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)=51+5-3750(-8 9.)(&"4/C2%5<H=1; $?6<2(4&-0+" $  #  8uU%y(4;-      6789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\     6789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\v",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e808ca27f432400485dd769acf0230ebSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well"K1320_C2_Echinopepon_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z%2F_y5ZEP\=A] / N  L6M?0RYFO R 1 s&cF86<KbLzEZdr#W"s`N;&~{ywsokgdcdeedca``acefhlqy'[%v+^nJ^ o%~_K?6/-8]E d A p0GS2"trmK  "{ed VlVE80--/110-+0>Y>)E \~A[Z uKdEd2OM $6@7?o e%|pha]XTQNKIHGEDB?<841.,,,--,)&$&-=W|:{i56Mrddnx|sX+^lJ3$!#%&%$"     <f4I]U/KoC%        FVhIxv.   !Ct0]rlK\0  $LO{X[1   'S+nLd/ &P3IAk9     25wKC             = sJ,DP9q,~P B\Az3     D^. 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"$%'(*+,-/024566778:;=?@BBBA@?>?@ABBA?=:9988764310//.,*(&$!  5Zx{^9  "####""#%(+/2579<>AEJQX`is| /?N\it*7BJQUXY[_fo{0EYiv|o]G-=%|bX2In m.skfca`^\[YXWWVUUUTSPMJGDCCDEEEEDDDDDEEDDDDDEFFGHIJMRYbo#@]uwY0  +U qb\Dr+C ' cZ-p5[})lJ(  4Y,l$z!P~!P{oe[SLHGKWl@IOW>pdpgb_^]\\[ZYXVTQNKGD@=:87641.,**-4?NdJt8gRQe"HO;vR:,')3Hh$|1zzihug&y]?11=V|CJlVRa  \/Z=.(%$" 1S8 fW+)Lkub,yq>  0l3 xdU!   +b lHK        %m^Mx19\!%)++(#!%)++)%          [h j;E   \J4`Fqy+    -}y>Ez38aZ=  hfL0&9Rh 0 Ww'XI #&#   .fi!5          ?6#Xm%8'J*~[I4 .    +`a 1   6 9.hs&   gP h}-PB3\;R[  i]#.5   SA6r,7E    "uky7%:     /u4QLNl&      Lb;     \U7Q!.G  Jo=   -wDfG  *tHU      6ZU       I]{*m*    KY3   <g/B=!U0    5LvZ    *253.&  S8 "I-J^cXC,,7<;4(    MFX22  ^ 3 &#AB[AXAN֡AJKB.bcpKB_3730_POP7_BDTv3.mobtermGAAGTGACGCTTGTAAACATTGTGGTCTTGGCTCTCCGCCCCCCTACCTTATAACTAACTATGTAGAAGTAGAAATTAGAAAAAAAACTTATGTAAAATTTTTTCATAATAAAGGAGCAATACCAATCCTCTTGAGAAAACAAGAAATTGGTTATTGCTCCTTTACTTTCAAGAACTCATATACATTAAGACCGAAATCTTATCCATTGATAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACCAAGAAGTGACGCTTGTAAACATTGTGGTCTTGGCTCTCCGCCCCCCTACCTTATAACTAACTATGTAGAAGTAGAAATTAGAAAAAAAACTTATGTAAAATTTTTTCATAATAAAGGAGCAATACCAATCCTCTTGAGAAAACAAGAAATTGGTTATTGCTCCTTTACTTTCAAGAACTCATATACATTAAGACCGAAATCTTATCCATTGATAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAGTTGTGAGCATTACGTTCATGCATAACCAA7Qdq ",5DLT`r'.7AJXajt +6CMXcmx"-:EO\hr}  +6CO[ep{ $0:FPZdmw +7BMYep{(3>HS^ju    ' 2 < G S ^ k t     # . : E P [ g q |   , 6 B N X b m w   # . 9 B7Qdq ",5DLT`r'.7AJXajt +6CMXcmx"-:EO\hr}  +6CO[ep{ $0:FPZdmw +7BMYep{(3>HS^ju    ' 2 < G S ^ k t     # . : E P [ g q |   , 6 B N X b m w   # . 9 B     >8>%%% 6>+3"">;++>;11>>;;>;>;>;>;>';>;>;>>>>>>>;>6>;>>>>>>;>>>>>11>>>>>>>>>;>>>>>>>;>>>>116>>>;;;>>>>>>>>6>;>>;>>>;>>>>>>>>>>;>>>;>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>;>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;;>;>;'0$      >8>%%% 6>+3"">;++>;11>>;;>;>;>;>;>';>;>;>>>>>>>;>6>;>>>>>>;>>>>>11>>>>>>>>>;>>>>>>>;>>>>116>>>;;;>>>>>>>>6>;>>;>>>;>>>>>>>>>>;>>>;>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>;>>>;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>;;>;>;'0$ \>,!6<WP\X'N15<('$T&G7RG8LZ4!=&R2ELJ#5G-* >X UK@[4<D#1b,F3@4T3?FX!;Z [M(U!N8' O@5!<$"XLZ ;)AMUB7@42?L8&&;MLD-D V= L2%D.1B='`IS,:MI_YSb"2<4W )%-% VLY!XFc0C &"GK3 F$X; ]!