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"1! &#"+ .)#,  #$!%"$    "$#(0--%0/(% ' *&+,%).*</%$$ ) &(+."&'# +%,)0%"#&#"2 $  !     ! !$<:>&,,        "#'    "   ,  %        (                                          $   }~}~ ,,,III^^r{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPOVVNNNnnn$$$...888BAAJKJSSS\\\eddmmlttt}|)<<<<<;;;<<;;;<<<;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 47d35738364e4af4ba2c69522a954527Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_21-46_17413130Seq18116137972_K1318_trnhfjmadera96-WellK1318_G11_Stellaria_ovata_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z !"#&(+-/136:?DIMQTX[`ekqw~ "*18=@@>93,&~}}}|yvrnha[UPKGC>82-)'&%%%#"  !!!  ")09CN\l)>PamttnbQ<& ygYMC:2+&" !$*19BM\o=f!>TchaO3rM) ~yslcYPG?940-+)(&&%%$$###%(,2:DPawY< ZrD z,Qwkc^[]dr-kNKejZ6kq(oXG:2,)())**+,./12210.-,+)'$ ):U}I{BL{sbFf"_F5( !#%')+++)&"  @p|n*VX2?dA.&$$%$"            "$#"   !#$"  N eo^(   """ -|UCIT   8xC+{GeD  &^+AmWTXgD$*Vo@{a=^E>AHOU[`ejptx|~|wtstx}|tj^SJC><=AEHHE?5+!    Ftf  ")*%       ,pR3g`1Y            -8(12X:         ?\e-@_    "!     !&('# %m^9\;du+               ""&g8 7i(         %kD} I1~f%L `R>hHWV'C$:!Yt+ "d79j)    Xrk T ,gc|3:   :|/2}FVAbK2K  &a"WA #^|tVGzk@S+hd8*n6   %Z lHA    _5# Sx/ F8t%r1       UbB?    !&*,,*('())'#"+H^dsjH-+5?FIMWkuaTNLNQW]ejnmh`WNIFGILMNMLKKKKMQYcnushUA7@Zyk+x~ k |JyvI B + Y 7LE+  "%')+,./13579:;:97655678752-)&$"       #'+.010/-+(%#  !!"!!"#%(+.0122334468<@CGIJKMNOPOLIEBABCEGILQZfu{naSG<3.,-2<J^z7~*a4)Xru_2;iEd)wh\SJB;40--/28?IXk5nLx^#a#kR@4-)'''''''&%#"!!""###"""#$$#!  (;Uy&pF{b6p(zV>/'#!    0R5% m%P. 'Dsu5nwSEMN0/LV]"G9!j4  0Q \ 2\uqLHZ1  5n"V?rb1  !<gA3d{n:|]&#X {E}tD8PL(nVgBG']r`.-X_; k4    GC!=o! H^@a%      ,[Q1F )S,voTWbq6?<1l:#!',17?HRZ_a`]XRLGJZ')" MU*~%XeK`-@x@#$R6< 8  4zUxZ;]M(u{d]ki/I !"!        bRRONb  2yDP;.k- \6@.w p^ -)#x<?13(. MH^ykh $w7`\   #&'&$!  !"""!  !"#$%%$#"!!"$%&%"  7Yk8H]     +m%tb3%4{] [#!Vd^gdj*xa#pUynBJ@     89 >  <GJ ; f[JzfE G<iPcZQ8X{4:|v,(N   T>H4c~yxfahdiqrxwpu\^g#  ]w1<8hgc      >B ,l)  __\m/      ,gNutLj0  SRsmLR  BL~5}7s]!   VZo"|go> I&p9   @EA ? Br6g% dXAhHhq& AdQ8 &rb{[81T  (r^*B=\ BOOH   T0 2Lf!  T&!S *5:93,%  !"""! %)++*(%!)?f41,iG7.&+DdC`>%gz&!> SK +O0 (e%    ( n m:X [:( b?l?phtuWA55:BFD</"                     $2AQbt{hWI>758@M_wR:OXTB"]+*`/{ I}s2O'$a+sU@429Jf9X :WplAN#\8!  (@aE*BF6[fA' =jPjW+O)   +P;H;QA~?(<q#CP|Y&uk4!]/S\3  B|.cUf/ @|*#@5o,(mK`LUW* F8RxrAK!)hXBuj!z;  1eWy*_ V7`fF          HlX>V.~R S~T^w1    =X PMJy6 *g2 JX2wV$  !',28<AEJPW^emv~{slf`ZRJA8/)$#$&&%!    6}SKA$Y    *y"BT# 7hG $`/ &jXT1aAC)  7] >X).bpw*  9]  Al( `>L@H          [<%[Q ?  