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'                                                ?     ) "5&0)'?.=*$<-,-(2@-3:0(G7'A&72%/ " &99A#8- '"!/   ,,,III^^r{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRPOVVNNNnnn$$$...888BAAJKJSSS\\\eddmmlttt}|)<<<<<;;;<<;;;<<<;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 70c8f164b610480c847fa95bb258eba8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-15_21-46_17413130Seq18116137972_K1318_trnhfjmadera96-WellK1318_E11_Symphyotrichum_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zjkot|".>Tn+?Vn~kS8qV@1*(('##-6=ADHLQVZ\\ZVRMF>3$umgb^ZUQNLLLMNPQSTUVUTSQNLJHFECA?<72-)'&&'''&%#! !!"""!!!  $*2:CMYi~'Nu<ZqsZ8Q$p_RIB>==AGQ_s<"J Cr/$]Ma?%xkaYSPNLJHEA>=<<=?ACGLTasA$Ru @ _x?8v bk\LNnWF;53468:;;;;<=>??>=;97530+&!#.Cd10B8I8 ufRQ[eeR+RWkL8-)(('&$#"#%'),/38=BGIJIFB=82,% .`(uzajXPKF?7/'!         <o.^VYm?P4&     #AyG]'C$ZK}@ 'iiF8VXz?     )043.% /d%&ji@   ',.+%    #+//,'  !  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Z<DP/(9'L&>2&E 4>Q ^a(SHQF`Q'32 < a)6SrA<}vk V1:1((k?<N;bmJD/<d$ju*fD/\vYD*>3S8Llg#Z"MLqG2P#ez=iy<i0_\sZ:&=O@C\ /JsE%XzZv"@ y6YvV76GUK$r hK Rrl`%,\>2N*sIvC202>`;<hT5\cTgB=; M03]~ UcOe6Z  8 o0WKB$M9-w IjqTtOzAD>=I8XK35`4N"P F@+EL<-A431&(-.$%"'5,HI#A               65   -#98G0cS%p4Upimph/8m5 _"10<,qWMC `8ZNRQn V]848U+K  JT"03Qcp 8@yAmW]&,. sH8up#|Jv*Eg,le6||4rn"$(UyXV 4k \nXp,M !:+>)dc=h=E* Q =n\9D&9-xYS(=%0.@90z4/2  (J:&!&,097B@OXG4.@IBAE1JD+<(+;k($57&?/C#$f06C%>-(%0=''26+0)0' ;B\[9EA-%1%/)N:J b3/'!%"!& 9,K%P2O'G3;<LA#"/T61*)61G.( *%-#\=2,G(+;+5"-('A_NAA0)2+\E* FUmF@<]+s`><7E#AnQj<+(W^&8XbF-O@h41Rpqa8 'DT5AGGACCCGCTCGTGAGGCCGAAATCACGTCGAACCTGCGAACTACAATAAACTATAGATATAATATATAATGAATATGAACAATCCCCCATAAGGGGGCCGCAATAGGGGCCAAGGCCGCCCCGCCCCCCTAGGGGGGGGGGGGGATAAGGCCCCTCCATAGGGGTGAGGGGGGGGGCATTAACGACCTTTAATTTCACTCTGGCCCGAAGCAGTTCTTTCTCAATAGTTTTATTAGCAAGTAAACAAAAATTAAAAACTAAAATCTCCTATACTGGTGGACTGGGACTTAACGGAAGCAGAATCCAGAAGATAATTAAGGGGCGGATGTTCCCCAGAGACAAAGGGAATGAGAATAAGAAGCTCCCACGCGAAGCAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-15 23:16:56 -05:00AEPt3AEPt3APFN APXV2APrN ¼APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSUBufT#F܉CMNT …CTID`CTNMfCTOwyCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#FDATA#F:DATA#FDATA *TTDATA *TT9DATA *TT^DATA *TTDCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFWO_GATCGTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~T LIMS!!’LNTD2LsrPaMCHNMODF ³MODL3730NAVGNLNE`NOIS7OfSc (P1AM{FP1RL{CP1WD{IP2AM{OP2BA{{RP2RL{LPBAS{{9dPBAS{{7PCON{{BFPCON{{@PDMFPDMF7PLOC{=PLOC{:PSZE`PTYP܁PXLBRGNmXRMXV1RMdN BRMdV1RMdXKRPrN&RPrV1RUND RUND RUND RUND RUNT .RUNT RUNT RUNT Rate 0RunN""6S/N%7SCAN3SMED SMLtSMPLSPACMSPAC7SPACMSVER4.0SVER USVER,,Satd (^Scal@Scan3TUBEE11Tmpr<UserphARphCH7phDYbigphQLcphTRphTR*uThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ¼APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSUBufT#F܉CMNT …CTID`CTNMfCTOwyCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#FDATA#F:DATA#FDATA *TTDATA *TT9DATA *TT^DATA *TTDCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTabcFVoccFWO_GATCFeat//cGTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~T LIMS!!’LNTD2LsrPaMCHNMODF ³MODL3730NAVGNLNE`NOIS7OfSc (P1AM{FP1RL{CP1WD{IP2AM{OP2BA{{RP2RL{LPBAS{{9dPBAS{{7PCON{{BFPCON{{@PDMFPDMF7PLOC{=PLOC{:PSZE`PTYP܁PXLBRGNmXRMXV1RMdN BRMdV1RMdXKRPrN&RPrV1RUND RUND RUND RUND RUNT .RUNT RUNT RUNT Rate 0RunN""6S/N%7SCAN3SMED SMLtSMPLSPACMSPAC7SPACMSVER4.0SVER USVER,,Satd (^Scal@Scan3TUBEE11Tmpr<UserphARphCH7phDYbigphQLcphTRphTR4{,|Qv%F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBImN#'uyvHR>}TgErml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MrrG]^QXfaX)~i-DcUPO6K3Dodx"?Lq9Jv2@ Ki6t{_"#kt72We<>/D?*Q| -4KD@|Q$OEH5 G,90lVinJilT>r~+:mzv&1`Z=c':Z^j?Fe6bZ( %P^gWG^D(dWKO_^h\)SQQK=`x&i9me/%ToN668d#VihGJNMhpz<2:}MKlQ)kA.=HTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gW>i;{nI YI=pI}JIXEG%w8.2_/ KRZHlEDx8_1tm9it=9SH,]!:h^_mdZ=y$Ib4phK^M\}:"iPS!T/RD8z{&]t[XSXW .CANq~Q9U,Z:X=<;'4"C\|J_@A[]"TM,s"6rg8Z~9}__';_/x"ieGlhx6m}PzN?29zxhW)!hs|*>H~?p MD8zEXTMk|k.j H5Xv&~^\mcJT&1o?e3{0+VNwBx6Ks;6R&8yT,I}_7~j{nF?/aOw2gpO0,cW`GCYHs@dUa0u:W^$)P? sI}}1%j-]Tsz^-d+O'i~88u h`k@_[O~}<"&eG1<@O(LxCmqL6&pg;gjmMUwch[}k@_ajF= =&e=.7I 9'k0WC.%oXB{H3<(}{Uw2yy>T3pb}0Zz>zWvW9/AEPt3AEPt3APFN APXV2APrN ¼APrV1APrX}}ARTNASPFASPtASPtAUDTsB1PtB1PtBCTSUBufT#F܉CMNT …CTID`CTNMfCTOwyCTTL |CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA#FDATA#FDATA#F:DATA#FDATA *TTDATA *TT9DATA *TT^DATA *TTDCHTDSamDySN ]Dye#DyeNhDyeNmDyeNrDyeNwDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNT EVNTFTabcFVoccFWO_GATCFeat//cGTyp!!<HCFGCEHCFGHCFGHCFG==InScInVtLANEWLAST~~T LIMS!!’LNTD2LsrPaMCHNMODF ³MODL3730NAVGNLNE`NOIS7OfSc (P1AM{FP1RL{CP1WD{IP2AM{OP2BA{{RP2RL{LPBAS{{9dPBAS{{7PCON{{BFPCON{{@PDMFPDMF7PLOC{=PLOC{:PSZE`PTYP܁PXLBRGNmXRMXV1RMdN BRMdV1RMdXKRPrN&RPrV1RUND RUND RUND RUND RUNT .RUNT RUNT RUNT Rate 0RunN""6S/N%7SCAN3SMED SMLtSMPLSPACMSPAC7SPACMSVER4.0SVER USVER,,Satd (^Scal@Scan3TUBEE11Tmpr<UserphARphCH7phDYbigphQLcphTRphTR