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O           !"#$%&'()*+,-./0123456789:;<=> !"#$%&'()*+,-./0123456789:;<=>v",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 7059fdd941d94f5285cf82643e093540Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well.K1320_H3_Liquidambar_styraciflua_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7zH," l k q  gzO #J&*/D38=CBIZPHX`is"|Kyq<841-*'$H!@sngz0rL,(YX6n  0 d AO!Q)17972S)6Q $7  <avn\ m F > ] h:qjK9*f"hH& Cm,\nP2 y>[#|xqi`XPH>4+&&*,+$    %O,C\|}'j'   (+'          6nm7= %'"       "&!3LZ[RE<=EP\iV.t\`2 "&#    !   !%-/-($%,7AGHD?<?HVfsywnc]cu "$'+3=GMOLKOZk|ziZQQV[ZRC1!  z]E4-/8DMPMD8,! 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'/1.(#            '(#+462'   #&%! *00)$'#            !#           #%&%!      &)& %(&             $&$              $&'&!         )5>BB>6,! $*'N~@_Ay>A\A#]KB.bcpKB_3730_POP7_BDTv3.mobtermACGTATTTTATAATATGTGGCTGCTACTCCGCCCCCTTTACTATTTTAATAAAAAAAAAATTACGTTTCTACCGTTCATCACTTTTAAAAAGGTTCTTATCTTATTTATGAGATAGAAATATTAGGCGGAAAATTCCAATCCTTTTTTATGTTAAGAATAATAACTTTACAAAAGATTGAGAAGAACATCACATATATAAATTACAAACAAATTAGTACAGAACAAAAGTATGATACTCAATCATAAACCAACCCATAAGAATACTTTTTCTTAAGGTGAAAAAAAAAACTTATGTAAATAAAAGGCTACTAAATAAAGGAGCAATACCAACCCTCTTGATAGAACAAGAAATTGGTTATTGCCCCTTCACTTTCAAGAACTCGTATCCACTAATAACAAAGACTTATCATTTATTTGATGATGGTTCTTCAGCAGCCAGGAGGAGTGGGTGTTGTTATGATTATGACCATGATGCAACAATGCGGGAACGTATTTTATAATATGTGGCTGCTACTCCGCCCCCTTTACTATTTTAATAAAAAAAAAATTACGTTTCTACCGTTCATCACTTTTAAAAAGGTTCTTATCTTATTTATGAGATAGAAATATTAGGCGGAAAATTCCAATCCTTTTTTATGTTAAGAATAATAACTTTACAAAAGATTGAGAAGAACATCACATATATAAATTACAAACAAATTAGTACAGAACAAAAGTATGATACTCAATCATAAACCAACCCATAAGAATACTTTTTCTTAAGGTGAAAAAAAAAACTTATGTAAATAAAAGGCTACTAAATAAAGGAGCAATACCAACCCTCTTGATAGAACAAGAAATTGGTTATTGCCCCTTCACTTTCAAGAACTCGTATCCACTAATAACAAAGACTTATCATTTATTTGATGATGGTTCTTCAGCAGCCAGGAGGAGTGGGTGTTGTTATGATTATGACCATGATGCAACAATGCGGGA2=\k%;GZit -7@HPWes/<GO[co{ !1>JQ_jt +6CP[es $.;GTbn{ "0<JVbnw)5BO\fs~    ( 3 > J U b n y    * 6 C M Y f r    % 0 = H S ` m x    ) 4 @ L X d o |  # 0 ; F Q ^ j w  %1=JUamy +6CNZgt(5AMZeq| $1=HS`ly*7BNZgt'2=JVco{ "/9FSbmy  ,9GS_my#09DP\hx+6DP^2=\k%;GZit -7@HPWes/<GO[co{ !1>JQ_jt +6CP[es $.;GTbn{ "0<JVbnw)5BO\fs~    ( 3 > J U b n y    * 6 C M Y f r    % 0 = H S ` m x    ) 4 @ L X d o |  # 0 ; F Q ^ j w  %1=JUamy +6CNZgt(5AMZeq| $1=HS`ly*7BNZgt'2=JVco{ "/9FSbmy  ,9GS_my#09DP\hx+6DP^ */$ ."      ;>;>;>+13+(-#    138$*816$'+18$+#600881    ".0->>>>6>>;;>;;,".++81;228868;;>>>>>;..286.68+66">>;;;>8>>>00"""1)32;;;608>;;;>(6;6*02;;>6;>;;;;>>;83322.223228>8>6 >;.>8;;;;8#&+#" 6.6;68;82 &+;288>>>;861(";" 008;>>;>>>>>>2;;;06;6;;8;;88006;33'2&";8866;;>>;.0;;8>8>>>8;31;88.&.>6;>8+1.00$   ""       "                */$ ."      ;>;>;>+13+(-#    138$*816$'+18$+#600881    ".0->>>>6>>;;>;;,".++81;228868;;>>>>>;..286.68+66">>;;;>8>>>00"""1)32;;;608>;;;>(6;6*02;;>6;>;;;;>>;83322.223228>8>6 >;.>8;;;;8#&+#" 6.6;68;82 &+;288>>>;861(";" 008;>>;>>>>>>2;;;06;6;;8;;88006;33'2&";8866;;>>;.0;;8>8>>>8;31;88.&.>6;>8+1.00$   ""       "               8E4:cb L:[4?