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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 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6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4abf9f0419b943be84d3fe28248ac68bSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_15-12_16543130Seq18009137720_K1319_trnhjmadera96-WellK1319_E11_Cyclanthera_trnh_trnhKB_3730_POP7_BDTv3.mobSeqPop7zy>V K [ + ) 9 > * T < : I a x q @ / h ? 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(-.)*0-#   $040$(7>9,    9Q`^O6+1.#!)+% $4:6)           %**$.:<5&    $9FH<(    #    (<GD5     ##      #()%     $5=8)    #"  ')%*<GF:' %($     '/.$        "+-' (;GH=* !$     &395) )9?7&       %+*!       0>C=0!  %/54-# &06985/( ,;DFA90*'&&'*07>?8**43&$7?;, %;JJ<$"&'68/!nA A+zAZrAFKB.bcpKB_3730_POP7_BDTv3.mobtermNNNNNNNNNN ".: ".:NNNNNBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 17:00:10 -05:00AEPt@AEPt@APFN UAPXV2APrN 1APrV1APrX}}1ARTNvASPFASPtASPtB1PtB1PtBCTS BufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA jDATA >DATA 'DATA /_UDATA /_DATA /_]DATA /_u#DCHTDSamDySN 1}Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1*EVNT1=FWO_GATCGTyp!!1\HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~ՃLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISOfSc! P1AM VP1RL LP1WD `P2AM tP2BA~P2RL jPBAS)PBAS$PCONGPCONBPDMFUPDMFPLOC 8PLOC .PSZE`PTYPKPXLBRGNmKRMXV1RMdNCiRMdV1RMdXCyRPrNK[RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1PRunN""KkS/N%SCAN@SMED SMLtSMPL USPACMSPACSPACMSVER4.0SVER SVER,,0Satd!Scal@Scan@TUBEC8Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN UAPXV2APrN 1APrV1APrX}}1ARTNvASPFASPtASPtB1PtB1PtBCTS BufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA jDATA >DATA 'DATA /_UDATA /_DATA /_]DATA /_u#DCHTDSamDySN 1}Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1*EVNT1=FTabfFVocUFWO_GATCFeat..'GTyp!!1\HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~ՃLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISOfSc! P1AM VP1RL LP1WD `P2AM tP2BA~P2RL jPBAS)PBAS$PCONGPCONBPDMFUPDMFPLOC 8PLOC .PSZE`PTYPKPXLBRGNmKRMXV1RMdNCiRMdV1RMdXCyRPrNK[RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1PRunN""KkS/N%SCAN@SMED SMLtSMPL USPACMSPACSPACMSVER4.0SVER SVER,,0Satd!Scal@Scan@TUBEC8Tmpr<UserphARphCHphDYbigphQLcphTRphTR,qJ'8f7j}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"lC+mCU1T6D3NM4MM1GWfNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Qg\c9:Y"{k$Y0Tb;KAL,kgwbPn%1eLtcUW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|G#19 g9%=;ac*K7T4`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT/RD8z{S>!lXS_ 75Hya0#C"4kyE7*r;#xjHJAEPt@AEPt@APFN UAPXV2APrN 1APrV1APrX}}1ARTNvASPFASPtASPtAUDT9B1PtB1PtBCTS BufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA jDATA >DATA 'DATA /_UDATA /_DATA /_]DATA /_u#DCHTDSamDySN 1}Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1EVNT1*EVNT1=FTabfFVocUFWO_GATCFeat..'GTyp!!1\HCFGCEHCFGHCFGHCFG==InScInVtLANE<LAST~~ՃLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISOfSc! P1AM VP1RL LP1WD `P2AM tP2BA~P2RL jPBAS)PBAS$PCONGPCONBPDMFUPDMFPLOC 8PLOC .PSZE`PTYPKPXLBRGNmKRMXV1RMdNCiRMdV1RMdXCyRPrNK[RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1PRunN""KkS/N%SCAN@SMED SMLtSMPL USPACMSPACSPACMSVER4.0SVER SVER,,0Satd!Scal@Scan@TUBEC8Tmpr<UserphARphCHphDYbigphQLcphTRphTR