H:[uQV>yC[-F  ]KGkd&>LJJ@]wXY  aH0\=MbUC C_t|[GK&dt^cw:  "#!      E#&ln*)HNa:v1 [+&t<  H./rr.-L  OZ[QX/0b|0        &mZ?iNfe8\lIz?]mZ(; e\=bBiu* !%&%#!K-.pk}8D8rd*TY.%T;hy- Rc`P   PIn(  $b%sT   7T1i(   &g-hJ   6i  s>  F--L     E&P Rr* D:8P#'*)'$!  8h!0gsNUz%W*"%$! "',/0/..-,("&*$iR~KN .HwlaRC70/02332221.)# 0T;$w@! !#)2>HPROH?4*""b8A9P  %A`ymS;,('  Wv 6(pf.' :G r,0#      $'*,.../0259=ADFHIJLNPRTUVVVVUUTSPNKHGFFGGGGGHIKMPRRRQQQQRRSRPOLJGDA=:7655679;=@DHLPSUUUTSQNJE?94/*%   '.6?GQ[epyxpg_XQKE=6.'"  "$(+.15:AJWet~}-kL.n5dB(AC*CNFa ]&b~En!Qymc[TPMOT`t&ls?S DF7zdVNKKMOPPOMJHEB?;7410..-,*(&$&+4CXt"VE rwY'=mzd,v DjUICCJZudHz~hYWd{V \bRThPsMc]@  3CPZ_`[TLECB@5M$nRB9420/.-,,,+*(%"!##"         @xNBo1  &^EfWPD  2r4(,L>Eo@Dm.   ,{GF`)"/.X% 7{UC7Jy:   #&+19CO\kz9Wu';LZfpx{zreS<"jK+ ~n_RE90("   $bFZpct.    $e@#]^%Iq.    ]n @-     C"JrYg%    :~=O#Hh$  9r+<Ai(  #-8CMTY]_`beiowyrkfa[TLD<6/& EIV"<SU69=%`Urlm'  hojNW   =](;!R?y9    %h<:j( (f$NJ"      0_[6 2gvA    ^P:dIqz-  `<q_Wi?   @ (Kue([hh#   RW[f4$''&&&&%%&''%!IwDhOW+'..+%!   1|SdJ   /v?T(Kj' U l%l-       QT9  1r>W0[}=$.20*$ !$'),-/.-+)(*.5>HTcyMswR"}ka\YXVUTRQONNQV^eknlhc^[XTOKNbNI,wkgc]WXc{mVG?:853358=ADFFEA6%nY8 i=S>f"zv#p6     27 AA(AYxA#$ KB.bcpKB_3730_POP7_BDTv3.mobtermGATGTCCGCATGCGCATCGTCGACTTGGCTCATCCGCCCTTTATTAATATTAATTACTTAACAAAAACGCTTAATCCGCTAGTAAAGGAGCAATACAAAATTTCTTGTTCTATCAAGAAACAAATATTGCCCCTTTACTTTTGAAACCAAAACTCGTATATACTAAACTAAAAACGAACCTTTATCCATTTGTAGATGGAACGTCAATAGCAGCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAAATCCAAGATGTCCGCATGCGCATCGTCGACTTGGCTCATCCGCCCTTTATTAATATTAATTACTTAACAAAAACGCTTAATCCGCTAGTAAAGGAGCAATACAAAATTTCTTGTTCTATCAAGAAACAAATATTGCCCCTTTACTTTTGAAACCAAAACTCGTATATACTAAACTAAAAACGAACCTTTATCCATTTGTAGATGGAACGTCAATAGCAGCTAGGTCTAGAGGGAAGTTATGAGCATTACGTTCATGCATAAATCCAA&;[f &4?KV]i}*<ETanv~(2=EO[fqz*4>HT`mw'3?KUaju $/;FQ^jv$/:FR^is~#/:EP[gs    % 1 = G Q ] h s }    % 0 ; G R ^ i t   ! ) 2 @ K V b m x &;[f &4?KV]i}*<ETanv~(2=EO[fqz*4>HT`mw'3?KUaju $/;FQ^jv$/:FR^is~#/:EP[gs    % 1 = G Q ] h s }    % 0 ; G R ^ i t   ! ) 2 @ K V b m x       /* 1:!*%&8+ &6>)3>'+>>8/>>>/>>>>>;;,; .'2---888>>>>>>>>>>>>>;>>;>>>;>;>68338.+63>>>>>;>;>>6>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>;;>>+;;>8>;>;>>>>>>>>>>>>>>>>>>>>>>>>>/>>8>>/>8,,>)         /* 1:!*%&8+ &6>)3>'+>>8/>>>/>>>>>;;,; .'2---888>>>>>>>>>>>>>;>>;>>>;>;>68338.+63>>>>>;>;>>6>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>;;>>+;;>8>;>;>>>>>>>>>>>>>>>>>>>>>>>>>/>>8>>/>8,,>)   9(a5U+JR>N J_W($Y"##`-XM`G*`C`> U%5]-+VO=94d_ 7/Y=' TRFD2 T3D;( )XL(' 9MJ C$ U:JS,H9L@/HL.W9:Y#Q^9AJ1IVA#N+U9-45/.