATAAACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-03 11:29:36 -05:00AEPt3AEPt3APFN APXV2APrN ƼAPrV1APrX}}ARTNASPFASPt :ASPt :B1Pt 9B1Pt 9BCTSBufT ZCMNT ƅCTID`CTNMfCTOwyCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA Z^DATA ZDATA ZDATA ZlDATA "D.DATA "D..LDATA "D.rzDATA "D.DCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFWO_GATCGTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANE[LAST~~CLIMS!!ƒLNTD2LsrPaMCHNMODF ƳMODL3730NAVGNLNE`NOISP1AMFP1RLFAP1WDFYP2AMFqP2BAFFP2RLFPBASFFWPBASFFPCONFFPCONFFPDMFPDMFPLOCF)PLOCFPSZE`PTYPPXLBRGNmXRMXV1RMdN BRMdV1RMdXKRPrN&RPrV1RUND RUND RUND RUND RUNT  .RUNT  RUNT  RUNT  Rate 0RunN""6S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEC11Tmpr<UserphARphCH phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ƼAPrV1APrX}}ARTNASPFASPt :ASPt :B1Pt 9B1Pt 9BCTSBufT ZCMNT ƅCTID`CTNMfCTOwyCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA Z^DATA ZDATA ZDATA ZlDATA "D.DATA "D..LDATA "D.rzDATA "D.DCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTab&FVocFWO_GATCFeat..GTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANE[LAST~~CLIMS!!ƒLNTD2LsrPaMCHNMODF ƳMODL3730NAVGNLNE`NOISP1AMFP1RLFAP1WDFYP2AMFqP2BAFFP2RLFPBASFFWPBASFFPCONFFPCONFFPDMFPDMFPLOCF)PLOCFPSZE`PTYPPXLBRGNmXRMXV1RMdN BRMdV1RMdXKRPrN&RPrV1RUND RUND RUND RUND RUNT  .RUNT  RUNT  RUNT  Rate 0RunN""6S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEC11Tmpr<UserphARphCH phDYbigphQLcphTRphTR0ZNch>st_@U])%fD1,;0{U[%~#mXYV<{#-$>]/mU>p>X|.8As*5.@h23|=&u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbNfPZ2V~oLSHmR/+3p+M*JgbUA6WJ?eee1K/kx=gc1/kdSPaH~Cd*9+cUV&=o?eUKGM5J_32&L"gr[&Xo-,(B~bjOe:=fQS4MMZ^MG7G[k:[TWRIO@{$^(R[mPo1Cd}FxQPw#vpkMSvYTEl^Z-I-{0vTNiBx["#\+hfc{/99'jzc't:ez}Ii\a&?&=#GQbS9YH|O[nYnuPQh^5Q1V?yRl<`QV-@[3>>BZuGZ-p@vulc*{<=pjHOo&eG1Vcy[*oCf`y6n?2x`agv`kt3G/3t\#9/hV6T]==6X12O[n/bsDU5hf61FA>&oPWTRI1]f)yA@r9s_|g+*F}t.("P~fI2P&N+E/Gz^n4.6t}VX*os7}Z&[Eb4;,}s@dUa0u:W^$B!X`7.ykI0o6Gzv&1fu4WyYZFU5FS7&3-OT5ro{`N%7`h?&03ghW~^(';_/C]^HLl}5.X}=$~I!|sp#at.D>["r87xFl]xSbh#*l*L|n@gg-' 'DvddD\4A$a{|ds:MkOVc2(_CL,v.h}{ZE!]Rc{_@w,iP;YCWeq }X'>n~~2q>-kb+N4+07> YlGcRb>@iG-@yy3@=?]p&qN<;u4#k:X=<;s_|g+rapPj:,xNM,[*?BRCZcV]j?:{VxzTA=a:hVUDBW3h"mwT(AR,K0`JOGH\3?b<|7+n+iv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt3AEPt3APFN APXV2APrN ƼAPrV1APrX}}ARTNASPFASPt :ASPt :AUDT9B1Pt 9B1Pt 9BCTSBufT ZCMNT ƅCTID`CTNMfCTOwyCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA Z^DATA ZDATA ZDATA ZlDATA "D.DATA "D..LDATA "D.rzDATA "D.DCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTab&FVocFWO_GATCFeat..GTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANE[LAST~~CLIMS!!ƒLNTD2LsrPaMCHNMODF ƳMODL3730NAVGNLNE`NOISP1AMFP1RLFAP1WDFYP2AMFqP2BAFFP2RLFPBASFFWPBASFFPCONFFPCONFFPDMFPDMFPLOCF)PLOCFPSZE`PTYPPXLBRGNmXRMXV1RMdN BRMdV1RMdXKRPrN&RPrV1RUND RUND RUND RUND RUNT  .RUNT  RUNT  RUNT  Rate 0RunN""6S/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER SVER,,Scal@Scan3TUBEC11Tmpr<UserphARphCH phDYbigphQLcphTRphTR