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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR SSSMMMlll   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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ef2d03c971464679a144d625632f3bbaSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_15-12_16543130Seq18009137720_K1319_trnhjmadera96-WellK1319_E6_Salvia_trnh_trnhKB_3730_POP7_BDTv3.mobSeqPop7z1b{xz; 7 h *. 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(-* "'$ 1=A</ &3<A?8- " )5<:0!  #$    $.1+   ,596-!  0AHD5 #.31)      $-0+ )11)  $.5761+#     <mAJA"9XAV?AKB.bcpKB_3730_POP7_BDTv3.mobtermATAGTAAAGTTTTAATTTTTTACTTGCTCTCCGCCCCTCTACTATAAATTAGATTTATAATTTTTATATTTATTTACTTTTTCCCTTTATGAATTCAATTTTTCAATTTAAATTTTTTTAGAAATTGTAAAACAAAAGATAGAATTTCAATCATTTTTTTTTTATTTTCTTTCTTTATTCTCGAATAGAAAGAATAAAGTACAATATTTCTAGAAAATATGGAAAAGGACTCCTAAATTGAAAAGAAAATAAAGGAGCAATAAACCCTCTTTTGATAGAACAAGTAAAGGGTTTATTGCTCCTTATGTTTATCTATTCTATTTTCAAAAACTCCTACATACTAAAAACAAATCTTAGCCGTTTGTTGGAGCTTCGATAGCAGCTAGGTCTAGAGGGAAGTTATGAGGATTGCGTTCACGCATAACATAGTAAAGTTTTAATTTTTTACTTGCTCTCCGCCCCTCTACTATAAATTAGATTTATAATTTTTATATTTATTTACTTTTTCCCTTTATGAATTCAATTTTTCAATTTAAATTTTTTTAGAAATTGTAAAACAAAAGATAGAATTTCAATCATTTTTTTTTTATTTTCTTTCTTTATTCTCGAATAGAAAGAATAAAGTACAATATTTCTAGAAAATATGGAAAAGGACTCCTAAATTGAAAAGAAAATAAAGGAGCAATAAACCCTCTTTTGATAGAACAAGTAAAGGGTTTATTGCTCCTTATGTTTATCTATTCTATTTTCAAAAACTCCTACATACTAAAAACAAATCTTAGCCGTTTGTTGGAGCTTCGATAGCAGCTAGGTCTAGAGGGAAGTTATGAGGATTGCGTTCACGCATAAC #>Yiw&9I^%2<FP_hv~ $-8DNZgt $1?LY_jqz,9FQYdr~'2>IUamz +6CP[eq~(5ANYfr~    ' 1 < K W b n {    , 9 E R ] i v    * 4 @ L Y f q |  + 7 C O [ h t     ( 3 @ L V b m z ,7BO[gt&2?JWbnz !-8DO[it*5AN[fr(3?LXep{'3=JUdn| $0<HT #>Yiw&9I^%2<FP_hv~ $-8DNZgt $1?LY_jqz,9FQYdr~'2>IUamz +6CP[eq~(5ANYfr~    ' 1 < K W b n {    , 9 E R ] i v    * 4 @ L Y f q |  + 7 C O [ h t     ( 3 @ L V b m z ,7BO[gt&2?JWbnz !-8DO[it*5AN[fr(3?LXep{'3=JUdn| $0<HT   :    +!4/>+8/)>>>>'//>>,'2),;3    ";>;>8''>>>66>>66>6>>;6"  ';;(+((';;>>>;>>>>;>>>>>>>>>>>>>>1;>>>>>>>>>>>66>>8386>$6>8;>>>>662%""+""+81>>>>>>>>>>>>>>>8>;.6;>;>>;>>>>>;>8>>>>>>>>>>>>>1>>>>;;;66>>>>>>666(8&.33;>;>>>>>>>;>;>>>>8;2.812&&++83.