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V? uXJ=FTC`~f1bU.SqV\8?48G`d4 g rk.C l "@A j j >7gKU1T^tPe +]QqsweMOUV/K:H)3@'6*0!$    "%&) $ "    &                          & #$2(- %' %%    0j  !" !"d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333>G>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR<QPOMMMlll   '((111;;;DDDLMLUUU]]]efemmmuuu|||<<<<<<<<<<<<;;<<<<;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 1627b86febbf4bdfb79a66d8592c5fccSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-02-10_23-18_17243130Seq18095137934_K1322_K1318_trnhjmadera96-WellK1322_F3_Urera_verrucosa_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z,;HVi1Pq *7CMWbnxwk\J6}]@% pbWOJGFINV`kvygTB/zsmihhjmopqqpppqppomkifc_[WRNIEA>;9630,)'&'(*++*)('&%$!!#&).5>L]t M~Mzf1PzhYNGCBDKTbs-n i/U78 Z>+Z*zqjc\UOJFCA?>=<<;<?DMZl@"x.'bn8@*O|vv|%g g%{  }*oj#oZMD?;976543322111110.,)%" ':Yhb-<$BT(  }5KrS>0($##$#"! !$'+.01211100.*%   "##! 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V9 '.U   "%')*++,-...-+*('%%$%'()*)'%$"!      ?PjZ! b=hd dv;9Q "#         (j%vg_%   B\P> Jy M  0n ]x-  `I H!   G%S     KDwYk/       #`n_R7Ubs$t6     *e=XJD   V\& ##M qdR"  H1dqSz?   'j.DF  8~5hj1     Q;jpM r:       UKwvJ`-   !% 9HH"lK=;<92( "##!  D| Gnv\)nF/%##" !-=LZab[OA3( #hJB.~ ~U6$&2BSahg\L9(%-23.&  v A^A"W?AQhsA KB.bcpKB_3730_POP7_BDTv3.mobtermATGTGACCTCGCGAATCGTCGACTTGGCTCATCCGCCCTATACTATATAACAGTATTAGGAAAATAGACTCTAATTTATAGTAATTAATACTTCAATTTACAATACAACGTTAATAAGAATTTTTTTTTTATTTTGTAACATAAACAAATTATTATTATTAACGAAAAGGAGCAATACTTACATTCTTGACAGAAAAACGTAAGTATTGCTCCTTTATTTTTCAAAAACTCGTATACACTAATCTAATATAGCGGTATTATCCATTTGTAGATAAGACTTCTACAGCAGCTAAGTCTAGAGGAAAATTGTGAGCATTACGTTCATGCATAACCAAATGTGACCTCGCGAATCGTCGACTTGGCTCATCCGCCCTATACTATATAACAGTATTAGGAAAATAGACTCTAATTTATAGTAATTAATACTTCAATTTACAATACAACGTTAATAAGAATTTTTTTTTTATTTTGTAACATAAACAAATTATTATTATTAACGAAAAGGAGCAATACTTACATTCTTGACAGAAAAACGTAAGTATTGCTCCTTTATTTTTCAAAAACTCGTATACACTAATCTAATATAGCGGTATTATCCATTTGTAGATAAGACTTCTACAGCAGCTAAGTCTAGAGGAAAATTGTGAGCATTACGTTCATGCATAACCAA &ATht "-6?ERdw"+;CNW`kt &/9BKUbox (4>IS^iu !-8DQ[fp{ !+7BMZdnx%0<FQ]hr}  ,8CNXco{    ( 3 > I S ] h u    * 5 @ J T ` k v   ) 4 @ J V b l v    * 4 @ K T ^ i u     ( 3 > I T _ i s } !)7CNYdny &ATht "-6?ERdw"+;CNW`kt &/9BKUbox (4>IS^iu !