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(@V\eOK- $?DKN;"MLdEG0.:UPhfL9(../ KNaLR;2LZPRVG<3KO4$89=QJa0#&4<' -7,+13O-% ');" @CC<!39:9# $(Km_]_TC6.&# ('.+-(  *7lszmY?'   44?490;"%           !"#$%&' !"#$%&',,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR SSSMMMlll   )))333=<<EEENMNWWW___gggonnwvv}}}<;;;<<<<<;;<<<<<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 19ed86dc2ff242b18a2169563162c263Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_15-12_16543130Seq18009137720_K1319_trnhjmadera96-WellK1319_C8_Senna_trnh_trnhKB_3730_POP7_BDTv3.mobSeqPop7z*:JU\dp3cT}cO A 5 . 8 i/"&)-|16@:?DIMMRmVZ_c/gUkjoAruNw)x&xSwvtrolhd`G[VQ]LFAepU)   Pe1 BX{&d"  'pIz|%R3Q` I4*h,    3_e81d){+NSB?i_lC Q#<b        Vj`r/'      !L~}M$           P^Z5"y?x|KD                        !EowL'  @ VeC?22WJs1%";mb3       Lm7  (7BJS^m}|cB"    +11))E^kiX>"  "'%  $HplH(/ZnIJu KeU';lSG'#9^b9#FqxX7 *ASZS>"!LzZ7$(Ds(Z{xO}A /Oz>MD#{H# &A_{}\8$041( "L|pN@G\s|rV4 /QyrL+DJSO-OzQ%  "! 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"!     5c{I %+,(   @zH#3AIH?1 =qZ2  *<JQMA/  ?l!%vfWG8*  9^wF.I^hf[I11|8}BQ*)>R]]Q>( %Eo}k[K;.&$(.43* 5[~jG&                                                                            %-1/')6>@<60/17<><70)#"&+/233346763.)$""#&&%#"'*($                                                                                                                                                                                                                                                                                                  Y+@A`AKAKB.bcpKB_3730_POP7_BDTv3.mobtermCAGTGACGTTTTTAATCCGCTTCTGGCTCTCCGCCCCTATACTATATATACTATATAAAAAAAATTACAGATATTTTTCATTTGTATTCTTTATCTTATGTTAAAATAATGTTAAAGAATGTTGAAGTAAATAAAAAAAATTGTATGAATATGAAGAAAAAGACTACTAAAAAAGAAAGTAAAGGAGCAATATCAAGAGGGTTGATATTGCTCCTTTACGTTCAAAAACTCATATATATTTGGACCACAATTTTATCCATTTATAGATGGAGCCTCAACCGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCCGCGACAGTGACGTTTTTAATCCGCTTCTGGCTCTCCGCCCCTATACTATATATACTATATAAAAAAAATTACAGATATTTTTCATTTGTATTCTTTATCTTATGTTAAAATAATGTTAAAGAATGTTGAAGTAAATAAAAAAAATTGTATGAATATGAAGAAAAAGACTACTAAAAAAGAAAGTAAAGGAGCAATATCAAGAGGGTTGATATTGCTCCTTTACGTTCAAAAACTCATATATATTTGGACCACAATTTTATCCATTTATAGATGGAGCCTCAACCGCAGCTAAGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAACCCGCGA&BZo} !*;O^u*2;HP\dlx *4>HR[gq|&3>IR^iu%.9DO\gr}#.8BMXcny(4?JV`ku #-8CLWamx    ' 3 = H S _ h s }   " , 5 @ K U _ j u    * 4 ? 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F7&I&!9]:45AWC\#8 5SBOO_R)+PA;Y_@F&> (<a D:0$4*T*C8 @KNU ,.!%`+2\1a6IH8-\?3d@^U <E!K6>+Q2>3$b$J#+ U\CPA^_1_(68| 'ed/ `u@V&xh`D4X=9r>th# (%QlQ$.jt6XYO- ,y)C 4<=k(LE4p *i8$f{_5oUdH+sb|>a}41tL6_!