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D +,-./0123456789:;<=>?@ABCDEFGHIJKLM +,-./0123456789:;<=>?@ABCDEFGHIJKLMv",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6386ac52c8a84533b1d6ebf07005737dSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well K1320_C9_Hypericum_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z 2Qt+h+5:FgRjQ*\   d x`Pb"2&e+40w6;AGNV^Dgsqu|~ule^ WRNKuIHHIJL_NPRNTWZ]\_awbfb~a`b^W[XTPLHC?:6#1-|)I%F!_;@? 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(@LF&""1F  &&;!>1)!l41W9L"C% >A1L 56iO-9/D:% 4&84  \<  ^$4&` ` !O$ S5}_5"-5b  H OJVi%jGd)Jqy`$s`Oq3/ypR+pp 7'|Ip,8`i"1 KU&J<=38e '%8l?bUmL_8op$HDaKMCc w ?| (6kQ`o Z,`=pi7U "0dc|18/3Y2& <LX&L5>.PWH,,B16 %Uv1!+^-,|+,K8naPp1Gx(X|sp$'Pj*M'~eg9d#!2IK8,ZQIPPLlG26l2i"M/p ep lX-Z9#9Ad:@D"u2$2nnl_JII_ZRk[4H |UVPYX2^>r\FN4wvkA@QVf9Rp#LcEv(#FQ3V'GY~v5Mh(4#kEK8X20sFzZobJ C_3&vg~:tSB'dFS<6'W/  z_wI_OA^|zb"Rx^Czs?A^F"A]`rB 'aPp,`L}?f4h{zx |q]mz.\7l% ,[8yjUY XTGNcktQYMdhmy SD#^@2`CtjS9p_1YE~exmF_s ?E %N MYAAAGGAGGCCCCCCCGGGGGACACACCACATCAACCATGCATAGTCCATTATATACACATGACGGGTATCTTCATGTGGATGGATTCCCTTTAAAACGGGGTAGCAACAAGATGAACGCCATTAGATACCTCTCAGAAGGGCATCCGATGGGTTGGAACGATCCCAATGTTTTATCCGTGCTATCTCCCAGGAACAGAGATCGCGCCTCCCAAAAAGGGCAATGAAGAGGGGGCTGGGGTGGGATGGCGGTCAGGGGAGCGTATATAAGTTGTGTTCCCGGTGTCTATTGCGCTAAGTCAGCTAGTGAGTAGTGACCAGGAGTGTAGAAGGGGCGAGGTGCACCTCCTCATCGTACAACTGATGATATGCCAAGTCATGGTGAGTGTTCCCGATGGGTTCCTAGATTTTTGTTGACATAACGGCTACATGTCTATTTTATAGTTTGTTTCTAATTATTTTTGCAGCAGTAGCTATTACCTGGAAAAAACCTCATGGGGGATGCATTGAAAGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:56:15 -05:00AEPt>AEPt>APFN V+APXV2APrN 2KAPrV1APrX}}2TARTNASPFASPtUASPtUB1PtTB1PtTBCTS{PBufT L'CMNT 2 CTIDKCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA 'DATA ?VV4DATA ?VՊDATA ?VTDATA ?V6DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1zEVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~yLIMS!!2LNTD2LsrPaMCHNMODF2;MODL3730NAVGNLNE`NOISSOfSc2 VP1AMgP1RLcP1WDkP2AMsP2BAwP2RLoPBASUPBASSPCONaPCON_PDMFVPDMFSPLOC[PLOCWPSZE`PTYPLPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SSCAN@SMED SMLtSMPL!!USPACA_KSPACSSPACA_KSVER4.0SVER {ESVER,,1NSatd2Scal@Scan@TUBEC9Tmpr<UserphARphCHSphDYbigphQLcphTRphTR6큅This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN V+APXV2APrN 2KAPrV1APrX}}2TARTNASPFASPtUASPtUB1PtTB1PtTBCTS{PBufT L'CMNT 2 CTIDKCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA 'DATA ?VV4DATA ?VՊDATA ?VTDATA ?V6DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1zEVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat//kGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~yLIMS!!2LNTD2LsrPaMCHNMODF2;MODL3730NAVGNLNE`NOISSOfSc2 VP1AMgP1RLcP1WDkP2AMsP2BAwP2RLoPBASUPBASSPCONaPCON_PDMFVPDMFSPLOC[PLOCWPSZE`PTYPLPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SSCAN@SMED SMLtSMPL!!USPACA_KSPACSSPACA_KSVER4.0SVER {ESVER,,1NSatd2Scal@Scan@TUBEC9Tmpr<UserphARphCHSphDYbigphQLcphTRphTRV"O`x83E> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W;!'*A%pBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfFkY+-^tG|JWoak"T'ogL A(^:gol%`Z9f TA,h{|d-wa$Wx;#2ps'MvnNyag%gcme?{:LKuntikt72Wei@8/(8\_pzN02%B4O7yr[q?Ib\fVLuDq0DkKq;)s;kOgkdd6'Q?@H v\UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4AEPt>APFN V+APXV2APrN 2KAPrV1APrX}}2TARTNASPFASPtUASPtUAUDT~B1PtTB1PtTBCTS{PBufT L'CMNT 2 CTIDKCTNMLCTOwLCTTL 2CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA DATA 'DATA ?VV4DATA ?VՊDATA ?VTDATA ?V6DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1zEVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat//kGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANEKLAST~~yLIMS!!2LNTD2LsrPaMCHNMODF2;MODL3730NAVGNLNE`NOISSOfSc2 VP1AMgP1RLcP1WDkP2AMsP2BAwP2RLoPBASUPBASSPCONaPCON_PDMFVPDMFSPLOC[PLOCWPSZE`PTYPLPXLBRGNmKRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SSCAN@SMED SMLtSMPL!!USPACA_KSPACSSPACA_KSVER4.0SVER {ESVER,,1NSatd2Scal@Scan@TUBEC9Tmpr<UserphARphCHSphDYbigphQLcphTRphTR