(E,6FZ>8-&JD*a">K\BBM7]=6^K:)_;.U6  Tn8;n@M&,pb % s -b8m& R^7,Y^A`GNfp6'h|LulbLyRonS^OgW% D*T`6SLW(% G=VF*F3(  "mnBRTfH]Q}?j 74[0zAs?Jn "eg{. %BAv+G\@Ucsz[PcT5@OR+8;716OI$1&2$ Q9 k GLpp,s,o(o2"#E8|MdPd8:S*^T*dvspdp84J75J2p+,I\D Hp6KxepzEw05'?OwIC%0$v= \* gMmDUPy, ++C2 .g/3b%'!/E':%  TZ672%! $ ]P, $&%5:0 8,  !  #!6% ##$-"  %,5,3".3 :)M$&*@DBKMc">uDAGGAGCGGCGCGAGGGGACCAAAATCACGTCGAACTGCGAAGTTACATGATATCAATCAATAAATAGGAATAGTTAATTGGGGTGCCACCCATATGGTACCCGAACATTATGGAAGAGCCACAACTCTTCTCAAGAGTGCACGAGGTAGCTGATAGCGCTTCAACTCCCACGAGCACTCATATACAATGAGAGCGTTATCATATTTCCCCCTAGAAAGCACCTCCACCATCTCCCCCTACAGAAAGGAGGTGAGAATAATAAGCACAATGCCTAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:36 -05:00AEPt@AEPt@APFN V=APXV2APrN 2[APrV1APrX}}2dARTNASPFASPtpASPtpB1PtoB1PtoBCTS9BufT L7CMNT 2CTIDL CTNMLCTOwL'CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  .DATA DATA DATA 'DATA /_VFDATA /_.DATA /_DATA /_uDCHTDSamDySN 1Dye#DyeN2DyeN2DyeN2 DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!2*LNTD2LsrPaMCHNMODF2KMODL3730NAVGNLNE`NOISOfSc4 ^P1AM*P1RL*P1WD*P2AM*qP2BAP2RL*GPBAS6PBAS!PCONݴPCONܟPDMFV&PDMFPLOC*uPLOC*KPSZE`PTYPL/PXLBRGNmLRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SCAN@SMED SMLtSMPL##VSPACMSPACSPACMSVER4.0SVER .SVER,,1^Satd4Scal@Scan@TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR"This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN V=APXV2APrN 2[APrV1APrX}}2dARTNASPFASPtpASPtpB1PtoB1PtoBCTS9BufT L7CMNT 2CTIDL CTNMLCTOwL'CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  .DATA DATA DATA 'DATA /_VFDATA /_.DATA /_DATA /_uDCHTDSamDySN 1Dye#DyeN2DyeN2DyeN2 DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat//TGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!2*LNTD2LsrPaMCHNMODF2KMODL3730NAVGNLNE`NOISOfSc4 ^P1AM*P1RL*P1WD*P2AM*qP2BAP2RL*GPBAS6PBAS!PCONݴPCONܟPDMFV&PDMFPLOC*uPLOC*KPSZE`PTYPL/PXLBRGNmLRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SCAN@SMED SMLtSMPL##VSPACMSPACSPACMSVER4.0SVER .SVER,,1^Satd4Scal@Scan@TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR'%N`!2Fu4WyYZ(Wa:nuXK|+U17ujZj_!p-">R2`Yyb oJH.`Ep2=TgVSQ<}LgU!IZHp#)+KT[^[G^%E:"*l#qN8>o Ueh,9n5NJw=;SocJyw*6TdO{7I{4^PrtC[J19D5mr+8B9.~;{lUz{19se0:=y=&e=1&!rEV(V-IAG&e"i/ud?7OBmxCB#v6|k.j H_H-CI;{dY7cI*]5cP}Gs+OStxHlYxC?(cU/~!-,'bF4$T9tR3#=cC0^,!Sk2%vAfIx+C!K4#]BN $`?"*JIf7Qv6,MZ@Y+P'#9r??8r]f.iD4ZI,|Q|T{\K71P3;fTR[fRr;-6U@;EGCfQ&HQ$ 0603XXbLmX;9 |.W162Q$OEHO6z7B-_8Es{sMV^@}Ouxg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2s JX0bt-] 5y$47y 4orKOR`{A,}@1%i`+|*I_Bi4}V[Dj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0V8JX|K2-!;9xxsWD@|DmOY,f *":4L\\W'KFxz2jA&owbBV*d>AEPt@AEPt@APFN V=APXV2APrN 2[APrV1APrX}}2dARTNASPFASPtpASPtpAUDT gB1PtoB1PtoBCTS9BufT L7CMNT 2CTIDL CTNMLCTOwL'CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  .DATA DATA DATA 'DATA /_VFDATA /_.DATA /_DATA /_uDCHTDSamDySN 1Dye#DyeN2DyeN2DyeN2 DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabFVocFWO_GATCFeat//TGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~LIMS!!2*LNTD2LsrPaMCHNMODF2KMODL3730NAVGNLNE`NOISOfSc4 ^P1AM*P1RL*P1WD*P2AM*qP2BAP2RL*GPBAS6PBAS!PCONݴPCONܟPDMFV&PDMFPLOC*uPLOC*KPSZE`PTYPL/PXLBRGNmLRMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SCAN@SMED SMLtSMPL##VSPACMSPACSPACMSVER4.0SVER .SVER,,1^Satd4Scal@Scan@TUBEC2Tmpr<UserphARphCHphDYbigphQLcphTRphTR