-8DQ[fp{ !+7BMZdnx%0<FQ]hr}  ,8CNXco{    ( 3 > I S ] h u    * 5 @ J T ` k v   ) 4 @ J V b l v    * 4 @ K T ^ i u     ( 3 > I T _ i s } !)7CNYdny       !4&%33*3>>/8*,;>> 1>+;>;+>>>>>>>;>;;> 21;8+1 3>>>>>>1>>>>>>>>>;>>>>>>>>;>220"""2;;>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>;>>>>>>>>;>>>>>>>;>;>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>;>>>>>;;>>;>>;;>;;>;;>8>>;>;>>>>>>11>;1;>>>>;>>;+;; 22$        !4&%33*3>>/8*,;>> 1>+;>;+>>>>>>>;>;;> 21;8+1 3>>>>>>1>>>>>>>>>;>>>>>>>>;>220"""2;;>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;>>>>>>>>>>>;>>>>>>>>;>>>>>>>;>;>>>>>>>>>>>>>;>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;;>>>>>>>>>>>>>;>>>>>;;>>;>>;;>;;>;;>8>>;>;>>>>>>11>;1;>>>>;>>;+;; 22$ XJ0\".)#!&NSK '#O-]W@ +ZNGF#\`Z@)U0.9/']O$HS@(D5VC*+)+AT>4JC)Q/)FC/LRU!D( G5#=E $=%$JGdR--E[ :5:\'=7Ca;9-W [?-U3L\+/`67TKW Y;C)R /B_bdV\)/#$(\6%H3P?I=5(! [+Z' _G0 _U>7!P8 IXD)I?92 #K @W@)X^)UZ7!AT-Z(;!GK8>F*SSS],%QS NAT6;OR8J.%;W&S#S$-0E5c.N :aO9O/OXPU)a( Hs |-TlL8`=s1sH9.*RzoudwD+H>vzM\ *7}sG.w 3rc $qm)`C;XPo>*ZIfHPBAeAM/co)rzklvPw uL\0l_F&6**/$o9LhwL<z(iXDS-><u$ >>{ -?n}o((!} g!dYA[=@ m~YlTX_@f9@};KV*J10L3Hx\)1+#**   !#0     xuH,BapwppnppZZiDCD^9EF ljld2 |g'_E|GG\&dhC U:ddB'p [ RN4R8c(Md0y&w8,!pw.\pVpQ2B`o |MLn%Nn!xIeR)I]`aa   2-9+CE+OWX6)#=E'<!5  $MG%D)F21;3,7! 9   '& 2 .,"4$D+0(!  2!>G@=!<&$=& &? ,3!*@0 )",%E%.C-0uEk%T'c**'$a""E81 %**J4+,et^Jo7(GGCGCCGTCGAGAGCCAAAATCACGTCGAACATGCGTTATACAATATATCACAATAATAAGGGTATTCACGCCTAAATATCCCCCCTTAGAGGGCCCCACACGGGACCGCGATTACGAGGACCCCCCCCAGAAGGTGGGACCCGCACCTACTCCTACTACGGGCGGGGTTGCGCGACTCCCACCCTCACGACAGGGTGTCGTCCCTAGTGCTTCGCTAACCCACCGGCTCGCGTCCACTCCGATCCGGGGGAGCTCCAATATTCGGCCAGATATGACACCTCCACAGCCTCCCAATCGAGAAGGGTAATGAGAGCACTTCGCTCAAAGGCCAACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-02-11 00:48:51 -05:00AEPt3AEPt3APFN JAPXV2APrN APrV1APrX}}ARTNASPFASPtOASPtOB1PtNB1PtNBCTS<BufT'NCMNT ½CTIDܘCTNMܞCTOwܶCTTL ´CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'NbDATA'NDATA #GRSDATA #GR,DATA #GRsDATA #GRIDCHTDSamDySN •Dye#DyeN DyeN¥DyeNªDyeN¯DyeWDyeW8DyeWSDyeWgEPVt!4EVNT *EVNT 6EVNTBEVNTUFWO_GATCGTyp!!tHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfSc 