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IUcZlqt,uwnxyzpzMyxvwt qnjg$cQ`[UNB6,$  G S a R  x 2?  #;`h= pZ:- 0kI?D, vuk|nkuxbnnwovwtmz~onlospyj|ltv}w{l}{k~wwYX6TSHM(?-)-*#(G" ;'#,,$$$$'%, 2'"@#'0E::3(,0%"'    "  ! &   ,      !15%-184{ehmxB (&9''hiU@B0+   2,0"()' !)3%'77%G<:F3<GRIVOCP9=ECY/<IU/M,0 # *    &$      # /4*1=AOBTN;A,HS^di|}kPB:/ *8tpPGE\OUZ^]E?0, ,$ ",K`lJC&)!14,1@- *<HLs\X    !"#$%&'()*+, !"#$%&'()*+,,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR SSSMMMlll   )))333=<<EEENMNWWW___gggonnwvv}}}<;;;<<<<<;;<<<<<;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 798dec360e634e0988151cb925878da0Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_3730NHB_2017-01-26_15-12_16543130Seq18009137720_K1319_trnhjmadera96-WellK1319_B9_Desmodium_trnh_trnhKB_3730_POP7_BDTv3.mobSeqPop7z & 9 \ f b x 3 >oDkA!#&*Q.;28Z>EMT\edmx\|jriW`XOG{?9F3/-J+** ))*+~,.l012 3578:8876420.',Q*](*%" I:&l $q_ p  | 4 v # 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" "  "   "(66.       .." '""$  &.+,-($8.$( & &"""            &     "         +.   " "  "   "(66.       .." '""$  &.+,-($8.$( & &""" Y)P=](T5 [ Oa0 Gc5H/C]]:\-?4b?N!T[KG[?BU,1R] "Xbc3 *1?,K"$P b RNNYYG )A3^ZYQA&F"X+OWU*Q%&U-99NAW *,CR%* I-3KLJ R+&  I%9']S*6 2S&]7 b ?0TFY L2:S^*:. S]U13S-O*bS6 2Y/Q' O2L(^8KI5"^.EuP&l B0{v+6k@_XdF ^ H Ma <!^I'muT73O} Nw % ~(8 ,vr> v%$'n[C_ ~HwPEXM{MfH/jT82c2-KY50%k(2w-D9WF:,3@ ,eH~F  #M?(Dji` \ o` 8h O (61Ythz<%aF[!B\ 8 ^ '?Rg<a|d VZBI:a;+73xFY-;*)8($9fpt#$pDl%8,4b92$Z@ $pRO+yopEkxLp&<U$'fA1RoduT5h7I ? ]pg}eqk@q0!,)U~IGO`Upy$#^^X@gP\.&,}l%R=nzh+H/ryu |2n58b )u;,/k6i8 :"@IDj'g2PR4&H ~ULr(q`XX -  "Hn}-D>I[=1!hwpZvm{q#H@b}vOn9r]QY^v aJe$GGAGCGCCCCCGTGAAGACACCCCATTATTAATGCGGAAAAAAATAGATCGATCATATTGGCGAAATAGGAGACGATTCCCAAATGGGAGATAGTCGTGCGGACACCGTGCGTGGATACACTTTCAGAGGTCTCAGAGGTGGTACTCAGTGTATTCTGGGATCGTAACACTTATGTCGAAATGTACAGCCATACCTTACGATAATTTTCCTCCCGGTTCCCTTGGGGATGCCCCACCAGGCAGAACGCCGTTCACAAACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-01-26 17:00:13 -05:00AEPt>-AEPt>-APFN UAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtKASPtKB1PtJB1PtJBCTSfnBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA rDATA FDATA 'DATA ?q~UDATA ?q~ԸDATA ?q~SDATA ?q~|DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1&EVNT12EVNT1EFWO_GATCGTyp!!1dHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~dLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISQfOfSc" P1AM[P1RLYP1WD]P2AMaP2BAcP2RL_PBASRPBASQPCONXPCONWPDMFUPDMFQ}PLOCUPLOCSPSZE`PTYPKPXLBRGNmKRMXV1RMdNCqRMdV1RMdXCRPrNKcRPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1XRunN""KsS/N%Q^SCAN@SMED