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***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 94cbb98bab0e4221aedc0a79e0a8f6e8Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well'K1320_D5_Chaptalia_nutans_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7zYRz  + tl%!V%).r39i?9E@KRoYajxs||$sqke`V\1Y 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OoVN6rkQ\=>'ii/ szb);~9MNSfh f:(n1Cp$V+.?  7{9&} DF#J :4z0Gd=gT H$ 4&696=yJ9YtX=JL T3Xe_FS$b;V!uN(T;: >'!$&0j(cS%D2J&  0  !: !$?+  oF    fJ 7&2  %2<$ )*#B *<#726&+. 4,  %)- q5""MJFC&%c76 %B!Sg|spp'pZ, kyAzo*-p(E dS`2/!.#'Kv/RpEytpPQTA'^:5CHh1K{^z #1;:(Y j98RZ,nuT!YtpIF&%F,BV{pH1pO]pppEe Ap|+Z*d y=QTOxo"XTzIAy7O4 0Z 1o Gun$D,15> 'X40}: *wY1+ `->I6k .P.X:no~Z /~.?@})]-6*-12?KA= -#0K%X/_&B+{Qyt[;(I2* )8TRoT-#)%4'+#-#BTS(A4V(/FG7'N>$]@"1(lA&.+1ki)YdJA=* %-.n0UG)=.%51D3^(Z2'JAo#gH5O(T)&2.F4Nm`90=b:2 -hH`[(`#QM<h$PCB $@FR sB#H69C=+=u6VKArFxMa4=ey;Rm,1lyOjY=&g7>BO'z>s.e*$LL7\6Y1DH fJCP@X@7u'c^I`j~1PFFWlP{q=_X3[BeP1 2L`hv_Qk'y(Q$(rd   Y|Y&}( )QGAGGAGGGCCGCCCCAGGGACAAACCACTTTAACCTGCGTGTCTATAATAAATAGATAAGAAGCGAGCGTGATAATATGGGGAGACCCACTTAAGGAAGGGGAGAGTGTAATAGGGCGCTCATGGGACCCCCCTTGGGGGGGGCCTGGCGAGGGGGGAGACCGGGTGGTAACGGGCCTTCCATCCTTCCTTATCGTCGGGCCAGTTTCCCCCACAGGTAACCACCCGGGGGCCGGCGGGGGTGATTCGAGGCGGGGGGCGGGGGTTACTATGTTTTGAGCGCCCTTGAGGGTATAAGGGTAATTTATTGTTCTGGAGTAGGGGACGAAGTGGTTTTTACTCCTTATTTTTCTAGCATTGAGACAGCGGGAACCGCAGTGGTTTTCGAAGGCGCGGGGCAGAGAAGGGAAACCCAAATCCATGGATAAATACATTGATAGCCCGTACGTCTATAGTAAAATATAGGGCGGTCGGTTTTTGCCTTACTACAAAGCGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:50 -05:00AEPt>"AEPt>"APFN V:APXV2APrN 2SAPrV1APrX}}2\ARTNASPFASPtBASPtBB1PtAB1PtABCTSyoBufT L/CMNT 2CTIDLCTNML CTOwLCTTL 2 CpEPDATA@DATA@DATA@DATA@DATA  &DATA DATA DATA 'DATA ?&VCDATA ?&iDATA ?&TDATA ?&ӵDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~wLIMS!!2"LNTD2LsrPaMCHNMODF2CMODL3730NAVGNLNE`NOISROfSc3 ZP1AMfvP1RLbP1WDjVP2AMrP2BAuP2RLn6PBASUPBASSPCON`PCON^PDMFV#PDMFRPLOCZPLOCVPSZE`PTYPL'PXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%RSCAN@SMED SMLtSMPL((USPACA\)SPACRSPACA\)SVER4.0SVER ydSVER,,1VSatd3Scal@Scan@TUBED5Tmpr<UserphARphCHSphDYbigphQLcphTRphTR,This is the confidence range *ABI_Limits..FVocAEPt>"AEPt>"APFN V:APXV2APrN 2SAPrV1APrX}}2\ARTNASPFASPtBASPtBB1PtAB1PtABCTSyoBufT L/CMNT 2CTIDLCTNML CTOwLCTTL 2 CpEPDATA@DATA@DATA@DATA@DATA  &DATA DATA DATA 'DATA ?&VCDATA ?&iDATA ?&TDATA ?&ӵDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat//GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~wLIMS!!2"LNTD2LsrPaMCHNMODF2CMODL3730NAVGNLNE`NOISROfSc3 ZP1AMfvP1RLbP1WDjVP2AMrP2BAuP2RLn6PBASUPBASSPCON`PCON^PDMFV#PDMFRPLOCZPLOCVPSZE`PTYPL'PXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%RSCAN@SMED SMLtSMPL((USPACA\)SPACRSPACA\)SVER4.0SVER ydSVER,,1VSatd3Scal@Scan@TUBED5Tmpr<UserphARphCHSphDYbigphQLcphTRphTRTrU]wfg6v;(vJ4;iD4ZI4$%\e]yaQ>2nxHBmHle8hmYh^n_k@0{RRV3?wrmJGLbva].p>x'[IoOoq4^M1}GVRI<672_4CF_E[UO')JRr+4#Mc]_^ave>uDs5iqbyMimE%ToN6|1:#<'r{?:TA2oz:4JA^V\h}c%kU?-}U&0;S3ZfYUgB\(<({]oT2mt1A'obU2tRHef&e}hw@q}%Q`+N@c'WQwK m}{H1}z3/onxd:}ml]q:jJRWO[@*!$T]_`#?Q{6Jn`BT]{?v2FKeS5~i2oru-<7X_ Wl*ugmp]sUOzjge`HB! P8X-o_}4]'G' YHT+(aQQxnJilXSXW .C=vz^`%C]8p3iV3>g\ 9!BqyIsmz$H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3xP!t p7qf j O5TwARMH~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/AEPt>"AEPt>"APFN V:APXV2APrN 2SAPrV1APrX}}2\ARTNASPFASPtBASPtBAUDTB1PtAB1PtABCTSyoBufT L/CMNT 2CTIDLCTNML CTOwLCTTL 2 CpEPDATA@DATA@DATA@DATA@DATA  &DATA DATA DATA 'DATA ?&VCDATA ?&iDATA ?&TDATA ?&ӵDCHTDSamDySN 1Dye#DyeN1DyeN1DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat//GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~wLIMS!!2"LNTD2LsrPaMCHNMODF2CMODL3730NAVGNLNE`NOISROfSc3 ZP1AMfvP1RLbP1WDjVP2AMrP2BAuP2RLn6PBASUPBASSPCON`PCON^PDMFV#PDMFRPLOCZPLOCVPSZE`PTYPL'PXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%RSCAN@SMED SMLtSMPL((USPACA\)SPACRSPACA\)SVER4.0SVER ydSVER,,1VSatd3Scal@Scan@TUBED5Tmpr<UserphARphCHSphDYbigphQLcphTRphTR