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"&('.>'  %$4         &/+,6"&    "       9 &#** !' $#"<=T)@>=A04(*AUIB?=CHILIL>DHT>!566:2*H@814$/+ !' -& >CDEFGHIJKLMNCDEFGHIJKLMNd,,,III^^^{{{&&&999DDDXXXaaavvv      ***333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR<QPOMMMlll   '((111;;;DDDLMLUUU]]]efemmmuuu|||<<<<<<<<<<<<;;<<<<;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 4569e6027a8b48c7ac675c0089b838bdSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_23-18_17243130Seq18095137934_K1322_K1318_trnhjmadera96-Well&K1322_G10_Selaginella_pallescens_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z-8AJUct"4GZmnY@(hR@3+'%"&.48:>EO[ht|{unf\PA1 yrlihjloppomkklnpsuvurokgd`]YTPMLKKJF@:41025652-)&%'+.010-*'%$%&'(('$!!#%%%%%(,29AKVdu -Rx.EV`dbYJ5hByfXOIEA??BHR`r @{RI=t1FPT+ {slfb]ZVRMHDA>=<<=>?@ABBBBEKTaq >yJ<$]`.c}5Y4|ocXPIC>:74212368999:<@EJORRRQPQTX\agqY&f[>x^LB<99888;AM_yEpb<;m7 oK"IbM>4-(%#"!!  $.@Z}`9vH[mO;.'%$&(*+)'%$$&(+,,*(&%&+4CZ|5M-JI*@{"lL8-&# !,Ac%R9SL%%XzQ7'      FS\YjeRT.  %Ml x(<0|Vp3  ;yWaT=g/                #Up0biB!j1  :{RXiiO(&,26:<>@ABDFIMRX^cefda^ZY\h^O3"|xkimrwz||zvph`YTTZi(ry&Y1  ,Wq]r)AcoA4&4|}8;    #)--)" &swD>LmL $))%  "UgZO  $%# 9t,-?"y X#8s4]x[ $B904_$    "U Lyo2Ty4   BY8)X   9oDrl8e3  !  /Hn.v4h"W~Q|2  !!   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(Sl> @87P$  .tIL> 'X%OZCO% !V\v%sHyf'u{Ej F!  ,itjk2G-oh$I "WKZq9H0A3 Z/ 8i+%cTh.dyh8h8  %V9t[L'  @zYOwC    'V$Wl`5qA"   C|@^Z7|=H5 a:!    4h<<sC"     #PNf]7P/   $T+azoEP2# !"  .p ?L3dTQTTND:2--...+($!!#%')+-.///.+)&%(2Ee9e~i=gK>>GUcmqog[L=0%!##!&MT+kxKXq4|`NIP^mushWD3%     ; A A1_pAbA A'KB.bcpKB_3730_POP7_BDTv3.mobtermAAGTGCCGCATGACGACATCGTCTTGCTGGCACGATCCGCCGCCCTCCCCGTCTGCTCTGTACAATCCAATTACTATTTATATTCGTCCCGAACGAAGAGGGTATAATGACCTTAGAAGAGGAACGACGACGAAGGTTCGTCCTTCTTCCTTCTAAGGTCCATTACCACGACCCGATATATTGGGGTCCCAGTCGCAGCTCGGAGTGCCAGAACTAGAATAATGAAATGCTGACTCCTGCACCGATGGCGACTACTAACGACCCATCGGCATCATGCTAGTTAGCCATTTAGAGAAGGAACTTCAACGGAAGCCAGATCTAGAGGGAAGTTGTGAGCATTACATTCATGCATAACTGCAAAGTGCCGCATGACGACATCGTCTTGCTGGCACGATCCGCCGCCCTCCCCGTCTGCTCTGTACAATCCAATTACTATTTATATTCGTCCCGAACGAAGAGGGTATAATGACCTTAGAAGAGGAACGACGACGAAGGTTCGTCCTTCTTCCTTCTAAGGTCCATTACCACGACCCGATATATTGGGGTCCCAGTCGCAGCTCGGAGTGCCAGAACTAGAATAATGAAATGCTGACTCCTGCACCGATGGCGACTACTAACGACCCATCGGCATCATGCTAGTTAGCCATTTAGAGAAGGAACTTCAACGGAAGCCAGATCTAGAGGGAAGTTGTGAGCATTACATTCATGCATAACTGCA)J\b}'6BM[cnz%08APZcs '0?GXbjt -7CMZht ",9FS^ht $.;IT]iw"-:GT`my(5ALXcnz  $ 1 > J U ` l v    , 7 B M X d q }    ( 4 ? 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K W d n z    ' 4 = I T _ k v  ! , 7 B M Y e p { &1<GS]gr~&1=HS_jv'1:FOWes     $&:1 -#'&-:!*/>)5/&)>>! //>> & )'>>>8>>>>6)/>>6>/#>///>/,,>'* //,>>>1>/1>>>6>>>>>>>/>>6>/>>>>1>>>>>1/>>>61>>>>>>>>>>>>>>>//>>>>6>6>>>)%/>>>>>>>6>>>1>>>>>>>>>>>>>>,,>>>>>>>6>>6>>>>>//>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>63>>>>>>.>>>8>>>>>>888/      $&:1 -#'&-:!*/>)5/&)>>! //>> & )'>>>8>>>>6)/>>6>/#>///>/,,>'* //,>>>1>/1>>>6>>>>>>>/>>6>/>>>>1>>>>>1/>>>61>>>>>>>>>>>>>>>//>>>>6>6>>>)%/>>>>>>>6>>>1>>>>>>>>>>>>>>,,>>>>>>>6>>6>>>>>//>>>>>>>>6>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>63>>>>>>.