4P1AMOP1RLO NP1WDOP2AMOP2BAOOdP2RLO(PBASOO%PBASOOPCONOO PCONOO PDMF3PDMFPLOCOPLOCOtPSZE`PTYPܾPXLBRGNmܐRMXV1RMdN zRMdV1RMdXԃRPrN^RPrV1RUND  RUND  RUND  RUND  RUNT  RUNT 0"RUNT )RUNT 0 Rate hRunN""nS/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER 1SVER,,Satd 4^Scal@Scan3TUBEA3Tmpr<UserphARphCHphDYbigphQLcphTRphTR JThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN JAPXV2APrN APrV1APrX}}ARTNASPFASPtOASPtOB1PtNB1PtNBCTS<BufT'NCMNT ½CTIDܘCTNMܞCTOwܶCTTL ´CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'NbDATA'NDATA #GRSDATA #GR,DATA #GRsDATA #GRIDCHTDSamDySN •Dye#DyeN DyeN¥DyeNªDyeN¯DyeWDyeW8DyeWSDyeWgEPVt!4EVNT *EVNT 6EVNTBEVNTUFTab+FVoc+FWO_GATCFeat//+WGTyp!!tHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfSc 4P1AMOP1RLO NP1WDOP2AMOP2BAOOdP2RLO(PBASOO%PBASOOPCONOO PCONOO PDMF3PDMFPLOCOPLOCOtPSZE`PTYPܾPXLBRGNmܐRMXV1RMdN zRMdV1RMdXԃRPrN^RPrV1RUND  RUND  RUND  RUND  RUNT  RUNT 0"RUNT )RUNT 0 Rate hRunN""nS/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER 1SVER,,Satd 4^Scal@Scan3TUBEA3Tmpr<UserphARphCHphDYbigphQLcphTRphTR&T`XDG7IG!Hgn+1Ab;*55p]?'KzK:*]Q{Y^@>=,8,XNKioh|m C]Z&|k1jK4|<0S1pPRuKojmqy=Y`gP#0beaA({]/wQ2a52rW.X}=1m)h>VO@0$^tc:"R/7J0jVgSM0=AO5+znJO!p8Npd-(bcnP6GZ]#(lCKOMQw T"S2Mmn[Ca,k +9}WQ@UzbaI23ttrRXc|LVn!<|'kU$3g;Z6Obl% eIe5_@{&]t[XS_Q7u2&q6X+9mvH%lFGTF4kZ7#+hc>b.I3u\oi$uRNBr4ylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfKeb[1!lqXV?}AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnPk=o?eUKGM5J_bi(l{~Gj`X0?888%g^^7FDg;AEPt3AEPt3APFN JAPXV2APrN APrV1APrX}}ARTNASPFASPtOASPtOAUDT;jB1PtNB1PtNBCTS<BufT'NCMNT ½CTIDܘCTNMܞCTOwܶCTTL ´CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'NbDATA'NDATA #GRSDATA #GR,DATA #GRsDATA #GRIDCHTDSamDySN •Dye#DyeN DyeN¥DyeNªDyeN¯DyeWDyeW8DyeWSDyeWgEPVt!4EVNT *EVNT 6EVNTBEVNTUFTab+FVoc+FWO_GATCFeat//+WGTyp!!tHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfSc 4P1AMOP1RLO NP1WDOP2AMOP2BAOOdP2RLO(PBASOO%PBASOOPCONOO PCONOO PDMF3PDMFPLOCOPLOCOtPSZE`PTYPܾPXLBRGNmܐRMXV1RMdN zRMdV1RMdXԃRPrN^RPrV1RUND  RUND  RUND  RUND  RUNT  RUNT 0"RUNT )RUNT 0 Rate hRunN""nS/N%SCAN3SMED SMLtSMPLSPACMSPACSPACMSVER4.0SVER 1SVER,,Satd 4^Scal@Scan3TUBEA3Tmpr<UserphARphCHphDYbigphQLcphTRphTR