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()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMN()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNv",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4c84b275751d4f09aad41e3da70d5229Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well(K1320_G4_Hypoxis_decumbens_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z}},?Uix~~+Jf~O o?v: V % .X""'D,_2)8?`FNyV`itjzXose\U/NIDm@>3<<]=0? 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" (.  4&/#  ! 4!    !! % ! % %         "  2$         )Z"aQ b= I>HR<39=-Sc QY#H=Cc`-["@M/ GJ [1H+4N`**LBUJVa"$c! 2D)D4)^X DW3\UE9,I ',:Fa PUK #V(338A]S \Y %W(@%VY40H,.%,%!` 4[ Q^[ KX-7`=71B @< c#D_c;S D-_N 7'". +DVOd# T(';^` ?8%I7YAM& N0=C@%`Q_6E R-_?0F0$Q( MAE 31a'7%R@V^bJ.5!2ZAX]$B1?<I,&<,#\Bc Q O?S`\"41=]1'KA  0*31'XP,X##M]C'Zb^=*VRAP9Dc)bU6O>a]Z9!^&Y<B$S.68-2 6 OI<  O?VR J@DH3cLEY- R Z% Z:* M9 Z/!\MU%2Z2@&L Jd<9a 6ITM\G(&P2?#)(31#A22GF= 23N32*:QW '&T?QF2$K2]$]U5=0*W2*:c%D702-[ 2*: VI *:W` VA+JP`c$ QO+#*U+RGbM?BZ:> 2EDmk*t i,O6'D_ 27,`:?`T/Vb*  uS-D/PM c `! `QX*bF '8f;* \2Q$x j?P&z4M|Q-mSz.+zarz8\:lNng@mS7?Pc<A$kde" s1;IsN 4z.d\5l@z|z2onr,Z,v*}=A^Hfh(8}iox#zt"/Az4E9 %f~p w(g#!.~^5p`PEF;%8,mPTV#q=PM0t<THbtw,v t0>EXF]{!kJZ_f7Om:ZDVvcS6  |  K, I! 4LVflD8XURu) ?"lX n$+@vQim9S  iV@L#c(P);pVZ!7`%D+L0W-'8LZ*_#8d))\*U%"ul8+J=9-&*"%,x$ 407"X 3 $96$D &  " I!,; [/ +uXU=R$  4+  F!&A#<.+"&t&L -*^=!%) *= ._U7SjH"s!^.j^33((c4!)O666&66ff6CC66:CCCCl6QCUUf6U6666+6656F-|yyyy_:__y___h__UOOhyO__HoI_OhO__OO__H!py]p,;qlsQG0}I0%s-\pGw{KDrs\,w )/|E?d9Xa9 r*g8dvLt ($#.y_qHHsL 7+}Lx)W{y{Mcd8F|85 >d6K87 n8R?R]X_0wR!AHy5BpP\()/c .dxdXX'FL3WinDBakIt~8_'8#5&^h\ L7p ~a8Ep}t)owdd}34PpowYLt{'DLp 8hva ppj\fz_0jpcp#,p%!G81Z;][-P(K_\# { )9xr^$6varF:X2@6bG`,s u+  "w_zsRt}|6K.m _|wealq=`z:_RJJXDgq"{X E k'!. LKs>dJ1Oz+lOX77/ s7-KKwAsx8I)*{Rd5  #sO5lkr"'Q{A; }@UzS6e |/U]:Wx=# G[f uJ-J[ xF[oO\d}lU\kd0DU>F$%vKU2z2Ji0Qhe>>Js`#CUA' 6}UXu=^*FkY0J3<__3{j2+R3X$p;:  lq]XVF:ij{(34?cE8q CCTAGAGCCCCCCCTAGAACAAACCTTGTAATATGCGATCATCTACCGAGGGCTCTAGAAGTCCCTCACAAGACCACCCATTTGGGAGGGCACATAAAGATAGGCATACTTCGCCCTCTAAACGGGGAGATGTTCAAGTTTGCTCCAATTCAATTATAAGATGACCCGACGTGTGCGAATCCAAAACCTCCATATAAATCCATATTTTTACTTCCGTAGTTTGTAATATATTATCGCGCCGTATTATGCTTTCATTCGATAATATGACCAATACTATTAACATCAACGATTGACACATTAAACATAAATGTGGATCTCAGGGGCAAATGTACCCGAATATCACCCCTTCCGTGGAAGTCACACATTTGGAAATTTTGCGCTTATACTGCCTTTCGCTGGACTTCGAGGGACGCTAGATGATCTCGCTCATTTAATAATCTAACACTGGTGTCTAATGTGGGTCCAATCCCAAATATCTAGCGTCATCGGACGATGGGCCATGGACTGCCACCTCGTTGAGTGACATACAATTAAATTTAGACACCTCACCCATTCGGGTAAAGATTGTAAAAAACGAGCAGCTAGTCTAGAAAAATTGTGTGAGGCGATACTCAGACATCACAACAAACAGAGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:41 -05:00AEPt?AEPt?APFN VcAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPtGASPtGB1PtFB1PtFBCTSBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA ?TVlDATA ?TDATA ?TUDATA ?ThDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISSOfSc8 nP1AMzlP1RLzgP1WDzqP2AMz{P2BAzzP2RLzvPBASzzVqPBASzzSPCONzzeMPCONzzbPDMFVLPDMFSPLOCz]PLOCzXPSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL))V#SPACAbSSPACSSPACAbSSVER4.0SVER SVER,,1~Satd8Scal@Scan@TUBEG4Tmpr<UserphARphCHSphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN VcAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPtGASPtGB1PtFB1PtFBCTSBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA ?TVlDATA ?TDATA ?TUDATA ?ThDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat..GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISSOfSc8 nP1AMzlP1RLzgP1WDzqP2AMz{P2BAzzP2RLzvPBASzzVqPBASzzSPCONzzeMPCONzzbPDMFVLPDMFSPLOCz]PLOCzXPSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL))V#SPACAbSSPACSSPACAbSSVER4.0SVER SVER,,1~Satd8Scal@Scan@TUBEG4Tmpr<UserphARphCHSphDYbigphQLcphTRphTRGP58Jr7y!{_a1z{,c]n{uH<6#RLk5/>RbS4}(FtSEH[!PYow.mU5s%+ .*;=8Es{jGyZ.T3d8A2r6fgh`U*{Zq9FyhCebT8Poo=[/19~{2A~&!sR(&^iCq=dGE^E:DH6[~=7?xh?'kT'_C:q|4G,fX-N&Wv'i2{M~V9s%LXA8%3z|M}nmt+V8!0.9,(B~bjOe:=KKR"\3|-bWF'zP}6Ja!8UY59D4I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6D/N1RVkg#WLv'UCz-kNH"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(IM_C4VwvrUM$h<+;"EH(,OmJpdZ8G72W3Un^=M\![6iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/AEPt?AEPt?APFN VcAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPtGASPtGAUDTB1PtFB1PtFBCTSBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA ?TVlDATA ?TDATA ?TUDATA ?ThDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat..GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISSOfSc8 nP1AMzlP1RLzgP1WDzqP2AMz{P2BAzzP2RLzvPBASzzVqPBASzzSPCONzzeMPCONzzbPDMFVLPDMFSPLOCz]PLOCzXPSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%SSCAN@SMED SMLtSMPL))V#SPACAbSSPACSSPACAbSSVER4.0SVER SVER,,1~Satd8Scal@Scan@TUBEG4Tmpr<UserphARphCHSphDYbigphQLcphTRphTR