+"A8F2`\8A;&P%.!DKB <O)C!L"_@M@L/*=/T R;Z=6Q93XWEF/ 6K#< 4 FL+@J&A cIY!b CUQ4')a:[7>DJ4HH6A VBG\^ZQ)Ca]FUOQ4R#U+ ]:54X[,.$"E5:#/L996MBEM!" -J'"@+;.G`a[ Ja3A4+d/4)&QB$@]PHJ(X O@ .NP!cT\ #8";QKFPCGV$X:&QKZU2" NORG:6`7G.B/ .@&Z#Q]Z B<D +Z='L R[G6T7DF1W>=BBO]JQ#MZ+7]]5_XKKNd\CKY0b3/0<Z9]XRJ1;(J%?OW=YM/3#=a9#!]#; &3P ]J%+%_"ZH<%!?,-d1Bb*I,2^&8>S6MRb80__+1 _%O3}E'v=1P"]Zm}QZ ? f "k4}P/A-P& _F   CvmewR }kNd>TDEq|1 ^E\D.}SR_BZ! 0yG9 OsG2ZqZkO&",RS-8G <?h/=G79%AuC &{;*_X)Kt.Jej *IB3!r W?xH[|7?.OP2=A8)[4C <Zc+$ &c J$[GTJ$>44A  5e`s@~.IjN[mfN@$'myO^:3F h! $b  dVOnIks_BoiN5> B]#&#1NgY]5 2A$.YO? >. 4~6   #-l#"* /Q.$/ /,p;3?&  "- !=/2,/GUJ *_7  ,6%P;36[Y-\%xIkh/<KEiF<8.h(J %U+n h`Bp<e8z-2k.]CjnZCSZp<"(dE\V@*}S'd&t7]!R":a_5Fp+ h|'^od+(stxA7VWpK]' +$P$2,Ajv|,alVEx}X~x8~Z4io$h YB$PC?p?2Z+t#?s*pg8Qp-%Y vIR 8,z `g4eRaY&Redvq&P/:M6-<Vo=@rnE+Y`nDRVR/49,X.LO:OSfI|/Ka Dt:B7QvDH3SgHiOi|w2af/$0=zXVt`WYLmcH,d<n;BGGAGGCCCCCAGGGCCAAACACCTACACTGCATGAACCATAATCAAACCATGGTGGTTTTAACGTGAATCCTGCAAACCTCTCCAATTGTGATGGGCGGCTCAGAAAGAACATATCCTTCCAACCCTCAGGGGAAAACCAGACACGGGTGAGCGCACCATGACTTCCATGCGCTAGAATGAGGAGGGCGTGACAGATATTCCCCACGGAGGGGGTACTTTCTTTTGCGCCGGAGATTCGGGAGCAGGGGGGGGGGTAGGATTATTCCAGATCATGTTGTCCCCGTCCCTTCATGTATCTATTTGTCTCTTAGTTCGGGCAGCATTATTTCCTTTCTCGAGATACCACGACCGAGATGATAGAGTGTCCACACACACGAGCACGTATGTACTCTCGGCGGCCATAGCCCTCACCTGGAATAAGAACAGTGAAGATGATAGCCTCTTCAAAAGAAAAAGGGCCGGTCTTTGTACCATACTACCAAGCCAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:39 -05:00AEPt=AEPt=APFN ViAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPtOASPtOB1PtNB1PtNBCTSyBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA ?VVrDATA ?VDATA ?VUDATA ?VtDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~x=LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISSOfSc8 nP1AMgP1RLcEP1WDjP2AMrP2BAvUP2RLnPBASUPBASTPCONa]PCON_uPDMFVRPDMFSPLOC[PLOCWPSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL//V#SPACA_OSPACSSPACA_OSVER4.0SVER ySVER,,1~Satd8Scal@Scan@TUBEH3Tmpr<UserphARphCHTphDYbigphQLcphTRphTR^This is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN ViAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPtOASPtOB1PtNB1PtNBCTSyBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA ?VVrDATA ?VDATA ?VUDATA ?VtDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTab!FVocFWO_GATCFeat//GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~x=LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISSOfSc8 nP1AMgP1RLcEP1WDjP2AMrP2BAvUP2RLnPBASUPBASTPCONa]PCON_uPDMFVRPDMFSPLOC[PLOCWPSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL//V#SPACA_OSPACSSPACA_OSVER4.0SVER ySVER,,1~Satd8Scal@Scan@TUBEH3Tmpr<UserphARphCHTphDYbigphQLcphTRphTRaB$T9F_z"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|?92[N:VhzRrJ1Mo0b>k_8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH:/ULhwvKQY6|" ;t(ua]&j,6|b-mQla lk)cT(6 UYpVPE$v fMOiiX>VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitGy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:.z9W-y]l0Uo~e8HJj`$dUW7.m/J[Y^%X\HifcJkh<;!xpB5oMDHlq3[m4:7{%_hj}-Q:;~`}'vVE$;;OwV-O$![Bwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>