>>>8>>>>>>888/  5=B=ZZ6 <=NJQ *BG!ACXSIA7cO*XXT'Y,J HDKE Z5M=QC>J$U9/;1YQXP K-DBPD!@O2>Y C#N$B;232^)T/5+c=ANTN:cM)QZY:H.B7H&I_ /L" #, / 'C0'UN.J: `*"29$NM.*X]-JC!<:S0(Y4-Q?b.@Y JH@.X K aG )%Xb@FS4=,a C&;`:;F:&"O#=MN726$FMME%EK72 J^4K:/),U%:G N*)G<@-"-)E\G^S48A,&1$HI2N>-IQIA%_<=' *a# n%8nPC^_hB+ #.]kA>/d6 i/ct:4!O/xNLWpl705|$*py; 4R+-p~/7Qe`%J8*H$y+h0CJK@yn,|3 g6 ]PGl09j1,YI\fR5X7ZSGnk8emxi~en.I1P@]47|_f*X*  x=/ \*` 3-c TeIeo}vrpfldqjLPC}z1=PFAK-&.J=C)+?=AVBsu<`HBd/'((,(2) "-' " )%#""B:!2*/"          # !                                &    6 G L2i_3j:Vp$}JZ,p| aInLp SpsIpIdAB!`S lknIp,JE^; ueNWp}!p7P /,Uk q\pWM"AQ1 tnnUphpBv670(D%ZV , z7v.8l," "3cY'&0q7SU$+$+zJ`_[ "4"<* Vmg|CL%!4)#("L0 J#O:`S?.-*' % #%#?!"%0(!0&" $ 4-"@"bIX")&$$!#)F/(5"<1!( !DZ&6$ $( A416/*&&)1/ ?-/C=.*).$'"G+)..'&F #-N1] DxR=rCB~WRU] 6@GGAGCGACTCGTCGAGACCGAAAACCGGTCACACGCTGCGGCTTTCTTTGCCTGTGCTGTACACTCTACGAATAATAAATATACTCCTTTTTGAAGGAGACTATATGGACATCAGAGGAGATTCACCACCTTGGAAACTCCTTAATCCTTACTGTGAACTTCAATTTCTCGGTTCGAGACAAAAAAATTTCAAGCGGAAAACAGACCGGCAGGACGACTATTAAGGTAAGAAGGATTCATCATCGGTCGACAGGAGGGAACTTTCATATTTATTGTGATTGAGCTTCGAAGAGAGGAAGCACCTGGTCCGGATTCCTAACCAGATAGGAGATGTGAATAATAGACTCAAACATTAAAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-11 00:49:05 -05:00AEPt3AEPt3APFN APXV2APrN <APrV1APrX}}EARTNASPFASPtzASPtzB1PtyB1PtyBCTS* BufT'NCMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'N\DATA'NDATA'NDATA %JDATA %J/DATA %JyDATA %JãDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT rEVNT ~EVNTŠEVNTFWO_GATCGTyp!!¼HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~(LIMS!!LNTD2LsrPaMCHNMODF 3MODL3730NAVGNLNE`NOIS OfScXP1AMgP1RLgP1WDgP2AMg$NP2BAgg'P2RLg!PBASggEPBASgg PCONggPCONggHPDMFPDMF PLOCgzPLOCgPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܦRPrV1RUND  RUND  RUND  RUND  RUNT  RUNT 0"RUNT )RUNT 0 Rate °RunN""ܶS/N% SCAN3SMED SMLtSMPL''\SPACMSPAC SPACMSVER4.0SVER *SVER,,FSatdX^Scal@Scan3TUBEB10Tmpr<UserphARphCH phDYbigphQLcphTRphTR/YThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN <APrV1APrX}}EARTNASPFASPtzASPtzB1PtyB1PtyBCTS* BufT'NCMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'N\DATA'NDATA'NDATA %JDATA %J/DATA %JyDATA %JãDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT rEVNT ~EVNTŠEVNTFTab8gFVoc8VFWO_GATCFeat//8'GTyp!!¼HCFGCEHCFGHCFGHCFG==InScInVtLANENLAST~~(LIMS!!LNTD2LsrPaMCHNMODF 3MODL3730NAVGNLNE`NOIS OfScXP1AMgP1RLgP1WDgP2AMg$NP2BAgg'P2RLg!PBASggEPBASgg PCONggPCONggHPDMFPDMF PLOCgzPLOCgPSZE`PTYPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNܦRPrV1RUND  RUND  RUND  RUND  RUNT  RUNT 0"RUNT )RUNT 0 Rate °RunN""ܶS/N% SCAN3SMED SMLtSMPL''\SPACMSPAC SPACMSVER4.0SVER *SVER,,FSatdX^Scal@Scan3TUBEB10Tmpr<UserphARphCH phDYbigphQLcphTRphTR9pL)EouGd+O'i~o4!KC_K:Zyr-}JIPSX 3-k>"A;%OM5/YME5ts_{'#=g;PFi147nM6!TZ7m8Q`Kp>?0T4`FI57%6C}:*SM|aZL."zr~Mu^yc&w*{we*S#|Y;;y+Y3y'E3ro{`qaL`hlZZdBc'@wMXC5K;@WJG7jVlG0$_9'mSTve]^g:G1|*dc0U+O}L6iyNmwP@rB ]:l=&3#Veqy{g_hD6z\[N.6| VkycEh,MR}*D]d0ov{dk@ /kXSE@a!)D$SXtmI^MHgX63g4`mA^.?c`fUw3^NU>xW#nZE35ux2]DbhLYr4EjQI}>uDs5iqbyMimE1ZDh7BjLh|2`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pMCWg>6SSNM44NJh7QAI8p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQn,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*