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"':WatN ' !&4B[pgmE.  %@Ci}{_.3?kk}J+ 'GWh~j`H  9IgG572  *8:4@;DfetkPm^mymp~nK8)#!   5\yidR:+/NYeI5 08L\[LB=%  "! =.9.3JDA/ITAU&;FI5'9!A-%#*'&&   $.)- /2&/ (&8$-701/ #/ 0%  !"!"8% 5(F'+'8-$<"'#:  :J6 && &")(&*%" %/ #() ##/</- !"  ,+)> $    # *0,57"46E*8)! &=8RKkejC7)  B1SjTY:&71*!99Vw|eNcVTdq]y^:O+' /H`cyIMIX}uc8'$  -:v+-"  Cosp\EpR>-                   !"#$%&'()*+,-./0123456789:;<=>?@AB !"#$%&'()*+,-./0123456789:;<=>?@ABv",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 239825765ce1434cbbd865565e4e5976Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well)K1320_G3_Agrimonia_pringlei_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z7Vt.} nT; j % "k z#',T18M?GaOXbn|{zOowe]5UNHASTKgC4:7130D-T*'u#] +:(IW3^_ WH7%s$ $ -u^#q( { 0:h"b6aF1 tej : z ; . ^ [ " 7hFChwg@ kLx@<WuqG [$"'%! 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K<Z9@O3D&907&K P<H?!4^AY/_ J^Z>57 U`R;A> `E D[HV]SR@$"b1 c9dZ-GNbTHW])];8V1 &1O<ACA`!'[K3d 8A Z 2=K CIXOQ#T6ZH6S&29.T$8[ 2ZLM#;);) S?K0I^0[4)H cSI ?M [S@R)2J11F5 <+ @*QT( KO YYd2U $6QJ^Z2?:M7<% N2<b3NB _R92XIK]Qo" xB/2 O(&`_/ @d`6T fjje)yC {+WuB@po^ w +,{ V oX + .&iC!wn@J I~LiI][_l _|j5 +%y L#[<HY  =;*<8f~x Wru I ,zj Ek277aJ i(; S"gK71Im<L`@ \}%4DC(WgeKkKGKkN)4Eb'_Svr I"!+  MkO]g  6q *U#YvOBx 7?*y6(A1 AG X  jVD6]ahn2PHPBa 6$ YcJ?OX4=My, :0+"44 MG008GC'< 3    #%)1 C55P-*,!.% *9 <#   , .  v7"B(ST4-" B+!yS<U+ ->+9.0##CGActpCp!5 p:pp{xxxp?r1nLp)~NK ,de,hE8(gRG`A)C}zTm.dWaadv5ByrdKO8 ,]], |K,Yt>q~R)U H*:Omb-+1_N42JDsdErs%:6d>Y; 4;pv]w(:pSwod1+>dCz $<Q:6DOi>k%J:#>&a1|S=!=  }6@TV]z)oy^@{aa84n2TvX+Uo7KuLI9s)we=UmvR7gRd@I{1 L0f)WgmFvAcD~-b8"c s5&QWyVe"+R VaDyy~]uWP&`]sc8lh2F`a7\Vi-D2d=0_}gvYo OFu2"}-lnc?M\qO zg7tSfmde  _}sc3J;tGYjHtQDYO/ ^KrHgEd)%\ AAGGGAGGGGGCCCCCGGGGGGCAAACCCCCTTACCACTGAGGTTACATACAATGGGAGTGGGGGACGGAAGCAGGGCTTAGCGGGGAGAACACCTAAGGTGGGGATACACGGGCGCGGGGGGGGGCACGAGCCCTTCAGGGGGGAGGCGGGGTTGGCGAAGGCTGCGGGCGCTGAGGACTAGTTTAGTGAAGGGTGGGGGGGGTGAGGGCCCCCGAAAAGGGACTGGTGGGGGAGGAGGGGGTGTTCGTGGCCTGGGGGGGGGAGAGAAAGCGCAATAGGGGGGTTAGGGAGGGAGAGGGTCGTGATGGGGGATGAGGGCGGGGGTGGCATGTTTGAGTGTTGGAAGGAGGGGGGTGGGGAGAGTGAAAGTGGGGAGAGAGGAGACCCTGGAAAAATAGGGGTTTAGCGAGTCCTACACTCATAGAAAGGGGAGAAGTAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:40 -05:00AEPt=AEPt=APFN VDAPXV2APrN 2[APrV1APrX}}2dARTNASPFASPtNASPtNB1PtMB1PtMBCTSoBufT L7CMNT 2CTIDL CTNMLCTOwL'CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  .DATA DATA DATA 'DATA >}VMDATA >}DATA >}QDATA >}ϟDCHTDSamDySN 1Dye#DyeN2DyeN2DyeN2 DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~n[LIMS!!2*LNTD2LsrPaMCHNMODF2KMODL3730NAVGNLNE`NOISMmOfSc4 ^P1AMr^P1RLr[hP1WDrbLP2AMri0P2BAlP2RLrePBASOYPBASMPCONYPCONWPDMFV-PDMFMPLOCrTPLOCrQPSZE`PTYPL/PXLBRGNmLRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%MeSCAN@SMED SMLtSMPL**VSPACMSPACM}SPACMSVER4.0SVER oSVER,,1^Satd4Scal@Scan@TUBEG3Tmpr<UserphARphCHMphDYbigphQLcphTRphTR4lThis is the confidence range *ABI_Limits..FVocAEPt=AEPt=APFN VDAPXV2APrN 2[APrV1APrX}}2dARTNASPFASPtNASPtNB1PtMB1PtMBCTSoBufT L7CMNT 2CTIDL CTNMLCTOwL'CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  .DATA DATA DATA 'DATA >}VMDATA >}DATA >}QDATA >}ϟDCHTDSamDySN 1Dye#DyeN2DyeN2DyeN2 DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTab~?FVoc~.FWO_GATCFeat//}GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~n[LIMS!!2*LNTD2LsrPaMCHNMODF2KMODL3730NAVGNLNE`NOISMmOfSc4 ^P1AMr^P1RLr[hP1WDrbLP2AMri0P2BAlP2RLrePBASOYPBASMPCONYPCONWPDMFV-PDMFMPLOCrTPLOCrQPSZE`PTYPL/PXLBRGNmLRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%MeSCAN@SMED SMLtSMPL**VSPACMSPACM}SPACMSVER4.0SVER oSVER,,1^Satd4Scal@Scan@TUBEG3Tmpr<UserphARphCHMphDYbigphQLcphTRphTRc( !j rh[Z66YM'KvT+F4:H[);@#PA+eg[gUGGN*+j:xUIc.jCxO{VZHngM0xW#nZE35ux2]DM6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(Y`N!:X>zt %v1${NW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`ht1A'obU2kR@ef&e}hw@q}%Q`ALIghJ*(k;:|e}OM *R@_>j23T6Y/Xh]'p8Npdo(ktnP_I[Dd@2g|5J_@A[]"p64y^M_9Ui|2Z/qNV4]uCS\7X GGg?v!YH}&8)TK72K`rxPw% e M]Pz%y;|>EYWC.%o,n`!39G,RS_%ssaP8/GC.JuCglxsQlnWH%`WZ\9~#Mz~&= *r,`'#O`5Irt8dsh_Ox[L_Pu;,kG<7UXp#63P!gFS]T'_C:rF(5,f *",lp^ksL}CP~([%$G`pc_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]H6X+9mMyTAFU5eY\`;w>xPqL6`nCRNXo~%y>AEPt=AEPt=APFN VDAPXV2APrN 2[APrV1APrX}}2dARTNASPFASPtNASPtNAUDTB1PtMB1PtMBCTSoBufT L7CMNT 2CTIDL CTNMLCTOwL'CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  .DATA DATA DATA 'DATA >}VMDATA >}DATA >}QDATA >}ϟDCHTDSamDySN 1Dye#DyeN2DyeN2DyeN2 DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTab~?FVoc~.FWO_GATCFeat//}GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~n[LIMS!!2*LNTD2LsrPaMCHNMODF2KMODL3730NAVGNLNE`NOISMmOfSc4 ^P1AMr^P1RLr[hP1WDrbLP2AMri0P2BAlP2RLrePBASOYPBASMPCONYPCONWPDMFV-PDMFMPLOCrTPLOCrQPSZE`PTYPL/PXLBRGNmLRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%MeSCAN@SMED SMLtSMPL**VSPACMSPACM}SPACMSVER4.0SVER oSVER,,1^Satd4Scal@Scan@TUBEG3Tmpr<UserphARphCHMphDYbigphQLcphTRphTR