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       89:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghij     89:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijv",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4977a14beebf45c0adccdf89d33c0eb5Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well"K1320_H9_Melampodium_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z)Dm<|dJ [  b) #x&X)y-1E6);AGMSY`hqf{0auleY^XSLN3IFCQA@@ABpCDFGVHJKqLMNNNMLKI%FDFAO=:X62.+F'$!X8DQ/3T4*`~;9e N~=GyU2r=O+/N{Lr$f!2D_ '8AJXo  = l  K 6 t v v &0@5UFnxcRE=8'3/+f&# w:!`a d ? ,     g DOeZ m N o  ] z ]%,2 6_9':;T;i;974 .U&  ;g(f j  r208&DmP3sWB76;@B?8.#1V9mkG$yQ${\A* %$$%#!#)29=;6/)%!  7XoXD3"     #;agM9)    "&('#"%'''&&(,17;=<7/(" "&*.0.*#      7d19&Y0 !!  &1;ADEFHKQV[_dku *5@LV]bdddehmt{~wpje^SE4!zof_\[ZUL>."             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FP}pYHY9`8pp8lpJwp<pTXjGb Lz:^ )SDBaKt0BMY-NI"u70CXrK=B";bW*j>NY4(QHX; x 'Ip7cp oQ ja2/ *8pjuKR5pu?UY)|{:Vw&RFj\!-fQX,4pHt iB aBT/7@6vk #nv'Z4o)wTL{@d8w VTU`y ScCZh`kV< 7e6:t"uUM""*,"{! _ %*h`]B@U?4)Fk^IW#M+"oK(nc1nEc5~DD<QGd&@q]67*Mj<Jos(/e(GfH=QqiOuVkd^6?)< m~25OxJ*%>fqrgF]O~K[.x02V\ _45Yh9vhXWP'VIa'j]a"!('sIA^@1vB$%\v| g[Q3U|OyDME%_:5os.1_ tkXAE,=rMhAAGGTGGCCCCCCGAGGGCACAAAACCCGTGAACCTGCTGGGCTATAATAATATATAATAATAATATACGGCTACCATCACGTGCCCCCCCATAAAGGGACATACATTATGGAGGGGTCGGGTGTCGCGTCCTTTAAAGGAAGGTTAAAGAGCAAATAATTGGAATTTACCTGTTAGCGTTGGATATTGGCTCGAAATTTGAACATCCCCAAGGGATTGCCAAAGGTTGTGGTGGTTTCTTGTCCCTTTGGTGTAGGGGGATGCAACTAAATACCCCGAATCCGAGCCAAGCATGGAGGGAGCCTAGGTATCTTCAAAGGATCTATGGGGGGGGCCAATTACCTATTTATAATGAGGCGAGTAGAAAGTTACTGCTGCTTCCAAGGTAAAAGCCTCAGATACATTCTGCCGGAAGTCTAACCCATTAAAACGCTGAAAGGAAATATCTTTAAGTAGATATGGAGAGTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:56:07 -05:00AEPt?YAEPt?YAPFN WMAPXV2APrN 3kAPrV1APrX}}3tARTNASPFASPtoASPtoB1PtnB1PtnBCTSy&BufT MGCMNT 3-CTIDMCTNMM#CTOwM7CTTL 3$CpEPDATA@DATA@DATA@DATA@DATA  >DATA  DATA DATA (DATA ?TWVDATA ?T֪DATA ?TUDATA ?TRDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FWO_GATCGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~wLIMS!!3:LNTD2LsrPaMCHNMODF3[MODL3730NAVGNLNE`NOISTOfScVX P1AMg)P1RLcP1WDjP2AMr!P2BAuP2RLnyPBASVPBASTPCONaPCON_PDMFW6PDMFTPLOC\1PLOCXPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL##WSPACAb SPACTSPACAb SVER4.0SVER ySVER,,2nSatdVXScal@Scan@TUBEH9Tmpr<UserphARphCHTphDYbigphQLcphTRphTR.This is the confidence range *ABI_Limits..FVocAEPt?YAEPt?YAPFN WMAPXV2APrN 3kAPrV1APrX}}3tARTNASPFASPtoASPtoB1PtnB1PtnBCTSy&BufT MGCMNT 3-CTIDMCTNMM#CTOwM7CTTL 3$CpEPDATA@DATA@DATA@DATA@DATA  >DATA  DATA DATA (DATA ?TWVDATA ?T֪DATA ?TUDATA ?TRDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTabFVocpFWO_GATCFeat//AGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~wLIMS!!3:LNTD2LsrPaMCHNMODF3[MODL3730NAVGNLNE`NOISTOfScVX P1AMg)P1RLcP1WDjP2AMr!P2BAuP2RLnyPBASVPBASTPCONaPCON_PDMFW6PDMFTPLOC\1PLOCXPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL##WSPACAb SPACTSPACAb SVER4.0SVER ySVER,,2nSatdVXScal@Scan@TUBEH9Tmpr<UserphARphCHTphDYbigphQLcphTRphTRqk+VxnD\jE@r9,V\Nz{rT'_T%`C/&m|0locKp'" H,.9)%"ef!>b7T4lpz?Wzi b!a72BZ|7W.`4A,/|8H15KK;x+;(8W)\lZh 8<"&eG1{?v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r(#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`@){,@Vk1$#=4{,\04^!f+tNheeDD:\Ao!:Z`+a6:!.tkSY4E2rfJX|PzMWhceS-bb3SvrsPo"`dbp~lV}sT!lZbCd]E>]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72Wer]LD_!lEH|x]0oPGlOG!>RE.$2<2oYWA8aM|T9}OFBF^gmbXc+-I]Bd $D%#u^;i^NDF4+3{y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H%R7M@ig\[g!(Nn %v`to?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQLAEPt?YAEPt?YAPFN WMAPXV2APrN 3kAPrV1APrX}}3tARTNASPFASPtoASPtoAUDTTB1PtnB1PtnBCTSy&BufT MGCMNT 3-CTIDMCTNMM#CTOwM7CTTL 3$CpEPDATA@DATA@DATA@DATA@DATA  >DATA  DATA DATA (DATA ?TWVDATA ?T֪DATA ?TUDATA ?TRDCHTDSamDySN 3Dye#DyeN3DyeN3DyeN3DyeN3DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 2EVNT 2EVNT2EVNT2FTabFVocpFWO_GATCFeat//AGTyp!!2HCFGCEHCFGHCFGHCFG==InScInVtLANEALAST~~wLIMS!!3:LNTD2LsrPaMCHNMODF3[MODL3730NAVGNLNE`NOISTOfScVX P1AMg)P1RLcP1WDjP2AMr!P2BAuP2RLnyPBASVPBASTPCONaPCON_PDMFW6PDMFTPLOC\1PLOCXPSZE`PTYPM?PXLBRGNmMRMXV1RMdNDRMdV1RMdXERPrNLRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 2RunN""LS/N%TSCAN@SMED SMLtSMPL##WSPACAb SPACTSPACAb SVER4.0SVER ySVER,,2nSatdVXScal@Scan@TUBEH9Tmpr<UserphARphCHTphDYbigphQLcphTRphTR