=QM08': M4Q-DN/A &SR (#-1!7Y'?D+bF]G. *:&_J7=LCc0M@K(J"0B7Q 730 I&HD/&c4[LQ.B$8">+9aO40 6.URUnKnrMY2{ =&L#0n)?/ev/u}(]wtX[O\># )mP_q1p.rNa.*&fp)v.n~|nJ6qLJELAF:Ho^rT$$sBo76$9V*rT9 B,eS-v\f|<i2}lUw8HAOn nMJipu@VeEC@?BO=7<qk0_C]D#0(%6@$+#4-!?:,-%@9 VD            x*(ndJppppq?'q<p`d/9MB#`bi(q u,KxGr~q O5FDrd,ppYkuJ )i<1|1jHk9/z{_aI3_]1/#fw :&%40,OA,!%gM#",IB,/*Y5He 2,22&O&( ##(! 0(#"%%*$"$'2'/!'6,#&0.   FR`,#(#3/)%  "" # =79ZE>D]\Oq"A=VDuAGGCGGGCTCGTGAGCCGAAATCACGTCTAACCTGCGTTCAATAATTATAATTAATACATGACGGGCACGCATGATTCGATACCCCCTGAAGGGGGGGGTAAATCAAGCGATCACGAGGCACGCCCCCCAAAAAACCACAACTTGGGACGGGACTCGTATATACCGGAATGGGGGAGGTTCAATATTCAAATATTGATAGCACCTCCCCAACATCCCCATCCAGACTGGTGATAAGAACAACACGCACAAAGAGTCAGAGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 23:16:53 -05:00AEPt3AEPt3APFN fAPXV2APrN APrV1APrX}}%ARTNASPFASPtASPtB1PtB1PtBCTSzBufT#FCMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#FTDATA#FDATA#FDATA #}FoDATA #}F,iDATA #}FscDATA #}F]DCHTDSamDySN ½Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT REVNT ^EVNTjEVNT}FWO_GATCGTyp!!œHCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS_OfScXP1AM  P1RL  P1WD  P2AM P2BAP2RL PBASPBASPCONPCONPDMFOPDMFvPLOC PLOC PSZE`PTYPPXLBRGNmܸRMXV1RMdN ԢRMdV1RMdXԫRPrN܆RPrV1RUND RUND RUND RUND RUNT .RUNT RUNT RUNT Rate RunN""ܖS/N%WSCAN3SMED SMLtSMPL /SPACMSPACoSPACMSVER4.0SVER oSVER,,&SatdX^Scal@Scan3TUBEG11Tmpr<UserphARphCHphDYbigphQLcphTRphTR+This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN fAPXV2APrN APrV1APrX}}%ARTNASPFASPtASPtB1PtB1PtBCTSzBufT#FCMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#FTDATA#FDATA#FDATA #}FoDATA #}F,iDATA #}FscDATA #}F]DCHTDSamDySN ½Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT REVNT ^EVNTjEVNT}FTab$FVoc$FWO_GATCFeat..$GTyp!!œHCFGCEHCFGHCFGHCFG==InScInVtLANESLAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS_OfScXP1AM  P1RL  P1WD  P2AM P2BAP2RL PBASPBASPCONPCONPDMFOPDMFvPLOC PLOC PSZE`PTYPPXLBRGNmܸRMXV1RMdN ԢRMdV1RMdXԫRPrN܆RPrV1RUND RUND RUND RUND RUNT .RUNT RUNT RUNT Rate RunN""ܖS/N%WSCAN3SMED SMLtSMPL /SPACMSPACoSPACMSVER4.0SVER oSVER,,&SatdX^Scal@Scan3TUBEG11Tmpr<UserphARphCHphDYbigphQLcphTRphTR4{,|Qv%_F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBImN#'uyvHR>9Ttloml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrG]^Q|ESRDujXTE-yh;}S$oa$N;172e,qz7|!y]18`'|_n?26.?bFWJQ:Ocl2[gLY"BF(TMf:ARR42iOyqFlPBK/axJK[YlyjSu3]aC^DfF<7:cmj^sSU|MkO_b2(_CL,vnQ6?ZdwYc_`LJ6txWTikt72WevdAVs+ HtsNeOVS$|e7#?!s3xAY,CF6 ~LNJX;MM;8'i2{q6.>`A #pJE@^*\vJnPk=o?eU:g;TaywK[*oCf`y6n?2x`agv`k=;A{%i7#W yZE!e~8WY;`.pr$,.[G57'9\47.e6bZ( iu2Zv&1`2h`_`&1hE;qT%;5]U'V[,A4#O|!faiw`6wPjgvG9 HtWo0U:6%4sIe0