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***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 0158277543a64fefb8d4e47910c0a022Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well/K1320_C1_Marattia_weinmanniifolia_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7zCez*a 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(Dj}X:&   !&)("+O~ |R5&$,9ENPI<+   !%#        "%&&%# !$%$  $$                          !+-'                                                                                                           A}A!?AQ_AkQKB.bcpKB_3730_POP7_BDTv3.mobtermAAAGTGGTACTTTGTCACATTTTTGGCTGGCTACGTCCGCCTTCTTTTTTCCAGCCATGGGGAGATACAAAGAAGAATCCTTCAATTCTTGAAATTATTTTTTCAATCGATAGAAACTTCTTTTTCAAGTAAATACGTAACGAAATTTTTAGTTCTATCAAAAGATGAAACGAAAGATGAAAAATCAAACAAAATTTTAAGAATTAGTAAATATTAACTAGAACTTTTTTTTTTTTTATTCCAGAACCTAGTTGAAAGGTTTCCGAGCCTTTCAACTAAGTTCTGAAATATTTGCGTGGTCAAAAATCATTGCGAATAGCATTGATTTTTTGACCAAAGTTTTTAATGAAACAAGTTTCTATTGGTTTTGTTTTTTTTACGCATTTACGGAAGGAGCTTCAACAGAAGCTAAATCTAGAGGGAAGTTGTGAGCATTACGTTCATGCAGTAATTTTAAAAGTGGTACTTTGTCACATTTTTGGCTGGCTACGTCCGCCTTCTTTTTTCCAGCCATGGGGAGATACAAAGAAGAATCCTTCAATTCTTGAAATTATTTTTTCAATCGATAGAAACTTCTTTTTCAAGTAAATACGTAACGAAATTTTTAGTTCTATCAAAAGATGAAACGAAAGATGAAAAATCAAACAAAATTTTAAGAATTAGTAAATATTAACTAGAACTTTTTTTTTTTTTATTCCAGAACCTAGTTGAAAGGTTTCCGAGCCTTTCAACTAAGTTCTGAAATATTTGCGTGGTCAAAAATCATTGCGAATAGCATTGATTTTTTGACCAAAGTTTTTAATGAAACAAGTTTCTATTGGTTTTGTTTTTTTTACGCATTTACGGAAGGAGCTTCAACAGAAGCTAAATCTAGAGGGAAGTTGTGAGCATTACGTTCATGCAGTAATTTTA7Uaf} ,<P_k} +5COXdp{.6DLV^hs *7BO[gt~'4ALWbp{)4?JU`kx+8DO\fr}!-9DQ[gt  , 8 D O [ g s   ! - : D Q _ j u    % 1 = I R _ l w    ) 5 @ L X e p }  " . : F S ^ j t '3?JVamy $.;GS_kx(5BMZep|&2?JVbnz -:FQ]ku*6AMZfq)6BMZep} )4>7Uaf} ,<P_k} +5COXdp{.6DLV^hs *7BO[gt~'4ALWbp{)4?JU`kx+8DO\fr}!-9DQ[gt  , 8 D O [ g s   ! - : D Q _ j u    % 1 = I R _ l w    ) 5 @ L X e p }  " . : F S ^ j t '3?JVamy $.;GS_kx(5BMZep|&2?JVbnz -:FQ]ku*6AMZfq)6BMZep} )4>!* "#*/>>>>>>"+ >>% */>>>++;>>;;>,;;'>>>>;3>>>>;>>;>>>;>;>>>>>;3;>>>;>>;;>>;>>>>>>>>;1>>;>>>>>;>>>>>>>>>>>>>6>>>>>;>>>>>>>>;;>;>>>>>>>;;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>8;;6++6>>>>>;;>>>>>>>>>>>>>>;;;;;821'621;8;1;;;>;88>+2133$6.283;;3++..3132833;>;88;;>8;))";23>;>>>>;;8;8;1';;+8"+;;8>;;;>>>>>>;8;;;;;>>>8;83>;;88>>>>;88+332>28;;;>;;>>>>>383"3+3>>>>8>>83>,338883&23 883>8>2&&2%'88>>8838&333388>>>>&883*3  !* "#*/>>>>>>"+ >>% */>>>++;>>;;>,;;'>>>>;3>>>>;>>;>>>;>;>>>>>;3;>>>;>>;;>>;>>>>>>>>;1>>;>>>>>;>>>>>>>>>>>>>6>>>>>;>>>>>>>>;;>;>>>>>>>;;;>>>>>>>>>>>>>>>>>>>>>>>>>>>>8;;6++6>>>>>;;>>>>>>>>>>>>>>;;;;;821'621;8;1;;;>;88>+2133$6.283;;3++..3132833;>;88;;>8;))";23>;>>>>;;8;8;1';;+8"+;;8>;;;>>>>>>;8;;;;;>>>8;83>;;88>>>>;88+332>28;;;>;;>>>>>383"3+3>>>>8>>83>,338883&23 883>8>2&&2%'88>>8838&333388>>>>&883*3  \>:ECC))+<4 '/S 1;.]!%).D4M1)!B0I;;?R0 N3.63;J4($D=:Q( B1'3/EWL(UL"E2d_SF=-Nb\?FP4`EHZ2*$*U_RGK<'+?