+20"+3332281;.8008208;>0060.;;>66>>>68;0+6+>>22822+>11>"'#     "     :    +!4/>+8/)>>>>'//>>,'2),;3    ";>;>8''>>>66>>66>6>>;6"  ';;(+((';;>>>;>>>>;>>>>>>>>>>>>>>1;>>>>>>>>>>>66>>8386>$6>8;>>>>662%""+""+81>>>>>>>>>>>>>>>8>;.6;>;>>;>>>>>;>8>>>>>>>>>>>>>1>>>>;;;66>>>>>>666(8&.33;>;>>>>>>>;>;>>>>8;2.812&&++83.+20"+3332281;.8008208;>0060.;;>66>>>68;0+6+>>22822+>11>"'#     "  2J<I$D& 4L.WO<)JcO=/ 8:2A@b9],>/ROR;.I6S:I2$A0,_>]V '9L]#@+#>PDKG9D:! :IMW!?@ZEZ E .Q/_57P2b/7/LKZTRT6@Uac$ CU&`_DT;T.'HDO-3W`"FC+-=G,1%\0 .^--[Y,0/:4DL^<cHU46&ND48SN'ER\KX QLc5U%MF'0S( K N3?!;)\R`b. 4 CE^%-dJa7BIQ[L7 b 0"]W%M# ??*%LC>F@5)O' bG G=33H[a!J V-XC34RPAV%La]<J& 3I\OW T<'3[E#([N-@',$<]XM*X,9AU:)P3QL: %G(@9TL0%dU2n3]4<$ /fjtz=2FsAiKDpif xy< hv[PL4dpE'(M<tn"N{V%onIZCqP CI~x )gV Lr)o=l}l(UY6X%t6}tK8\{ys}fna]^li 4Lv1.S()S.S+jwJ?TJ%B L j<F;`O6vyLs_yT :0X;-4vENhquj #EiR)  Wn6=CJP (Us!r3bh@yK ]#H.7Mu@UP.dA$<?"ME  )# *3 +PZ5 ~H9@ ) J&&   3` % 1  2(k+^!@"/  $    %H0*:22?S?f -A! V%I 0pupS~p<VpXx} n|pe>lS0oO!P.axd18fU4uI6JR_>c:Ad)<?<qk0| J| X6l_|,]9'dHhQ$7 8}Et "uI%=J 8A$@575m1a8sO?D9\H a"U18lZrK2z"6 2 BDK= j!I\P#g'zCaL*93$)DN<!'GE6n9G=_Ae8GY>,)!4'G?HPU+."+)fA/$5F$(M.F889A2O6G;5>=*8<7"&2#T+A)+1GkjKgmN7,' 9)+418<T1_R;/a/%X+1Z(6#.A''<.D*G.@H8"4/ _vB%>,>ESO528.*N4$@:'E.O'>XgbS3Pr(Gf[5AS0Nr/%IRgTZDe0=5Z2+6F`QG;$GID|:GBdA:XNE@!,*1N];V@?s/@ :80jt M0f^y/,[41GAGAGGGCCCCCGGGGGCCACGAAGTTAAATGCGAGGGTATAATATTTAATATAGATACGGGCCATATACATGGGGGCCCCCTTACAAGAAGGGGACTAAAAAACGAGAGGTGGCCAGCCTGCCCCCCCGGGAGGCCCCCTAGTGAGGCCCACCCGCCGGAAAGAAAAACGAAAAAGACACGCTTATCCTCCTGAGTGTGCTCCCCAAAACGATTCTAGGTAGGTCAACACTTCTCTAGTGGTTACTGTGCGGAATTGGTATGTTGTCTCACCTTATAGTTCCAACGTACAGAAGAACGAAAAATGAAAATATCTAATCTAGGAGGGGGGACGTCCATATTATTTTGACCTATAGTTTTCCAATCAAAGAGAGGTGCGAATAGAGCTAGGTCGAAAGGAGGGGTTTCCGCATTACGTTAATGCGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 16:59:53 -05:00AEPt<AEPt<APFN UrAPXV2APrN 1APrV1APrX}}1ARTNASPFASPt8ASPt8B1Pt7B1Pt7BCTSnBufT KmCMNT 1UCTIDKECTNMKKCTOwK]CTTL 1LCpEPDATA@DATA@DATA@DATA@DATA  FDATA DATA DATA &DATA ?~U{DATA ?~{DATA ?~Q{DATA ?