$^`% =F/gC'Dth+DOgPec &cc>gi' Wf ; N @_/LZ}wEL6xJ;g4a0i6@U,;?VJzmo2Q<$KI8%BC:>#b1%-xO?;P:C3'5  >!c  ( %<  * 'hICC &:}di+{ !,z,d8M:dY,C,S1bK98d+)plPp9'>zNNvD,4b1p8-G~-_p-p,bJyYVg;p5,p6\k%@4Au#,VoQT",&4\c K) rJ%L>Wo6UUK/z3[Y*$C!M{ld.E72r'$$=?f+#b>+G&/9,/ E1/0fEEE+ S:71Nh^<.8E^DYnC>^HL]ieVF/D'r=Sb5*A+,'/0$!6/7>(!5# +97!,%$"(+4![9*(#8T'@i]@a4"$/-.A>.F8>2(Z0*,T-6_>+;<E,63;LG^]LPE;J`7PK5:1AD&\a62jL/:e)_\_eg51>~\e5H?B0Mxm0huc=FT33XNAGAGCGGCCCCGGGGGAACACCAATCTAACTGCGTTGATATAATATATACGATATATGGGCCGTAATACCGATAGGACACACCCCTAAAAGGGGTAAGGAGAGCTTATTGTGATGGAGGGCGCCCCCCGGGAGGGCCCCGGGCGGGAGGCGAAGGAGTCCGCGACGGATCCTTGAGTTTATTTCATTTCCATAACGACAACCCCCACAAAAATGCCATGAGGTTGGGTTCAGAGGGGGAAGTACGGCGGGACGGGAGGTACGTCCTTAACGAAACTTGGGCTGCAGGTAAGGCTGTAAGGAAAGGATCGGGATTTGGGTCATGCCTAAGAGACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 16:59:46 -05:00AEPt@AEPt@APFN UqAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtHASPtHB1PtGB1PtGBCTS BufT KmCMNT 1UCTIDKECTNMKKCTOwK]CTTL 1LCpEPDATA@DATA@DATA@DATA@DATA  FDATA DATA DATA &DATA 0#`FUzDATA 0#`FDATA 0#`FDATA 0#`FvLDCHTDSamDySN 1-Dye#DyeN18DyeN1=DyeN1BDyeN1GDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FWO_GATCGTyp!!1 HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!1bLNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOIS֚OfSc\P1AMMP1RLM5P1WDMiP2AMMP2BAMM7P2RLMPBASMMPBASMMPCONMMPCONMMޛPDMFUZPDMFֱPLOCMPLOCMgPSZE`PTYPKePXLBRGNmK=RMXV1RMdNCRMdV1RMdXC)RPrNK RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1RunN""KS/N%֒SCAN@SMED SMLtSMPLUASPACMSPAC֪SPACMSVER4.0SVER SVER,,0Satd\Scal@Scan@TUBEB2Tmpr<UserphARphCHphDYbigphQLcphTRphTR!'This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN UqAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtHASPtHB1PtGB1PtGBCTS BufT KmCMNT 1UCTIDKECTNMKKCTOwK]CTTL 1LCpEPDATA@DATA@DATA@DATA@DATA  FDATA DATA DATA &DATA 0#`FUzDATA 0#`FDATA 0#`FDATA 0#`FvLDCHTDSamDySN 1-Dye#DyeN18DyeN1=DyeN1BDyeN1GDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 0EVNT 0EVNT0EVNT0FTabhFVocWFWO_GATCFeat//(GTyp!!1 HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!1bLNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOIS֚OfSc\P1AMMP1RLM5P1WDMiP2AMMP2BAMM7P2RLMPBASMMPBASMMPCONMMPCONMMޛPDMFUZPDMFֱPLOCMPLOCMgPSZE`PTYPKePXLBRGNmK=RMXV1RMdNCRMdV1RMdXC)RPrNK RPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1RunN""KS/N%֒SCAN@SMED SMLtSMPLUASPACMSPAC֪SPACMSVER4.0SVER SVER,,0Satd\Scal@Scan@TUBEB2Tmpr<UserphARphCHphDYbigphQLcphTRphTRSKbS_58LNu~)B*HUrG\W\|JLw?S15Qc4+}9~>26AEMT3pb}045@u:r*nOroz;P]]~eYA}MS8 Xo2h{/S9~i,RU`Zjp2 9c}LVFXj|udrVbH`6@m r[%`ivL&`~>`%c{nU&@:z9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,HKW>G SCe`rlx%b@cTY_eM6^sO \}!&R2{bP+*,J\]!dr.GSEu11$m&*\Q443:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;NPz;+hj3jT$3o%+0|5Q1V?yRl<`QV-AMV^@c O*Pv