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A !"#$%&'()*+,-./0123456789:;<=>?@ABC !"#$%&'()*+,-./0123456789:;<=>?@ABCv",,III^^^{{{&&&999DDDXXXaakvv      ***33>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ]UUUMMMmlm      ***444===FFFOOOXXX``fhhpppxxx~~~<<<<<<<;;;;<<<<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b20b82bdd7ac44b2bd00387bd3c6796eSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-27_16-08_16593130Seq18016137733_K1320_trnh5fjmadera96-Well K1320_B6_Desmodium_trnh5f_trnh5fKB_3730_POP7_BDTv3.mobSeqPop7z-5Lm "#&(I*J,6.M0376;O?E$K QYbWkv|;tBl.-)*70;F0M.PpQ3Q>QSV%XZ[['ZZXV`SP KFA<&61-Q)9%"k~'8unvZ N h $ a [  ( g _ m lC`]Gz^   'Ayem.28  hT1g}}&] %(&   {/ ,09^j?GNl~pJ   /T`(.IZ]TE838CKI;& !HojC   3{^A)@=O+ &=H?")LU:"y+C| A e ?)'<T'* 4sqfM+ `[ m4O   (+%   0H{-m&3Usx\5 (ASUG.&13+)Fd}y]KNiuoRo2'ZhO()GmyS) ,J^bU< (DY_S: DxvB  &Km~uT& DpRN^nlQ&$u.[QQ ij3R<T(5g^ %rnGF|\=W!D4bd/q'`f/ $+-2?TiskS3 7z> &-CmaBa$[S!&F[^P7286L~E@l{\/ 0NXK- !#" !++ .62% 5%VHZ;KBpypuD&  .sd{/J MqE lL:*    &6CNV]ce`P76KQF/IwqF%*Ktzjgo|w|AdeC  /0&hGFb~}pR, 0dm=%=PVK5Cyx?>^iZ; 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" "."$"&(""+"$+))"+#"0"+13;266("$"+'"   0;1+%"++2..%  "2".!-,!;&".'.++22' 122"2'#  )"'))."&&,+;3  3   ,                         +8'+ ++ "         &" ""&.!   " "."$"&(""+"$+))"+#"0"+13;266("$"+'"   0;1+%"++2..%  "2".!-,!;&".'.++22' 122"2'#  )"'))."&&,+;3  3   ,                  ZDS`Z+.A:"@U09CWE>7:6`SJ %<"&3-!7VD )2 8_GQ6K+dR%YG\6P`_RBWbN0-(S>HD,^2I6B.R4!5U^\T^ZNM:23 SWb_6`[\!U2K`7 4SZ$ ;1HP!&A"8IU_Fb/% -*H=" >SR). DHCFQ;I5[?_#J]$N#3*3.-Z:4;B'! #" A,!:X(TISLL]a W!!_1FO61`-L'78A/-/)P&2_$caV V417!:Y1A8"F.+c2FEL<PdPPS92 LbF2I\( 8*2RdV)0?.-5<d:D?]G I'EE ^JV:.EJ'3%C)+BEUAC.2^QE .D8#)&= GB4#)E-aEbC'_T&9 a^ MOuGV 9ah,q8.m5m:{w_ (*:m( !|Zb-]E  xy"3O]aE(bQQj,a\UzmD6lGJHv[^E)/~8*p[VJZFuOS t9KuV2 NC(1"99z^x a@ Hl Z+"{%D9wQ&Eh\i9 a]" aSJ\^__*| 7 f KLE(@   >: Zf ! 1 `R  F ik_#zsq*,g'A&c[rX-Sh=( 0*Wb p( jqHs^tv)l/lu85!bylHYIOy*L #_68s(YgiM3/s p54w1  "$./Hk x#  [ 5eV+/8! =$; ?>,%# + $:/E  +)%7|;q /dK2%J/ HK0 O#*,9)') KK KKKCKKK  KKK KKKKKKK K  K   K  KK  KKKK\6pn\JEt2 , ^A'fa{c(#8w~POKu$iS\G x`$n 178I)dK1_EZ;7s#0,d GQz84dU,zC,Lm,Jds ]s5>.T,6,8,p#8&+f\q31yx8;,\L {3:  u88,8Jp8?sd$ZlUl3 88dn{7{,d?H`%F&F 2fFS5 J{A7hDE1m\ohvB(u.-?cMT:{*,m &OtDMOV,C6>5/nN u8V@-kB;2eC`{`dCddRiLvJ<Kl/# !zRObp?TdX wOhzc VhY{2htupQ,Kk"=z7^!2nAdk,{tilC  dqL"v5/7=%T~< .7 .l5Gf%6(J&G&3UjiYxkJ\ 3%+ Ah-aV%q'AAGGAGCGCGCCCGGAGGGAAAAACCCATTTGGTATGCATGGCTAAATGAAGAAAGATAAATATAACGAGAGCGATGGGGGAACCTCCCTAGAGATTGGGGGTGAGGAGTTGGGGGGGTGCGAGAGACCTTTAGAGAGGGGCCGTCGGGGGGGAGCAAGGGCGTGGGCTCTCTGGTGGTGGGAGGGGGTGGGGGTCGGGGGGGGACCTCCCAGAAGGGTAGCTGTGTCGGGAGGGGGGCTGGGAGCGGGAGGGATGAGGGAAAAAACGAGTGACAATGCTTGAAAAGGTAAGGGTCGCCGGGGAGGGGAAGGCCCCCGTTGAAGAAAAGAGGGAGGAGAAAGCGCGGGGGAGGCGAGGCAGTGCTCGGCGTAGATGAATAGCACGAATGTATGTTTGCGGAAAGACTCCTGCGAGGGAGGGAGGCGAACTATTATGAGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-27 