~{DCHTDSamDySN 1-Dye#DyeN18DyeN1=DyeN1BDyeN1GDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FWO_GATCGTyp!!1 HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mALIMS!!1bLNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISMOfSc\P1AMR^PP1RLRZP1WDRaP2AMRhFP2BAkP2RLRdPBASO_PBASMPCONYUPCONWPDMFU[PDMFMPLOCRTZPLOCRQPSZE`PTYPKePXLBRGNmK=RMXV1RMdNCRMdV1RMdXC)RPrNK RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1RunN""KS/N%M{SCAN@SMED SMLtSMPLUASPACAa4SPACMSPACAa4SVER4.0SVER nSVER,,0Satd\Scal@Scan@TUBEC4Tmpr<UserphARphCHMphDYbigphQLcphTRphTR+|This is the confidence range *ABI_Limits..FVocAEPt<AEPt<APFN UrAPXV2APrN 1APrV1APrX}}1ARTNASPFASPt8ASPt8B1Pt7B1Pt7BCTSnBufT KmCMNT 1UCTIDKECTNMKKCTOwK]CTTL 1LCpEPDATA@DATA@DATA@DATA@DATA  FDATA DATA DATA &DATA ?~U{DATA ?~{DATA ?~Q{DATA ?~{DCHTDSamDySN 1-Dye#DyeN18DyeN1=DyeN1BDyeN1GDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FTab}%FVoc}FWO_GATCFeat//|GTyp!!1 HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mALIMS!!1bLNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISMOfSc\P1AMR^PP1RLRZP1WDRaP2AMRhFP2BAkP2RLRdPBASO_PBASMPCONYUPCONWPDMFU[PDMFMPLOCRTZPLOCRQPSZE`PTYPKePXLBRGNmK=RMXV1RMdNCRMdV1RMdXC)RPrNK RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1RunN""KS/N%M{SCAN@SMED SMLtSMPLUASPACAa4SPACMSPACAa4SVER4.0SVER nSVER,,0Satd\Scal@Scan@TUBEC4Tmpr<UserphARphCHMphDYbigphQLcphTRphTR,9drWdGY| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz9^#m{mIhp7KRV\}du-Ej,5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! K]T5^.?s|"Ff61}XTu.ycI0 r#U4rgnF.:Ryn'aTXU/7/~`}'vwVc%EI"m.9,(B~b) e:=K{_"#k<>Dj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0V8JX|K2-!;9xxsWD@|DmOY,f *"VS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt<AEPt<APFN UrAPXV2APrN 1APrV1APrX}}1ARTNASPFASPt8ASPt8AUDTB1Pt7B1Pt7BCTSnBufT KmCMNT 1UCTIDKECTNMKKCTOwK]CTTL 1LCpEPDATA@DATA@DATA@DATA@DATA  FDATA DATA DATA &DATA ?~U{DATA ?~{DATA ?~Q{DATA ?~{DCHTDSamDySN 1-Dye#DyeN18DyeN1=DyeN1BDyeN1GDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FTab}%FVoc}FWO_GATCFeat//|GTyp!!1 HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~mALIMS!!1bLNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISMOfSc\P1AMR^PP1RLRZP1WDRaP2AMRhFP2BAkP2RLRdPBASO_PBASMPCONYUPCONWPDMFU[PDMFMPLOCRTZPLOCRQPSZE`PTYPKePXLBRGNmK=RMXV1RMdNCRMdV1RMdXC)RPrNK RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1RunN""KS/N%M{SCAN@SMED SMLtSMPLUASPACAa4SPACMSPACAa4SVER4.0SVER nSVER,,0Satd\Scal@Scan@TUBEC4Tmpr<UserphARphCHMphDYbigphQLcphTRphTR