/`-KMO\DR4AYYX. 'L)W0@3B?G] <2E]<' 77CFM+NL,6>3 "%>Z< R-c!)Z/Ab Rc]-\<LDFZ%=3*a>"^A,5C-V [L.+(;Mb &K 8RF;DZ1U_;\:/04._ 3R01%J$5[@JR+$FVO= Gb/!B1: ;  5C#TEE7XZ!4>^F<^ !,LT7Na:>NFU_\O3RcHH:DF KR9bB <C*F Q 3<W-M 1M+Kd? P_$ S51,%@3\,[, <`E2cN`DAT 5/$ Q6  m ^Da6n\ *<mn,)e3k,F7Cm [N3mO 5cxQD#dlc2 F.uPqo$Q3oe5b?2~x6hDi"j3o}XE0nFB3y xAQP&V)ej-cptm4l9U~,?/ hH$ :] (a1Mk(QY %W>. =[9K%|YFAGx D(\i*C3Xy +' u/ [w5[I 8~cKG ' 4twpzA<%^A`-U  J@]@:, H-s\  QahDI:=oDMu]wHTLT;A8 4%,3>!& +H6S # >' %- '       !             $:, /  1/""1     K3=P-gL, p*)OT7r-lpv, >s wTA F()B{$T8*udd]EJdE>`#N'Mp4&[H!Es_*= x:p~} #>QZzZSu*6o, ~88T`#Wd+/Ud,88Hp|YPD})hPRps8?Td^"2Eww rd\tV,@-b@ 5pGwL FrT i-.:` {$">DA 5TYos,mcT\q` <#z5  q4/1 8 Ps 78'j% ZRG*5B78C+ "%1 2!8# -AJ>8%-'"+  J6$   " !;]oa."9@*"*   EFss4M=JI`wAR;hsQA:-TYH1pVf\i^4&N.[[bv8xjVhEP-g1R% 3Z\i?Y3-5c:<5~cc ors9[ fCN Q@CdCI=I3BfYU4En|( ]iC,V,0W8JARd~uur_{J>I@,WPhBAXp;eU_`Gn3QZerSQcju.}_GGGAGTCCCGCCGCGAGGGGACAAACTGTCTACACCTGCGTGCTCCACCCTACAGACGTATAGATACACCGAGGAGTATGCAACCACTCCCCTTAATAAAGCCACCCGATAGAGGGACCTCGACCACGCCGCCCTGAGGGACGGGAAACGGTGCTCTGTCCCGATACGTTGCGGGAGGCGTTTTCTCTCAGGCTAGGATTAGTACCCCTCCCCAAGGGGGAGTTCCCCGCAGCCAGATACTACAGCATCTTGGAGGGATGGCTGAGAGTCCCTCCACTGTGCTGAGGTATAAGTGCGTTGTCCGTTCTTAGCGAGTAGAATAGAGAAACGGAGAACGGAGGCAATTGAGGCACGAGGCTCTAGTTGAGGTGGGACCCACACGCACACGCAGGAAGATCCTCCACAGGCTCTTTCTCTAGAAAGGAGGTGAGAGCAATACGCTCGCGTTATCAAAATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:35 -05:00AEPt>/AEPt>/APFN VjAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPt\ASPt\B1Pt[B1Pt[BCTSqBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA >}VsDATA >}1DATA >}QDATA >}ϭDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~o~LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISMsOfSc8 nP1AM_vP1RL[P1WDcP2AMj&P2BAmP2RLfPBASOnPBASMPCONZPCONXVPDMFVSPDMFMPLOCTPLOCQ6PSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%MkSCAN@SMED SMLtSMPL00V#SPACAcSPACMSPACAcSVER4.0SVER pSVER,,1~Satd8Scal@Scan@TUBEC1Tmpr<UserphARphCHMphDYbigphQLcphTRphTR#This is the confidence range *ABI_Limits..FVocAEPt>/AEPt>/APFN VjAPXV2APrN 2{APrV1APrX}}2ARTNASPFASPt\ASPt\B1Pt[B1Pt[BCTSqBufT LWCMNT 2=CTIDL-CTNML3CTOwLGCTTL 24CpEPDATA@DATA@DATA@DATA@DATA  NDATA DATA DATA 'DATA >}VsDATA >}1DATA >}QDATA >}ϭDCHTDSamDySN 2Dye#DyeN2 DyeN2%DyeN2*DyeN2/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabbFVocQFWO_GATCFeat//"GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~o~LIMS!!2JLNTD2LsrPaMCHNMODF2kMODL3730NAVGNLNE`NOISMsOfSc8 nP1AM_vP1RL[P1WDcP2AMj&P2BAmP2RLfPBASOnPBASMPCONZPCONXVPDMFVSPDMFMPLOCTPLOCQ6PSZE`PTYPLOPXLBRGNmL%RMXV1RMdNDRMdV1RMdXDRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""LS/N%MkSCAN@SMED SMLtSMPL00V#SPACAcSPACMSPACAcSVER4.0SVER pSVER,,1~Satd8Scal@Scan@TUBEC1Tmpr<UserphARphCHMphDYbigphQLcphTRphTR"3Z2VW)$>ZHm? 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