SMLtSMPLUSPACA`]SPACQvSPACA`]SVER4.0SVER fcSVER,,0Satd"Scal@Scan@TUBEA7Tmpr<UserphARphCHQphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt>-AEPt>-APFN UAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtKASPtKB1PtJB1PtJBCTSfnBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA rDATA FDATA 'DATA ?q~UDATA ?q~ԸDATA ?q~SDATA ?q~|DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1&EVNT12EVNT1EFTabtFVoctFWO_GATCFeat//tGTyp!!1dHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~dLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISQfOfSc" P1AM[P1RLYP1WD]P2AMaP2BAcP2RL_PBASRPBASQPCONXPCONWPDMFUPDMFQ}PLOCUPLOCSPSZE`PTYPKPXLBRGNmKRMXV1RMdNCqRMdV1RMdXCRPrNKcRPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1XRunN""KsS/N%Q^SCAN@SMED SMLtSMPLUSPACA`]SPACQvSPACA`]SVER4.0SVER fcSVER,,0Satd"Scal@Scan@TUBEA7Tmpr<UserphARphCHQphDYbigphQLcphTRphTRaB$T9F_"\=QtAN-GQ6)lI PK&'Kxf]$bN8O2Rz7}062sXaA=&@X<1p3QLT1;g8kY+-^m]ZOQ>\G`HK_^_<~P[Z66YM'KvT+F4$U]]ReL#(79pA$den?R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb_v2FKeS5~i2o8xOrz_lK63<%Ri24WXXp}@c[#m*Uc WUgK:=[:"yzv+r3oJ6l&O`x Pz><3&k@EAQ/d=a-WG&OE6Oe_pSap<#2_<2EgNLf+W<#GnP Qcg*J4ot1\k|1hygS4-X;i+-'+1T:o5Q*oc0c3\EI`N^vp5cM_1h\j[<63*7wJ7{_O6?@dsgbk1^>7(FTc%{M*AqbEdG9}j~:HzpWI23Z;|?92[N:4hzRrOeMK*b0-"8/FJrlM;8'i2{js.>`AzOpPWT<;u4@"Vd*dzF4/T(wfT;GAze[6m[X1EDE?\g}_t6LPyr{;qe&Ker"&O93s'!byH:/U?vwlUQ*:UN);@J4\eym3pdZ8G%] %v*PK@|Q$OEHbX\"B-8'i2{&[y3( ]'ngg61C8dk7YZFU?-4$&LkfG;7oF`|zvn[Y<,h`+m&wE6Gz zF/"Wtixy$|FRrtlqaxkabCDk$OE-`{siO}L6iyZnsz3P<}u^ZqNX+9_e V&&OE6OLo X:QJf9/qwAxe<][#m*ZY8|k_R.AQ>UR6? sdWUx>|+)pB]M7k99HU4],2g%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tu#,xHL?iCB0'~nRnX@EV$2kE}:>&ZgL7:tl|y\@fK0\~jYA!%}CN&]n+{Ga`!bq>{*A-;Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~6>udrV0HJA@vd*W]U_I[Ddg/-AEPt>-APFN UAPXV2APrN 1APrV1APrX}}1ARTNASPFASPtKASPtKAUDTB1PtJB1PtJBCTSfnBufT KCMNT 1CTIDKCTNMKCTOwKCTTL 1CpEPDATA@DATA@DATA@DATA@DATA  DATA rDATA FDATA 'DATA ?q~UDATA ?q~ԸDATA ?q~SDATA ?q~|DCHTDSamDySN 1Dye#DyeN1DyeN1DyeN1DyeN1DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 1EVNT 1&EVNT12EVNT1EFTabtFVoctFWO_GATCFeat//tGTyp!!1dHCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~dLIMS!!1LNTD2LsrPaMCHNMODF1MODL3730NAVGNLNE`NOISQfOfSc" P1AM[P1RLYP1WD]P2AMaP2BAcP2RL_PBASRPBASQPCONXPCONWPDMFUPDMFQ}PLOCUPLOCSPSZE`PTYPKPXLBRGNmKRMXV1RMdNCqRMdV1RMdXCRPrNKcRPrV1RUND RUND RUND RUND RUNT  #RUNT ;!RUNT #RUNT :&Rate 1XRunN""KsS/N%Q^SCAN@SMED SMLtSMPLUSPACA`]SPACQvSPACA`]SVER4.0SVER fcSVER,,0Satd"Scal@Scan@TUBEA7Tmpr<UserphARphCHQphDYbigphQLcphTRphTR