17:55:53 -05:00AEPt>AEPt>APFN VCAPXV2APrN 2cAPrV1APrX}}2lARTNASPFASPtVASPtVB1PtUB1PtUBCTSvBufT L?CMNT 2%CTIDLCTNMLCTOwL/CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  6DATA DATA DATA 'DATA ?TVLDATA ?TՠDATA ?TTDATA ?THDCHTDSamDySN 1Dye#DyeN2DyeN2 DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FWO_GATCGTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~tLIMS!!22LNTD2LsrPaMCHNMODF2SMODL3730NAVGNLNE`NOISSOfSc5 bP1AMpeP1RLpaP1WDphwP2AMpoWP2BArP2RLpkPBASUPBASSPCON_PCON^'PDMFV,PDMFSPLOCpZPLOCpWGPSZE`PTYPL7PXLBRGNmL RMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SSCAN@SMED SMLtSMPL!!V SPACA_SPACSSPACA_SVER4.0SVER uSVER,,1fSatd5Scal@Scan@TUBEB6Tmpr<UserphARphCHSphDYbigphQLcphTRphTRHThis is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN VCAPXV2APrN 2cAPrV1APrX}}2lARTNASPFASPtVASPtVB1PtUB1PtUBCTSvBufT L?CMNT 2%CTIDLCTNMLCTOwL/CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  6DATA DATA DATA 'DATA ?TVLDATA ?TՠDATA ?TTDATA ?THDCHTDSamDySN 1Dye#DyeN2DyeN2 DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabcFVocRFWO_GATCFeat//#GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~tLIMS!!22LNTD2LsrPaMCHNMODF2SMODL3730NAVGNLNE`NOISSOfSc5 bP1AMpeP1RLpaP1WDphwP2AMpoWP2BArP2RLpkPBASUPBASSPCON_PCON^'PDMFV,PDMFSPLOCpZPLOCpWGPSZE`PTYPL7PXLBRGNmL RMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SSCAN@SMED SMLtSMPL!!V SPACA_SPACSSPACA_SVER4.0SVER uSVER,,1fSatd5Scal@Scan@TUBEB6Tmpr<UserphARphCHSphDYbigphQLcphTRphTRPy]]~I8e0MSayQckycji[&`&h2hA:DCDFIt~FRi^aL7(p~XVG ;W/0'o ocDGRFT16Y-e+&5%`7lZ!O)>C9iu2Zv&1x5roz;P]pDuYLDK2NB&_SF`|z&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/YgnCCHY<6(mu y-WLa)&:\.~x|MI>"J'8O[,_a2'Kdb"U"=C!(3by{,ty\bH?6g%3tk?$[WturW>4GPQEdrB-8'i2{ysPl`A?^NHEDX+9m#k:X=<;`O|g+4b|s:hlt$B!(:O3dT:HT,} IlE]&l8EE.Om,\eyDWYI!8U-o9D4lxXW[uTs|$@^]?\Hif3qE`HoG83:8\_pf|r,m &$;u<=p{83T:U~`}'vVE$;;OwV-Op!zBwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{@2K/^1/|+x"i40}jf}pdZ8G72W3Un^=M\~[!iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/AEPt>AEPt>APFN VCAPXV2APrN 2cAPrV1APrX}}2lARTNASPFASPtVASPtVAUDT6B1PtUB1PtUBCTSvBufT L?CMNT 2%CTIDLCTNMLCTOwL/CTTL 2CpEPDATA@DATA@DATA@DATA@DATA  6DATA DATA DATA 'DATA ?TVLDATA ?TՠDATA ?TTDATA ?THDCHTDSamDySN 1Dye#DyeN2DyeN2 DyeN2DyeN2DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1EVNT1FTabcFVocRFWO_GATCFeat//#GTyp!!1HCFGCEHCFGHCFGHCFG==InScInVtLANE.LAST~~tLIMS!!22LNTD2LsrPaMCHNMODF2SMODL3730NAVGNLNE`NOISSOfSc5 bP1AMpeP1RLpaP1WDphwP2AMpoWP2BArP2RLpkPBASUPBASSPCON_PCON^'PDMFV,PDMFSPLOCpZPLOCpWGPSZE`PTYPL7PXLBRGNmL RMXV1RMdNCRMdV1RMdXCRPrNKRPrV1RUND RUND RUND RUND RUNT /RUNT 7RUNT RUNT 6Rate 1RunN""KS/N%SSCAN@SMED SMLtSMPL!!V SPACA_SPACSSPACA_SVER4.0SVER uSVER,,1fSatd5Scal@Scan@TUBEB6Tmpr<UserphARphCHSphDYbigphQLcphTRphTR